Cisplatin Induces Apoptosis in a Human Ovarian Carcinoma Cell Line without Concomitant Internucleosomal Degradation of DNA
After treatment of the human ovarian carcinoma cell line, CH1, with cisplatin, cells detached from the culture dish in a time- and dose-dependent fashion. These cells showed morphological changes indicative of apoptosis. Their DNA had not been degraded into oligonucleosomal fragments, but the DNA ha...
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| Veröffentlicht in: | Experimental cell research 1994-04, Vol.211 (2), p.231-237 |
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| creator | Ormerod, M.G. O'Neill, C.F. Robertson, D. Harrap, K.R. |
| description | After treatment of the human ovarian carcinoma cell line, CH1, with cisplatin, cells detached from the culture dish in a time- and dose-dependent fashion. These cells showed morphological changes indicative of apoptosis. Their DNA had not been degraded into oligonucleosomal fragments, but the DNA had been cut into larger fragments (30 kbp) of a size associated with chromatin loops. We conclude that cisplatin killed these ovarian cells by inducing apoptosis. However, in these cells, apoptosis was not accompanied by internucleosoreal degradation of DNA. Our data are consistent with the hypothesis that the introduction of a double-strand break at a specific site in the chromatin loops is an early event in apoptosis. This degradation is accompanied by morphologically observable changes in chromatin structure. Internucleosomal degradation, when it occurs, is a late event. |
| doi_str_mv | 10.1006/excr.1994.1082 |
| format | Article |
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These cells showed morphological changes indicative of apoptosis. Their DNA had not been degraded into oligonucleosomal fragments, but the DNA had been cut into larger fragments (30 kbp) of a size associated with chromatin loops. We conclude that cisplatin killed these ovarian cells by inducing apoptosis. However, in these cells, apoptosis was not accompanied by internucleosoreal degradation of DNA. Our data are consistent with the hypothesis that the introduction of a double-strand break at a specific site in the chromatin loops is an early event in apoptosis. This degradation is accompanied by morphologically observable changes in chromatin structure. 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Psychology ; Humans ; Microscopy, Electron ; Molecular and cellular biology ; Molecular Weight ; Nucleosomes - drug effects ; Nucleosomes - metabolism ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; Tumor Cells, Cultured - drug effects ; Tumor Cells, Cultured - metabolism ; Tumor Cells, Cultured - pathology</subject><ispartof>Experimental cell research, 1994-04, Vol.211 (2), p.231-237</ispartof><rights>1994 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-24f49d039c5aa47245a89c09a4d121c3ec43ba3ab0589654613d3b559e634ebc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014482784710822$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4124086$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8143768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ormerod, M.G.</creatorcontrib><creatorcontrib>O'Neill, C.F.</creatorcontrib><creatorcontrib>Robertson, D.</creatorcontrib><creatorcontrib>Harrap, K.R.</creatorcontrib><title>Cisplatin Induces Apoptosis in a Human Ovarian Carcinoma Cell Line without Concomitant Internucleosomal Degradation of DNA</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>After treatment of the human ovarian carcinoma cell line, CH1, with cisplatin, cells detached from the culture dish in a time- and dose-dependent fashion. These cells showed morphological changes indicative of apoptosis. Their DNA had not been degraded into oligonucleosomal fragments, but the DNA had been cut into larger fragments (30 kbp) of a size associated with chromatin loops. We conclude that cisplatin killed these ovarian cells by inducing apoptosis. However, in these cells, apoptosis was not accompanied by internucleosoreal degradation of DNA. Our data are consistent with the hypothesis that the introduction of a double-strand break at a specific site in the chromatin loops is an early event in apoptosis. This degradation is accompanied by morphologically observable changes in chromatin structure. Internucleosomal degradation, when it occurs, is a late event.</description><subject>Ageing, cell death</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - physiology</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell physiology</subject><subject>Cisplatin - pharmacology</subject><subject>DNA, Neoplasm - chemistry</subject><subject>DNA, Neoplasm - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microscopy, Electron</subject><subject>Molecular and cellular biology</subject><subject>Molecular Weight</subject><subject>Nucleosomes - drug effects</subject><subject>Nucleosomes - metabolism</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumor Cells, Cultured - metabolism</subject><subject>Tumor Cells, Cultured - pathology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE2PFCEQhonRrOPq1ZsJB-NtRmhoBo6TXnU3mbgXPZNqulox3dACvX78epnMZG-eKlAPbxUPIa8523HG1Hv87dKOGyPrUTdPyIYzw7aNbJqnZMMYl1upm_1z8iLnH4wxrbm6IleaS7FXekP-dj4vExQf6F0YVoeZHpa4lJh9pvUS6O06Q6D3D5B8rR0k50OcgXY4TfToA9JfvnyPa6FdDC7OvkAoNaxgCqubMOZKT_QGvyUY6qAYaBzpzefDS_JshCnjq0u9Jl8_fvjS3W6P95_uusNx66QSpX5llGZgwrgWQO4b2YI2jhmQA2-4E-ik6EFAz1ptVCsVF4Po29agEhJ7J67Ju3PukuLPFXOxs8-ubg8B45rtXsmGccMquDuDLsWcE452SX6G9MdyZk-y7Um2Pcm2J9n1wZtL8trPODziF7u1__bSh-xgGhME5_MjJnkjmVYV02cMq4UHj8lm5zE4HHxCV-wQ_f82-AeazZuS</recordid><startdate>19940401</startdate><enddate>19940401</enddate><creator>Ormerod, M.G.</creator><creator>O'Neill, C.F.</creator><creator>Robertson, D.</creator><creator>Harrap, K.R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940401</creationdate><title>Cisplatin Induces Apoptosis in a Human Ovarian Carcinoma Cell Line without Concomitant Internucleosomal Degradation of DNA</title><author>Ormerod, M.G. ; O'Neill, C.F. ; Robertson, D. ; Harrap, K.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-24f49d039c5aa47245a89c09a4d121c3ec43ba3ab0589654613d3b559e634ebc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Ageing, cell death</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - physiology</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell physiology</topic><topic>Cisplatin - pharmacology</topic><topic>DNA, Neoplasm - chemistry</topic><topic>DNA, Neoplasm - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Microscopy, Electron</topic><topic>Molecular and cellular biology</topic><topic>Molecular Weight</topic><topic>Nucleosomes - drug effects</topic><topic>Nucleosomes - metabolism</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Tumor Cells, Cultured - drug effects</topic><topic>Tumor Cells, Cultured - metabolism</topic><topic>Tumor Cells, Cultured - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ormerod, M.G.</creatorcontrib><creatorcontrib>O'Neill, C.F.</creatorcontrib><creatorcontrib>Robertson, D.</creatorcontrib><creatorcontrib>Harrap, K.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ormerod, M.G.</au><au>O'Neill, C.F.</au><au>Robertson, D.</au><au>Harrap, K.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cisplatin Induces Apoptosis in a Human Ovarian Carcinoma Cell Line without Concomitant Internucleosomal Degradation of DNA</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1994-04-01</date><risdate>1994</risdate><volume>211</volume><issue>2</issue><spage>231</spage><epage>237</epage><pages>231-237</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>After treatment of the human ovarian carcinoma cell line, CH1, with cisplatin, cells detached from the culture dish in a time- and dose-dependent fashion. These cells showed morphological changes indicative of apoptosis. Their DNA had not been degraded into oligonucleosomal fragments, but the DNA had been cut into larger fragments (30 kbp) of a size associated with chromatin loops. We conclude that cisplatin killed these ovarian cells by inducing apoptosis. However, in these cells, apoptosis was not accompanied by internucleosoreal degradation of DNA. Our data are consistent with the hypothesis that the introduction of a double-strand break at a specific site in the chromatin loops is an early event in apoptosis. This degradation is accompanied by morphologically observable changes in chromatin structure. Internucleosomal degradation, when it occurs, is a late event.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>8143768</pmid><doi>10.1006/excr.1994.1082</doi><tpages>7</tpages></addata></record> |
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| subjects | Ageing, cell death Apoptosis - drug effects Apoptosis - physiology Biological and medical sciences Cell Adhesion - drug effects Cell physiology Cisplatin - pharmacology DNA, Neoplasm - chemistry DNA, Neoplasm - metabolism Female Fundamental and applied biological sciences. Psychology Humans Microscopy, Electron Molecular and cellular biology Molecular Weight Nucleosomes - drug effects Nucleosomes - metabolism Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - metabolism Tumor Cells, Cultured - pathology |
| title | Cisplatin Induces Apoptosis in a Human Ovarian Carcinoma Cell Line without Concomitant Internucleosomal Degradation of DNA |
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