Formylmethanofuran Synthesis by Formylmethanofuran Dehydrogenase from Methanobacterium thermoautotrophicum Marburg
Formylmethanofuran dehydrogenase was purified 30-fold from the cytosolic fraction of cell extract of Methanobacterium thermoautotrophicum (Marburg) and shown for the first time to synthesize in vitro formylmethanofuran from methanofuran and carbon dioxide with electrons donated by titanium(III) citr...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1994-03, Vol.199 (3), p.1256-1261 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Wasserfallen, A. |
| description | Formylmethanofuran dehydrogenase was purified 30-fold from the cytosolic fraction of cell extract of
Methanobacterium thermoautotrophicum (Marburg) and shown for the first time to synthesize
in vitro formylmethanofuran from methanofuran and carbon dioxide with electrons donated by titanium(III) citrate. The reaction was methanofuran-, CO
2-, and Ti
3+-dependent. Active enzyme could be purified from cells grown with either molybdenum or tungsten as the sole group VIA trace element. The active form of formylmethanofuran dehydrogenase had an apparent molecular mass of 530 kDa as determined by gel filtration chromatography and was found to copurify with a hydrogenase. |
| doi_str_mv | 10.1006/bbrc.1994.1366 |
| format | Article |
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Methanobacterium thermoautotrophicum (Marburg) and shown for the first time to synthesize
in vitro formylmethanofuran from methanofuran and carbon dioxide with electrons donated by titanium(III) citrate. The reaction was methanofuran-, CO
2-, and Ti
3+-dependent. Active enzyme could be purified from cells grown with either molybdenum or tungsten as the sole group VIA trace element. The active form of formylmethanofuran dehydrogenase had an apparent molecular mass of 530 kDa as determined by gel filtration chromatography and was found to copurify with a hydrogenase.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1994.1366</identifier><identifier>PMID: 8147868</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aldehyde Oxidoreductases - isolation & purification ; Aldehyde Oxidoreductases - metabolism ; Carbon Dioxide - pharmacology ; Chromatography ; Chromatography, Gel ; Chromatography, Ion Exchange ; Durapatite ; Electrophoresis, Polyacrylamide Gel ; Furans - metabolism ; Kinetics ; Methanobacterium - enzymology ; Methanobacterium thermoautotrophicum ; Molecular Weight ; Titanium - pharmacology</subject><ispartof>Biochemical and biophysical research communications, 1994-03, Vol.199 (3), p.1256-1261</ispartof><rights>1994 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-7209d2d5d719be13c18742966bde51864e49bbec0dd843588886a81aaabeb5ee3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X84713660$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8147868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wasserfallen, A.</creatorcontrib><title>Formylmethanofuran Synthesis by Formylmethanofuran Dehydrogenase from Methanobacterium thermoautotrophicum Marburg</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Formylmethanofuran dehydrogenase was purified 30-fold from the cytosolic fraction of cell extract of
Methanobacterium thermoautotrophicum (Marburg) and shown for the first time to synthesize
in vitro formylmethanofuran from methanofuran and carbon dioxide with electrons donated by titanium(III) citrate. The reaction was methanofuran-, CO
2-, and Ti
3+-dependent. Active enzyme could be purified from cells grown with either molybdenum or tungsten as the sole group VIA trace element. The active form of formylmethanofuran dehydrogenase had an apparent molecular mass of 530 kDa as determined by gel filtration chromatography and was found to copurify with a hydrogenase.</description><subject>Aldehyde Oxidoreductases - isolation & purification</subject><subject>Aldehyde Oxidoreductases - metabolism</subject><subject>Carbon Dioxide - pharmacology</subject><subject>Chromatography</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Ion Exchange</subject><subject>Durapatite</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Furans - metabolism</subject><subject>Kinetics</subject><subject>Methanobacterium - enzymology</subject><subject>Methanobacterium thermoautotrophicum</subject><subject>Molecular Weight</subject><subject>Titanium - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EapeWK7dKOXHL4kkcxz5WhQJSKw6A1Jvlj9mu0SbejhOk_Hu82lVPRfgykt9nnsO8jL0HvgbO5UfnyK9Ba7GGVspXbAVc87oBLl6zFS9E3Wh4OGdvc_7NOYCQ-oydKRC9kmrF6DbRsOwGnLZ2TJuZ7Fj9WMZpiznmyi3VC_kn3C6B0iOONmO1oTRU98fcWT8hxXmoioCGZOcpTZT22-jL370lN9PjJXuzsbuM707zgv26_fzz5mt99_3Lt5vru9q3PZ_qvuE6NKELPWiH0HpQvWi0lC5gB0oKFNo59DwEJdpOlSetAmutQ9chthfsw9G7p_Q0Y57MELPH3c6OmOZseikaCZ38LwiyqLu2K-D6CHpKORNuzJ7iYGkxwM2hDXNowxzaMIc2ysLVyTy7AcMzfjp_ydUxx3KHPxHJZB9x9BgioZ9MSPFf6r_c8Z0v</recordid><startdate>19940330</startdate><enddate>19940330</enddate><creator>Wasserfallen, A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19940330</creationdate><title>Formylmethanofuran Synthesis by Formylmethanofuran Dehydrogenase from Methanobacterium thermoautotrophicum Marburg</title><author>Wasserfallen, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-7209d2d5d719be13c18742966bde51864e49bbec0dd843588886a81aaabeb5ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Aldehyde Oxidoreductases - isolation & purification</topic><topic>Aldehyde Oxidoreductases - metabolism</topic><topic>Carbon Dioxide - pharmacology</topic><topic>Chromatography</topic><topic>Chromatography, Gel</topic><topic>Chromatography, Ion Exchange</topic><topic>Durapatite</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Furans - metabolism</topic><topic>Kinetics</topic><topic>Methanobacterium - enzymology</topic><topic>Methanobacterium thermoautotrophicum</topic><topic>Molecular Weight</topic><topic>Titanium - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wasserfallen, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wasserfallen, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Formylmethanofuran Synthesis by Formylmethanofuran Dehydrogenase from Methanobacterium thermoautotrophicum Marburg</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-03-30</date><risdate>1994</risdate><volume>199</volume><issue>3</issue><spage>1256</spage><epage>1261</epage><pages>1256-1261</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Formylmethanofuran dehydrogenase was purified 30-fold from the cytosolic fraction of cell extract of
Methanobacterium thermoautotrophicum (Marburg) and shown for the first time to synthesize
in vitro formylmethanofuran from methanofuran and carbon dioxide with electrons donated by titanium(III) citrate. The reaction was methanofuran-, CO
2-, and Ti
3+-dependent. Active enzyme could be purified from cells grown with either molybdenum or tungsten as the sole group VIA trace element. The active form of formylmethanofuran dehydrogenase had an apparent molecular mass of 530 kDa as determined by gel filtration chromatography and was found to copurify with a hydrogenase.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8147868</pmid><doi>10.1006/bbrc.1994.1366</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1994-03, Vol.199 (3), p.1256-1261 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76426156 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aldehyde Oxidoreductases - isolation & purification Aldehyde Oxidoreductases - metabolism Carbon Dioxide - pharmacology Chromatography Chromatography, Gel Chromatography, Ion Exchange Durapatite Electrophoresis, Polyacrylamide Gel Furans - metabolism Kinetics Methanobacterium - enzymology Methanobacterium thermoautotrophicum Molecular Weight Titanium - pharmacology |
| title | Formylmethanofuran Synthesis by Formylmethanofuran Dehydrogenase from Methanobacterium thermoautotrophicum Marburg |
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