Controlled-expression shuttle vector for pseudomonads based on the trpIBA genes of Pseudomonas putida

A cloning vector pPS7 (8.5 kb) for Pseudomonas was constructed from pBR322 and the Pseudomonas cryptic low-copy-number pMK1 plasmid. The vector confers resistance to kanamycin (Km) and tetracycline (Tc), contains the par locus of Pseudomonas plasmid pMT2 and a mob site. The new vector was used for c...

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Veröffentlicht in:	Gene 1994, Vol.140 (1), p.63-65
Hauptverfasser:	Olekhnovich, Igor N., Fomichev, Yuri K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic engineering Genetic technics Genetic Vectors Kanamycin Resistance - genetics low-copy-number plasmid Methods. Procedures. Technologies Promoter Regions, Genetic Pseudomonas putida Pseudomonas putida - genetics Recombinant DNA Tetracycline Resistance - genetics Tryptophan Synthase - genetics Vectors (cloning, transfer, expression). Insertion sequences and transposons
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