Quantitative Analysis of Alprazolam and Triazolam in Hemolysed Whole Blood and Liver Digest by GC/MS/NICI with Deuterated Internal Standards
This report describes sensitive and specific methods for the quantitation of alprazolam and triazolam in hemolysed whole blood and liver tissue. Samples of blood and enzyme-digested liver are extracted without pH adjustment with n-butyl chloride, after addition of deuterated internal standards and u...
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| Veröffentlicht in: | Journal of analytical toxicology 1994-01, Vol.18 (1), p.1-6 |
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| container_title | Journal of analytical toxicology |
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| creator | Cairns, Eric R. Dent, Barry R. Ouwerkerk, Joyce C. Porter, Lawrence J. |
| description | This report describes sensitive and specific methods for the quantitation of alprazolam and triazolam in hemolysed whole blood and liver tissue. Samples of blood and enzyme-digested liver are extracted without pH adjustment with n-butyl chloride, after addition of deuterated internal standards and urea. The evaporated extracts are reconstituted in acetonitrile for analysis by gas chromatography/mass spectrometry/negative ion chemical ionization (GC/MS/NICI). Fatty extracts may be cleaned up by partitioning between pentane and acetonitrile. Two ion pairs are monitored for each drug. Within-day coefficients of variation in the range 10–50 µg/L for blood are approximately 5%. Between-day coefficients of variation are less than 10%. The limit of quantitation (based on analysis of 0.2-mL blood samples) is 0.5 µg/L for triazolam and 4 µg/L for alprazolam. |
| doi_str_mv | 10.1093/jat/18.1.1 |
| format | Article |
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Samples of blood and enzyme-digested liver are extracted without pH adjustment with n-butyl chloride, after addition of deuterated internal standards and urea. The evaporated extracts are reconstituted in acetonitrile for analysis by gas chromatography/mass spectrometry/negative ion chemical ionization (GC/MS/NICI). Fatty extracts may be cleaned up by partitioning between pentane and acetonitrile. Two ion pairs are monitored for each drug. Within-day coefficients of variation in the range 10–50 µg/L for blood are approximately 5%. Between-day coefficients of variation are less than 10%. The limit of quantitation (based on analysis of 0.2-mL blood samples) is 0.5 µg/L for triazolam and 4 µg/L for alprazolam.</description><identifier>ISSN: 0146-4760</identifier><identifier>EISSN: 1945-2403</identifier><identifier>DOI: 10.1093/jat/18.1.1</identifier><identifier>PMID: 8127077</identifier><identifier>CODEN: JATOD3</identifier><language>eng</language><publisher>Niles, IL: Oxford University Press</publisher><subject>Alprazolam - analysis ; Alprazolam - blood ; Analysis ; Biological and medical sciences ; Deuterium ; Gas Chromatography-Mass Spectrometry - methods ; General pharmacology ; Humans ; Liver - chemistry ; Medical sciences ; Pharmacology. Drug treatments ; Reference Standards ; Reproducibility of Results ; Specimen Handling ; Triazolam - analysis ; Triazolam - blood</subject><ispartof>Journal of analytical toxicology, 1994-01, Vol.18 (1), p.1-6</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c328t-725863a9008c431525c7dc98b7ec7fdd0936f2580c86a20a384002224e7539203</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3903217$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8127077$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cairns, Eric R.</creatorcontrib><creatorcontrib>Dent, Barry R.</creatorcontrib><creatorcontrib>Ouwerkerk, Joyce C.</creatorcontrib><creatorcontrib>Porter, Lawrence J.</creatorcontrib><title>Quantitative Analysis of Alprazolam and Triazolam in Hemolysed Whole Blood and Liver Digest by GC/MS/NICI with Deuterated Internal Standards</title><title>Journal of analytical toxicology</title><addtitle>Journal of Analytical Toxicology</addtitle><description>This report describes sensitive and specific methods for the quantitation of alprazolam and triazolam in hemolysed whole blood and liver tissue. Samples of blood and enzyme-digested liver are extracted without pH adjustment with n-butyl chloride, after addition of deuterated internal standards and urea. The evaporated extracts are reconstituted in acetonitrile for analysis by gas chromatography/mass spectrometry/negative ion chemical ionization (GC/MS/NICI). Fatty extracts may be cleaned up by partitioning between pentane and acetonitrile. Two ion pairs are monitored for each drug. Within-day coefficients of variation in the range 10–50 µg/L for blood are approximately 5%. Between-day coefficients of variation are less than 10%. The limit of quantitation (based on analysis of 0.2-mL blood samples) is 0.5 µg/L for triazolam and 4 µg/L for alprazolam.</description><subject>Alprazolam - analysis</subject><subject>Alprazolam - blood</subject><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Deuterium</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Liver - chemistry</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Specimen Handling</subject><subject>Triazolam - analysis</subject><subject>Triazolam - blood</subject><issn>0146-4760</issn><issn>1945-2403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U9v0zAUAHALgUYZXLgj-YAQQkrrP4ntHEsKa1EHQhti4mK9Og7zSJNiO0D5DHxovDXqEU629X7v-dkPoaeUTCkp-ewG4oyqKZ3Se2hCy7zIWE74fTQhNBdZLgV5iB6FcEMIFUrwE3SiKJNEygn683GALroI0f2weN5Buw8u4L7B83bn4XffwhZDV-NL78aT6_DSbvsEbY0_X_etxa_bvq_v2DqV8XjhvtoQ8WaPz6rZ-cXs_apa4Z8uXuOFHaL1EFPqqku7dCG-iCkTfB0eowcNtME-GddT9Ontm8tqma0_nK2q-ToznKmYSVakV0BJiDI5pwUrjKxNqTbSGtnUdfoS0SRDjBLACHCVE8IYy60seMkIP0UvDnV3vv8-pE711gVj2xY62w9BS8FLzlXxX0iFVEIImuDLf8PUU16InMtEXx2o8X0I3jZ6590W_D6hO6fTODVVmurbus_GusNma-sjHeeX4s_HOAQDbeOhMy4cGS8JZ_SWZQfmQrS_jmHw37SQXBZ6efVFX4lFdb58V-mS_wVQTbVW</recordid><startdate>199401</startdate><enddate>199401</enddate><creator>Cairns, Eric R.</creator><creator>Dent, Barry R.</creator><creator>Ouwerkerk, Joyce C.</creator><creator>Porter, Lawrence J.</creator><general>Oxford University Press</general><general>Preston</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>199401</creationdate><title>Quantitative Analysis of Alprazolam and Triazolam in Hemolysed Whole Blood and Liver Digest by GC/MS/NICI with Deuterated Internal Standards</title><author>Cairns, Eric R. ; Dent, Barry R. ; Ouwerkerk, Joyce C. ; Porter, Lawrence J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c328t-725863a9008c431525c7dc98b7ec7fdd0936f2580c86a20a384002224e7539203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Alprazolam - analysis</topic><topic>Alprazolam - blood</topic><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Deuterium</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Liver - chemistry</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Specimen Handling</topic><topic>Triazolam - analysis</topic><topic>Triazolam - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cairns, Eric R.</creatorcontrib><creatorcontrib>Dent, Barry R.</creatorcontrib><creatorcontrib>Ouwerkerk, Joyce C.</creatorcontrib><creatorcontrib>Porter, Lawrence J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of analytical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cairns, Eric R.</au><au>Dent, Barry R.</au><au>Ouwerkerk, Joyce C.</au><au>Porter, Lawrence J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative Analysis of Alprazolam and Triazolam in Hemolysed Whole Blood and Liver Digest by GC/MS/NICI with Deuterated Internal Standards</atitle><jtitle>Journal of analytical toxicology</jtitle><addtitle>Journal of Analytical Toxicology</addtitle><date>1994-01</date><risdate>1994</risdate><volume>18</volume><issue>1</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>0146-4760</issn><eissn>1945-2403</eissn><coden>JATOD3</coden><abstract>This report describes sensitive and specific methods for the quantitation of alprazolam and triazolam in hemolysed whole blood and liver tissue. Samples of blood and enzyme-digested liver are extracted without pH adjustment with n-butyl chloride, after addition of deuterated internal standards and urea. The evaporated extracts are reconstituted in acetonitrile for analysis by gas chromatography/mass spectrometry/negative ion chemical ionization (GC/MS/NICI). Fatty extracts may be cleaned up by partitioning between pentane and acetonitrile. Two ion pairs are monitored for each drug. Within-day coefficients of variation in the range 10–50 µg/L for blood are approximately 5%. Between-day coefficients of variation are less than 10%. The limit of quantitation (based on analysis of 0.2-mL blood samples) is 0.5 µg/L for triazolam and 4 µg/L for alprazolam.</abstract><cop>Niles, IL</cop><pub>Oxford University Press</pub><pmid>8127077</pmid><doi>10.1093/jat/18.1.1</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0146-4760 |
| ispartof | Journal of analytical toxicology, 1994-01, Vol.18 (1), p.1-6 |
| issn | 0146-4760 1945-2403 |
| language | eng |
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| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; Alma/SFX Local Collection |
| subjects | Alprazolam - analysis Alprazolam - blood Analysis Biological and medical sciences Deuterium Gas Chromatography-Mass Spectrometry - methods General pharmacology Humans Liver - chemistry Medical sciences Pharmacology. Drug treatments Reference Standards Reproducibility of Results Specimen Handling Triazolam - analysis Triazolam - blood |
| title | Quantitative Analysis of Alprazolam and Triazolam in Hemolysed Whole Blood and Liver Digest by GC/MS/NICI with Deuterated Internal Standards |
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