Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae
The identification of a sorbitol-induced sorbitol dehydrogenase (SDH) activity from Saccharomyces cerevisiae is described. The SDH1 structural gene was isolated from a λgt11 yeast genomic library using an antibody to a 40-kDa protein induced in yeast cells growing in medium containing sorbitol. The...
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| Veröffentlicht in: | Gene 1994, Vol.140 (1), p.121-126 |
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| creator | Sarthy, Aparna V. Schopp, Cynthia Idler, Kenneth B. |
| description | The identification of a sorbitol-induced sorbitol dehydrogenase (SDH) activity from
Saccharomyces cerevisiae is described. The
SDH1 structural gene was isolated from a λgt11 yeast genomic library using an antibody to a 40-kDa protein induced in yeast cells growing in medium containing sorbitol. The gene encodes a 357-amino-acid (aa) protein deduced from the nucleotide sequence. Comparison of the aa sequence of the yeast SDH1 with that of sheep liver SDH reveals a 63% overall similarity. Yeast transformants containing the cloned gene carried on a multicopy plasmid express high levels of SDH1 only when grown on sorbitol, suggesting that the cloned gene contains both regulatory and coding sequences. |
| doi_str_mv | 10.1016/0378-1119(94)90741-2 |
| format | Article |
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Saccharomyces cerevisiae is described. The
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Saccharomyces cerevisiae is described. The
SDH1 structural gene was isolated from a λgt11 yeast genomic library using an antibody to a 40-kDa protein induced in yeast cells growing in medium containing sorbitol. The gene encodes a 357-amino-acid (aa) protein deduced from the nucleotide sequence. Comparison of the aa sequence of the yeast SDH1 with that of sheep liver SDH reveals a 63% overall similarity. Yeast transformants containing the cloned gene carried on a multicopy plasmid express high levels of SDH1 only when grown on sorbitol, suggesting that the cloned gene contains both regulatory and coding sequences.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Antibody</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA, Fungal</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/l11039</subject><subject>Genes, Fungal</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>L-Iditol 2-Dehydrogenase - biosynthesis</subject><subject>L-Iditol 2-Dehydrogenase - genetics</subject><subject>L-iditol dehydrogenase</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mycology</subject><subject>nucleotide sequences</subject><subject>Plasmids</subject><subject>promoter regions</subject><subject>recombinant DNA</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>sdh1 gene</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>sheep liver sorbitol dehydrogenase</subject><subject>structural genes</subject><subject>λgt11 yeast genomic library</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EKkvhG4DwASE4hPpfYvtSCa1aQKrUQ9uz5diTXaPELna20n77OuxqjzAXj_R-Mxq_h9B7Sr5RQrsLwqVqKKX6ixZfNZGCNuwFWlEldUMIVy_R6oS8Rm9K-U1qtS07Q2eKspYztULjekwxxA220eMCf3YQHWAPM-QpRDuHFHEa8LwFvIEIuMrJL3xJuQ9zGiu73fucqmoL4CGnCd9Z57a2dnsHBTvI8BRKsPAWvRrsWODd8T1HD9dX9-ufzc3tj1_r7zeNa4mcG6s8Y6RXROqOKO-7lkppB-alFlJo3g9Wdd5p7anVjNNOtb1mDFrpQHHG-Tn6fNj7mFP9UZnNFIqDcbQR0q4Y2XElWHXgfyDttCC6YxUUB9DlVEqGwTzmMNm8N5SYJQ2zWG0Wq40W5m8aZhn7cNy_6yfwp6Gj_VX_dNRtcXYcso0ulBMmiJBKLmd-PGCDTcZuckUe7hihnFChNCeiEpcHAqqtTwGyKS4sUfqQwc3Gp_DvS58BTPSvcw</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Sarthy, Aparna V.</creator><creator>Schopp, Cynthia</creator><creator>Idler, Kenneth B.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae</title><author>Sarthy, Aparna V. ; Schopp, Cynthia ; Idler, Kenneth B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-a8d220b8079608dd65177af2d7947493bfa86dc99d1a9231685b922e57ce83233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Antibody</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA, Fungal</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/l11039</topic><topic>Genes, Fungal</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>L-Iditol 2-Dehydrogenase - biosynthesis</topic><topic>L-Iditol 2-Dehydrogenase - genetics</topic><topic>L-iditol dehydrogenase</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mycology</topic><topic>nucleotide sequences</topic><topic>Plasmids</topic><topic>promoter regions</topic><topic>recombinant DNA</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>sdh1 gene</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>sheep liver sorbitol dehydrogenase</topic><topic>structural genes</topic><topic>λgt11 yeast genomic library</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarthy, Aparna V.</creatorcontrib><creatorcontrib>Schopp, Cynthia</creatorcontrib><creatorcontrib>Idler, Kenneth B.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarthy, Aparna V.</au><au>Schopp, Cynthia</au><au>Idler, Kenneth B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1994</date><risdate>1994</risdate><volume>140</volume><issue>1</issue><spage>121</spage><epage>126</epage><pages>121-126</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>The identification of a sorbitol-induced sorbitol dehydrogenase (SDH) activity from
Saccharomyces cerevisiae is described. The
SDH1 structural gene was isolated from a λgt11 yeast genomic library using an antibody to a 40-kDa protein induced in yeast cells growing in medium containing sorbitol. The gene encodes a 357-amino-acid (aa) protein deduced from the nucleotide sequence. Comparison of the aa sequence of the yeast SDH1 with that of sheep liver SDH reveals a 63% overall similarity. Yeast transformants containing the cloned gene carried on a multicopy plasmid express high levels of SDH1 only when grown on sorbitol, suggesting that the cloned gene contains both regulatory and coding sequences.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8125328</pmid><doi>10.1016/0378-1119(94)90741-2</doi><tpages>6</tpages></addata></record> |
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| ispartof | Gene, 1994, Vol.140 (1), p.121-126 |
| issn | 0378-1119 1879-0038 |
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| subjects | Amino Acid Sequence amino acid sequences Antibody Base Sequence Biological and medical sciences Cloning, Molecular DNA, Fungal Fundamental and applied biological sciences. Psychology genbank/l11039 Genes, Fungal Growth, nutrition, metabolism, transports, enzymes. Molecular biology L-Iditol 2-Dehydrogenase - biosynthesis L-Iditol 2-Dehydrogenase - genetics L-iditol dehydrogenase Microbiology Molecular Sequence Data Mycology nucleotide sequences Plasmids promoter regions recombinant DNA Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics sdh1 gene Sequence Analysis, DNA Sequence Homology, Amino Acid sheep liver sorbitol dehydrogenase structural genes λgt11 yeast genomic library |
| title | Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae |
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