Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region
Latently infected B lymphocytes continuously express an Epstein-Barr Virus nuclear antigen (EBNA-1) required in trans for maintenance of the plasmid state of the EBV genome. Filter binding assays and DNAase I footprinting analyses revealed that the carboxy-terminal domain of EBNA-1 protects binding...
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| Veröffentlicht in: | Cell 1985-10, Vol.42 (3), p.859-868 |
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| creator | Rawlins, Dan R. Milman, Gregory Hayward, S.Diane Hayward, Gary S. |
| description | Latently infected B lymphocytes continuously express an Epstein-Barr Virus nuclear antigen (EBNA-1) required
in trans for maintenance of the plasmid state of the EBV genome. Filter binding assays and DNAase I footprinting analyses revealed that the carboxy-terminal domain of EBNA-1 protects binding sites at three different loci in the 172,000 bp EBV genome. Two of these loci correspond to essential elements within an 1800 bp segment defined as the minimal region required for plasmid maintenance (
ori-P). Binding to each of 20 × 30 bp tandem repeats in the “sink” locus protects 25 bp centered over a 12 bp palindromic consensus sequence TAGCATATGCTA. The nearby dyad symmetry “origin” locus contains two 46 bp protected regions each encompassing two paired core binding sites. The demonstration of sequence-specific binding at multiple loci suggests that EBNA-1 has pleiotropic functions, which may include control of copy number and segregation of the EBV plasmids as well as initiation of replication. |
| doi_str_mv | 10.1016/0092-8674(85)90282-X |
| format | Article |
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in trans for maintenance of the plasmid state of the EBV genome. Filter binding assays and DNAase I footprinting analyses revealed that the carboxy-terminal domain of EBNA-1 protects binding sites at three different loci in the 172,000 bp EBV genome. Two of these loci correspond to essential elements within an 1800 bp segment defined as the minimal region required for plasmid maintenance (
ori-P). Binding to each of 20 × 30 bp tandem repeats in the “sink” locus protects 25 bp centered over a 12 bp palindromic consensus sequence TAGCATATGCTA. The nearby dyad symmetry “origin” locus contains two 46 bp protected regions each encompassing two paired core binding sites. The demonstration of sequence-specific binding at multiple loci suggests that EBNA-1 has pleiotropic functions, which may include control of copy number and segregation of the EBV plasmids as well as initiation of replication.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(85)90282-X</identifier><identifier>PMID: 2996781</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Antigens, Viral - genetics ; B-Lymphocytes - immunology ; Base Sequence ; Biological and medical sciences ; Cell Line ; Cloning, Molecular ; DNA Restriction Enzymes ; DNA, Viral - genetics ; Epstein-Barr virus ; Epstein-Barr Virus Nuclear Antigens ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genes, Viral ; Herpesvirus 4, Human - genetics ; Herpesvirus 4, Human - immunology ; Humans ; Molecular and cellular biology ; Molecular genetics ; Plasmids ; Sequence Homology, Nucleic Acid</subject><ispartof>Cell, 1985-10, Vol.42 (3), p.859-868</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-f4c4739fd95140c52203a185bedab6d9de915224ff827c5c53b6aa5556a5018c3</citedby><cites>FETCH-LOGICAL-c483t-f4c4739fd95140c52203a185bedab6d9de915224ff827c5c53b6aa5556a5018c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0092-8674(85)90282-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8753551$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2996781$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rawlins, Dan R.</creatorcontrib><creatorcontrib>Milman, Gregory</creatorcontrib><creatorcontrib>Hayward, S.Diane</creatorcontrib><creatorcontrib>Hayward, Gary S.</creatorcontrib><title>Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region</title><title>Cell</title><addtitle>Cell</addtitle><description>Latently infected B lymphocytes continuously express an Epstein-Barr Virus nuclear antigen (EBNA-1) required
in trans for maintenance of the plasmid state of the EBV genome. Filter binding assays and DNAase I footprinting analyses revealed that the carboxy-terminal domain of EBNA-1 protects binding sites at three different loci in the 172,000 bp EBV genome. Two of these loci correspond to essential elements within an 1800 bp segment defined as the minimal region required for plasmid maintenance (
ori-P). Binding to each of 20 × 30 bp tandem repeats in the “sink” locus protects 25 bp centered over a 12 bp palindromic consensus sequence TAGCATATGCTA. The nearby dyad symmetry “origin” locus contains two 46 bp protected regions each encompassing two paired core binding sites. The demonstration of sequence-specific binding at multiple loci suggests that EBNA-1 has pleiotropic functions, which may include control of copy number and segregation of the EBV plasmids as well as initiation of replication.</description><subject>Antigens, Viral - genetics</subject><subject>B-Lymphocytes - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Viral - genetics</subject><subject>Epstein-Barr virus</subject><subject>Epstein-Barr Virus Nuclear Antigens</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Genes, Viral</subject><subject>Herpesvirus 4, Human - genetics</subject><subject>Herpesvirus 4, Human - immunology</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Plasmids</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQhy0EKkvhDUDyAaH2ELAdO3EuSNt2-SNV5QBIvVmOPVkGZZ3UTirxALx3ne5qj3Cy5PnmZ898hLzm7D1nvPrAWCMKXdXyTKvzhgktitsnZMVZUxeS1-IpWR2R5-RFSr8ZY1opdUJORNNUteYr8vc73M0QHBRpBIcdOnp1s6YtBo9hS4eOTr-AbsY0AYbiwsZI7zHOiYbZ9WAjtWHCLQR6trm4WRf8nE4Ddf2c-QieJpwgUQyPKWNv0w493VkMEwSbX6URtjiEl-RZZ_sErw7nKfn5afPj8ktx_e3z18v1deGkLqeik07WZdP5RnHJnBKClZZr1YK3beUbDw3Pl7LrtKidcqpsK2vzxJVVjGtXnpJ3-9wxDnnsNJkdJgd9bwMMczJ1JQUTqvovyKVkqtR1BuUedHFIKUJnxog7G_8YzsyiySwOzOLAaGUeNZnb3PbmkD-3O_DHpoOXXH97qNvkbN_FvCxMR0zXqlRqwT7uMchLu0eIJjlcbHqM4CbjB_z3Px4AUzWuXg</recordid><startdate>198510</startdate><enddate>198510</enddate><creator>Rawlins, Dan R.</creator><creator>Milman, Gregory</creator><creator>Hayward, S.Diane</creator><creator>Hayward, Gary S.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198510</creationdate><title>Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region</title><author>Rawlins, Dan R. ; Milman, Gregory ; Hayward, S.Diane ; Hayward, Gary S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-f4c4739fd95140c52203a185bedab6d9de915224ff827c5c53b6aa5556a5018c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Antigens, Viral - genetics</topic><topic>B-Lymphocytes - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cloning, Molecular</topic><topic>DNA Restriction Enzymes</topic><topic>DNA, Viral - genetics</topic><topic>Epstein-Barr virus</topic><topic>Epstein-Barr Virus Nuclear Antigens</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Genes, Viral</topic><topic>Herpesvirus 4, Human - genetics</topic><topic>Herpesvirus 4, Human - immunology</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Plasmids</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rawlins, Dan R.</creatorcontrib><creatorcontrib>Milman, Gregory</creatorcontrib><creatorcontrib>Hayward, S.Diane</creatorcontrib><creatorcontrib>Hayward, Gary S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rawlins, Dan R.</au><au>Milman, Gregory</au><au>Hayward, S.Diane</au><au>Hayward, Gary S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1985-10</date><risdate>1985</risdate><volume>42</volume><issue>3</issue><spage>859</spage><epage>868</epage><pages>859-868</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>Latently infected B lymphocytes continuously express an Epstein-Barr Virus nuclear antigen (EBNA-1) required
in trans for maintenance of the plasmid state of the EBV genome. Filter binding assays and DNAase I footprinting analyses revealed that the carboxy-terminal domain of EBNA-1 protects binding sites at three different loci in the 172,000 bp EBV genome. Two of these loci correspond to essential elements within an 1800 bp segment defined as the minimal region required for plasmid maintenance (
ori-P). Binding to each of 20 × 30 bp tandem repeats in the “sink” locus protects 25 bp centered over a 12 bp palindromic consensus sequence TAGCATATGCTA. The nearby dyad symmetry “origin” locus contains two 46 bp protected regions each encompassing two paired core binding sites. The demonstration of sequence-specific binding at multiple loci suggests that EBNA-1 has pleiotropic functions, which may include control of copy number and segregation of the EBV plasmids as well as initiation of replication.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>2996781</pmid><doi>10.1016/0092-8674(85)90282-X</doi><tpages>10</tpages></addata></record> |
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| ispartof | Cell, 1985-10, Vol.42 (3), p.859-868 |
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| subjects | Antigens, Viral - genetics B-Lymphocytes - immunology Base Sequence Biological and medical sciences Cell Line Cloning, Molecular DNA Restriction Enzymes DNA, Viral - genetics Epstein-Barr virus Epstein-Barr Virus Nuclear Antigens Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene expression Genes, Viral Herpesvirus 4, Human - genetics Herpesvirus 4, Human - immunology Humans Molecular and cellular biology Molecular genetics Plasmids Sequence Homology, Nucleic Acid |
| title | Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region |
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