Evidence for Nickel in the Soluble Hydrogenase from the Unicellular Green Alga Scenedesmus obliquus
Cultures of the green alga Scenedesmus obliquus were grown in the presence of either the chelating reagent EDTA or NiCl in various concentrations and assayed for hydrogenase catalyzed photohydrogen evolution after an anaerobic dark adaptation period. Cultivation of algae in the presence of 100 μm ED...
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| Veröffentlicht in: | Zeitschrift für Naturforschung C. A journal of biosciences 1994-01, Vol.49 (1), p.33-38 |
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| container_title | Zeitschrift für Naturforschung C. A journal of biosciences |
| container_volume | 49 |
| creator | Zinn, Thomas Schnackenberg, Jörg Haak, Dieter Römer, Susanne Schulz, Rüdiger Senger, Horst |
| description | Cultures of the green alga Scenedesmus obliquus were grown in the presence of either the chelating reagent EDTA or NiCl
in various concentrations and assayed for hydrogenase catalyzed photohydrogen evolution after an anaerobic dark adaptation period. Cultivation of algae in the presence of 100 μm EDTA inhibited the formation of hydrogenase activity by 37%. After a cultivation of the cells in the presence of 5-20 μm NiCl
photohydrogen evolution was increased by 20-40% . Addition of EDTA up to a final concentration of 1.5 mᴍ had no effect on the activity of hydrogenase in cell-free hydrogenase preparations.
Cultures grown in the presence of radioactive
NiCl
incorporated
Ni in a parallel fashion to the cell growth. In radioactive labeled hydrogenase preparations a co-elution of radioactivity and hydrogenase activity could be observed using gel filtration chromatography. |
| doi_str_mv | 10.1515/znc-1994-1-206 |
| format | Article |
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in various concentrations and assayed for hydrogenase catalyzed photohydrogen evolution after an anaerobic dark adaptation period. Cultivation of algae in the presence of 100 μm EDTA inhibited the formation of hydrogenase activity by 37%. After a cultivation of the cells in the presence of 5-20 μm NiCl
photohydrogen evolution was increased by 20-40% . Addition of EDTA up to a final concentration of 1.5 mᴍ had no effect on the activity of hydrogenase in cell-free hydrogenase preparations.
Cultures grown in the presence of radioactive
NiCl
incorporated
Ni in a parallel fashion to the cell growth. In radioactive labeled hydrogenase preparations a co-elution of radioactivity and hydrogenase activity could be observed using gel filtration chromatography.</description><identifier>ISSN: 0939-5075</identifier><identifier>EISSN: 1865-7125</identifier><identifier>DOI: 10.1515/znc-1994-1-206</identifier><identifier>PMID: 8148007</identifier><identifier>CODEN: ZNCBDA</identifier><language>eng</language><publisher>Tübingen: Verlag der Zeitschrift für Naturforschung</publisher><subject>Anaerobiosis ; Biological and medical sciences ; Chlorophyta - enzymology ; Chlorophyta - growth & development ; Edetic Acid - pharmacology ; Enzymes ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Hydrogen - analysis ; Hydrogenase ; Hydrogenase - metabolism ; Kinetics ; Metabolism ; Nickel ; Nickel - metabolism ; Nickel - pharmacology ; Plant physiology and development ; Scenedesmus obliquus</subject><ispartof>Zeitschrift für Naturforschung C. A journal of biosciences, 1994-01, Vol.49 (1), p.33-38</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-8dae7c021cc381de35231665d460f009fbc10fd1eeac76130dd575d5025585a13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4116037$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8148007$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zinn, Thomas</creatorcontrib><creatorcontrib>Schnackenberg, Jörg</creatorcontrib><creatorcontrib>Haak, Dieter</creatorcontrib><creatorcontrib>Römer, Susanne</creatorcontrib><creatorcontrib>Schulz, Rüdiger</creatorcontrib><creatorcontrib>Senger, Horst</creatorcontrib><title>Evidence for Nickel in the Soluble Hydrogenase from the Unicellular Green Alga Scenedesmus obliquus</title><title>Zeitschrift für Naturforschung C. A journal of biosciences</title><addtitle>Z Naturforsch C</addtitle><description>Cultures of the green alga Scenedesmus obliquus were grown in the presence of either the chelating reagent EDTA or NiCl
in various concentrations and assayed for hydrogenase catalyzed photohydrogen evolution after an anaerobic dark adaptation period. Cultivation of algae in the presence of 100 μm EDTA inhibited the formation of hydrogenase activity by 37%. After a cultivation of the cells in the presence of 5-20 μm NiCl
photohydrogen evolution was increased by 20-40% . Addition of EDTA up to a final concentration of 1.5 mᴍ had no effect on the activity of hydrogenase in cell-free hydrogenase preparations.
Cultures grown in the presence of radioactive
NiCl
incorporated
Ni in a parallel fashion to the cell growth. In radioactive labeled hydrogenase preparations a co-elution of radioactivity and hydrogenase activity could be observed using gel filtration chromatography.</description><subject>Anaerobiosis</subject><subject>Biological and medical sciences</subject><subject>Chlorophyta - enzymology</subject><subject>Chlorophyta - growth & development</subject><subject>Edetic Acid - pharmacology</subject><subject>Enzymes</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen - analysis</subject><subject>Hydrogenase</subject><subject>Hydrogenase - metabolism</subject><subject>Kinetics</subject><subject>Metabolism</subject><subject>Nickel</subject><subject>Nickel - metabolism</subject><subject>Nickel - pharmacology</subject><subject>Plant physiology and development</subject><subject>Scenedesmus obliquus</subject><issn>0939-5075</issn><issn>1865-7125</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1vFDEQhi0ECpdAS4fkAtFt4ll_7G5BEUX5QIpCEVJbPnv22OC1E_scdPn18XGndIhqRnqfmbEfQj4BOwYJ8uQ52AaGQTTQtEy9IQvolWw6aOVbsmADHxrJOvmeHOZ8zxhXspMH5KAH0TPWLYg9f5ocBot0jIneTPY3ejoFuv6F9Db6svRIrzYuxRUGkyuV4vw3vAuTRe-LN4leJsRAT_3K0FuLAR3muWQal356LCV_IO9G4zN-3Ncjcndx_vPsqrn-cfn97PS6sUKIddM7g51lLVjLe3DIZctBKemEYiNjw7i0wEYHiMZ2Cjhzrn7GSdZK2UsD_Ih83e19SPGxYF7recrbR5qAsWTdKQFViPovWK_CAF1bweMdaFPMOeGoH9I0m7TRwPRWv6769Va_Bl3114HP-81lOaN7xfe-a_5ln5tsjR-TCXbKr5gAUIxvsW877I_xa0wOV6lsaqPvY0mhOvzHfTHUwjl_AfTDoY4</recordid><startdate>19940101</startdate><enddate>19940101</enddate><creator>Zinn, Thomas</creator><creator>Schnackenberg, Jörg</creator><creator>Haak, Dieter</creator><creator>Römer, Susanne</creator><creator>Schulz, Rüdiger</creator><creator>Senger, Horst</creator><general>Verlag der Zeitschrift für Naturforschung</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19940101</creationdate><title>Evidence for Nickel in the Soluble Hydrogenase from the Unicellular Green Alga Scenedesmus obliquus</title><author>Zinn, Thomas ; Schnackenberg, Jörg ; Haak, Dieter ; Römer, Susanne ; Schulz, Rüdiger ; Senger, Horst</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-8dae7c021cc381de35231665d460f009fbc10fd1eeac76130dd575d5025585a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Anaerobiosis</topic><topic>Biological and medical sciences</topic><topic>Chlorophyta - enzymology</topic><topic>Chlorophyta - growth & development</topic><topic>Edetic Acid - pharmacology</topic><topic>Enzymes</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen - analysis</topic><topic>Hydrogenase</topic><topic>Hydrogenase - metabolism</topic><topic>Kinetics</topic><topic>Metabolism</topic><topic>Nickel</topic><topic>Nickel - metabolism</topic><topic>Nickel - pharmacology</topic><topic>Plant physiology and development</topic><topic>Scenedesmus obliquus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zinn, Thomas</creatorcontrib><creatorcontrib>Schnackenberg, Jörg</creatorcontrib><creatorcontrib>Haak, Dieter</creatorcontrib><creatorcontrib>Römer, Susanne</creatorcontrib><creatorcontrib>Schulz, Rüdiger</creatorcontrib><creatorcontrib>Senger, Horst</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Zeitschrift für Naturforschung C. A journal of biosciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zinn, Thomas</au><au>Schnackenberg, Jörg</au><au>Haak, Dieter</au><au>Römer, Susanne</au><au>Schulz, Rüdiger</au><au>Senger, Horst</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for Nickel in the Soluble Hydrogenase from the Unicellular Green Alga Scenedesmus obliquus</atitle><jtitle>Zeitschrift für Naturforschung C. A journal of biosciences</jtitle><addtitle>Z Naturforsch C</addtitle><date>1994-01-01</date><risdate>1994</risdate><volume>49</volume><issue>1</issue><spage>33</spage><epage>38</epage><pages>33-38</pages><issn>0939-5075</issn><eissn>1865-7125</eissn><coden>ZNCBDA</coden><abstract>Cultures of the green alga Scenedesmus obliquus were grown in the presence of either the chelating reagent EDTA or NiCl
in various concentrations and assayed for hydrogenase catalyzed photohydrogen evolution after an anaerobic dark adaptation period. Cultivation of algae in the presence of 100 μm EDTA inhibited the formation of hydrogenase activity by 37%. After a cultivation of the cells in the presence of 5-20 μm NiCl
photohydrogen evolution was increased by 20-40% . Addition of EDTA up to a final concentration of 1.5 mᴍ had no effect on the activity of hydrogenase in cell-free hydrogenase preparations.
Cultures grown in the presence of radioactive
NiCl
incorporated
Ni in a parallel fashion to the cell growth. In radioactive labeled hydrogenase preparations a co-elution of radioactivity and hydrogenase activity could be observed using gel filtration chromatography.</abstract><cop>Tübingen</cop><pub>Verlag der Zeitschrift für Naturforschung</pub><pmid>8148007</pmid><doi>10.1515/znc-1994-1-206</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0939-5075 |
| ispartof | Zeitschrift für Naturforschung C. A journal of biosciences, 1994-01, Vol.49 (1), p.33-38 |
| issn | 0939-5075 1865-7125 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Anaerobiosis Biological and medical sciences Chlorophyta - enzymology Chlorophyta - growth & development Edetic Acid - pharmacology Enzymes Freshwater Fundamental and applied biological sciences. Psychology Hydrogen - analysis Hydrogenase Hydrogenase - metabolism Kinetics Metabolism Nickel Nickel - metabolism Nickel - pharmacology Plant physiology and development Scenedesmus obliquus |
| title | Evidence for Nickel in the Soluble Hydrogenase from the Unicellular Green Alga Scenedesmus obliquus |
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