An improved cytochemical method for the evaluation of eosinophil colonies in soft-agar cultures from human blood or bone marrow

An improved cytochemical method for the evaluation of eosinophil colonies in soft-agar cultures from human blood or bone marrow

A simple peroxidase staining method specific for eosinophils using 4-chloro-1-naphthol as substrate and 1,2,4-benzene triol as selective inhibitor is applied to the analysis of eosinophil colonies grown in soft-agar cultures form human blood or bone marrow. Eosinophil colonies appear strongly staine...

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Veröffentlicht in:Acta histochemica 1985, Vol.76 (2), p.219-223
1. Verfasser: Dufer, Jean
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Agar
Bone Marrow - pathology
Cells, Cultured
Eosinophils - cytology
Eosinophils - enzymology
Humans
Peroxidases - blood
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container_title Acta histochemica
container_volume 76
creator Dufer, Jean
description A simple peroxidase staining method specific for eosinophils using 4-chloro-1-naphthol as substrate and 1,2,4-benzene triol as selective inhibitor is applied to the analysis of eosinophil colonies grown in soft-agar cultures form human blood or bone marrow. Eosinophil colonies appear strongly stained in black whereas other types of colonies (neutrophils and macrophages) remain colourless. The proposed method is easy to be performed, quick, reliable, may be associated without major problems to many other cytochemical stains and promises to be of value in hemopoiesis studies.
doi_str_mv 10.1016/S0065-1281(85)80062-3
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subjects Adult
Agar
Bone Marrow - pathology
Cells, Cultured
Eosinophils - cytology
Eosinophils - enzymology
Humans
Peroxidases - blood
title An improved cytochemical method for the evaluation of eosinophil colonies in soft-agar cultures from human blood or bone marrow
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