A comparison of catecholamine-induced internalization of beta-adrenergic receptors and receptor-mediated endocytosis of epidermal growth factor in human astrocytoma cells. Inhibition by phenylarsine oxide
The ligand-induced internalization of beta-adrenergic receptors and the receptor-mediated internalization of epidermal growth factor were blocked, under similar conditions, by phenylarsine oxide (PAO) in human astrocytoma cells (1321N1). The inhibition was not prevented or reversed by monofunctional...
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| Veröffentlicht in: | The Journal of biological chemistry 1985-10, Vol.260 (23), p.12547-12553 |
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| creator | Hertel, C Coulter, S J Perkins, J P |
| description | The ligand-induced internalization of beta-adrenergic receptors and the receptor-mediated internalization of epidermal growth factor were blocked, under similar conditions, by phenylarsine oxide (PAO) in human astrocytoma cells (1321N1). The inhibition was not prevented or reversed by monofunctional sulfhydryl agents such as 2-mercaptoethanol or glutathione; however, the inhibitory action of PAO was blocked and reversed by bifunctional thiols such as 2,3-dimercaptoethanol or dithiothreitol. The results are consistent with the interaction of PAO with vicinal sulfhydryl groups to form a stabile ring structure. PAO did not prevent isoproterenol-induced uncoupling (desensitization) of beta-adrenergic receptors even though receptor internalization was completely blocked. The effects of PAO on receptor internalization could not be explained by any action of the trivalent arsenical to lower ATP levels. Ligand binding to both receptors was not detectably altered by PAO under conditions selective for inhibition for endocytosis. The results suggest a common mechanism for internalization of beta-adrenergic receptors and epidermal growth factor by a process that involves vicinal sulfhydryl groups. |
| doi_str_mv | 10.1016/S0021-9258(17)38906-8 |
| format | Article |
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Inhibition by phenylarsine oxide</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Hertel, C ; Coulter, S J ; Perkins, J P</creator><creatorcontrib>Hertel, C ; Coulter, S J ; Perkins, J P</creatorcontrib><description>The ligand-induced internalization of beta-adrenergic receptors and the receptor-mediated internalization of epidermal growth factor were blocked, under similar conditions, by phenylarsine oxide (PAO) in human astrocytoma cells (1321N1). The inhibition was not prevented or reversed by monofunctional sulfhydryl agents such as 2-mercaptoethanol or glutathione; however, the inhibitory action of PAO was blocked and reversed by bifunctional thiols such as 2,3-dimercaptoethanol or dithiothreitol. The results are consistent with the interaction of PAO with vicinal sulfhydryl groups to form a stabile ring structure. PAO did not prevent isoproterenol-induced uncoupling (desensitization) of beta-adrenergic receptors even though receptor internalization was completely blocked. The effects of PAO on receptor internalization could not be explained by any action of the trivalent arsenical to lower ATP levels. Ligand binding to both receptors was not detectably altered by PAO under conditions selective for inhibition for endocytosis. The results suggest a common mechanism for internalization of beta-adrenergic receptors and epidermal growth factor by a process that involves vicinal sulfhydryl groups.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)38906-8</identifier><identifier>PMID: 2995380</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Adenosine Triphosphate - metabolism ; Arsenicals - pharmacology ; Astrocytoma - metabolism ; Biological and medical sciences ; Catecholamines - pharmacology ; Cell Line ; Cell physiology ; Dithiothreitol - pharmacology ; Endocytosis ; Endocytosis - drug effects ; Epidermal Growth Factor - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; Iodocyanopindolol ; Isoproterenol - pharmacology ; Molecular and cellular biology ; Pindolol - analogs & derivatives ; Pindolol - metabolism ; Propanolamines - metabolism ; Receptors, Adrenergic, beta - drug effects ; Receptors, Adrenergic, beta - physiology</subject><ispartof>The Journal of biological chemistry, 1985-10, Vol.260 (23), p.12547-12553</ispartof><rights>1985 © 1985 ASBMB. 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Inhibition by phenylarsine oxide</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The ligand-induced internalization of beta-adrenergic receptors and the receptor-mediated internalization of epidermal growth factor were blocked, under similar conditions, by phenylarsine oxide (PAO) in human astrocytoma cells (1321N1). The inhibition was not prevented or reversed by monofunctional sulfhydryl agents such as 2-mercaptoethanol or glutathione; however, the inhibitory action of PAO was blocked and reversed by bifunctional thiols such as 2,3-dimercaptoethanol or dithiothreitol. The results are consistent with the interaction of PAO with vicinal sulfhydryl groups to form a stabile ring structure. PAO did not prevent isoproterenol-induced uncoupling (desensitization) of beta-adrenergic receptors even though receptor internalization was completely blocked. The effects of PAO on receptor internalization could not be explained by any action of the trivalent arsenical to lower ATP levels. Ligand binding to both receptors was not detectably altered by PAO under conditions selective for inhibition for endocytosis. The results suggest a common mechanism for internalization of beta-adrenergic receptors and epidermal growth factor by a process that involves vicinal sulfhydryl groups.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Arsenicals - pharmacology</subject><subject>Astrocytoma - metabolism</subject><subject>Biological and medical sciences</subject><subject>Catecholamines - pharmacology</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Dithiothreitol - pharmacology</subject><subject>Endocytosis</subject><subject>Endocytosis - drug effects</subject><subject>Epidermal Growth Factor - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Iodocyanopindolol</subject><subject>Isoproterenol - pharmacology</subject><subject>Molecular and cellular biology</subject><subject>Pindolol - analogs & derivatives</subject><subject>Pindolol - metabolism</subject><subject>Propanolamines - metabolism</subject><subject>Receptors, Adrenergic, beta - drug effects</subject><subject>Receptors, Adrenergic, beta - physiology</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQhSMEKqXwCJW8QAgWKXZ8HTsrVFX8VKrEApDYWRN7cmOU2MH2bbk8Iw-F748uS7wZWfOdmaM5VXXJ6BWjrH37hdKG1V0j1Gsm33DV0bZWj6pzRhWvuWDfH1fnJ-Rp9SylH7S8VcfOqrOm6wRX9Lz6c01MmBeILgVPwkAMZDRjmGB2Hmvn7cagJc5njB4m9xuyO4A9ZqjBRvQY186QiAaXHGIi4O3pV89oXRlpCXobzDaH5NJOjouzGGeYyDqGhzySAUzhyyYybmbwBFKOe8EMxOA0pSty60fXu72BfkuWEf12gpiKURJ-lXHPqycDTAlfHOtF9e3D-683n-q7zx9vb67vaiM4y3XHWgTeD0q2ojdWqb4ZjIChBcuFkLRvO76yaJltZatYJ4aVlUJKEM0g0a74RfXqMHeJ4ecGU9azSzuP4DFskpYtV1IqUUBxAE0MKUUc9BLdDHGrGdW7FPU-Rb2LSDOp9ylqVXSXxwWbvhzwpDrGVvovj31IBqYhgjcunTAlGqoE-4eNbj0-uIi6d8GMOOumpbrhmjViJQv27oBhudm9w6iTcehL7kVisrbB_cfvX88Sy90</recordid><startdate>19851015</startdate><enddate>19851015</enddate><creator>Hertel, C</creator><creator>Coulter, S J</creator><creator>Perkins, J P</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19851015</creationdate><title>A comparison of catecholamine-induced internalization of beta-adrenergic receptors and receptor-mediated endocytosis of epidermal growth factor in human astrocytoma cells. Inhibition by phenylarsine oxide</title><author>Hertel, C ; Coulter, S J ; Perkins, J P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c531t-916ea3bf8765bcd88b2fc5af6ad35570b6934ded1d6768195f4d7577a52f7ed43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Arsenicals - pharmacology</topic><topic>Astrocytoma - metabolism</topic><topic>Biological and medical sciences</topic><topic>Catecholamines - pharmacology</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>Dithiothreitol - pharmacology</topic><topic>Endocytosis</topic><topic>Endocytosis - drug effects</topic><topic>Epidermal Growth Factor - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Iodocyanopindolol</topic><topic>Isoproterenol - pharmacology</topic><topic>Molecular and cellular biology</topic><topic>Pindolol - analogs & derivatives</topic><topic>Pindolol - metabolism</topic><topic>Propanolamines - metabolism</topic><topic>Receptors, Adrenergic, beta - drug effects</topic><topic>Receptors, Adrenergic, beta - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hertel, C</creatorcontrib><creatorcontrib>Coulter, S J</creatorcontrib><creatorcontrib>Perkins, J P</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hertel, C</au><au>Coulter, S J</au><au>Perkins, J P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of catecholamine-induced internalization of beta-adrenergic receptors and receptor-mediated endocytosis of epidermal growth factor in human astrocytoma cells. Inhibition by phenylarsine oxide</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1985-10-15</date><risdate>1985</risdate><volume>260</volume><issue>23</issue><spage>12547</spage><epage>12553</epage><pages>12547-12553</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>The ligand-induced internalization of beta-adrenergic receptors and the receptor-mediated internalization of epidermal growth factor were blocked, under similar conditions, by phenylarsine oxide (PAO) in human astrocytoma cells (1321N1). The inhibition was not prevented or reversed by monofunctional sulfhydryl agents such as 2-mercaptoethanol or glutathione; however, the inhibitory action of PAO was blocked and reversed by bifunctional thiols such as 2,3-dimercaptoethanol or dithiothreitol. The results are consistent with the interaction of PAO with vicinal sulfhydryl groups to form a stabile ring structure. PAO did not prevent isoproterenol-induced uncoupling (desensitization) of beta-adrenergic receptors even though receptor internalization was completely blocked. The effects of PAO on receptor internalization could not be explained by any action of the trivalent arsenical to lower ATP levels. Ligand binding to both receptors was not detectably altered by PAO under conditions selective for inhibition for endocytosis. The results suggest a common mechanism for internalization of beta-adrenergic receptors and epidermal growth factor by a process that involves vicinal sulfhydryl groups.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>2995380</pmid><doi>10.1016/S0021-9258(17)38906-8</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1985-10, Vol.260 (23), p.12547-12553 |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Adenosine Triphosphate - metabolism Arsenicals - pharmacology Astrocytoma - metabolism Biological and medical sciences Catecholamines - pharmacology Cell Line Cell physiology Dithiothreitol - pharmacology Endocytosis Endocytosis - drug effects Epidermal Growth Factor - metabolism Fundamental and applied biological sciences. Psychology Humans Iodocyanopindolol Isoproterenol - pharmacology Molecular and cellular biology Pindolol - analogs & derivatives Pindolol - metabolism Propanolamines - metabolism Receptors, Adrenergic, beta - drug effects Receptors, Adrenergic, beta - physiology |
| title | A comparison of catecholamine-induced internalization of beta-adrenergic receptors and receptor-mediated endocytosis of epidermal growth factor in human astrocytoma cells. Inhibition by phenylarsine oxide |
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