Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae

The identification of a sorbitol-induced sorbitol dehydrogenase (SDH) activity from Saccharomyces cerevisiae is described. The SDH1 structural gene was isolated from a λgt11 yeast genomic library using an antibody to a 40-kDa protein induced in yeast cells growing in medium containing sorbitol. The...

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Veröffentlicht in:	Gene 1994, Vol.140 (1), p.121-126
Hauptverfasser:	Sarthy, Aparna V., Schopp, Cynthia, Idler, Kenneth B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Antibody Base Sequence Biological and medical sciences Cloning, Molecular DNA, Fungal Fundamental and applied biological sciences. Psychology genbank/l11039 Genes, Fungal Growth, nutrition, metabolism, transports, enzymes. Molecular biology L-Iditol 2-Dehydrogenase - biosynthesis L-Iditol 2-Dehydrogenase - genetics L-iditol dehydrogenase Microbiology Molecular Sequence Data Mycology nucleotide sequences Plasmids promoter regions recombinant DNA Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics sdh1 gene Sequence Analysis, DNA Sequence Homology, Amino Acid sheep liver sorbitol dehydrogenase structural genes λgt11 yeast genomic library
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