Expression and regulation of basic fibroblast growth factor mRNA levels in mouse osteoblastic MC3T3-E1 cells
Basic fibroblast growth factor (bFGF) is a potent mitogen for bone cells and is a constituent of the bone matrix. We have found that osteoblastic MC3T3-E1 cells expressed bFGF mRNA transcript of 4.5 kilobases (kb). We examined factors that regulate the expression of bFGF mRNA and protein in MC3T3-E1...
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| Veröffentlicht in: | The Journal of biological chemistry 1994-03, Vol.269 (12), p.9392-9396 |
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| container_title | The Journal of biological chemistry |
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| creator | HURLEY, M. M ABREU, C GRONOWICZ, G KAWAGUCHI, H LORENZO, J |
| description | Basic fibroblast growth factor (bFGF) is a potent mitogen for bone cells and is a constituent of the bone matrix. We have
found that osteoblastic MC3T3-E1 cells expressed bFGF mRNA transcript of 4.5 kilobases (kb). We examined factors that regulate
the expression of bFGF mRNA and protein in MC3T3-E1 cells. Treatment of MC3T3-E1 cells with bFGF (10 nM) for 4-48 h induced
another 7-kb bFGF transcript at 4 h. Treatment of MC3T3-E1 cells with TGF beta (10 ng/ml) also induced the 7-kb transcript
of bFGF mRNA. In contrast, heparin, parathyroid hormone, and interleukin-1 had no effect on bFGF mRNA. Western blot analyses
revealed that MC3T3-E1 cells produced a 24-kDa bFGF protein, which was increased by TGF beta. Immunofluorescence showed that
bFGF protein was localized to the cytoplasm in serum-deprived MC3T3-E1 cells. Treatment of these cultures with medium containing
fetal calf serum or TGF beta caused increased cytoplasmic staining for bFGF and marked shape change. Furthermore, in the cells
treated with TGF beta there was both nuclear and cytoplasmic staining for the protein. These data demonstrate that bFGF mRNA
and protein are expressed in osteoblastic cells and are regulated by treatment with TGF beta and bFGF. Production of bFGF
may be important as an autocrine and paracrine mediator of bone cell replication, differentiation, and function. |
| doi_str_mv | 10.1016/s0021-9258(17)37121-1 |
| format | Article |
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found that osteoblastic MC3T3-E1 cells expressed bFGF mRNA transcript of 4.5 kilobases (kb). We examined factors that regulate
the expression of bFGF mRNA and protein in MC3T3-E1 cells. Treatment of MC3T3-E1 cells with bFGF (10 nM) for 4-48 h induced
another 7-kb bFGF transcript at 4 h. Treatment of MC3T3-E1 cells with TGF beta (10 ng/ml) also induced the 7-kb transcript
of bFGF mRNA. In contrast, heparin, parathyroid hormone, and interleukin-1 had no effect on bFGF mRNA. Western blot analyses
revealed that MC3T3-E1 cells produced a 24-kDa bFGF protein, which was increased by TGF beta. Immunofluorescence showed that
bFGF protein was localized to the cytoplasm in serum-deprived MC3T3-E1 cells. Treatment of these cultures with medium containing
fetal calf serum or TGF beta caused increased cytoplasmic staining for bFGF and marked shape change. Furthermore, in the cells
treated with TGF beta there was both nuclear and cytoplasmic staining for the protein. These data demonstrate that bFGF mRNA
and protein are expressed in osteoblastic cells and are regulated by treatment with TGF beta and bFGF. Production of bFGF
may be important as an autocrine and paracrine mediator of bone cell replication, differentiation, and function.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(17)37121-1</identifier><identifier>PMID: 8132679</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Cell Line ; Cytokines - pharmacology ; Fibroblast Growth Factor 2 - genetics ; Fibroblast Growth Factor 2 - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Mice ; Osteoblasts - metabolism ; Parathyroid Hormone - pharmacology ; Protein hormones. Growth factors. Cytokines ; Proteins ; RNA, Messenger - genetics ; Transforming Growth Factor beta - pharmacology</subject><ispartof>The Journal of biological chemistry, 1994-03, Vol.269 (12), p.9392-9396</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4721-296bcb40a265b483aa31675e3a70c23c1e4d92f8f6b3a0b2c35d8f9cdbecb8933</citedby><cites>FETCH-LOGICAL-c4721-296bcb40a265b483aa31675e3a70c23c1e4d92f8f6b3a0b2c35d8f9cdbecb8933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4111537$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8132679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HURLEY, M. M</creatorcontrib><creatorcontrib>ABREU, C</creatorcontrib><creatorcontrib>GRONOWICZ, G</creatorcontrib><creatorcontrib>KAWAGUCHI, H</creatorcontrib><creatorcontrib>LORENZO, J</creatorcontrib><title>Expression and regulation of basic fibroblast growth factor mRNA levels in mouse osteoblastic MC3T3-E1 cells</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Basic fibroblast growth factor (bFGF) is a potent mitogen for bone cells and is a constituent of the bone matrix. We have
found that osteoblastic MC3T3-E1 cells expressed bFGF mRNA transcript of 4.5 kilobases (kb). We examined factors that regulate
the expression of bFGF mRNA and protein in MC3T3-E1 cells. Treatment of MC3T3-E1 cells with bFGF (10 nM) for 4-48 h induced
another 7-kb bFGF transcript at 4 h. Treatment of MC3T3-E1 cells with TGF beta (10 ng/ml) also induced the 7-kb transcript
of bFGF mRNA. In contrast, heparin, parathyroid hormone, and interleukin-1 had no effect on bFGF mRNA. Western blot analyses
revealed that MC3T3-E1 cells produced a 24-kDa bFGF protein, which was increased by TGF beta. Immunofluorescence showed that
bFGF protein was localized to the cytoplasm in serum-deprived MC3T3-E1 cells. Treatment of these cultures with medium containing
fetal calf serum or TGF beta caused increased cytoplasmic staining for bFGF and marked shape change. Furthermore, in the cells
treated with TGF beta there was both nuclear and cytoplasmic staining for the protein. These data demonstrate that bFGF mRNA
and protein are expressed in osteoblastic cells and are regulated by treatment with TGF beta and bFGF. Production of bFGF
may be important as an autocrine and paracrine mediator of bone cell replication, differentiation, and function.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cytokines - pharmacology</subject><subject>Fibroblast Growth Factor 2 - genetics</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Mice</subject><subject>Osteoblasts - metabolism</subject><subject>Parathyroid Hormone - pharmacology</subject><subject>Protein hormones. Growth factors. Cytokines</subject><subject>Proteins</subject><subject>RNA, Messenger - genetics</subject><subject>Transforming Growth Factor beta - pharmacology</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo67j6ERYCiuihNZV0ks5xGcY_sCroCt5Ckk5mIt2d2aTH1W9v2hnmai5FUb-XerxC6ArIGyAg3hZCKDSK8u4VyNdMQu3gAVoB6VjDOPx4iFZn5DF6UspPUl-r4AJddMCokGqFhs3vffalxDRhM_U4--1hMPPSpoCtKdHhEG1OdjBlxtuc7ucdDsbNKePx6-drPPhffig4TnhMh-JxKrM_0lX6ac1uWbMB7PwwlKfoUTBD8c9O9RJ9f7e5XX9obr68_7i-vmlcK6thqoR1tiWGCm7bjhnDQEjumZHEUebAt72ioQvCMkMsdYz3XVCut97ZTjF2iV4e_93ndHfwZdZjLIsDM_nqUUvBlOCs_S8IgkuqgFeQH0GXUynZB73PcTT5jwail3Pob0vWeslag9T_zqGh6q5OCw529P1Zdcq_zl-c5qY4M4RsJhfLGWsB6nJZsedHbBe3u_uYvbYxuZ0fNRVKA9WKKcr-AsNSnig</recordid><startdate>19940325</startdate><enddate>19940325</enddate><creator>HURLEY, M. M</creator><creator>ABREU, C</creator><creator>GRONOWICZ, G</creator><creator>KAWAGUCHI, H</creator><creator>LORENZO, J</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>19940325</creationdate><title>Expression and regulation of basic fibroblast growth factor mRNA levels in mouse osteoblastic MC3T3-E1 cells</title><author>HURLEY, M. M ; ABREU, C ; GRONOWICZ, G ; KAWAGUCHI, H ; LORENZO, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4721-296bcb40a265b483aa31675e3a70c23c1e4d92f8f6b3a0b2c35d8f9cdbecb8933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cytokines - pharmacology</topic><topic>Fibroblast Growth Factor 2 - genetics</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Mice</topic><topic>Osteoblasts - metabolism</topic><topic>Parathyroid Hormone - pharmacology</topic><topic>Protein hormones. Growth factors. Cytokines</topic><topic>Proteins</topic><topic>RNA, Messenger - genetics</topic><topic>Transforming Growth Factor beta - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HURLEY, M. M</creatorcontrib><creatorcontrib>ABREU, C</creatorcontrib><creatorcontrib>GRONOWICZ, G</creatorcontrib><creatorcontrib>KAWAGUCHI, H</creatorcontrib><creatorcontrib>LORENZO, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HURLEY, M. M</au><au>ABREU, C</au><au>GRONOWICZ, G</au><au>KAWAGUCHI, H</au><au>LORENZO, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and regulation of basic fibroblast growth factor mRNA levels in mouse osteoblastic MC3T3-E1 cells</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-03-25</date><risdate>1994</risdate><volume>269</volume><issue>12</issue><spage>9392</spage><epage>9396</epage><pages>9392-9396</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Basic fibroblast growth factor (bFGF) is a potent mitogen for bone cells and is a constituent of the bone matrix. We have
found that osteoblastic MC3T3-E1 cells expressed bFGF mRNA transcript of 4.5 kilobases (kb). We examined factors that regulate
the expression of bFGF mRNA and protein in MC3T3-E1 cells. Treatment of MC3T3-E1 cells with bFGF (10 nM) for 4-48 h induced
another 7-kb bFGF transcript at 4 h. Treatment of MC3T3-E1 cells with TGF beta (10 ng/ml) also induced the 7-kb transcript
of bFGF mRNA. In contrast, heparin, parathyroid hormone, and interleukin-1 had no effect on bFGF mRNA. Western blot analyses
revealed that MC3T3-E1 cells produced a 24-kDa bFGF protein, which was increased by TGF beta. Immunofluorescence showed that
bFGF protein was localized to the cytoplasm in serum-deprived MC3T3-E1 cells. Treatment of these cultures with medium containing
fetal calf serum or TGF beta caused increased cytoplasmic staining for bFGF and marked shape change. Furthermore, in the cells
treated with TGF beta there was both nuclear and cytoplasmic staining for the protein. These data demonstrate that bFGF mRNA
and protein are expressed in osteoblastic cells and are regulated by treatment with TGF beta and bFGF. Production of bFGF
may be important as an autocrine and paracrine mediator of bone cell replication, differentiation, and function.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8132679</pmid><doi>10.1016/s0021-9258(17)37121-1</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1994-03, Vol.269 (12), p.9392-9396 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Line Cytokines - pharmacology Fibroblast Growth Factor 2 - genetics Fibroblast Growth Factor 2 - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression Mice Osteoblasts - metabolism Parathyroid Hormone - pharmacology Protein hormones. Growth factors. Cytokines Proteins RNA, Messenger - genetics Transforming Growth Factor beta - pharmacology |
| title | Expression and regulation of basic fibroblast growth factor mRNA levels in mouse osteoblastic MC3T3-E1 cells |
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