Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information
The epithelial HBL-100 cell line was established in vitro from milk of an apparently healthy woman [2, 3]. It exhibits characteristics of transformation from the very beginning and evolves during in vitro maintenance, until becoming tumorigenic in nude mice. This immortal cell line represents a usef...
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| Veröffentlicht in: | Experimental cell research 1985-01, Vol.160 (1), p.83-94 |
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| container_title | Experimental cell research |
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| creator | de Fromentel, C.Caron Nardeux, P.C. Soussi, T. Lavialle, C. Estrade, S. Carloni, G. Chandrasekaran, K. Cassingena, R. |
| description | The epithelial HBL-100 cell line was established in vitro from milk of an apparently healthy woman [2, 3]. It exhibits characteristics of transformation from the very beginning and evolves during in vitro maintenance, until becoming tumorigenic in nude mice. This immortal cell line represents a useful model for studying the progression of human epithelial cells toward malignancy. In the course of our investigations we detected a 94K protein in HBL-100 cells obtained from four different sources. This protein is shown to be indistinguishable from the SV40 large T-antigen on the basis of:
1.
1, Recognition by polyclonal and different monoclonal antibodies.
2.
2, Partial peptide map analysis.
3.
3, Specific binding capacity to the SV40 DNA origin of replication.
The presence of a tandemly integrated SV40 genome is demonstrated by Southern blotting. Successful rescue of SV40 DNA by fusion with permissive COS-7, but not CV-1 cells, indicates that the SV40 T-antigen from HBL-100 cells is defective in a function(s) essential to the replication of the viral DNA. The possible origin of the SV40 genetic information that we have detected in HBL-100 cells and the implications of this finding on studies involving this cell line are discussed. |
| doi_str_mv | 10.1016/0014-4827(85)90238-1 |
| format | Article |
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1.
1, Recognition by polyclonal and different monoclonal antibodies.
2.
2, Partial peptide map analysis.
3.
3, Specific binding capacity to the SV40 DNA origin of replication.
The presence of a tandemly integrated SV40 genome is demonstrated by Southern blotting. Successful rescue of SV40 DNA by fusion with permissive COS-7, but not CV-1 cells, indicates that the SV40 T-antigen from HBL-100 cells is defective in a function(s) essential to the replication of the viral DNA. The possible origin of the SV40 genetic information that we have detected in HBL-100 cells and the implications of this finding on studies involving this cell line are discussed.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(85)90238-1</identifier><identifier>PMID: 2995096</identifier><identifier>CODEN: ECREAL</identifier><language>eng</language><publisher>Orlando, FL: Elsevier Inc</publisher><subject>Adenocarcinoma - etiology ; Adult ; Animals ; Antigens, Polyomavirus Transforming ; Antigens, Viral, Tumor - analysis ; Antigens, Viral, Tumor - genetics ; Antigens, Viral, Tumor - metabolism ; Biological and medical sciences ; Breast - cytology ; Breast - microbiology ; Cell Line ; Cell Transformation, Neoplastic ; Cell Transformation, Viral ; DNA Replication ; DNA, Viral - metabolism ; Epithelial Cells ; Epithelium - microbiology ; Female ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; Humans ; Mice ; Mice, Nude ; Microbiology ; Milk, Human ; Neoplasm Transplantation ; Nucleic Acid Hybridization ; Oncogene Proteins, Viral - analysis ; Oncogene Proteins, Viral - genetics ; Oncogene Proteins, Viral - metabolism ; Recombination, Genetic ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Simian virus 40 - genetics ; Virology</subject><ispartof>Experimental cell research, 1985-01, Vol.160 (1), p.83-94</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-4170b5fe6e1f4beeab287d325a4c4ea228ff4dbb6246b9f5422c55c2f9a46fad3</citedby><cites>FETCH-LOGICAL-c417t-4170b5fe6e1f4beeab287d325a4c4ea228ff4dbb6246b9f5422c55c2f9a46fad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(85)90238-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8713082$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2995096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Fromentel, C.Caron</creatorcontrib><creatorcontrib>Nardeux, P.C.</creatorcontrib><creatorcontrib>Soussi, T.</creatorcontrib><creatorcontrib>Lavialle, C.</creatorcontrib><creatorcontrib>Estrade, S.</creatorcontrib><creatorcontrib>Carloni, G.</creatorcontrib><creatorcontrib>Chandrasekaran, K.</creatorcontrib><creatorcontrib>Cassingena, R.</creatorcontrib><title>Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>The epithelial HBL-100 cell line was established in vitro from milk of an apparently healthy woman [2, 3]. It exhibits characteristics of transformation from the very beginning and evolves during in vitro maintenance, until becoming tumorigenic in nude mice. This immortal cell line represents a useful model for studying the progression of human epithelial cells toward malignancy. In the course of our investigations we detected a 94K protein in HBL-100 cells obtained from four different sources. This protein is shown to be indistinguishable from the SV40 large T-antigen on the basis of:
1.
1, Recognition by polyclonal and different monoclonal antibodies.
2.
2, Partial peptide map analysis.
3.
3, Specific binding capacity to the SV40 DNA origin of replication.
The presence of a tandemly integrated SV40 genome is demonstrated by Southern blotting. Successful rescue of SV40 DNA by fusion with permissive COS-7, but not CV-1 cells, indicates that the SV40 T-antigen from HBL-100 cells is defective in a function(s) essential to the replication of the viral DNA. The possible origin of the SV40 genetic information that we have detected in HBL-100 cells and the implications of this finding on studies involving this cell line are discussed.</description><subject>Adenocarcinoma - etiology</subject><subject>Adult</subject><subject>Animals</subject><subject>Antigens, Polyomavirus Transforming</subject><subject>Antigens, Viral, Tumor - analysis</subject><subject>Antigens, Viral, Tumor - genetics</subject><subject>Antigens, Viral, Tumor - metabolism</subject><subject>Biological and medical sciences</subject><subject>Breast - cytology</subject><subject>Breast - microbiology</subject><subject>Cell Line</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cell Transformation, Viral</subject><subject>DNA Replication</subject><subject>DNA, Viral - metabolism</subject><subject>Epithelial Cells</subject><subject>Epithelium - microbiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Microbiology</subject><subject>Milk, Human</subject><subject>Neoplasm Transplantation</subject><subject>Nucleic Acid Hybridization</subject><subject>Oncogene Proteins, Viral - analysis</subject><subject>Oncogene Proteins, Viral - genetics</subject><subject>Oncogene Proteins, Viral - metabolism</subject><subject>Recombination, Genetic</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Simian virus 40 - genetics</subject><subject>Virology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1EVZbCNwDJB4TgELAdO3EuSFCVttJKHPhztSbOmDU4dmpni_bbk2VXeyyXmcP7zWjmPUJecPaOM968Z4zLSmrRvtHqbcdErSv-iKw461glpBCPyeqEPCFPS_nFGNOaN-fkXHSdYl2zIulq8vMGg4dAbz6tK84YtRgCDT4iHTD7exyoy2mkow-_aXIUIoVpgoxxDju6QQjzZkf_pHERNpD7tM2Ffv0hGf2JEWdvqY8u5RFmn-IzcuYgFHx-7Bfk--erb5c31frL9e3lx3VlJW_naimsVw4b5E72iNAL3Q61UCCtRBBCOyeHvm-EbPrOqeVdq5QVrgPZOBjqC_L6sHfK6W6LZTajL_vHIGLaFtM2tWZd3f4X5LKpFW_1AsoDaHMqJaMzU_Yj5J3hzOwDMXu3zd5to5X5F4jhy9jL4_5tP-JwGjomsOivjjoUC8FliNaXE6ZbXjMtFuzDAcPFtHuP2RTrMVocfEY7myH5h-_4Cxy3pug</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>de Fromentel, C.Caron</creator><creator>Nardeux, P.C.</creator><creator>Soussi, T.</creator><creator>Lavialle, C.</creator><creator>Estrade, S.</creator><creator>Carloni, G.</creator><creator>Chandrasekaran, K.</creator><creator>Cassingena, R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19850101</creationdate><title>Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information</title><author>de Fromentel, C.Caron ; Nardeux, P.C. ; Soussi, T. ; Lavialle, C. ; Estrade, S. ; Carloni, G. ; Chandrasekaran, K. ; Cassingena, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-4170b5fe6e1f4beeab287d325a4c4ea228ff4dbb6246b9f5422c55c2f9a46fad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Adenocarcinoma - etiology</topic><topic>Adult</topic><topic>Animals</topic><topic>Antigens, Polyomavirus Transforming</topic><topic>Antigens, Viral, Tumor - analysis</topic><topic>Antigens, Viral, Tumor - genetics</topic><topic>Antigens, Viral, Tumor - metabolism</topic><topic>Biological and medical sciences</topic><topic>Breast - cytology</topic><topic>Breast - microbiology</topic><topic>Cell Line</topic><topic>Cell Transformation, Neoplastic</topic><topic>Cell Transformation, Viral</topic><topic>DNA Replication</topic><topic>DNA, Viral - metabolism</topic><topic>Epithelial Cells</topic><topic>Epithelium - microbiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Microbiology</topic><topic>Milk, Human</topic><topic>Neoplasm Transplantation</topic><topic>Nucleic Acid Hybridization</topic><topic>Oncogene Proteins, Viral - analysis</topic><topic>Oncogene Proteins, Viral - genetics</topic><topic>Oncogene Proteins, Viral - metabolism</topic><topic>Recombination, Genetic</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Simian virus 40 - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Fromentel, C.Caron</creatorcontrib><creatorcontrib>Nardeux, P.C.</creatorcontrib><creatorcontrib>Soussi, T.</creatorcontrib><creatorcontrib>Lavialle, C.</creatorcontrib><creatorcontrib>Estrade, S.</creatorcontrib><creatorcontrib>Carloni, G.</creatorcontrib><creatorcontrib>Chandrasekaran, K.</creatorcontrib><creatorcontrib>Cassingena, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Fromentel, C.Caron</au><au>Nardeux, P.C.</au><au>Soussi, T.</au><au>Lavialle, C.</au><au>Estrade, S.</au><au>Carloni, G.</au><au>Chandrasekaran, K.</au><au>Cassingena, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>160</volume><issue>1</issue><spage>83</spage><epage>94</epage><pages>83-94</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>The epithelial HBL-100 cell line was established in vitro from milk of an apparently healthy woman [2, 3]. It exhibits characteristics of transformation from the very beginning and evolves during in vitro maintenance, until becoming tumorigenic in nude mice. This immortal cell line represents a useful model for studying the progression of human epithelial cells toward malignancy. In the course of our investigations we detected a 94K protein in HBL-100 cells obtained from four different sources. This protein is shown to be indistinguishable from the SV40 large T-antigen on the basis of:
1.
1, Recognition by polyclonal and different monoclonal antibodies.
2.
2, Partial peptide map analysis.
3.
3, Specific binding capacity to the SV40 DNA origin of replication.
The presence of a tandemly integrated SV40 genome is demonstrated by Southern blotting. Successful rescue of SV40 DNA by fusion with permissive COS-7, but not CV-1 cells, indicates that the SV40 T-antigen from HBL-100 cells is defective in a function(s) essential to the replication of the viral DNA. The possible origin of the SV40 genetic information that we have detected in HBL-100 cells and the implications of this finding on studies involving this cell line are discussed.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>2995096</pmid><doi>10.1016/0014-4827(85)90238-1</doi><tpages>12</tpages></addata></record> |
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| ispartof | Experimental cell research, 1985-01, Vol.160 (1), p.83-94 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Adenocarcinoma - etiology Adult Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor - analysis Antigens, Viral, Tumor - genetics Antigens, Viral, Tumor - metabolism Biological and medical sciences Breast - cytology Breast - microbiology Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral DNA Replication DNA, Viral - metabolism Epithelial Cells Epithelium - microbiology Female Fundamental and applied biological sciences. Psychology Genes, Viral Humans Mice Mice, Nude Microbiology Milk, Human Neoplasm Transplantation Nucleic Acid Hybridization Oncogene Proteins, Viral - analysis Oncogene Proteins, Viral - genetics Oncogene Proteins, Viral - metabolism Recombination, Genetic Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Simian virus 40 - genetics Virology |
| title | Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information |
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