Expression of Growth Hormone Receptor Gene in Rat Hypothalamus
Growth hormone receptor (GHR) mRNA‐expressing cells in the hypothalamus were observed using hybridization histochemistry in adult male rats. Digoxigenin‐labeled cRNA corresponding to the extracellular part of rat GHR was used as a probe. Northern blotting analysis of hypothalamic total RNA from adul...
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| Veröffentlicht in: | Journal of neuroendocrinology 1993-12, Vol.5 (6), p.691-696 |
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| container_title | Journal of neuroendocrinology |
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| creator | Minami, Shiro Kamegai, Jun Hasegawa, Osamu Sugihara, Hitoshi Okada, Kenmei Wakabayashi, Ichiji |
| description | Growth hormone receptor (GHR) mRNA‐expressing cells in the hypothalamus were observed using hybridization histochemistry in adult male rats. Digoxigenin‐labeled cRNA corresponding to the extracellular part of rat GHR was used as a probe. Northern blotting analysis of hypothalamic total RNA from adult male rats revealed that the 4.5 kilobase (kb) transcript of the GHR gene corresponding to the GHR messenger RNA (mRNA) predominated over the 1.2 kb transcript corresponding to GH‐binding protein mRNA. GHR mRNA‐containing cells were observed in the arcuate nucleus (ARC), the periventricular nucleus (PeV), ventrolateral region of the ventromedial nucleus, the paraventricular nucleus and the supraoptic nucleus. To further understand the significance of the GHR gene expression in the hypothalamus, the effect of in vivo manipulation of GH on the somatostatin (SS) gene expression in the ARC and PeV, and the GRF gene expression in the ARC was observed among adult male rats using in situ hybridization histochemistry. Ten days after hypophysectomy, the SS mRNA level in the ARC as well as PeV was significantly lower than that in the respective nuclei of sham‐operated control rats, while the GRF mRNA level in the ARC was significantly higher than that in the ARC of control animals. Subcutaneous injection of recombinant human GH (0.33 mg) to hypophysectomized rats every 12 h for 5 days restored the SS mRNA level in the ARC and PeV, and reduced the GRF mRNA level in the ARC to that of control animals. The data suggest that GH directly acts on the hypothalamic PeV and ARC, and alters the gene expression of SS and GRF. |
| doi_str_mv | 10.1111/j.1365-2826.1993.tb00541.x |
| format | Article |
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Digoxigenin‐labeled cRNA corresponding to the extracellular part of rat GHR was used as a probe. Northern blotting analysis of hypothalamic total RNA from adult male rats revealed that the 4.5 kilobase (kb) transcript of the GHR gene corresponding to the GHR messenger RNA (mRNA) predominated over the 1.2 kb transcript corresponding to GH‐binding protein mRNA. GHR mRNA‐containing cells were observed in the arcuate nucleus (ARC), the periventricular nucleus (PeV), ventrolateral region of the ventromedial nucleus, the paraventricular nucleus and the supraoptic nucleus. To further understand the significance of the GHR gene expression in the hypothalamus, the effect of in vivo manipulation of GH on the somatostatin (SS) gene expression in the ARC and PeV, and the GRF gene expression in the ARC was observed among adult male rats using in situ hybridization histochemistry. Ten days after hypophysectomy, the SS mRNA level in the ARC as well as PeV was significantly lower than that in the respective nuclei of sham‐operated control rats, while the GRF mRNA level in the ARC was significantly higher than that in the ARC of control animals. Subcutaneous injection of recombinant human GH (0.33 mg) to hypophysectomized rats every 12 h for 5 days restored the SS mRNA level in the ARC and PeV, and reduced the GRF mRNA level in the ARC to that of control animals. The data suggest that GH directly acts on the hypothalamic PeV and ARC, and alters the gene expression of SS and GRF.</description><identifier>ISSN: 0953-8194</identifier><identifier>EISSN: 1365-2826</identifier><identifier>DOI: 10.1111/j.1365-2826.1993.tb00541.x</identifier><identifier>PMID: 8680443</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Arcuate Nucleus of Hypothalamus - metabolism ; Biological and medical sciences ; Blotting, Northern ; Cell receptors ; Cell structures and functions ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - physiology ; growth hormone receptor ; growth hormone releasing factor ; Growth Hormone-Releasing Hormone - pharmacology ; Histocytochemistry ; Hormone Antagonists - pharmacology ; Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors ; Hypophysectomy ; Hypothalamus - metabolism ; In Situ Hybridization ; Male ; messenger ribonucleic acid ; Molecular and cellular biology ; Paraventricular Hypothalamic Nucleus - metabolism ; Rats ; Rats, Wistar ; Receptors, Somatotropin - biosynthesis ; Receptors, Somatotropin - genetics ; RNA, Messenger - biosynthesis ; somatostatin ; Somatostatin - biosynthesis ; Somatostatin - genetics ; Somatostatin - pharmacology</subject><ispartof>Journal of neuroendocrinology, 1993-12, Vol.5 (6), p.691-696</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5021-3df78b48c1f249476644322250c62fda2dc4c360d3e2bbfed95e4d758b98e9373</citedby><cites>FETCH-LOGICAL-c5021-3df78b48c1f249476644322250c62fda2dc4c360d3e2bbfed95e4d758b98e9373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2826.1993.tb00541.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2826.1993.tb00541.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3839257$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8680443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Minami, Shiro</creatorcontrib><creatorcontrib>Kamegai, Jun</creatorcontrib><creatorcontrib>Hasegawa, Osamu</creatorcontrib><creatorcontrib>Sugihara, Hitoshi</creatorcontrib><creatorcontrib>Okada, Kenmei</creatorcontrib><creatorcontrib>Wakabayashi, Ichiji</creatorcontrib><title>Expression of Growth Hormone Receptor Gene in Rat Hypothalamus</title><title>Journal of neuroendocrinology</title><addtitle>J Neuroendocrinol</addtitle><description>Growth hormone receptor (GHR) mRNA‐expressing cells in the hypothalamus were observed using hybridization histochemistry in adult male rats. Digoxigenin‐labeled cRNA corresponding to the extracellular part of rat GHR was used as a probe. Northern blotting analysis of hypothalamic total RNA from adult male rats revealed that the 4.5 kilobase (kb) transcript of the GHR gene corresponding to the GHR messenger RNA (mRNA) predominated over the 1.2 kb transcript corresponding to GH‐binding protein mRNA. GHR mRNA‐containing cells were observed in the arcuate nucleus (ARC), the periventricular nucleus (PeV), ventrolateral region of the ventromedial nucleus, the paraventricular nucleus and the supraoptic nucleus. To further understand the significance of the GHR gene expression in the hypothalamus, the effect of in vivo manipulation of GH on the somatostatin (SS) gene expression in the ARC and PeV, and the GRF gene expression in the ARC was observed among adult male rats using in situ hybridization histochemistry. Ten days after hypophysectomy, the SS mRNA level in the ARC as well as PeV was significantly lower than that in the respective nuclei of sham‐operated control rats, while the GRF mRNA level in the ARC was significantly higher than that in the ARC of control animals. Subcutaneous injection of recombinant human GH (0.33 mg) to hypophysectomized rats every 12 h for 5 days restored the SS mRNA level in the ARC and PeV, and reduced the GRF mRNA level in the ARC to that of control animals. The data suggest that GH directly acts on the hypothalamic PeV and ARC, and alters the gene expression of SS and GRF.</description><subject>Animals</subject><subject>Arcuate Nucleus of Hypothalamus - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - physiology</subject><subject>growth hormone receptor</subject><subject>growth hormone releasing factor</subject><subject>Growth Hormone-Releasing Hormone - pharmacology</subject><subject>Histocytochemistry</subject><subject>Hormone Antagonists - pharmacology</subject><subject>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</subject><subject>Hypophysectomy</subject><subject>Hypothalamus - metabolism</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>messenger ribonucleic acid</subject><subject>Molecular and cellular biology</subject><subject>Paraventricular Hypothalamic Nucleus - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Somatotropin - biosynthesis</subject><subject>Receptors, Somatotropin - genetics</subject><subject>RNA, Messenger - biosynthesis</subject><subject>somatostatin</subject><subject>Somatostatin - biosynthesis</subject><subject>Somatostatin - genetics</subject><subject>Somatostatin - pharmacology</subject><issn>0953-8194</issn><issn>1365-2826</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkFuL1DAUx4Mo67j6EYQi4ltr7hcfFmSZnVHWVRdF8CWk6SnbsW1q0mFnvr0tU-bdvBzC_3IOP4TeEFyQ6b3fFYRJkVNNZUGMYcVYYiw4KQ5P0OosPUUrbATLNTH8OXqR0g5jogTDF-hCS405Zyt0tT4MEVJqQp-FOtvE8Dg-ZNsQu9BDdg8ehjHEbAPTr-mzezdm2-MQxgfXum6fXqJntWsTvFrmJfp5s_5xvc1vv24-XX-8zb3AlOSsqpUuufakptxwJeW0nFIqsJe0rhytPPdM4ooBLcsaKiOAV0ro0mgwTLFL9O7UO8Twdw9ptF2TPLSt6yHsk1VySjPFJ-OHk9HHkFKE2g6x6Vw8WoLtDM_u7EzIzoTsDM8u8OxhCr9etuzLDqpzdKE16W8X3SXv2jq63jfpbGOaGSrmY69OtsemheN_HGA_362lIVNBfipo0giHc4GLf6xUTAn7625jb77x39sv37nF7B9iO5nM</recordid><startdate>199312</startdate><enddate>199312</enddate><creator>Minami, Shiro</creator><creator>Kamegai, Jun</creator><creator>Hasegawa, Osamu</creator><creator>Sugihara, Hitoshi</creator><creator>Okada, Kenmei</creator><creator>Wakabayashi, Ichiji</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199312</creationdate><title>Expression of Growth Hormone Receptor Gene in Rat Hypothalamus</title><author>Minami, Shiro ; Kamegai, Jun ; Hasegawa, Osamu ; Sugihara, Hitoshi ; Okada, Kenmei ; Wakabayashi, Ichiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5021-3df78b48c1f249476644322250c62fda2dc4c360d3e2bbfed95e4d758b98e9373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Arcuate Nucleus of Hypothalamus - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - physiology</topic><topic>growth hormone receptor</topic><topic>growth hormone releasing factor</topic><topic>Growth Hormone-Releasing Hormone - pharmacology</topic><topic>Histocytochemistry</topic><topic>Hormone Antagonists - pharmacology</topic><topic>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</topic><topic>Hypophysectomy</topic><topic>Hypothalamus - metabolism</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>messenger ribonucleic acid</topic><topic>Molecular and cellular biology</topic><topic>Paraventricular Hypothalamic Nucleus - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Somatotropin - biosynthesis</topic><topic>Receptors, Somatotropin - genetics</topic><topic>RNA, Messenger - biosynthesis</topic><topic>somatostatin</topic><topic>Somatostatin - biosynthesis</topic><topic>Somatostatin - genetics</topic><topic>Somatostatin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Minami, Shiro</creatorcontrib><creatorcontrib>Kamegai, Jun</creatorcontrib><creatorcontrib>Hasegawa, Osamu</creatorcontrib><creatorcontrib>Sugihara, Hitoshi</creatorcontrib><creatorcontrib>Okada, Kenmei</creatorcontrib><creatorcontrib>Wakabayashi, Ichiji</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroendocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Minami, Shiro</au><au>Kamegai, Jun</au><au>Hasegawa, Osamu</au><au>Sugihara, Hitoshi</au><au>Okada, Kenmei</au><au>Wakabayashi, Ichiji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Growth Hormone Receptor Gene in Rat Hypothalamus</atitle><jtitle>Journal of neuroendocrinology</jtitle><addtitle>J Neuroendocrinol</addtitle><date>1993-12</date><risdate>1993</risdate><volume>5</volume><issue>6</issue><spage>691</spage><epage>696</epage><pages>691-696</pages><issn>0953-8194</issn><eissn>1365-2826</eissn><abstract>Growth hormone receptor (GHR) mRNA‐expressing cells in the hypothalamus were observed using hybridization histochemistry in adult male rats. Digoxigenin‐labeled cRNA corresponding to the extracellular part of rat GHR was used as a probe. Northern blotting analysis of hypothalamic total RNA from adult male rats revealed that the 4.5 kilobase (kb) transcript of the GHR gene corresponding to the GHR messenger RNA (mRNA) predominated over the 1.2 kb transcript corresponding to GH‐binding protein mRNA. GHR mRNA‐containing cells were observed in the arcuate nucleus (ARC), the periventricular nucleus (PeV), ventrolateral region of the ventromedial nucleus, the paraventricular nucleus and the supraoptic nucleus. To further understand the significance of the GHR gene expression in the hypothalamus, the effect of in vivo manipulation of GH on the somatostatin (SS) gene expression in the ARC and PeV, and the GRF gene expression in the ARC was observed among adult male rats using in situ hybridization histochemistry. Ten days after hypophysectomy, the SS mRNA level in the ARC as well as PeV was significantly lower than that in the respective nuclei of sham‐operated control rats, while the GRF mRNA level in the ARC was significantly higher than that in the ARC of control animals. Subcutaneous injection of recombinant human GH (0.33 mg) to hypophysectomized rats every 12 h for 5 days restored the SS mRNA level in the ARC and PeV, and reduced the GRF mRNA level in the ARC to that of control animals. The data suggest that GH directly acts on the hypothalamic PeV and ARC, and alters the gene expression of SS and GRF.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8680443</pmid><doi>10.1111/j.1365-2826.1993.tb00541.x</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Arcuate Nucleus of Hypothalamus - metabolism Biological and medical sciences Blotting, Northern Cell receptors Cell structures and functions Fundamental and applied biological sciences. Psychology Gene Expression Regulation - physiology growth hormone receptor growth hormone releasing factor Growth Hormone-Releasing Hormone - pharmacology Histocytochemistry Hormone Antagonists - pharmacology Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors Hypophysectomy Hypothalamus - metabolism In Situ Hybridization Male messenger ribonucleic acid Molecular and cellular biology Paraventricular Hypothalamic Nucleus - metabolism Rats Rats, Wistar Receptors, Somatotropin - biosynthesis Receptors, Somatotropin - genetics RNA, Messenger - biosynthesis somatostatin Somatostatin - biosynthesis Somatostatin - genetics Somatostatin - pharmacology |
| title | Expression of Growth Hormone Receptor Gene in Rat Hypothalamus |
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