Utilization of mannose by astroglial cells
Uptake and metabolism of mannose were studied in astroglia-rich primary cultures derived from neonatal rat brains. A saturable component of mannose uptake was found with half-maximal uptake at 6.7 +/- 1.0 mM mannose. In addition, a non-saturable component dominated the uptake at high concentrations...
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| Veröffentlicht in: | Neurochemical research 1994, Vol.19 (1), p.23-30 |
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| creator | DRINGEN, R BERGBAUER, K WIESINGER, H HAMPRECHT, B |
| description | Uptake and metabolism of mannose were studied in astroglia-rich primary cultures derived from neonatal rat brains. A saturable component of mannose uptake was found with half-maximal uptake at 6.7 +/- 1.0 mM mannose. In addition, a non-saturable component dominated the uptake at high concentrations of mannose. Glucose, cytochalasin B, or phloretin in the incubation buffer inhibited the carrier-mediated uptake of mannose. Within the astroglial cells mannose is phosphorylated to mannose-6-phosphate. In cell homogenates, the KM value of mannose-phosphorylating activity was determined to be 24 +/- 7 microM. The Vmax value of this activity is only 40% that of glucose-phosphorylating activity. Mannose-6-phosphate was converted to fructose-6-phosphate by mannose-6-phosphate isomerase. The specific activity of this enzyme in homogenates of astroglial cultures was higher than that of hexokinase. Two products of mannose utilization in astroglial cells are glycogen and lactate. The amounts of each of these products increased with increasing concentrations of mannose. In contrast to the generation of lactate, that of glycogen from mannose was enhanced in the presence of insulin. In conclusion, we suggest that mannose is taken up into the cells of astroglia-rich primary cultures by the glial glucose transporter and is metabolized to fructose-6-phosphate within the astroglial cells. |
| doi_str_mv | 10.1007/BF00966724 |
| format | Article |
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A saturable component of mannose uptake was found with half-maximal uptake at 6.7 +/- 1.0 mM mannose. In addition, a non-saturable component dominated the uptake at high concentrations of mannose. Glucose, cytochalasin B, or phloretin in the incubation buffer inhibited the carrier-mediated uptake of mannose. Within the astroglial cells mannose is phosphorylated to mannose-6-phosphate. In cell homogenates, the KM value of mannose-phosphorylating activity was determined to be 24 +/- 7 microM. The Vmax value of this activity is only 40% that of glucose-phosphorylating activity. Mannose-6-phosphate was converted to fructose-6-phosphate by mannose-6-phosphate isomerase. The specific activity of this enzyme in homogenates of astroglial cultures was higher than that of hexokinase. Two products of mannose utilization in astroglial cells are glycogen and lactate. The amounts of each of these products increased with increasing concentrations of mannose. In contrast to the generation of lactate, that of glycogen from mannose was enhanced in the presence of insulin. In conclusion, we suggest that mannose is taken up into the cells of astroglia-rich primary cultures by the glial glucose transporter and is metabolized to fructose-6-phosphate within the astroglial cells.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/BF00966724</identifier><identifier>PMID: 8139758</identifier><identifier>CODEN: NEREDZ</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Animals ; Animals, Newborn ; Astrocytes - drug effects ; Astrocytes - metabolism ; Biological and medical sciences ; Biological Transport - drug effects ; Brain - metabolism ; Cells, Cultured ; Cytochalasin B - pharmacology ; Fundamental and applied biological sciences. Psychology ; Glucose - pharmacology ; Glycogen - metabolism ; Hexokinase - metabolism ; Isolated neuron and nerve. Neuroglia ; Kinetics ; Lactates - analysis ; Mannose - metabolism ; Mannose-6-Phosphate Isomerase - metabolism ; Phloretin - pharmacology ; Rats ; Rats, Wistar ; Vertebrates: nervous system and sense organs</subject><ispartof>Neurochemical research, 1994, Vol.19 (1), p.23-30</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-15a7a8333824b1a6ce21f7bef02aac0a826421aef648220018f9991b05dbd203</citedby><cites>FETCH-LOGICAL-c342t-15a7a8333824b1a6ce21f7bef02aac0a826421aef648220018f9991b05dbd203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,4012,27910,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4086821$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8139758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DRINGEN, R</creatorcontrib><creatorcontrib>BERGBAUER, K</creatorcontrib><creatorcontrib>WIESINGER, H</creatorcontrib><creatorcontrib>HAMPRECHT, B</creatorcontrib><title>Utilization of mannose by astroglial cells</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><description>Uptake and metabolism of mannose were studied in astroglia-rich primary cultures derived from neonatal rat brains. A saturable component of mannose uptake was found with half-maximal uptake at 6.7 +/- 1.0 mM mannose. In addition, a non-saturable component dominated the uptake at high concentrations of mannose. Glucose, cytochalasin B, or phloretin in the incubation buffer inhibited the carrier-mediated uptake of mannose. Within the astroglial cells mannose is phosphorylated to mannose-6-phosphate. In cell homogenates, the KM value of mannose-phosphorylating activity was determined to be 24 +/- 7 microM. The Vmax value of this activity is only 40% that of glucose-phosphorylating activity. Mannose-6-phosphate was converted to fructose-6-phosphate by mannose-6-phosphate isomerase. The specific activity of this enzyme in homogenates of astroglial cultures was higher than that of hexokinase. Two products of mannose utilization in astroglial cells are glycogen and lactate. The amounts of each of these products increased with increasing concentrations of mannose. In contrast to the generation of lactate, that of glycogen from mannose was enhanced in the presence of insulin. In conclusion, we suggest that mannose is taken up into the cells of astroglia-rich primary cultures by the glial glucose transporter and is metabolized to fructose-6-phosphate within the astroglial cells.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Astrocytes - drug effects</subject><subject>Astrocytes - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Brain - metabolism</subject><subject>Cells, Cultured</subject><subject>Cytochalasin B - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - pharmacology</subject><subject>Glycogen - metabolism</subject><subject>Hexokinase - metabolism</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Kinetics</subject><subject>Lactates - analysis</subject><subject>Mannose - metabolism</subject><subject>Mannose-6-Phosphate Isomerase - metabolism</subject><subject>Phloretin - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0L9Lw0AUB_BDlFqri7uQQRwK0Xd3uV-jFqtCwaXO4SW9k0iSq3fJUP96Uxrq6PSG9-HLly8h1xTuKYB6eFoCGCkVy07IlArFU2mAn5IpcJmlnBo4JxcxfgEMnNEJmWjKjRJ6SuYfXVVXP9hVvk28SxpsWx9tUuwSjF3wn3WFdVLauo6X5MxhHe3VeGdkvXxeL17T1fvL2-JxlZY8Y11KBSrUnHPNsoKiLC2jThXWAUMsATWTGaNoncw0Y0Ml7YwxtACxKTYM-IzcHWK3wX_3NnZ5U8V9AWyt72OuZAaaafEvpFILIbQc4PwAy-BjDNbl21A1GHY5hXw_YP434IBvxtS-aOzmSMfFhv_t-MdYYu0CtmUVj2zoJjWj_BeIYHUP</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>DRINGEN, R</creator><creator>BERGBAUER, K</creator><creator>WIESINGER, H</creator><creator>HAMPRECHT, B</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Utilization of mannose by astroglial cells</title><author>DRINGEN, R ; BERGBAUER, K ; WIESINGER, H ; HAMPRECHT, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-15a7a8333824b1a6ce21f7bef02aac0a826421aef648220018f9991b05dbd203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Astrocytes - drug effects</topic><topic>Astrocytes - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Brain - metabolism</topic><topic>Cells, Cultured</topic><topic>Cytochalasin B - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - pharmacology</topic><topic>Glycogen - metabolism</topic><topic>Hexokinase - metabolism</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Kinetics</topic><topic>Lactates - analysis</topic><topic>Mannose - metabolism</topic><topic>Mannose-6-Phosphate Isomerase - metabolism</topic><topic>Phloretin - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DRINGEN, R</creatorcontrib><creatorcontrib>BERGBAUER, K</creatorcontrib><creatorcontrib>WIESINGER, H</creatorcontrib><creatorcontrib>HAMPRECHT, B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neurochemical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DRINGEN, R</au><au>BERGBAUER, K</au><au>WIESINGER, H</au><au>HAMPRECHT, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utilization of mannose by astroglial cells</atitle><jtitle>Neurochemical research</jtitle><addtitle>Neurochem Res</addtitle><date>1994</date><risdate>1994</risdate><volume>19</volume><issue>1</issue><spage>23</spage><epage>30</epage><pages>23-30</pages><issn>0364-3190</issn><eissn>1573-6903</eissn><coden>NEREDZ</coden><abstract>Uptake and metabolism of mannose were studied in astroglia-rich primary cultures derived from neonatal rat brains. A saturable component of mannose uptake was found with half-maximal uptake at 6.7 +/- 1.0 mM mannose. In addition, a non-saturable component dominated the uptake at high concentrations of mannose. Glucose, cytochalasin B, or phloretin in the incubation buffer inhibited the carrier-mediated uptake of mannose. Within the astroglial cells mannose is phosphorylated to mannose-6-phosphate. In cell homogenates, the KM value of mannose-phosphorylating activity was determined to be 24 +/- 7 microM. The Vmax value of this activity is only 40% that of glucose-phosphorylating activity. Mannose-6-phosphate was converted to fructose-6-phosphate by mannose-6-phosphate isomerase. The specific activity of this enzyme in homogenates of astroglial cultures was higher than that of hexokinase. Two products of mannose utilization in astroglial cells are glycogen and lactate. The amounts of each of these products increased with increasing concentrations of mannose. In contrast to the generation of lactate, that of glycogen from mannose was enhanced in the presence of insulin. In conclusion, we suggest that mannose is taken up into the cells of astroglia-rich primary cultures by the glial glucose transporter and is metabolized to fructose-6-phosphate within the astroglial cells.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>8139758</pmid><doi>10.1007/BF00966724</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Animals, Newborn Astrocytes - drug effects Astrocytes - metabolism Biological and medical sciences Biological Transport - drug effects Brain - metabolism Cells, Cultured Cytochalasin B - pharmacology Fundamental and applied biological sciences. Psychology Glucose - pharmacology Glycogen - metabolism Hexokinase - metabolism Isolated neuron and nerve. Neuroglia Kinetics Lactates - analysis Mannose - metabolism Mannose-6-Phosphate Isomerase - metabolism Phloretin - pharmacology Rats Rats, Wistar Vertebrates: nervous system and sense organs |
| title | Utilization of mannose by astroglial cells |
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