[26] Detection of O-Linked N-acetylglucosamine (O-GlcNAc) on cytoplasmic and nuclear proteins

This chapter describes methods for the detection and initial analysis of O-linked N-acetylglucosamine (O-GlcNAc) on proteins. Although the galactosyltransferase/UDP[3H]galactose probe method is highly specific it requires amounts of protein in the low microgram range. The chapter also describes a co...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Methods in Enzymology 1994, Vol.230, p.443-460
Hauptverfasser:	Roquemore, Elizabeth P., Chou, Teh-Ying, Hart, Gerald W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosamine - analysis Amino Acid Sequence Animals Blotting, Western - methods Cattle Chromatography, Affinity - methods Chromatography, High Pressure Liquid - methods Cytoplasm - metabolism Galactose - metabolism Galactosyltransferases Glycopeptides - chemistry Glycoproteins - biosynthesis Glycoproteins - chemistry Glycoproteins - isolation & purification Glycoside Hydrolases Humans Indicators and Reagents Molecular Sequence Data Nuclear Proteins - biosynthesis Nuclear Proteins - chemistry Nuclear Proteins - isolation & purification Peptide Fragments - chemistry Radioisotope Dilution Technique Rats Tritium
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!