Intramolecular charge heterogeneity in purified major histocompatibility class II alpha and beta polypeptide chains

Major histocompatibility (MHC) class II antigens are heterodimeric cell surface glycoproteins consisting of an alpha and a beta chain. Although one-dimensional SDS-polyacrylamide gel electrophoresis analysis of purified MHC class II antigens shows a single diffuse band for each chain, multiple spots...

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Veröffentlicht in:	The Journal of biological chemistry 1994-04, Vol.269 (13), p.10061-10070
Hauptverfasser:	NAG, B, ARIMILLI, S, KOUKIS, B, RHODES, E, BAICHWAL, V, SHARMA, S. D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Antigens Biological and medical sciences Blotting, Western Cell Line Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Fundamental immunology Glycosylation Histocompatibility antigens (hla, h-2 and other systems) Histocompatibility Antigens - chemistry Histocompatibility Antigens - isolation & purification Histocompatibility Antigens Class II - chemistry Histocompatibility Antigens Class II - isolation & purification HLA-DR Antigens - chemistry HLA-DR Antigens - isolation & purification HLA-DR2 Antigen - chemistry HLA-DR3 Antigen - chemistry HLA-DR4 Antigen - chemistry Humans Hybridomas Isoelectric Focusing Macromolecular Substances Molecular immunology Protein Conformation Rats Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification
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