Endothelin-1 Stimulates Cytosolic Phospholipase A2 in Chinese Hamster Ovary Cells Stably Expressing the Human ETA or ETB Receptor Subtype
The ability of endothelin-1 (ET-1) to activate cytosolic phospholipase A2 (cPLA2) has been studied in Chinese Hamster ovary (CHO) cells stably expressing either the human ETA (CHO/ETA) or the human ETB (CHO/ETB) receptor subtype. ET-1 dose-dependently increased a dithiothreitol-insensitive cPLA2 act...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1994-03, Vol.199 (2), p.992-997 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Schramek, H. Wang, Y. Konieczkowski, M. Rose, P.M. Sedor, J.R. Dunn, M.J. |
| description | The ability of endothelin-1 (ET-1) to activate cytosolic phospholipase A2 (cPLA2) has been studied in Chinese Hamster ovary (CHO) cells stably expressing either the human ETA (CHO/ETA) or the human ETB (CHO/ETB) receptor subtype. ET-1 dose-dependently increased a dithiothreitol-insensitive cPLA2 activity in both cell types. In CHO/ETA cells, BQ-123, an ETA-selective antagonist, completely blocked ET-1-induced cPLA2 stimulation. In CHO/ETB cells, the ET-1 response was mimicked by 4AlaET-1 which could be blocked partially by PD 145065, a nonselective antagonist of ETA and ETB. As expected, ET-1 stimulated PGE2 synthesis in CHO cells transfected with ET receptors. We conclude that ET-1 can stimulate cPLA2 via both the human ETA and ETB receptor subtype. |
| doi_str_mv | 10.1006/bbrc.1994.1327 |
| format | Article |
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ET-1 dose-dependently increased a dithiothreitol-insensitive cPLA2 activity in both cell types. In CHO/ETA cells, BQ-123, an ETA-selective antagonist, completely blocked ET-1-induced cPLA2 stimulation. In CHO/ETB cells, the ET-1 response was mimicked by 4AlaET-1 which could be blocked partially by PD 145065, a nonselective antagonist of ETA and ETB. As expected, ET-1 stimulated PGE2 synthesis in CHO cells transfected with ET receptors. We conclude that ET-1 can stimulate cPLA2 via both the human ETA and ETB receptor subtype.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1994.1327</identifier><identifier>PMID: 8135848</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Blotting, Western ; CHO Cells ; Cricetinae ; Cytosol - enzymology ; Dinoprostone - metabolism ; Dithiothreitol - pharmacology ; Endothelins - pharmacology ; Enzyme Activation ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrolases ; Kinetics ; Phospholipases A - isolation & purification ; Phospholipases A - metabolism ; Phospholipases A2 ; Receptors, Endothelin - biosynthesis ; Receptors, Endothelin - drug effects ; Receptors, Endothelin - physiology ; Transfection</subject><ispartof>Biochemical and biophysical research communications, 1994-03, Vol.199 (2), p.992-997</ispartof><rights>1994 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c283t-2c0633f3b34a55e350ee74c51bd0cee33ab21b93cf0ec87f152ac2a80d6fab1b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X84713271$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4051294$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8135848$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schramek, H.</creatorcontrib><creatorcontrib>Wang, Y.</creatorcontrib><creatorcontrib>Konieczkowski, M.</creatorcontrib><creatorcontrib>Rose, P.M.</creatorcontrib><creatorcontrib>Sedor, J.R.</creatorcontrib><creatorcontrib>Dunn, M.J.</creatorcontrib><title>Endothelin-1 Stimulates Cytosolic Phospholipase A2 in Chinese Hamster Ovary Cells Stably Expressing the Human ETA or ETB Receptor Subtype</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The ability of endothelin-1 (ET-1) to activate cytosolic phospholipase A2 (cPLA2) has been studied in Chinese Hamster ovary (CHO) cells stably expressing either the human ETA (CHO/ETA) or the human ETB (CHO/ETB) receptor subtype. ET-1 dose-dependently increased a dithiothreitol-insensitive cPLA2 activity in both cell types. In CHO/ETA cells, BQ-123, an ETA-selective antagonist, completely blocked ET-1-induced cPLA2 stimulation. In CHO/ETB cells, the ET-1 response was mimicked by 4AlaET-1 which could be blocked partially by PD 145065, a nonselective antagonist of ETA and ETB. As expected, ET-1 stimulated PGE2 synthesis in CHO cells transfected with ET receptors. We conclude that ET-1 can stimulate cPLA2 via both the human ETA and ETB receptor subtype.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cytosol - enzymology</subject><subject>Dinoprostone - metabolism</subject><subject>Dithiothreitol - pharmacology</subject><subject>Endothelins - pharmacology</subject><subject>Enzyme Activation</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>Kinetics</subject><subject>Phospholipases A - isolation & purification</subject><subject>Phospholipases A - metabolism</subject><subject>Phospholipases A2</subject><subject>Receptors, Endothelin - biosynthesis</subject><subject>Receptors, Endothelin - drug effects</subject><subject>Receptors, Endothelin - physiology</subject><subject>Transfection</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE2P0zAQhi0EWsrClRuSD2hvKf5KmhxLVCjSSovYReJm2c6EGiVO8Dgr-hP417hqtTdO49E8fj1-CHnL2ZozVn2wNro1bxq15lJsnpEVZw0rBGfqOVmxTBSi4T9ekleIvxjjXFXNFbmquSxrVa_I313opnSAwYeC0_vkx2UwCZC2xzThNHhHvx4mnA_5OBsEuhXUB9oefIDc7c2ICSK9ezTxSFsYBswhxg5HuvszR0D04SfN-XS_jCbQ3cOWTjGXj_QbOJhTbu4Xm44zvCYvejMgvLnUa_L90-6h3Re3d5-_tNvbwolapkI4VknZSyuVKUuQJQPYKFdy2zEHIKWxgttGup6Bqzc9L4VxwtSsq3pjuZXX5OacO8fp9wKY9OjR5c1NgGlBvakUK4UqM7g-gy5OiBF6PUc_5n9qzvTJvT651yf3-uQ-X3h3SV7sCN0TfpGd5-8vc4PODH00wXl8wvKzXDQqY_UZg2zh0UPU6DwEB52P4JLuJv-_Df4B7lChIw</recordid><startdate>19940315</startdate><enddate>19940315</enddate><creator>Schramek, H.</creator><creator>Wang, Y.</creator><creator>Konieczkowski, M.</creator><creator>Rose, P.M.</creator><creator>Sedor, J.R.</creator><creator>Dunn, M.J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940315</creationdate><title>Endothelin-1 Stimulates Cytosolic Phospholipase A2 in Chinese Hamster Ovary Cells Stably Expressing the Human ETA or ETB Receptor Subtype</title><author>Schramek, H. ; Wang, Y. ; Konieczkowski, M. ; Rose, P.M. ; Sedor, J.R. ; Dunn, M.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c283t-2c0633f3b34a55e350ee74c51bd0cee33ab21b93cf0ec87f152ac2a80d6fab1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cytosol - enzymology</topic><topic>Dinoprostone - metabolism</topic><topic>Dithiothreitol - pharmacology</topic><topic>Endothelins - pharmacology</topic><topic>Enzyme Activation</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>Kinetics</topic><topic>Phospholipases A - isolation & purification</topic><topic>Phospholipases A - metabolism</topic><topic>Phospholipases A2</topic><topic>Receptors, Endothelin - biosynthesis</topic><topic>Receptors, Endothelin - drug effects</topic><topic>Receptors, Endothelin - physiology</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schramek, H.</creatorcontrib><creatorcontrib>Wang, Y.</creatorcontrib><creatorcontrib>Konieczkowski, M.</creatorcontrib><creatorcontrib>Rose, P.M.</creatorcontrib><creatorcontrib>Sedor, J.R.</creatorcontrib><creatorcontrib>Dunn, M.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schramek, H.</au><au>Wang, Y.</au><au>Konieczkowski, M.</au><au>Rose, P.M.</au><au>Sedor, J.R.</au><au>Dunn, M.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelin-1 Stimulates Cytosolic Phospholipase A2 in Chinese Hamster Ovary Cells Stably Expressing the Human ETA or ETB Receptor Subtype</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-03-15</date><risdate>1994</risdate><volume>199</volume><issue>2</issue><spage>992</spage><epage>997</epage><pages>992-997</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The ability of endothelin-1 (ET-1) to activate cytosolic phospholipase A2 (cPLA2) has been studied in Chinese Hamster ovary (CHO) cells stably expressing either the human ETA (CHO/ETA) or the human ETB (CHO/ETB) receptor subtype. ET-1 dose-dependently increased a dithiothreitol-insensitive cPLA2 activity in both cell types. In CHO/ETA cells, BQ-123, an ETA-selective antagonist, completely blocked ET-1-induced cPLA2 stimulation. In CHO/ETB cells, the ET-1 response was mimicked by 4AlaET-1 which could be blocked partially by PD 145065, a nonselective antagonist of ETA and ETB. As expected, ET-1 stimulated PGE2 synthesis in CHO cells transfected with ET receptors. We conclude that ET-1 can stimulate cPLA2 via both the human ETA and ETB receptor subtype.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8135848</pmid><doi>10.1006/bbrc.1994.1327</doi><tpages>6</tpages></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Blotting, Western CHO Cells Cricetinae Cytosol - enzymology Dinoprostone - metabolism Dithiothreitol - pharmacology Endothelins - pharmacology Enzyme Activation Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Humans Hydrolases Kinetics Phospholipases A - isolation & purification Phospholipases A - metabolism Phospholipases A2 Receptors, Endothelin - biosynthesis Receptors, Endothelin - drug effects Receptors, Endothelin - physiology Transfection |
| title | Endothelin-1 Stimulates Cytosolic Phospholipase A2 in Chinese Hamster Ovary Cells Stably Expressing the Human ETA or ETB Receptor Subtype |
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