Neutrophil Activation by Monomeric Interleukin-8
Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectively block formation of hydrogen bonds between mono...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1994-04, Vol.264 (5155), p.90-92 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Rajarathnam, Krishnakumar Sykes, Brian D. Kay, Cyril M. Dewald, Beatrice Geiser, Thomas Baggiolini, Marco Clark-Lewis, Ian |
| description | Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectively block formation of hydrogen bonds between monomers and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8. |
| doi_str_mv | 10.1126/science.8140420 |
| format | Article |
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An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectively block formation of hydrogen bonds between monomers and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.8140420</identifier><identifier>PMID: 8140420</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Amides ; Analytical, structural and metabolic biochemistry ; Biochemistry ; Biological and medical sciences ; Calcium - metabolism ; Chemistry ; Chemotaxis, Leukocyte ; Dimers ; Drugs ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrogen Bonding ; Interleukin-8 - analogs & derivatives ; Interleukin-8 - chemistry ; Interleukin-8 - metabolism ; Interleukin-8 - pharmacology ; Interleukins ; Leukocyte Elastase ; Models, Chemical ; Molecular weight ; Monomers ; Neutrophil activation ; Neutrophils ; Neutrophils - drug effects ; Neutrophils - physiology ; Nuclear magnetic resonance ; Pancreatic Elastase - metabolism ; Protein Conformation ; Protein hormones. Growth factors. Cytokines ; Protein Structure, Secondary ; Proteins ; Protons ; Receptors ; Receptors, Interleukin - chemistry ; Receptors, Interleukin - metabolism ; Receptors, Interleukin-8A ; Topology</subject><ispartof>Science (American Association for the Advancement of Science), 1994-04, Vol.264 (5155), p.90-92</ispartof><rights>Copyright 1994 American Association for the Advancement of Science</rights><rights>1994 INIST-CNRS</rights><rights>COPYRIGHT 1994 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1994 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Apr 1, 1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c673t-92cbf810296c8c899bf6bcdc8f969be39382a14afe2a3e0bfbc377b7eb1b65c43</citedby><cites>FETCH-LOGICAL-c673t-92cbf810296c8c899bf6bcdc8f969be39382a14afe2a3e0bfbc377b7eb1b65c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2883829$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2883829$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4009376$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8140420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rajarathnam, Krishnakumar</creatorcontrib><creatorcontrib>Sykes, Brian D.</creatorcontrib><creatorcontrib>Kay, Cyril M.</creatorcontrib><creatorcontrib>Dewald, Beatrice</creatorcontrib><creatorcontrib>Geiser, Thomas</creatorcontrib><creatorcontrib>Baggiolini, Marco</creatorcontrib><creatorcontrib>Clark-Lewis, Ian</creatorcontrib><title>Neutrophil Activation by Monomeric Interleukin-8</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectively block formation of hydrogen bonds between monomers and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8.</description><subject>Amides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Chemistry</subject><subject>Chemotaxis, Leukocyte</subject><subject>Dimers</subject><subject>Drugs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrogen Bonding</subject><subject>Interleukin-8 - analogs & derivatives</subject><subject>Interleukin-8 - chemistry</subject><subject>Interleukin-8 - metabolism</subject><subject>Interleukin-8 - pharmacology</subject><subject>Interleukins</subject><subject>Leukocyte Elastase</subject><subject>Models, Chemical</subject><subject>Molecular weight</subject><subject>Monomers</subject><subject>Neutrophil activation</subject><subject>Neutrophils</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - physiology</subject><subject>Nuclear magnetic resonance</subject><subject>Pancreatic Elastase - metabolism</subject><subject>Protein Conformation</subject><subject>Protein hormones. Growth factors. Cytokines</subject><subject>Protein Structure, Secondary</subject><subject>Proteins</subject><subject>Protons</subject><subject>Receptors</subject><subject>Receptors, Interleukin - chemistry</subject><subject>Receptors, Interleukin - metabolism</subject><subject>Receptors, Interleukin-8A</subject><subject>Topology</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0s-LEzEUB_AgytpdPXtRKCLiYWc3mcxkMsdatBbq9uCPa0jSl5o6k9RkZnH_e1M6VCoFSw6BvE_CC--L0AuCbwjJ2W3UFpyGG04KXOT4ERoRXJdZnWP6GI0wpizjuCqfossYNxinWk0v0MXARwjfQd8Fv_1hm_FEd_Zedta7sXoYf_bOtxCsHs9dB6GB_qd1GX-GnhjZRHg-7Ffo28cPX6efssVyNp9OFplmFe1SA1oZTnBeM801r2tlmNIrzU3NagW0pjyXpJAGckkBK6M0rSpVgSKKlbqgV-jt_t1t8L96iJ1obdTQNNKB76OoWEEIL9h_IWGc8JLtXnz9D9z4Prj0CZETWpZVXuCErvdoLRsQ1hnfBanX4CDIxjswNh1PSElxznmeeHaCp7WC1upT_t2RT6SD391a9jGK-Ze7s-ny-9n0_excymeLI3p9imrfNLAGkaY9XR7x2z3XwccYwIhtsK0MD4JgscuqGLIqhvClG6-GgfSqhdXB_62_GeoyatmYIJ228cCKlGZa7QLwcs82sfPhUE5NpYzV9A9TwPWf</recordid><startdate>19940401</startdate><enddate>19940401</enddate><creator>Rajarathnam, Krishnakumar</creator><creator>Sykes, Brian D.</creator><creator>Kay, Cyril M.</creator><creator>Dewald, Beatrice</creator><creator>Geiser, Thomas</creator><creator>Baggiolini, Marco</creator><creator>Clark-Lewis, Ian</creator><general>American Society for the Advancement of Science</general><general>American Association for the Advancement of Science</general><general>The American Association for the Advancement of Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>0-V</scope><scope>3V.</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88B</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CJNVE</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9-</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0K</scope><scope>M0P</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEDU</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>7T5</scope><scope>7X8</scope></search><sort><creationdate>19940401</creationdate><title>Neutrophil Activation by Monomeric Interleukin-8</title><author>Rajarathnam, Krishnakumar ; Sykes, Brian D. ; Kay, Cyril M. ; Dewald, Beatrice ; Geiser, Thomas ; Baggiolini, Marco ; Clark-Lewis, Ian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c673t-92cbf810296c8c899bf6bcdc8f969be39382a14afe2a3e0bfbc377b7eb1b65c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Chemistry</topic><topic>Chemotaxis, Leukocyte</topic><topic>Dimers</topic><topic>Drugs</topic><topic>Fundamental and applied biological sciences. 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Science)</jtitle><addtitle>Science</addtitle><date>1994-04-01</date><risdate>1994</risdate><volume>264</volume><issue>5155</issue><spage>90</spage><epage>92</epage><pages>90-92</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Interleukin-8 (IL-8), a pro-inflammatory protein, has been shown by nuclear magnetic resonance (NMR) and x-ray techniques to exist as a homodimer. An IL-8 analog was chemically synthesized, with the amide nitrogen of leucine-25 methylated to selectively block formation of hydrogen bonds between monomers and thereby prevent dimerization. This analog was shown to be a monomer, as assessed by analytical ultracentrifugation and NMR. Nevertheless, it was equivalent to IL-8 in assays of neutrophil activation, which indicates that the monomer is a functional form of IL-8.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>8140420</pmid><doi>10.1126/science.8140420</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1994-04, Vol.264 (5155), p.90-92 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76411846 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Amides Analytical, structural and metabolic biochemistry Biochemistry Biological and medical sciences Calcium - metabolism Chemistry Chemotaxis, Leukocyte Dimers Drugs Fundamental and applied biological sciences. Psychology Humans Hydrogen Bonding Interleukin-8 - analogs & derivatives Interleukin-8 - chemistry Interleukin-8 - metabolism Interleukin-8 - pharmacology Interleukins Leukocyte Elastase Models, Chemical Molecular weight Monomers Neutrophil activation Neutrophils Neutrophils - drug effects Neutrophils - physiology Nuclear magnetic resonance Pancreatic Elastase - metabolism Protein Conformation Protein hormones. Growth factors. Cytokines Protein Structure, Secondary Proteins Protons Receptors Receptors, Interleukin - chemistry Receptors, Interleukin - metabolism Receptors, Interleukin-8A Topology |
| title | Neutrophil Activation by Monomeric Interleukin-8 |
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