Cell Adhesion Activity of a 30-kDa Major Secreted Protein from Human Bladder Carcinoma Cells
Human bladder carcinoma cell line EJ-1 secreted a protein of 30 kDa as a major component. This protein was purified from the serum-free conditioned medium of EJ-1. Its N-terminal amino acid sequence was identical to that of fibroblast-derived endothelial cell growth factor (f-ECGF), but there were d...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1994-02, Vol.198 (3), p.1046-1053 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 198 |
| creator | Akaogi, K. Okabe, Y. Funahashi, K. Yoshitake, Y. Nishikawa, K. Yasumitsu, H. Umeda, M. Miyazaki, K. |
| description | Human bladder carcinoma cell line EJ-1 secreted a protein of 30 kDa as a major component. This protein was purified from the serum-free conditioned medium of EJ-1. Its N-terminal amino acid sequence was identical to that of fibroblast-derived endothelial cell growth factor (f-ECGF), but there were differences in their amino acid compositions and isoelectric points. The 30-kDa protein showed no effect on the growth of bovine capillary endothelial cells, but it promoted the attachment and spreading of rat liver cell line BRL and human umbilical vein endothelial cell line ECV-304. These results suggest that the 30-kDa protein, tentatively named "tumor-derived adhesion factor (TAF)", may be related with the aberrant cell adhesion of cancer cells. |
| doi_str_mv | 10.1006/bbrc.1994.1149 |
| format | Article |
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This protein was purified from the serum-free conditioned medium of EJ-1. Its N-terminal amino acid sequence was identical to that of fibroblast-derived endothelial cell growth factor (f-ECGF), but there were differences in their amino acid compositions and isoelectric points. The 30-kDa protein showed no effect on the growth of bovine capillary endothelial cells, but it promoted the attachment and spreading of rat liver cell line BRL and human umbilical vein endothelial cell line ECV-304. These results suggest that the 30-kDa protein, tentatively named "tumor-derived adhesion factor (TAF)", may be related with the aberrant cell adhesion of cancer cells.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1994.1149</identifier><identifier>PMID: 8117260</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Biological and medical sciences ; Capillaries ; Cattle ; Cell Adhesion ; Cell Division - drug effects ; Cell Line ; Chromatography, High Pressure Liquid ; Chromatography, Ion Exchange ; Endothelial Growth Factors - chemistry ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Fibroblast Growth Factor 2 - pharmacology ; Humans ; Liver ; Medical sciences ; Molecular Sequence Data ; Molecular Weight ; Neoplasm Proteins - chemistry ; Neoplasm Proteins - isolation & purification ; Neoplasm Proteins - pharmacology ; Nephrology. Urinary tract diseases ; Rats ; Sequence Homology, Amino Acid ; Tumor Cells, Cultured ; Tumors of the urinary system ; Umbilical Veins ; Urinary Bladder Neoplasms ; Urinary tract. Prostate gland</subject><ispartof>Biochemical and biophysical research communications, 1994-02, Vol.198 (3), p.1046-1053</ispartof><rights>1994 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-396f8012c59d35fa3284ef52f2eb3ddf3d58a0798d06ac675aa2064baf26a7ff3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X84711491$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3949962$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8117260$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akaogi, K.</creatorcontrib><creatorcontrib>Okabe, Y.</creatorcontrib><creatorcontrib>Funahashi, K.</creatorcontrib><creatorcontrib>Yoshitake, Y.</creatorcontrib><creatorcontrib>Nishikawa, K.</creatorcontrib><creatorcontrib>Yasumitsu, H.</creatorcontrib><creatorcontrib>Umeda, M.</creatorcontrib><creatorcontrib>Miyazaki, K.</creatorcontrib><title>Cell Adhesion Activity of a 30-kDa Major Secreted Protein from Human Bladder Carcinoma Cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Human bladder carcinoma cell line EJ-1 secreted a protein of 30 kDa as a major component. This protein was purified from the serum-free conditioned medium of EJ-1. Its N-terminal amino acid sequence was identical to that of fibroblast-derived endothelial cell growth factor (f-ECGF), but there were differences in their amino acid compositions and isoelectric points. The 30-kDa protein showed no effect on the growth of bovine capillary endothelial cells, but it promoted the attachment and spreading of rat liver cell line BRL and human umbilical vein endothelial cell line ECV-304. These results suggest that the 30-kDa protein, tentatively named "tumor-derived adhesion factor (TAF)", may be related with the aberrant cell adhesion of cancer cells.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Capillaries</subject><subject>Cattle</subject><subject>Cell Adhesion</subject><subject>Cell Division - drug effects</subject><subject>Cell Line</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Ion Exchange</subject><subject>Endothelial Growth Factors - chemistry</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Humans</subject><subject>Liver</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Neoplasm Proteins - chemistry</subject><subject>Neoplasm Proteins - isolation & purification</subject><subject>Neoplasm Proteins - pharmacology</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Rats</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors of the urinary system</subject><subject>Umbilical Veins</subject><subject>Urinary Bladder Neoplasms</subject><subject>Urinary tract. Prostate gland</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1r3DAQhkVJSDebXnsr6BB680Yftmwdt9umG0hIIAn0UBBjaUSV2lYieQP597XZJbfOZQ7vMy_DQ8hnzlacMXXRtsmuuNblivNSfyALzjQrBGflEVmwiSiE5r8-ktOcnxibGKVPyEnDeS0UW5DfG-w6unZ_MIc40LUdw2sY32j0FKhkxd_vQG_gKSZ6jzbhiI7epThiGKhPsafbXQ8D_daBc5joBpINQ-yBzrX5jBx76DJ-Ouwlebz88bDZFte3P6826-vClrIcC6mVbxgXttJOVh6kaEr0lfACW-mcl65qgNW6cUyBVXUFIJgqW_BCQe29XJKv-97nFF92mEfTh2ynD2DAuMumVrIRcpolWe1Bm2LOCb15TqGH9GY4M7NOM-s0s04z65wOvhyad22P7h0_-Jvy80MO2ULnEww25HdM6lJrJSas2WM4WXgNmEy2AQeLLiS0o3Ex_O-Df4L-j4U</recordid><startdate>19940215</startdate><enddate>19940215</enddate><creator>Akaogi, K.</creator><creator>Okabe, Y.</creator><creator>Funahashi, K.</creator><creator>Yoshitake, Y.</creator><creator>Nishikawa, K.</creator><creator>Yasumitsu, H.</creator><creator>Umeda, M.</creator><creator>Miyazaki, K.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940215</creationdate><title>Cell Adhesion Activity of a 30-kDa Major Secreted Protein from Human Bladder Carcinoma Cells</title><author>Akaogi, K. ; Okabe, Y. ; Funahashi, K. ; Yoshitake, Y. ; Nishikawa, K. ; Yasumitsu, H. ; Umeda, M. ; Miyazaki, K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-396f8012c59d35fa3284ef52f2eb3ddf3d58a0798d06ac675aa2064baf26a7ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Capillaries</topic><topic>Cattle</topic><topic>Cell Adhesion</topic><topic>Cell Division - drug effects</topic><topic>Cell Line</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Ion Exchange</topic><topic>Endothelial Growth Factors - chemistry</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Humans</topic><topic>Liver</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>Neoplasm Proteins - chemistry</topic><topic>Neoplasm Proteins - isolation & purification</topic><topic>Neoplasm Proteins - pharmacology</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Rats</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors of the urinary system</topic><topic>Umbilical Veins</topic><topic>Urinary Bladder Neoplasms</topic><topic>Urinary tract. Prostate gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akaogi, K.</creatorcontrib><creatorcontrib>Okabe, Y.</creatorcontrib><creatorcontrib>Funahashi, K.</creatorcontrib><creatorcontrib>Yoshitake, Y.</creatorcontrib><creatorcontrib>Nishikawa, K.</creatorcontrib><creatorcontrib>Yasumitsu, H.</creatorcontrib><creatorcontrib>Umeda, M.</creatorcontrib><creatorcontrib>Miyazaki, K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akaogi, K.</au><au>Okabe, Y.</au><au>Funahashi, K.</au><au>Yoshitake, Y.</au><au>Nishikawa, K.</au><au>Yasumitsu, H.</au><au>Umeda, M.</au><au>Miyazaki, K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell Adhesion Activity of a 30-kDa Major Secreted Protein from Human Bladder Carcinoma Cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1994-02-15</date><risdate>1994</risdate><volume>198</volume><issue>3</issue><spage>1046</spage><epage>1053</epage><pages>1046-1053</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Human bladder carcinoma cell line EJ-1 secreted a protein of 30 kDa as a major component. This protein was purified from the serum-free conditioned medium of EJ-1. Its N-terminal amino acid sequence was identical to that of fibroblast-derived endothelial cell growth factor (f-ECGF), but there were differences in their amino acid compositions and isoelectric points. The 30-kDa protein showed no effect on the growth of bovine capillary endothelial cells, but it promoted the attachment and spreading of rat liver cell line BRL and human umbilical vein endothelial cell line ECV-304. These results suggest that the 30-kDa protein, tentatively named "tumor-derived adhesion factor (TAF)", may be related with the aberrant cell adhesion of cancer cells.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8117260</pmid><doi>10.1006/bbrc.1994.1149</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1994-02, Vol.198 (3), p.1046-1053 |
| issn | 0006-291X 1090-2104 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Biological and medical sciences Capillaries Cattle Cell Adhesion Cell Division - drug effects Cell Line Chromatography, High Pressure Liquid Chromatography, Ion Exchange Endothelial Growth Factors - chemistry Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Fibroblast Growth Factor 2 - pharmacology Humans Liver Medical sciences Molecular Sequence Data Molecular Weight Neoplasm Proteins - chemistry Neoplasm Proteins - isolation & purification Neoplasm Proteins - pharmacology Nephrology. Urinary tract diseases Rats Sequence Homology, Amino Acid Tumor Cells, Cultured Tumors of the urinary system Umbilical Veins Urinary Bladder Neoplasms Urinary tract. Prostate gland |
| title | Cell Adhesion Activity of a 30-kDa Major Secreted Protein from Human Bladder Carcinoma Cells |
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