Transcription of repeat DNA in vitro by RNA polymerase III in cytosol extracts is inhibited when the extract is of the same species as the DNA
Cytosol extracts containing RNA Polymerase III were obtained from cultured human (HeLa) and bovine (MDBK) cells. Human extract efficiently transcribed genomic bovine DNA and cloned bovine alu-type repeat DNA while genomic human DNA and cloned human alu repeat DNA were inefficient templates. Converse...
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Veröffentlicht in: | Biochemical and biophysical research communications 1985-09, Vol.131 (2), p.551-556 |
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description | Cytosol extracts containing RNA Polymerase III were obtained from cultured human (HeLa) and bovine (MDBK) cells. Human extract efficiently transcribed genomic bovine DNA and cloned bovine alu-type repeat DNA while genomic human DNA and cloned human alu repeat DNA were inefficient templates. Conversely, genomic bovine DNA and cloned bovine alu-type repeat DNA were less effective templates for bovine extract than were genomic human DNA and alu repeat DNA. These results suggest that species specific factor(s) present in mammalian cells are responsible for the observed in vivo inhibition of transcription of most alu-type repeat DNA. |
doi_str_mv | 10.1016/0006-291X(85)91271-9 |
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J</creator><creatorcontrib>FURTH, J. J</creatorcontrib><description>Cytosol extracts containing RNA Polymerase III were obtained from cultured human (HeLa) and bovine (MDBK) cells. Human extract efficiently transcribed genomic bovine DNA and cloned bovine alu-type repeat DNA while genomic human DNA and cloned human alu repeat DNA were inefficient templates. Conversely, genomic bovine DNA and cloned bovine alu-type repeat DNA were less effective templates for bovine extract than were genomic human DNA and alu repeat DNA. These results suggest that species specific factor(s) present in mammalian cells are responsible for the observed in vivo inhibition of transcription of most alu-type repeat DNA.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(85)91271-9</identifier><identifier>PMID: 2413845</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier</publisher><subject>Animals ; Applied sciences ; Cattle ; Cell Line ; Cytosol - enzymology ; DNA - metabolism ; DNA-Directed RNA Polymerases - metabolism ; Exact sciences and technology ; HeLa Cells - enzymology ; Humans ; Kidney ; Other techniques and industries ; Repetitive Sequences, Nucleic Acid ; RNA - biosynthesis ; RNA Polymerase III - metabolism ; Species Specificity ; Templates, Genetic ; Transcription, Genetic</subject><ispartof>Biochemical and biophysical research communications, 1985-09, Vol.131 (2), p.551-556</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7974931$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2413845$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FURTH, J. 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These results suggest that species specific factor(s) present in mammalian cells are responsible for the observed in vivo inhibition of transcription of most alu-type repeat DNA.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Cattle</subject><subject>Cell Line</subject><subject>Cytosol - enzymology</subject><subject>DNA - metabolism</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Exact sciences and technology</subject><subject>HeLa Cells - enzymology</subject><subject>Humans</subject><subject>Kidney</subject><subject>Other techniques and industries</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RNA - biosynthesis</subject><subject>RNA Polymerase III - metabolism</subject><subject>Species Specificity</subject><subject>Templates, Genetic</subject><subject>Transcription, Genetic</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF1LXDEQhoO02NX2H1jIRZH24tiZbE7OyaVorQuiUCz0bsnHLBs5XyZZ2_0T_uZm61YYZpj3eZkXhrEThDMEVF8BQFVC46_Pbf1Fo2iw0gdshqChEgjyDZu9Wt6xo5QeABCl0ofsUEict7Kesef7aIbkYphyGAc-rnikiUzml7fnPAz8KeQ4crvlP8o-jd22p2gS8cViscNum8c0dpz-5GhcTjyUGtbBhkye_17TwPOa_uMdLQk7JZm-tIlcoMRN-qeVyPfs7cp0iT7s5zH7efXt_uK6urn7vrg4v6kmrCFXUtna1VZ4sSKyxqETyoK3AlrUSnpZO9N60roIVnkDUFPTNGA8ghdYz4_Z6cvdKY6PG0p52YfkqOvMQOMmLRslUbVCF-PHvXFje_LLKYbexO1y_8DCP-25Sc50q_JNF9KrrdGN1HOc_wUxq4CX</recordid><startdate>19850916</startdate><enddate>19850916</enddate><creator>FURTH, J. 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J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p150t-46b5c5b2d2feebac1c26b0db2081964d45ca8de99208b6da005e7770ad10d2153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Cattle</topic><topic>Cell Line</topic><topic>Cytosol - enzymology</topic><topic>DNA - metabolism</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Exact sciences and technology</topic><topic>HeLa Cells - enzymology</topic><topic>Humans</topic><topic>Kidney</topic><topic>Other techniques and industries</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>RNA - biosynthesis</topic><topic>RNA Polymerase III - metabolism</topic><topic>Species Specificity</topic><topic>Templates, Genetic</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FURTH, J. 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J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcription of repeat DNA in vitro by RNA polymerase III in cytosol extracts is inhibited when the extract is of the same species as the DNA</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1985-09-16</date><risdate>1985</risdate><volume>131</volume><issue>2</issue><spage>551</spage><epage>556</epage><pages>551-556</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Cytosol extracts containing RNA Polymerase III were obtained from cultured human (HeLa) and bovine (MDBK) cells. Human extract efficiently transcribed genomic bovine DNA and cloned bovine alu-type repeat DNA while genomic human DNA and cloned human alu repeat DNA were inefficient templates. Conversely, genomic bovine DNA and cloned bovine alu-type repeat DNA were less effective templates for bovine extract than were genomic human DNA and alu repeat DNA. These results suggest that species specific factor(s) present in mammalian cells are responsible for the observed in vivo inhibition of transcription of most alu-type repeat DNA.</abstract><cop>San Diego, CA</cop><pub>Elsevier</pub><pmid>2413845</pmid><doi>10.1016/0006-291X(85)91271-9</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Applied sciences Cattle Cell Line Cytosol - enzymology DNA - metabolism DNA-Directed RNA Polymerases - metabolism Exact sciences and technology HeLa Cells - enzymology Humans Kidney Other techniques and industries Repetitive Sequences, Nucleic Acid RNA - biosynthesis RNA Polymerase III - metabolism Species Specificity Templates, Genetic Transcription, Genetic |
title | Transcription of repeat DNA in vitro by RNA polymerase III in cytosol extracts is inhibited when the extract is of the same species as the DNA |
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