A direct, sensitive microassay for mammalian histidine decarboxylase
A microassay procedure for mammalian histidine decarboxylase based on the conversion of l-[ 3H]histidine to [ 3H]histamine, which were separated by an alkaline butanol extraction followed by thin-layer chromatography, is described. This assay is direct and simple to perform, in addition to being ver...
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| Veröffentlicht in: | Biochemical pharmacology 1985-10, Vol.34 (20), p.3711-3716 |
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| container_end_page | 3716 |
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| container_issue | 20 |
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| container_title | Biochemical pharmacology |
| container_volume | 34 |
| creator | Hegstrand, Linda R. |
| description | A microassay procedure for mammalian histidine decarboxylase based on the conversion of
l-[
3H]histidine to [
3H]histamine, which were separated by an alkaline butanol extraction followed by thin-layer chromatography, is described. This assay is direct and simple to perform, in addition to being very sensitive and reproducible. It is useful for tissues containing high levels of endogenous histamine, because only newly formed radiolabeled histamine is measured. This report includes information on histidine decarboxylase activity at various pH levels, in different buffers, and in the presence of selected histamine active drugs. In addition, it describes histidine decarboxylase activity in several fetal rat tissues. |
| doi_str_mv | 10.1016/0006-2952(85)90235-7 |
| format | Article |
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l-[
3H]histidine to [
3H]histamine, which were separated by an alkaline butanol extraction followed by thin-layer chromatography, is described. This assay is direct and simple to perform, in addition to being very sensitive and reproducible. It is useful for tissues containing high levels of endogenous histamine, because only newly formed radiolabeled histamine is measured. This report includes information on histidine decarboxylase activity at various pH levels, in different buffers, and in the presence of selected histamine active drugs. In addition, it describes histidine decarboxylase activity in several fetal rat tissues.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/0006-2952(85)90235-7</identifier><identifier>PMID: 4052109</identifier><identifier>CODEN: BCPCA6</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Carboxy-Lyases - analysis ; Chromatography, Thin Layer ; Enzymes and enzyme inhibitors ; Fetus ; Fundamental and applied biological sciences. Psychology ; Histamine - metabolism ; Histidine - metabolism ; Histidine Decarboxylase - analysis ; Histidine Decarboxylase - metabolism ; Hydrogen-Ion Concentration ; Liver - enzymology ; Lyases ; Rats ; Rats, Inbred Strains ; Tritium</subject><ispartof>Biochemical pharmacology, 1985-10, Vol.34 (20), p.3711-3716</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-4c8d8e03c652bd8c88ef5c856961a1631cb8f5aca4ac8a6ce5f7a759da4963d63</citedby><cites>FETCH-LOGICAL-c386t-4c8d8e03c652bd8c88ef5c856961a1631cb8f5aca4ac8a6ce5f7a759da4963d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006295285902357$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8707903$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4052109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hegstrand, Linda R.</creatorcontrib><title>A direct, sensitive microassay for mammalian histidine decarboxylase</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>A microassay procedure for mammalian histidine decarboxylase based on the conversion of
l-[
3H]histidine to [
3H]histamine, which were separated by an alkaline butanol extraction followed by thin-layer chromatography, is described. This assay is direct and simple to perform, in addition to being very sensitive and reproducible. It is useful for tissues containing high levels of endogenous histamine, because only newly formed radiolabeled histamine is measured. This report includes information on histidine decarboxylase activity at various pH levels, in different buffers, and in the presence of selected histamine active drugs. In addition, it describes histidine decarboxylase activity in several fetal rat tissues.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carboxy-Lyases - analysis</subject><subject>Chromatography, Thin Layer</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histamine - metabolism</subject><subject>Histidine - metabolism</subject><subject>Histidine Decarboxylase - analysis</subject><subject>Histidine Decarboxylase - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Liver - enzymology</subject><subject>Lyases</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Tritium</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtL7DAUh4Nc0fHxHyh0IaJgNWmbNN0I4hsEN7oOZ05Oubn0oTkdcf57W2eY5V2F5PedRz4hjpS8VFKZKymlSbNKZ2dWn1cyy3VabomZsmU-Phv7R8w2yK7YY_43Xa1RO2KnkDpTspqJu5vEh0g4XCRMHYchfFHSBow9MMMyqfuYtNC20ATokr-Bh-BDR4knhDjvv5cNMB2I7RoapsP1uS_eH-7fbp_Sl9fH59ublxRza4a0QOstyRyNzubeorVUa7TaVEaBMrnCua01IBSAFgySrksodeWhqEzuTb4vTld9P2L_uSAeXBsYqWmgo37BrjSFMrrKR7BYgeM_mCPV7iOGFuLSKekmeW5S4SYzzmr3K8-VY9nxuv9i3pLfFK1tjfnJOgdGaOoIHQbeYLaUZSWn6dcrjEYXX4GiYwzUIa1MO9-H_-_xA3VFiy0</recordid><startdate>19851015</startdate><enddate>19851015</enddate><creator>Hegstrand, Linda R.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19851015</creationdate><title>A direct, sensitive microassay for mammalian histidine decarboxylase</title><author>Hegstrand, Linda R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-4c8d8e03c652bd8c88ef5c856961a1631cb8f5aca4ac8a6ce5f7a759da4963d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carboxy-Lyases - analysis</topic><topic>Chromatography, Thin Layer</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histamine - metabolism</topic><topic>Histidine - metabolism</topic><topic>Histidine Decarboxylase - analysis</topic><topic>Histidine Decarboxylase - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Liver - enzymology</topic><topic>Lyases</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hegstrand, Linda R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hegstrand, Linda R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A direct, sensitive microassay for mammalian histidine decarboxylase</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>1985-10-15</date><risdate>1985</risdate><volume>34</volume><issue>20</issue><spage>3711</spage><epage>3716</epage><pages>3711-3716</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><coden>BCPCA6</coden><abstract>A microassay procedure for mammalian histidine decarboxylase based on the conversion of
l-[
3H]histidine to [
3H]histamine, which were separated by an alkaline butanol extraction followed by thin-layer chromatography, is described. This assay is direct and simple to perform, in addition to being very sensitive and reproducible. It is useful for tissues containing high levels of endogenous histamine, because only newly formed radiolabeled histamine is measured. This report includes information on histidine decarboxylase activity at various pH levels, in different buffers, and in the presence of selected histamine active drugs. In addition, it describes histidine decarboxylase activity in several fetal rat tissues.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>4052109</pmid><doi>10.1016/0006-2952(85)90235-7</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical pharmacology, 1985-10, Vol.34 (20), p.3711-3716 |
| issn | 0006-2952 1873-2968 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Carboxy-Lyases - analysis Chromatography, Thin Layer Enzymes and enzyme inhibitors Fetus Fundamental and applied biological sciences. Psychology Histamine - metabolism Histidine - metabolism Histidine Decarboxylase - analysis Histidine Decarboxylase - metabolism Hydrogen-Ion Concentration Liver - enzymology Lyases Rats Rats, Inbred Strains Tritium |
| title | A direct, sensitive microassay for mammalian histidine decarboxylase |
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