Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5
To better understand the regulation of insulin-like growth factor binding protein-5 (IGFBP-5) expression, we cloned the IGFBP-5 gene from human genomic libraries and identified a region in the 5' flanking sequence which functions as a promoter. The human IGFBP-5 gene is divided into four exons...
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| Veröffentlicht in: | The Journal of biological chemistry 1994-04, Vol.269 (14), p.10891-10898 |
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| container_title | The Journal of biological chemistry |
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| creator | ALLANDER, S. V LARSSON, C EHRENBORG, E SUWANICHKUL, A WEBER, G MORRIS, S. L BAJALICA, S KIEFER, M. C LUTHMAN, H POWELL, D. R |
| description | To better understand the regulation of insulin-like growth factor binding protein-5 (IGFBP-5) expression, we cloned the IGFBP-5
gene from human genomic libraries and identified a region in the 5' flanking sequence which functions as a promoter. The human
IGFBP-5 gene is divided into four exons which, primarily due to a first intron of approximately 25 kilobases, span approximately
33 kilobases of DNA. Southern analysis identified a single copy of the IGFBP-5 gene in the haploid human genome, and several
independent mapping strategies found this gene tightly linked with, and in opposite transcriptional orientation to, the IGFBP-2
gene at chromosomal region 2q33-34. Primer extension studies identified the IGFBP-5 mRNA cap site 772 base pairs (bp) 5' to
the first nucleotide of the translation start codon. Analysis of the 5'-flanking sequence identified a potential TATA element
beginning 33 bp 5' to the mRNA cap site. When a DNA fragment containing this cap site and 461 bp of upstream sequence was
placed 5' to the chloramphenicol acetyltransferase reporter gene and transfected into MDA-MB-468 human breast cancer cells,
it directed chloramphenicol acetyltransferase expression in an orientation-specific manner, suggesting that this region contains
elements essential for IGFBP-5 promoter activity. |
| doi_str_mv | 10.1016/S0021-9258(17)34142-X |
| format | Article |
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gene from human genomic libraries and identified a region in the 5' flanking sequence which functions as a promoter. The human
IGFBP-5 gene is divided into four exons which, primarily due to a first intron of approximately 25 kilobases, span approximately
33 kilobases of DNA. Southern analysis identified a single copy of the IGFBP-5 gene in the haploid human genome, and several
independent mapping strategies found this gene tightly linked with, and in opposite transcriptional orientation to, the IGFBP-2
gene at chromosomal region 2q33-34. Primer extension studies identified the IGFBP-5 mRNA cap site 772 base pairs (bp) 5' to
the first nucleotide of the translation start codon. Analysis of the 5'-flanking sequence identified a potential TATA element
beginning 33 bp 5' to the mRNA cap site. When a DNA fragment containing this cap site and 461 bp of upstream sequence was
placed 5' to the chloramphenicol acetyltransferase reporter gene and transfected into MDA-MB-468 human breast cancer cells,
it directed chloramphenicol acetyltransferase expression in an orientation-specific manner, suggesting that this region contains
elements essential for IGFBP-5 promoter activity.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)34142-X</identifier><identifier>PMID: 7511611</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cells, Cultured ; Chromosome Mapping ; Chromosomes, Human ; DNA, Complementary ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Humans ; Insulin-Like Growth Factor Binding Protein 5 ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Promoter Regions, Genetic ; RNA, Messenger - metabolism ; Somatomedins - metabolism</subject><ispartof>The Journal of biological chemistry, 1994-04, Vol.269 (14), p.10891-10898</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-2acdc7f928f85fae679ec29fde0fba65a264e2a58744b02c2902d5bc638587233</citedby><cites>FETCH-LOGICAL-c461t-2acdc7f928f85fae679ec29fde0fba65a264e2a58744b02c2902d5bc638587233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4134003$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7511611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ALLANDER, S. V</creatorcontrib><creatorcontrib>LARSSON, C</creatorcontrib><creatorcontrib>EHRENBORG, E</creatorcontrib><creatorcontrib>SUWANICHKUL, A</creatorcontrib><creatorcontrib>WEBER, G</creatorcontrib><creatorcontrib>MORRIS, S. L</creatorcontrib><creatorcontrib>BAJALICA, S</creatorcontrib><creatorcontrib>KIEFER, M. C</creatorcontrib><creatorcontrib>LUTHMAN, H</creatorcontrib><creatorcontrib>POWELL, D. R</creatorcontrib><title>Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>To better understand the regulation of insulin-like growth factor binding protein-5 (IGFBP-5) expression, we cloned the IGFBP-5
gene from human genomic libraries and identified a region in the 5' flanking sequence which functions as a promoter. The human
IGFBP-5 gene is divided into four exons which, primarily due to a first intron of approximately 25 kilobases, span approximately
33 kilobases of DNA. Southern analysis identified a single copy of the IGFBP-5 gene in the haploid human genome, and several
independent mapping strategies found this gene tightly linked with, and in opposite transcriptional orientation to, the IGFBP-2
gene at chromosomal region 2q33-34. Primer extension studies identified the IGFBP-5 mRNA cap site 772 base pairs (bp) 5' to
the first nucleotide of the translation start codon. Analysis of the 5'-flanking sequence identified a potential TATA element
beginning 33 bp 5' to the mRNA cap site. When a DNA fragment containing this cap site and 461 bp of upstream sequence was
placed 5' to the chloramphenicol acetyltransferase reporter gene and transfected into MDA-MB-468 human breast cancer cells,
it directed chloramphenicol acetyltransferase expression in an orientation-specific manner, suggesting that this region contains
elements essential for IGFBP-5 promoter activity.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human</subject><subject>DNA, Complementary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Humans</subject><subject>Insulin-Like Growth Factor Binding Protein 5</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Promoter Regions, Genetic</subject><subject>RNA, Messenger - metabolism</subject><subject>Somatomedins - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE9r3DAQxUVpSDdpP0JAh1Kag1ONLMn2sSz9B4Ee2sLehCyP1mptKZVsQvLpq02WrS6Ceb83b3iEXAG7AQbqww_GOFQdl-17aK5rAYJXuxdkA6ytq1rC7iXZnJBX5CLn36w80cE5OW8kgALYkGk7mmTsgsk_msXHQKOjy4jUjinOMcfZTHSPAakJA707zApLXUx0XGcTqA95nXyoJv8H6T7F-2WkriwsQO_D4MP-4FqwIPI1OXNmyvjm-F-SX58__dx-rW6_f_m2_XhbWaFgqbixg21cx1vXSmdQNR1a3rkBmeuNkoYrgdzIthGiZ7xIjA-yt6puy4zX9SV597y3JP9dMS969tniNJmAcc26UYILqFkB5TNoU8w5odN3yc8mPWhg-tCyfmpZHyrU0OinlvWu-K6OAWs_43ByHWst-tujbrI1k0smWJ9PWMkWjNX_sdHvx3ufUPc-2hFnzVWnQZQT2g7qf2L9klw</recordid><startdate>19940408</startdate><enddate>19940408</enddate><creator>ALLANDER, S. V</creator><creator>LARSSON, C</creator><creator>EHRENBORG, E</creator><creator>SUWANICHKUL, A</creator><creator>WEBER, G</creator><creator>MORRIS, S. L</creator><creator>BAJALICA, S</creator><creator>KIEFER, M. C</creator><creator>LUTHMAN, H</creator><creator>POWELL, D. R</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940408</creationdate><title>Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5</title><author>ALLANDER, S. V ; LARSSON, C ; EHRENBORG, E ; SUWANICHKUL, A ; WEBER, G ; MORRIS, S. L ; BAJALICA, S ; KIEFER, M. C ; LUTHMAN, H ; POWELL, D. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-2acdc7f928f85fae679ec29fde0fba65a264e2a58744b02c2902d5bc638587233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cells, Cultured</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human</topic><topic>DNA, Complementary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Humans</topic><topic>Insulin-Like Growth Factor Binding Protein 5</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Promoter Regions, Genetic</topic><topic>RNA, Messenger - metabolism</topic><topic>Somatomedins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ALLANDER, S. V</creatorcontrib><creatorcontrib>LARSSON, C</creatorcontrib><creatorcontrib>EHRENBORG, E</creatorcontrib><creatorcontrib>SUWANICHKUL, A</creatorcontrib><creatorcontrib>WEBER, G</creatorcontrib><creatorcontrib>MORRIS, S. L</creatorcontrib><creatorcontrib>BAJALICA, S</creatorcontrib><creatorcontrib>KIEFER, M. C</creatorcontrib><creatorcontrib>LUTHMAN, H</creatorcontrib><creatorcontrib>POWELL, D. 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R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-04-08</date><risdate>1994</risdate><volume>269</volume><issue>14</issue><spage>10891</spage><epage>10898</epage><pages>10891-10898</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>To better understand the regulation of insulin-like growth factor binding protein-5 (IGFBP-5) expression, we cloned the IGFBP-5
gene from human genomic libraries and identified a region in the 5' flanking sequence which functions as a promoter. The human
IGFBP-5 gene is divided into four exons which, primarily due to a first intron of approximately 25 kilobases, span approximately
33 kilobases of DNA. Southern analysis identified a single copy of the IGFBP-5 gene in the haploid human genome, and several
independent mapping strategies found this gene tightly linked with, and in opposite transcriptional orientation to, the IGFBP-2
gene at chromosomal region 2q33-34. Primer extension studies identified the IGFBP-5 mRNA cap site 772 base pairs (bp) 5' to
the first nucleotide of the translation start codon. Analysis of the 5'-flanking sequence identified a potential TATA element
beginning 33 bp 5' to the mRNA cap site. When a DNA fragment containing this cap site and 461 bp of upstream sequence was
placed 5' to the chloramphenicol acetyltransferase reporter gene and transfected into MDA-MB-468 human breast cancer cells,
it directed chloramphenicol acetyltransferase expression in an orientation-specific manner, suggesting that this region contains
elements essential for IGFBP-5 promoter activity.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>7511611</pmid><doi>10.1016/S0021-9258(17)34142-X</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1994-04, Vol.269 (14), p.10891-10898 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76424130 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - metabolism Cells, Cultured Chromosome Mapping Chromosomes, Human DNA, Complementary Fundamental and applied biological sciences. Psychology Genes. Genome Humans Insulin-Like Growth Factor Binding Protein 5 Molecular and cellular biology Molecular genetics Molecular Sequence Data Promoter Regions, Genetic RNA, Messenger - metabolism Somatomedins - metabolism |
| title | Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5 |
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