Nicotine Downregulates α 2 Isoform of Na,K-ATPase at the Blood-Brain Barrier and Brain in Rats
Effects of chronic nicotine treatment on the relative levels of expression of Na,K-ATPase α and β subunit isoforms were determined in rat cerebral microvessels and capillary-depleted brain homogenates by Western blot analysis. Nicotine, 4.5 mg/kg/day, was administered s.c. by osmotic mini-pumps for...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1994-03, Vol.199 (3), p.1422-1427 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Wang, L. Mccomb, J.G. Weiss, M.H. Mcdonough, A.A. Zlokovic, B.V. |
| description | Effects of chronic nicotine treatment on the relative levels of expression of Na,K-ATPase α and β subunit isoforms were determined in rat cerebral microvessels and capillary-depleted brain homogenates by Western blot analysis. Nicotine, 4.5 mg/kg/day, was administered s.c. by osmotic mini-pumps for 14 days. Expression of α
2 isoform in microvessels and brains of nicotine-treated rats was reduced to 0.26 and 0.58 of control levels, respectively. Expression of α
1, α
3, β
1 or β
2 was unchanged. Nicotine decreased cerebromicrovascular and brain Na,ATPase enzymatic activities by 22% and 17%, respectively. It is suggested that chronic exposure to nicotine reduces expression of functional Na,K-ATPase at the blood-brain barrier and brain by downregulating the α
2 isoform. |
| doi_str_mv | 10.1006/bbrc.1994.1389 |
| format | Article |
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2 isoform in microvessels and brains of nicotine-treated rats was reduced to 0.26 and 0.58 of control levels, respectively. Expression of α
1, α
3, β
1 or β
2 was unchanged. Nicotine decreased cerebromicrovascular and brain Na,ATPase enzymatic activities by 22% and 17%, respectively. It is suggested that chronic exposure to nicotine reduces expression of functional Na,K-ATPase at the blood-brain barrier and brain by downregulating the α
2 isoform.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1994.1389</identifier><identifier>PMID: 8147886</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Arterioles - drug effects ; Arterioles - enzymology ; Blood-Brain Barrier - drug effects ; Blood-Brain Barrier - physiology ; Brain - drug effects ; Brain - enzymology ; Capillaries - drug effects ; Capillaries - enzymology ; Cerebrovascular Circulation ; Female ; Gene Expression ; Isoenzymes - biosynthesis ; Isoenzymes - isolation & purification ; Isoenzymes - metabolism ; Male ; Microcirculation - drug effects ; Microcirculation - enzymology ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - enzymology ; Nicotine - pharmacology ; Rats ; Rats, Sprague-Dawley ; Sodium-Potassium-Exchanging ATPase - biosynthesis ; Sodium-Potassium-Exchanging ATPase - isolation & purification ; Sodium-Potassium-Exchanging ATPase - metabolism</subject><ispartof>Biochemical and biophysical research communications, 1994-03, Vol.199 (3), p.1422-1427</ispartof><rights>1994 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X84713891$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8147886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, L.</creatorcontrib><creatorcontrib>Mccomb, J.G.</creatorcontrib><creatorcontrib>Weiss, M.H.</creatorcontrib><creatorcontrib>Mcdonough, A.A.</creatorcontrib><creatorcontrib>Zlokovic, B.V.</creatorcontrib><title>Nicotine Downregulates α 2 Isoform of Na,K-ATPase at the Blood-Brain Barrier and Brain in Rats</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Effects of chronic nicotine treatment on the relative levels of expression of Na,K-ATPase α and β subunit isoforms were determined in rat cerebral microvessels and capillary-depleted brain homogenates by Western blot analysis. Nicotine, 4.5 mg/kg/day, was administered s.c. by osmotic mini-pumps for 14 days. Expression of α
2 isoform in microvessels and brains of nicotine-treated rats was reduced to 0.26 and 0.58 of control levels, respectively. Expression of α
1, α
3, β
1 or β
2 was unchanged. Nicotine decreased cerebromicrovascular and brain Na,ATPase enzymatic activities by 22% and 17%, respectively. It is suggested that chronic exposure to nicotine reduces expression of functional Na,K-ATPase at the blood-brain barrier and brain by downregulating the α
2 isoform.</description><subject>Animals</subject><subject>Arterioles - drug effects</subject><subject>Arterioles - enzymology</subject><subject>Blood-Brain Barrier - drug effects</subject><subject>Blood-Brain Barrier - physiology</subject><subject>Brain - drug effects</subject><subject>Brain - enzymology</subject><subject>Capillaries - drug effects</subject><subject>Capillaries - enzymology</subject><subject>Cerebrovascular Circulation</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Isoenzymes - biosynthesis</subject><subject>Isoenzymes - isolation & purification</subject><subject>Isoenzymes - metabolism</subject><subject>Male</subject><subject>Microcirculation - drug effects</subject><subject>Microcirculation - enzymology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - enzymology</subject><subject>Nicotine - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sodium-Potassium-Exchanging ATPase - biosynthesis</subject><subject>Sodium-Potassium-Exchanging ATPase - isolation & purification</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkM9OwzAMxiMEGmNw5YaUEyc6krRLmuM2_k1MA6EhcYuS1oWgroGkBfFYvAjPRKrNsmTZ3yfL_iF0SsmYEsIvjfHFmEqZjWmayz00pESShFGS7aMhiY6ESfpyiI5CeCeE0ozLARrkNBN5zodIrWzhWtsAvnLfjYfXrtYtBPz3ixleBFc5v8Guwit9cZ9M1486ANYtbt8Az2rnymTmtW3wTHtvwWPdlHg7ifmk23CMDipdBzjZ1RF6vrlez--S5cPtYj5dJkAJ44koCdETJkxV0cpwkYuUC6Apkyb2UsfgWlR8UglGaGH0RMoSDAiRMjCZSEfofLv3w7vPDkKrNjYUUNe6AdcFJXjGOOM8Gs92xs5soFQf3m60_1E7IlHPtzrEa7_iTyoUFpoCSuuhaFXprKJE9exVz1717FXPPv0HM9l1Xw</recordid><startdate>19940330</startdate><enddate>19940330</enddate><creator>Wang, L.</creator><creator>Mccomb, J.G.</creator><creator>Weiss, M.H.</creator><creator>Mcdonough, A.A.</creator><creator>Zlokovic, B.V.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19940330</creationdate><title>Nicotine Downregulates α 2 Isoform of Na,K-ATPase at the Blood-Brain Barrier and Brain in Rats</title><author>Wang, L. ; Mccomb, J.G. ; Weiss, M.H. ; Mcdonough, A.A. ; Zlokovic, B.V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1026-7d00a527bff1fb6787367e1329b1fb9aaaa6a7f65f7201cba599debe7732eb473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Arterioles - drug effects</topic><topic>Arterioles - enzymology</topic><topic>Blood-Brain Barrier - drug effects</topic><topic>Blood-Brain Barrier - physiology</topic><topic>Brain - drug effects</topic><topic>Brain - enzymology</topic><topic>Capillaries - drug effects</topic><topic>Capillaries - enzymology</topic><topic>Cerebrovascular Circulation</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Isoenzymes - biosynthesis</topic><topic>Isoenzymes - isolation & purification</topic><topic>Isoenzymes - metabolism</topic><topic>Male</topic><topic>Microcirculation - drug effects</topic><topic>Microcirculation - enzymology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - enzymology</topic><topic>Nicotine - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sodium-Potassium-Exchanging ATPase - biosynthesis</topic><topic>Sodium-Potassium-Exchanging ATPase - isolation & purification</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, L.</creatorcontrib><creatorcontrib>Mccomb, J.G.</creatorcontrib><creatorcontrib>Weiss, M.H.</creatorcontrib><creatorcontrib>Mcdonough, A.A.</creatorcontrib><creatorcontrib>Zlokovic, B.V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, L.</au><au>Mccomb, J.G.</au><au>Weiss, M.H.</au><au>Mcdonough, A.A.</au><au>Zlokovic, B.V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nicotine Downregulates α 2 Isoform of Na,K-ATPase at the Blood-Brain Barrier and Brain in Rats</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-03-30</date><risdate>1994</risdate><volume>199</volume><issue>3</issue><spage>1422</spage><epage>1427</epage><pages>1422-1427</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Effects of chronic nicotine treatment on the relative levels of expression of Na,K-ATPase α and β subunit isoforms were determined in rat cerebral microvessels and capillary-depleted brain homogenates by Western blot analysis. Nicotine, 4.5 mg/kg/day, was administered s.c. by osmotic mini-pumps for 14 days. Expression of α
2 isoform in microvessels and brains of nicotine-treated rats was reduced to 0.26 and 0.58 of control levels, respectively. Expression of α
1, α
3, β
1 or β
2 was unchanged. Nicotine decreased cerebromicrovascular and brain Na,ATPase enzymatic activities by 22% and 17%, respectively. It is suggested that chronic exposure to nicotine reduces expression of functional Na,K-ATPase at the blood-brain barrier and brain by downregulating the α
2 isoform.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8147886</pmid><doi>10.1006/bbrc.1994.1389</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1994-03, Vol.199 (3), p.1422-1427 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Arterioles - drug effects Arterioles - enzymology Blood-Brain Barrier - drug effects Blood-Brain Barrier - physiology Brain - drug effects Brain - enzymology Capillaries - drug effects Capillaries - enzymology Cerebrovascular Circulation Female Gene Expression Isoenzymes - biosynthesis Isoenzymes - isolation & purification Isoenzymes - metabolism Male Microcirculation - drug effects Microcirculation - enzymology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - enzymology Nicotine - pharmacology Rats Rats, Sprague-Dawley Sodium-Potassium-Exchanging ATPase - biosynthesis Sodium-Potassium-Exchanging ATPase - isolation & purification Sodium-Potassium-Exchanging ATPase - metabolism |
| title | Nicotine Downregulates α 2 Isoform of Na,K-ATPase at the Blood-Brain Barrier and Brain in Rats |
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