Comparisons of the growth in tissue culture of primary and metastatic melanoma
Tissue culture studies using monolayer cultures or semi-solid cultures under hypoxic or ambient oxygen conditions were carried out in 86 primary and 241 metastatic human melanomas. The frequencies with which growth occurred were similar for primary and metastatic tumours for each of the culture syst...
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| Veröffentlicht in: | Pathology 1985-01, Vol.17 (2), p.355-359 |
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| creator | Joyce, Robyn M. Vincent, P.C. Croaker, G.M. Young, G.A.R. |
| description | Tissue culture studies using monolayer cultures or semi-solid cultures under hypoxic or ambient oxygen conditions were carried out in 86 primary and 241 metastatic human melanomas. The frequencies with which growth occurred were similar for primary and metastatic tumours for each of the culture systems. In tumours which grew in hypoxic semi-solid culture, however, the plating efficiency (numbers of colonies per 5×105 cells plated) was significantly higher (26.1±2.8) for metastatic melanomas than for primaries (11.9±1.4).
Paired comparisons of the growth of individual tumours in hypoxic cultures with growth in ambient oxygen cultures confirmed earlier observations that growth was enhanced by hypoxia due to recruitment of additional colony-forming cells. The numbers of cells recruited under hypoxic conditions were significantly greater for metastatic tumours than for primaries. |
| doi_str_mv | 10.3109/00313028509063779 |
| format | Article |
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Paired comparisons of the growth of individual tumours in hypoxic cultures with growth in ambient oxygen cultures confirmed earlier observations that growth was enhanced by hypoxia due to recruitment of additional colony-forming cells. The numbers of cells recruited under hypoxic conditions were significantly greater for metastatic tumours than for primaries.</description><identifier>ISSN: 0031-3025</identifier><identifier>EISSN: 1465-3931</identifier><identifier>DOI: 10.3109/00313028509063779</identifier><identifier>PMID: 4047739</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Cell Division ; Cells, Cultured ; Culture Media ; Culture Techniques - methods ; Human melanoma ; Humans ; hypoxia ; Kinetics ; Melanoma - classification ; Melanoma - pathology ; metastatic ; Neoplasm Metastasis - pathology ; primary ; tissue culture ; tumour colony-forming units</subject><ispartof>Pathology, 1985-01, Vol.17 (2), p.355-359</ispartof><rights>1985 Royal College of Pathologists of Australasia</rights><rights>1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1985</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-cbc173c012b573769272521e34f66190c47bbe349f6ae71225e0dfd98563e1f43</citedby><cites>FETCH-LOGICAL-c400t-cbc173c012b573769272521e34f66190c47bbe349f6ae71225e0dfd98563e1f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/00313028509063779$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/00313028509063779$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,61221,61402</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4047739$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Joyce, Robyn M.</creatorcontrib><creatorcontrib>Vincent, P.C.</creatorcontrib><creatorcontrib>Croaker, G.M.</creatorcontrib><creatorcontrib>Young, G.A.R.</creatorcontrib><title>Comparisons of the growth in tissue culture of primary and metastatic melanoma</title><title>Pathology</title><addtitle>Pathology</addtitle><description>Tissue culture studies using monolayer cultures or semi-solid cultures under hypoxic or ambient oxygen conditions were carried out in 86 primary and 241 metastatic human melanomas. The frequencies with which growth occurred were similar for primary and metastatic tumours for each of the culture systems. In tumours which grew in hypoxic semi-solid culture, however, the plating efficiency (numbers of colonies per 5×105 cells plated) was significantly higher (26.1±2.8) for metastatic melanomas than for primaries (11.9±1.4).
Paired comparisons of the growth of individual tumours in hypoxic cultures with growth in ambient oxygen cultures confirmed earlier observations that growth was enhanced by hypoxia due to recruitment of additional colony-forming cells. The numbers of cells recruited under hypoxic conditions were significantly greater for metastatic tumours than for primaries.</description><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Culture Techniques - methods</subject><subject>Human melanoma</subject><subject>Humans</subject><subject>hypoxia</subject><subject>Kinetics</subject><subject>Melanoma - classification</subject><subject>Melanoma - pathology</subject><subject>metastatic</subject><subject>Neoplasm Metastasis - pathology</subject><subject>primary</subject><subject>tissue culture</subject><subject>tumour colony-forming units</subject><issn>0031-3025</issn><issn>1465-3931</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UE1r3DAQFaFhu9nkB-RQ8Kk3p6MPWxY9lSVNCkt7Sc5ClsdZLba1leSG_PtqWdNLIaeZ4X0w7xFyS-GOU1BfADjlwJoKFNRcSnVB1lTUVckVpx_I-oSXmVB9JFcxHgBANE2zIisBQkqu1uTn1o9HE1z0Uyx8X6Q9Fi_Bv6Z94aYiuRhnLOw8pDngCT8GN5rwVpipK0ZMJiaTnM3rYCY_mmty2Zsh4s0yN-T5-_3T9rHc_Xr4sf22K60ASKVtLZXcAmVtJbmsFZOsYhS56OuaKrBCtm2-VF8blJSxCqHrO9VUNUfaC74hn8--x-B_zxiTHl20OOQv0M9Ry5orwRjNRHom2uBjDNjrJYGmoE8d6v86zJpPi_ncjtj9UyylZfzrGXdT78No9miGtLcmoD74OUw5-LvuixpzP38cBh2tw8li5wLapDvv3lH_BZeSkGo</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Joyce, Robyn M.</creator><creator>Vincent, P.C.</creator><creator>Croaker, G.M.</creator><creator>Young, G.A.R.</creator><general>Elsevier B.V</general><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850101</creationdate><title>Comparisons of the growth in tissue culture of primary and metastatic melanoma</title><author>Joyce, Robyn M. ; Vincent, P.C. ; Croaker, G.M. ; Young, G.A.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-cbc173c012b573769272521e34f66190c47bbe349f6ae71225e0dfd98563e1f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Culture Techniques - methods</topic><topic>Human melanoma</topic><topic>Humans</topic><topic>hypoxia</topic><topic>Kinetics</topic><topic>Melanoma - classification</topic><topic>Melanoma - pathology</topic><topic>metastatic</topic><topic>Neoplasm Metastasis - pathology</topic><topic>primary</topic><topic>tissue culture</topic><topic>tumour colony-forming units</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Joyce, Robyn M.</creatorcontrib><creatorcontrib>Vincent, P.C.</creatorcontrib><creatorcontrib>Croaker, G.M.</creatorcontrib><creatorcontrib>Young, G.A.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Joyce, Robyn M.</au><au>Vincent, P.C.</au><au>Croaker, G.M.</au><au>Young, G.A.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparisons of the growth in tissue culture of primary and metastatic melanoma</atitle><jtitle>Pathology</jtitle><addtitle>Pathology</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>17</volume><issue>2</issue><spage>355</spage><epage>359</epage><pages>355-359</pages><issn>0031-3025</issn><eissn>1465-3931</eissn><abstract>Tissue culture studies using monolayer cultures or semi-solid cultures under hypoxic or ambient oxygen conditions were carried out in 86 primary and 241 metastatic human melanomas. The frequencies with which growth occurred were similar for primary and metastatic tumours for each of the culture systems. In tumours which grew in hypoxic semi-solid culture, however, the plating efficiency (numbers of colonies per 5×105 cells plated) was significantly higher (26.1±2.8) for metastatic melanomas than for primaries (11.9±1.4).
Paired comparisons of the growth of individual tumours in hypoxic cultures with growth in ambient oxygen cultures confirmed earlier observations that growth was enhanced by hypoxia due to recruitment of additional colony-forming cells. The numbers of cells recruited under hypoxic conditions were significantly greater for metastatic tumours than for primaries.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>4047739</pmid><doi>10.3109/00313028509063779</doi><tpages>5</tpages></addata></record> |
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| ispartof | Pathology, 1985-01, Vol.17 (2), p.355-359 |
| issn | 0031-3025 1465-3931 |
| language | eng |
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| source | MEDLINE; Taylor & Francis:Master (3349 titles); Alma/SFX Local Collection |
| subjects | Cell Division Cells, Cultured Culture Media Culture Techniques - methods Human melanoma Humans hypoxia Kinetics Melanoma - classification Melanoma - pathology metastatic Neoplasm Metastasis - pathology primary tissue culture tumour colony-forming units |
| title | Comparisons of the growth in tissue culture of primary and metastatic melanoma |
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