Mineralization in vitro of matrix formed by osteoblasts isolated by collagenase digestion

Osteoblasts from calvaria of 18-day-old fetal Sprague-Dawley rats were isolated using a dissecting procedure followed by collagenase digestion. Freshly isolated or previously frozen cells were cultured for up to 4 weeks in a Dulbecco's modified Eagle's medium supplemented with 10% fetal ca...

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Veröffentlicht in:	Differentiation (London) 1985, Vol.29 (2), p.160-168
Hauptverfasser:	Nefussi, Jean-Raphaël, Boy-Lefevre, M.L., Boulekbache, Habib, Forest, Nadine
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - metabolism Animals Biological and medical sciences Bone Matrix - metabolism Calcium Phosphates - metabolism Cell Separation Cells, Cultured Collagen - analysis Embryology: invertebrates and vertebrates. Teratology Experimental organogenesis Fibroblasts Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Glycerophosphates - pharmacology Microbial Collagenase - metabolism Microscopy, Electron Microscopy, Electron, Scanning Organogenesis. Physiological fonctions Osteoblasts - enzymology Osteoblasts - metabolism Osteogenesis Rats Rats, Inbred Strains Space life sciences
Online-Zugang:	Volltext
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