Extrathymic Development and Function of Human T-Lymphocytes from Bone Marrow Cells in Vitro
To enrich low-density human bone marrow (BM) cells for putative progenitors of T-lymphocytes, CD7 + CD3 - cells were sorted (purity was estimated at >99.9%) and cultured under limiting dilution conditions with irradiated allogeneic stimulator cells, interleukin (IL) 2, and PHA. Clonal populations...
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| Veröffentlicht in: | Cellular immunology 1994-03, Vol.154 (1), p.25-42 |
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| creator | Adibzadeh, M. Bühring, H.J. Daikeler, T. Siegels-Hübenthal, P. Owsianowsky, M. Schenk, A. Rehbein, A. Schaudt, K. Schlotz, E. Pohla, H. Pawelec, G. |
| description | To enrich low-density human bone marrow (BM) cells for putative progenitors of T-lymphocytes, CD7
+ CD3
- cells were sorted (purity was estimated at >99.9%) and cultured under limiting dilution conditions with irradiated allogeneic stimulator cells, interleukin (IL) 2, and PHA. Clonal populations were available for analysis from Day 25 onward. By this time, all clones (
n = 54) expressed CD3 and α/β-T cell receptor (TCR2). Fifty percent of the clones were CD4
+ and 50% were CD8
+, with no double positives, whereas almost all clones obtained under identical conditions from peripheral blood (PB) cells were CD4
+. All clones were capable of autocrine proliferation, which was blocked by CD25 or CD71 mAb. Most or all clones tested (
n = 15) responded to IL 4 and IL 7 as well as IL 2, but not to IL 3 or GM-CSF and only two responded to IL 9. Most clones accumulated mRNA for GM-CSF, IL 2, IL 3, IL 4, IL 5 and also IL 9, but 6 of 11 were negative for IFN-γ mRNA, and all were negative for IL 6 mRNA. Sixty-two percent of CD4
+ and 85% of CD8
+ clones (total 70% of all clones) mediated lectin-dependent cell lysis; but whereas 35% of CD4
+ and 65% of CD8
+ clones (total 46% of all clones) lysed K562 natural killer (NK)-susceptible targets, only 24% of CD4
+ and 5% of CD8
+ clones (total 17% of all clones) killed lymphokine-activated killer (LAK)-susceptible Daudi cells. Only three clones lysed allogeneic LCL targets and none lysed autologous targets. Furthermore, none of the clones proliferated when stimulated by autologous cells, neither did they suppress proliferative responses of autologous cells. These results suggest that CD3
- cells from the bone marrow can acquire functional cytotoxic and proliferative programs extrathymically during
in vitro culture with IL 2, mitogens and allogeneic cells, but do not manifest autoreactivity in the three test systems, cytotoxicity, suppression, or autocrine proliferation. |
| doi_str_mv | 10.1006/cimm.1994.1054 |
| format | Article |
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+ CD3
- cells were sorted (purity was estimated at >99.9%) and cultured under limiting dilution conditions with irradiated allogeneic stimulator cells, interleukin (IL) 2, and PHA. Clonal populations were available for analysis from Day 25 onward. By this time, all clones (
n = 54) expressed CD3 and α/β-T cell receptor (TCR2). Fifty percent of the clones were CD4
+ and 50% were CD8
+, with no double positives, whereas almost all clones obtained under identical conditions from peripheral blood (PB) cells were CD4
+. All clones were capable of autocrine proliferation, which was blocked by CD25 or CD71 mAb. Most or all clones tested (
n = 15) responded to IL 4 and IL 7 as well as IL 2, but not to IL 3 or GM-CSF and only two responded to IL 9. Most clones accumulated mRNA for GM-CSF, IL 2, IL 3, IL 4, IL 5 and also IL 9, but 6 of 11 were negative for IFN-γ mRNA, and all were negative for IL 6 mRNA. Sixty-two percent of CD4
+ and 85% of CD8
+ clones (total 70% of all clones) mediated lectin-dependent cell lysis; but whereas 35% of CD4
+ and 65% of CD8
+ clones (total 46% of all clones) lysed K562 natural killer (NK)-susceptible targets, only 24% of CD4
+ and 5% of CD8
+ clones (total 17% of all clones) killed lymphokine-activated killer (LAK)-susceptible Daudi cells. Only three clones lysed allogeneic LCL targets and none lysed autologous targets. Furthermore, none of the clones proliferated when stimulated by autologous cells, neither did they suppress proliferative responses of autologous cells. These results suggest that CD3
- cells from the bone marrow can acquire functional cytotoxic and proliferative programs extrathymically during
in vitro culture with IL 2, mitogens and allogeneic cells, but do not manifest autoreactivity in the three test systems, cytotoxicity, suppression, or autocrine proliferation.</description><identifier>ISSN: 0008-8749</identifier><identifier>EISSN: 1090-2163</identifier><identifier>DOI: 10.1006/cimm.1994.1054</identifier><identifier>PMID: 8118889</identifier><identifier>CODEN: CLIMB8</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Antigens, Differentiation, T-Lymphocyte - genetics ; Antigens, Differentiation, T-Lymphocyte - metabolism ; Base Sequence ; Biological and medical sciences ; Bone Marrow - immunology ; Bone Marrow Cells ; Cell Differentiation ; Cell Separation ; Clone Cells - cytology ; Clone Cells - drug effects ; Clone Cells - immunology ; Cytokines - genetics ; Cytokines - metabolism ; Cytokines - pharmacology ; Cytotoxicity, Immunologic ; DNA Primers - genetics ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - drug effects ; Hematopoietic Stem Cells - immunology ; Humans ; Immunobiology ; In Vitro Techniques ; Lymphocyte Activation ; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation ; Mitogens - pharmacology ; Molecular Sequence Data ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; T-Lymphocytes - cytology ; T-Lymphocytes - drug effects ; T-Lymphocytes - immunology</subject><ispartof>Cellular immunology, 1994-03, Vol.154 (1), p.25-42</ispartof><rights>1994 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-ad170ab7a2491f9193f2508a3aa20ebdbd65a9f821a3897ea803c93cc3e6ec433</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/cimm.1994.1054$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3981160$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8118889$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adibzadeh, M.</creatorcontrib><creatorcontrib>Bühring, H.J.</creatorcontrib><creatorcontrib>Daikeler, T.</creatorcontrib><creatorcontrib>Siegels-Hübenthal, P.</creatorcontrib><creatorcontrib>Owsianowsky, M.</creatorcontrib><creatorcontrib>Schenk, A.</creatorcontrib><creatorcontrib>Rehbein, A.</creatorcontrib><creatorcontrib>Schaudt, K.</creatorcontrib><creatorcontrib>Schlotz, E.</creatorcontrib><creatorcontrib>Pohla, H.</creatorcontrib><creatorcontrib>Pawelec, G.</creatorcontrib><title>Extrathymic Development and Function of Human T-Lymphocytes from Bone Marrow Cells in Vitro</title><title>Cellular immunology</title><addtitle>Cell Immunol</addtitle><description>To enrich low-density human bone marrow (BM) cells for putative progenitors of T-lymphocytes, CD7
+ CD3
- cells were sorted (purity was estimated at >99.9%) and cultured under limiting dilution conditions with irradiated allogeneic stimulator cells, interleukin (IL) 2, and PHA. Clonal populations were available for analysis from Day 25 onward. By this time, all clones (
n = 54) expressed CD3 and α/β-T cell receptor (TCR2). Fifty percent of the clones were CD4
+ and 50% were CD8
+, with no double positives, whereas almost all clones obtained under identical conditions from peripheral blood (PB) cells were CD4
+. All clones were capable of autocrine proliferation, which was blocked by CD25 or CD71 mAb. Most or all clones tested (
n = 15) responded to IL 4 and IL 7 as well as IL 2, but not to IL 3 or GM-CSF and only two responded to IL 9. Most clones accumulated mRNA for GM-CSF, IL 2, IL 3, IL 4, IL 5 and also IL 9, but 6 of 11 were negative for IFN-γ mRNA, and all were negative for IL 6 mRNA. Sixty-two percent of CD4
+ and 85% of CD8
+ clones (total 70% of all clones) mediated lectin-dependent cell lysis; but whereas 35% of CD4
+ and 65% of CD8
+ clones (total 46% of all clones) lysed K562 natural killer (NK)-susceptible targets, only 24% of CD4
+ and 5% of CD8
+ clones (total 17% of all clones) killed lymphokine-activated killer (LAK)-susceptible Daudi cells. Only three clones lysed allogeneic LCL targets and none lysed autologous targets. Furthermore, none of the clones proliferated when stimulated by autologous cells, neither did they suppress proliferative responses of autologous cells. These results suggest that CD3
- cells from the bone marrow can acquire functional cytotoxic and proliferative programs extrathymically during
in vitro culture with IL 2, mitogens and allogeneic cells, but do not manifest autoreactivity in the three test systems, cytotoxicity, suppression, or autocrine proliferation.</description><subject>Antigens, Differentiation, T-Lymphocyte - genetics</subject><subject>Antigens, Differentiation, T-Lymphocyte - metabolism</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow - immunology</subject><subject>Bone Marrow Cells</subject><subject>Cell Differentiation</subject><subject>Cell Separation</subject><subject>Clone Cells - cytology</subject><subject>Clone Cells - drug effects</subject><subject>Clone Cells - immunology</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Cytokines - pharmacology</subject><subject>Cytotoxicity, Immunologic</subject><subject>DNA Primers - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - drug effects</subject><subject>Hematopoietic Stem Cells - immunology</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>In Vitro Techniques</subject><subject>Lymphocyte Activation</subject><subject>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</subject><subject>Mitogens - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - immunology</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFPGzEQRq2qFaTQKzckH6reNrXXm137SAOUSkFcgAsHa-KdFUZrO9he2vz7OkrEreppNPrejD49Qs44m3PG2u_GOjfnSjVlXTQfyIwzxaqat-IjmTHGZCW7Rh2Tzym9MMZ5o9gROZKcSynVjDxd_ckR8vPWWUMv8Q3HsHHoMwXf0-vJm2yDp2GgN5MDT--r1dZtnoPZZkx0iMHRH8EjvYUYw2-6xHFM1Hr6aHMMp-TTAGPCL4d5Qh6ur-6XN9Xq7uev5cWqMkKpXEHPOwbrDupG8UFxJYZ6wSQIgJrhul_37QLUIGsOQqoOQTJhlDBGYIumEeKEfNv_3cTwOmHK2tlkShXwGKaku1aUY17_F-StWohatQWc70ETQ0oRB72J1kHcas70Trveadc77XqnvRycHz5Pa4f9O37wXPKvhxySgXGI4I1N75hQBWxZweQew6LrzWLUyVj0Bnsb0WTdB_uvBn8B_cWeIA</recordid><startdate>19940301</startdate><enddate>19940301</enddate><creator>Adibzadeh, M.</creator><creator>Bühring, H.J.</creator><creator>Daikeler, T.</creator><creator>Siegels-Hübenthal, P.</creator><creator>Owsianowsky, M.</creator><creator>Schenk, A.</creator><creator>Rehbein, A.</creator><creator>Schaudt, K.</creator><creator>Schlotz, E.</creator><creator>Pohla, H.</creator><creator>Pawelec, G.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19940301</creationdate><title>Extrathymic Development and Function of Human T-Lymphocytes from Bone Marrow Cells in Vitro</title><author>Adibzadeh, M. ; Bühring, H.J. ; Daikeler, T. ; Siegels-Hübenthal, P. ; Owsianowsky, M. ; Schenk, A. ; Rehbein, A. ; Schaudt, K. ; Schlotz, E. ; Pohla, H. ; Pawelec, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-ad170ab7a2491f9193f2508a3aa20ebdbd65a9f821a3897ea803c93cc3e6ec433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antigens, Differentiation, T-Lymphocyte - genetics</topic><topic>Antigens, Differentiation, T-Lymphocyte - metabolism</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow - immunology</topic><topic>Bone Marrow Cells</topic><topic>Cell Differentiation</topic><topic>Cell Separation</topic><topic>Clone Cells - cytology</topic><topic>Clone Cells - drug effects</topic><topic>Clone Cells - immunology</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Cytokines - pharmacology</topic><topic>Cytotoxicity, Immunologic</topic><topic>DNA Primers - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - drug effects</topic><topic>Hematopoietic Stem Cells - immunology</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>In Vitro Techniques</topic><topic>Lymphocyte Activation</topic><topic>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</topic><topic>Mitogens - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>T-Lymphocytes - cytology</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adibzadeh, M.</creatorcontrib><creatorcontrib>Bühring, H.J.</creatorcontrib><creatorcontrib>Daikeler, T.</creatorcontrib><creatorcontrib>Siegels-Hübenthal, P.</creatorcontrib><creatorcontrib>Owsianowsky, M.</creatorcontrib><creatorcontrib>Schenk, A.</creatorcontrib><creatorcontrib>Rehbein, A.</creatorcontrib><creatorcontrib>Schaudt, K.</creatorcontrib><creatorcontrib>Schlotz, E.</creatorcontrib><creatorcontrib>Pohla, H.</creatorcontrib><creatorcontrib>Pawelec, G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adibzadeh, M.</au><au>Bühring, H.J.</au><au>Daikeler, T.</au><au>Siegels-Hübenthal, P.</au><au>Owsianowsky, M.</au><au>Schenk, A.</au><au>Rehbein, A.</au><au>Schaudt, K.</au><au>Schlotz, E.</au><au>Pohla, H.</au><au>Pawelec, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extrathymic Development and Function of Human T-Lymphocytes from Bone Marrow Cells in Vitro</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>1994-03-01</date><risdate>1994</risdate><volume>154</volume><issue>1</issue><spage>25</spage><epage>42</epage><pages>25-42</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><coden>CLIMB8</coden><abstract>To enrich low-density human bone marrow (BM) cells for putative progenitors of T-lymphocytes, CD7
+ CD3
- cells were sorted (purity was estimated at >99.9%) and cultured under limiting dilution conditions with irradiated allogeneic stimulator cells, interleukin (IL) 2, and PHA. Clonal populations were available for analysis from Day 25 onward. By this time, all clones (
n = 54) expressed CD3 and α/β-T cell receptor (TCR2). Fifty percent of the clones were CD4
+ and 50% were CD8
+, with no double positives, whereas almost all clones obtained under identical conditions from peripheral blood (PB) cells were CD4
+. All clones were capable of autocrine proliferation, which was blocked by CD25 or CD71 mAb. Most or all clones tested (
n = 15) responded to IL 4 and IL 7 as well as IL 2, but not to IL 3 or GM-CSF and only two responded to IL 9. Most clones accumulated mRNA for GM-CSF, IL 2, IL 3, IL 4, IL 5 and also IL 9, but 6 of 11 were negative for IFN-γ mRNA, and all were negative for IL 6 mRNA. Sixty-two percent of CD4
+ and 85% of CD8
+ clones (total 70% of all clones) mediated lectin-dependent cell lysis; but whereas 35% of CD4
+ and 65% of CD8
+ clones (total 46% of all clones) lysed K562 natural killer (NK)-susceptible targets, only 24% of CD4
+ and 5% of CD8
+ clones (total 17% of all clones) killed lymphokine-activated killer (LAK)-susceptible Daudi cells. Only three clones lysed allogeneic LCL targets and none lysed autologous targets. Furthermore, none of the clones proliferated when stimulated by autologous cells, neither did they suppress proliferative responses of autologous cells. These results suggest that CD3
- cells from the bone marrow can acquire functional cytotoxic and proliferative programs extrathymically during
in vitro culture with IL 2, mitogens and allogeneic cells, but do not manifest autoreactivity in the three test systems, cytotoxicity, suppression, or autocrine proliferation.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8118889</pmid><doi>10.1006/cimm.1994.1054</doi><tpages>18</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-8749 |
| ispartof | Cellular immunology, 1994-03, Vol.154 (1), p.25-42 |
| issn | 0008-8749 1090-2163 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76382112 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Antigens, Differentiation, T-Lymphocyte - genetics Antigens, Differentiation, T-Lymphocyte - metabolism Base Sequence Biological and medical sciences Bone Marrow - immunology Bone Marrow Cells Cell Differentiation Cell Separation Clone Cells - cytology Clone Cells - drug effects Clone Cells - immunology Cytokines - genetics Cytokines - metabolism Cytokines - pharmacology Cytotoxicity, Immunologic DNA Primers - genetics Fundamental and applied biological sciences. Psychology Fundamental immunology Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - drug effects Hematopoietic Stem Cells - immunology Humans Immunobiology In Vitro Techniques Lymphocyte Activation Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation Mitogens - pharmacology Molecular Sequence Data RNA, Messenger - genetics RNA, Messenger - metabolism T-Lymphocytes - cytology T-Lymphocytes - drug effects T-Lymphocytes - immunology |
| title | Extrathymic Development and Function of Human T-Lymphocytes from Bone Marrow Cells in Vitro |
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