Genetic diversity of the iron‐binding protein (Fbp) gene of the pathogenic and commensal Neisseria
The pathogenic Neisseria and most commensal Neisseria species produce an iron‐binding protein (Fbp) when grown under iron‐limited conditions. In the current study, we confirmed the presence of Fbp, as well as DNA sequences homologous to the gonococcal fbp, in strains of N. gonorrhoeae, N. meningitid...
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| Veröffentlicht in: | FEMS microbiology letters 1994-02, Vol.116 (2), p.123-129 |
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| creator | Genco, Caroline Attardo Berish, Sally A. Chen, Cheng‐Yen Morse, Stephen Trees, David L. |
| description | The pathogenic Neisseria and most commensal Neisseria species produce an iron‐binding protein (Fbp) when grown under iron‐limited conditions. In the current study, we confirmed the presence of Fbp, as well as DNA sequences homologous to the gonococcal fbp, in strains of N. gonorrhoeae, N. meningitidis, N. cinerea, N. lactamica, N. subflava, N. kochii and N. polysaccharea. The fbp genes from these strains were amplified by the polymerase chain reaction, digested with StuI or RsaI, and the restriction patterns examined. The patterns for the gonococcal and meningococcal fbp were virtually identical; however, variations were observed in the fbp sequences of the commensal Neisseria species. N. flavescens, N. mucosa, N. sicca, N. ovis and Branhamella catarrhalis, did not produce Fbp as detected by sodium dodecyl sulfate‐polyacrylamide gel electropheris and reactivity with an Fbp specific monoclonal antibody, nor did they hybridize to an fbp‐specific DNA probe. |
| doi_str_mv | 10.1111/j.1574-6968.1994.tb06690.x |
| format | Article |
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In the current study, we confirmed the presence of Fbp, as well as DNA sequences homologous to the gonococcal fbp, in strains of N. gonorrhoeae, N. meningitidis, N. cinerea, N. lactamica, N. subflava, N. kochii and N. polysaccharea. The fbp genes from these strains were amplified by the polymerase chain reaction, digested with StuI or RsaI, and the restriction patterns examined. The patterns for the gonococcal and meningococcal fbp were virtually identical; however, variations were observed in the fbp sequences of the commensal Neisseria species. N. flavescens, N. mucosa, N. sicca, N. ovis and Branhamella catarrhalis, did not produce Fbp as detected by sodium dodecyl sulfate‐polyacrylamide gel electropheris and reactivity with an Fbp specific monoclonal antibody, nor did they hybridize to an fbp‐specific DNA probe.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1994.tb06690.x</identifier><identifier>PMID: 8150256</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Antibodies, Bacterial ; Antibodies, Monoclonal ; Bacterial Outer Membrane Proteins ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - immunology ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Blotting, Southern ; DNA Probes ; DNA, Bacterial - analysis ; Fbp ; Fundamental and applied biological sciences. 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In the current study, we confirmed the presence of Fbp, as well as DNA sequences homologous to the gonococcal fbp, in strains of N. gonorrhoeae, N. meningitidis, N. cinerea, N. lactamica, N. subflava, N. kochii and N. polysaccharea. The fbp genes from these strains were amplified by the polymerase chain reaction, digested with StuI or RsaI, and the restriction patterns examined. The patterns for the gonococcal and meningococcal fbp were virtually identical; however, variations were observed in the fbp sequences of the commensal Neisseria species. N. flavescens, N. mucosa, N. sicca, N. ovis and Branhamella catarrhalis, did not produce Fbp as detected by sodium dodecyl sulfate‐polyacrylamide gel electropheris and reactivity with an Fbp specific monoclonal antibody, nor did they hybridize to an fbp‐specific DNA probe.</description><subject>Amino Acid Sequence</subject><subject>Antibodies, Bacterial</subject><subject>Antibodies, Monoclonal</subject><subject>Bacterial Outer Membrane Proteins</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>DNA Probes</subject><subject>DNA, Bacterial - analysis</subject><subject>Fbp</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial - genetics</subject><subject>Genetic Variation - genetics</subject><subject>Genetics</subject><subject>Iron acquisition</subject><subject>Iron-Binding Proteins</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Neisseria</subject><subject>Neisseria - genetics</subject><subject>Neisseria gonorrhoeae</subject><subject>Neisseria gonorrhoeae - genetics</subject><subject>Neisseria meningitides</subject><subject>Neisseria meningitidis - genetics</subject><subject>Periplasmic Binding Proteins</subject><subject>Polymerase Chain Reaction</subject><subject>Restriction Mapping</subject><subject>Sequence Analysis</subject><subject>Species Specificity</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc1u1DAQxy1EVZbCIyBFCKFySBh_xLF7QKoqtlRa4AJny3EmrVf5WOwsdG88As_Ik-DVhr1WtSWP5Pn9x-P_EPKaQkHTer8uaFmJXGqpCqq1KKYapNRQ3D8hi2PqKVkAr1ROQVfPyPMY1wAgGMhTcqpoCayUC9Jc44CTd1njf2KIftplY5tNd5j5MA5_f_-p_dD44TbbhHFCP2Tny3rzLrtNqv_gxk53Y7pIRezQZG7sexyi7bIv6GPE4O0LctLaLuLLOZ6R78uP364-5auv1zdXl6vcCaZ53nLdagYoS1FSzdJumvQ7UC4dNapG2wqYq2qGDiioBqpScNli20oQ4PgZeXuom5r9scU4md5Hh11nBxy30VRSMKmUeBCkyT8hFEvgxQF0YYwxYGs2wfc27AwFs5-FWZu94WZvuNnPwsyzMPdJ_Gp-ZVv32Byls_kp_2bO2-hs1wY7OB-PGNeVLIEn7MMB--U73D2iAbP8vKKM83_cE6aN</recordid><startdate>19940215</startdate><enddate>19940215</enddate><creator>Genco, Caroline Attardo</creator><creator>Berish, Sally A.</creator><creator>Chen, Cheng‐Yen</creator><creator>Morse, Stephen</creator><creator>Trees, David L.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19940215</creationdate><title>Genetic diversity of the iron‐binding protein (Fbp) gene of the pathogenic and commensal Neisseria</title><author>Genco, Caroline Attardo ; Berish, Sally A. ; Chen, Cheng‐Yen ; Morse, Stephen ; Trees, David L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4293-f39f920e6545192929dd99408c940be8d9a702c7b2ec0108d075436feff6040c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Antibodies, Bacterial</topic><topic>Antibodies, Monoclonal</topic><topic>Bacterial Outer Membrane Proteins</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>DNA Probes</topic><topic>DNA, Bacterial - analysis</topic><topic>Fbp</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial - genetics</topic><topic>Genetic Variation - genetics</topic><topic>Genetics</topic><topic>Iron acquisition</topic><topic>Iron-Binding Proteins</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Neisseria</topic><topic>Neisseria - genetics</topic><topic>Neisseria gonorrhoeae</topic><topic>Neisseria gonorrhoeae - genetics</topic><topic>Neisseria meningitides</topic><topic>Neisseria meningitidis - genetics</topic><topic>Periplasmic Binding Proteins</topic><topic>Polymerase Chain Reaction</topic><topic>Restriction Mapping</topic><topic>Sequence Analysis</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Genco, Caroline Attardo</creatorcontrib><creatorcontrib>Berish, Sally A.</creatorcontrib><creatorcontrib>Chen, Cheng‐Yen</creatorcontrib><creatorcontrib>Morse, Stephen</creatorcontrib><creatorcontrib>Trees, David L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Genco, Caroline Attardo</au><au>Berish, Sally A.</au><au>Chen, Cheng‐Yen</au><au>Morse, Stephen</au><au>Trees, David L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity of the iron‐binding protein (Fbp) gene of the pathogenic and commensal Neisseria</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1994-02-15</date><risdate>1994</risdate><volume>116</volume><issue>2</issue><spage>123</spage><epage>129</epage><pages>123-129</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>The pathogenic Neisseria and most commensal Neisseria species produce an iron‐binding protein (Fbp) when grown under iron‐limited conditions. In the current study, we confirmed the presence of Fbp, as well as DNA sequences homologous to the gonococcal fbp, in strains of N. gonorrhoeae, N. meningitidis, N. cinerea, N. lactamica, N. subflava, N. kochii and N. polysaccharea. The fbp genes from these strains were amplified by the polymerase chain reaction, digested with StuI or RsaI, and the restriction patterns examined. The patterns for the gonococcal and meningococcal fbp were virtually identical; however, variations were observed in the fbp sequences of the commensal Neisseria species. N. flavescens, N. mucosa, N. sicca, N. ovis and Branhamella catarrhalis, did not produce Fbp as detected by sodium dodecyl sulfate‐polyacrylamide gel electropheris and reactivity with an Fbp specific monoclonal antibody, nor did they hybridize to an fbp‐specific DNA probe.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8150256</pmid><doi>10.1111/j.1574-6968.1994.tb06690.x</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1994-02, Vol.116 (2), p.123-129 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76426884 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection; Oxford University Press Journals Digital Archive Legacy |
| subjects | Amino Acid Sequence Antibodies, Bacterial Antibodies, Monoclonal Bacterial Outer Membrane Proteins Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - immunology Bacteriology Base Sequence Biological and medical sciences Blotting, Southern DNA Probes DNA, Bacterial - analysis Fbp Fundamental and applied biological sciences. Psychology Genes, Bacterial - genetics Genetic Variation - genetics Genetics Iron acquisition Iron-Binding Proteins Microbiology Molecular Sequence Data Neisseria Neisseria - genetics Neisseria gonorrhoeae Neisseria gonorrhoeae - genetics Neisseria meningitides Neisseria meningitidis - genetics Periplasmic Binding Proteins Polymerase Chain Reaction Restriction Mapping Sequence Analysis Species Specificity |
| title | Genetic diversity of the iron‐binding protein (Fbp) gene of the pathogenic and commensal Neisseria |
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