Degradation of D2 protein due to UV-B irradiation of the reaction centre of photosystem II
Exposure of isolated reaction centres of photosystem II to UV-B radiation generates specific breakdown products of the D2 protein. When the quinone, 2,5-dibromo-3-methyl-6-isopropyl- p-benzoquinone is present a 22 kDa fragment containing the N-terminus of the mature protein is generated. Concomitant...
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| Veröffentlicht in: | FEBS letters 1994-02, Vol.339 (3), p.217-221 |
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| creator | Friso, G. Barbato, R. Giacometti, G.M. Barber, J. |
| description | Exposure of isolated reaction centres of photosystem II to UV-B radiation generates specific breakdown products of the D2 protein. When the quinone, 2,5-dibromo-3-methyl-6-isopropyl-
p-benzoquinone is present a 22 kDa fragment containing the N-terminus of the mature protein is generated. Concomitant with the appearance of the N-terminal fragment, two fragments containing the C-terminus of the D2 protein having apparent molecular masses around 10–12 kDa are observed. It is concluded that the primary cleavage occurs in the hydrophilic loop linking putative transmembrane segments IV and V. No such cleavage was observed when silicomolybdate was used as an electron acceptor, suggesting that this UV-B damage is dependent on binding of the added quinone to the Q
A site. |
| doi_str_mv | 10.1016/0014-5793(94)80419-2 |
| format | Article |
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p-benzoquinone is present a 22 kDa fragment containing the N-terminus of the mature protein is generated. Concomitant with the appearance of the N-terminal fragment, two fragments containing the C-terminus of the D2 protein having apparent molecular masses around 10–12 kDa are observed. It is concluded that the primary cleavage occurs in the hydrophilic loop linking putative transmembrane segments IV and V. No such cleavage was observed when silicomolybdate was used as an electron acceptor, suggesting that this UV-B damage is dependent on binding of the added quinone to the Q
A site.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(94)80419-2</identifier><identifier>PMID: 8112459</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Benzoquinones - metabolism ; Binding Sites ; Biological and medical sciences ; Chl, chlorophyll ; D2 protein ; DBMIB, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone ; Dibromothymoquinone - pharmacology ; Electrophoresis, Gel, Two-Dimensional ; Fundamental and applied biological sciences. Psychology ; Immunoblotting ; Molecular biophysics ; Molecular Weight ; P680, primary donor in photosystem II, PSII, photosystem II ; PAGE, polyacrylamide gel electrophoresis ; Peptide Fragments - metabolism ; pheo, pheophytin ; Photochemistry. Photosynthesis. Bioluminescence ; Photoinactivation ; Photosynthetic Reaction Center Complex Proteins - metabolism ; Photosynthetic Reaction Center Complex Proteins - radiation effects ; Photosystem II ; Photosystem II Protein Complex ; PVDF, polyvinylidenedifluoride ; Radiation-biomolecule interaction ; SDS, sodium dodecyi sulphate ; Serine Endopeptidases - metabolism ; Ultraviolet Rays ; UVB light</subject><ispartof>FEBS letters, 1994-02, Vol.339 (3), p.217-221</ispartof><rights>1994</rights><rights>FEBS Letters 339 (1994) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4952-a4db038848e6388bd09f35c1ff15b2aae4eb147fd7d22823d4cd795de7f802883</citedby><cites>FETCH-LOGICAL-c4952-a4db038848e6388bd09f35c1ff15b2aae4eb147fd7d22823d4cd795de7f802883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579394804192$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3992479$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8112459$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Friso, G.</creatorcontrib><creatorcontrib>Barbato, R.</creatorcontrib><creatorcontrib>Giacometti, G.M.</creatorcontrib><creatorcontrib>Barber, J.</creatorcontrib><title>Degradation of D2 protein due to UV-B irradiation of the reaction centre of photosystem II</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Exposure of isolated reaction centres of photosystem II to UV-B radiation generates specific breakdown products of the D2 protein. When the quinone, 2,5-dibromo-3-methyl-6-isopropyl-
p-benzoquinone is present a 22 kDa fragment containing the N-terminus of the mature protein is generated. Concomitant with the appearance of the N-terminal fragment, two fragments containing the C-terminus of the D2 protein having apparent molecular masses around 10–12 kDa are observed. It is concluded that the primary cleavage occurs in the hydrophilic loop linking putative transmembrane segments IV and V. No such cleavage was observed when silicomolybdate was used as an electron acceptor, suggesting that this UV-B damage is dependent on binding of the added quinone to the Q
A site.</description><subject>Benzoquinones - metabolism</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Chl, chlorophyll</subject><subject>D2 protein</subject><subject>DBMIB, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone</subject><subject>Dibromothymoquinone - pharmacology</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoblotting</subject><subject>Molecular biophysics</subject><subject>Molecular Weight</subject><subject>P680, primary donor in photosystem II, PSII, photosystem II</subject><subject>PAGE, polyacrylamide gel electrophoresis</subject><subject>Peptide Fragments - metabolism</subject><subject>pheo, pheophytin</subject><subject>Photochemistry. Photosynthesis. Bioluminescence</subject><subject>Photoinactivation</subject><subject>Photosynthetic Reaction Center Complex Proteins - metabolism</subject><subject>Photosynthetic Reaction Center Complex Proteins - radiation effects</subject><subject>Photosystem II</subject><subject>Photosystem II Protein Complex</subject><subject>PVDF, polyvinylidenedifluoride</subject><subject>Radiation-biomolecule interaction</subject><subject>SDS, sodium dodecyi sulphate</subject><subject>Serine Endopeptidases - metabolism</subject><subject>Ultraviolet Rays</subject><subject>UVB light</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1vEzEQhi0EKqHwD0DyAaFyWPDXru0LEv0IRKrEhXLgYnntMTXarFPbocq_ZzeJckScRvO-z4zHL0KvKflACe0-EkJF00rNL7R4r4igumFP0IIqyRsuOvUULU7Ic_SilN9k6hXVZ-hMUcpEqxfo5zX8ytbbGtOIU8DXDG9yqhBH7LeAa8J3P5pLHPMExRNV7wFnsG7fOxhrhlne3Keayq5UWOPV6iV6FuxQ4NWxnqO75c33q6_N7bcvq6vPt40TumWNFb4nXCmhoJtK74kOvHU0BNr2zFoQ0FMhg5eeMcW4F85L3XqQQRGmFD9H7w57p8MftlCqWcfiYBjsCGlbjOx4q2TXTqA4gC6nUjIEs8lxbfPOUGLmSM2cl5nzMlqYfaSGTWNvjvu3_Rr8aeiY4eS_Pfq2ODuEbEcXywnjWjMhZ2x5wB7jALv_etosby7ZbMy6Fnt1vufTYRFMof6JkE1xEUYHPmZw1fgU__2hv5MnpHg</recordid><startdate>19940221</startdate><enddate>19940221</enddate><creator>Friso, G.</creator><creator>Barbato, R.</creator><creator>Giacometti, G.M.</creator><creator>Barber, J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940221</creationdate><title>Degradation of D2 protein due to UV-B irradiation of the reaction centre of photosystem II</title><author>Friso, G. ; Barbato, R. ; Giacometti, G.M. ; Barber, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4952-a4db038848e6388bd09f35c1ff15b2aae4eb147fd7d22823d4cd795de7f802883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Benzoquinones - metabolism</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Chl, chlorophyll</topic><topic>D2 protein</topic><topic>DBMIB, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone</topic><topic>Dibromothymoquinone - pharmacology</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoblotting</topic><topic>Molecular biophysics</topic><topic>Molecular Weight</topic><topic>P680, primary donor in photosystem II, PSII, photosystem II</topic><topic>PAGE, polyacrylamide gel electrophoresis</topic><topic>Peptide Fragments - metabolism</topic><topic>pheo, pheophytin</topic><topic>Photochemistry. Photosynthesis. Bioluminescence</topic><topic>Photoinactivation</topic><topic>Photosynthetic Reaction Center Complex Proteins - metabolism</topic><topic>Photosynthetic Reaction Center Complex Proteins - radiation effects</topic><topic>Photosystem II</topic><topic>Photosystem II Protein Complex</topic><topic>PVDF, polyvinylidenedifluoride</topic><topic>Radiation-biomolecule interaction</topic><topic>SDS, sodium dodecyi sulphate</topic><topic>Serine Endopeptidases - metabolism</topic><topic>Ultraviolet Rays</topic><topic>UVB light</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Friso, G.</creatorcontrib><creatorcontrib>Barbato, R.</creatorcontrib><creatorcontrib>Giacometti, G.M.</creatorcontrib><creatorcontrib>Barber, J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Friso, G.</au><au>Barbato, R.</au><au>Giacometti, G.M.</au><au>Barber, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Degradation of D2 protein due to UV-B irradiation of the reaction centre of photosystem II</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1994-02-21</date><risdate>1994</risdate><volume>339</volume><issue>3</issue><spage>217</spage><epage>221</epage><pages>217-221</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Exposure of isolated reaction centres of photosystem II to UV-B radiation generates specific breakdown products of the D2 protein. When the quinone, 2,5-dibromo-3-methyl-6-isopropyl-
p-benzoquinone is present a 22 kDa fragment containing the N-terminus of the mature protein is generated. Concomitant with the appearance of the N-terminal fragment, two fragments containing the C-terminus of the D2 protein having apparent molecular masses around 10–12 kDa are observed. It is concluded that the primary cleavage occurs in the hydrophilic loop linking putative transmembrane segments IV and V. No such cleavage was observed when silicomolybdate was used as an electron acceptor, suggesting that this UV-B damage is dependent on binding of the added quinone to the Q
A site.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8112459</pmid><doi>10.1016/0014-5793(94)80419-2</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1994-02, Vol.339 (3), p.217-221 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Benzoquinones - metabolism Binding Sites Biological and medical sciences Chl, chlorophyll D2 protein DBMIB, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone Dibromothymoquinone - pharmacology Electrophoresis, Gel, Two-Dimensional Fundamental and applied biological sciences. Psychology Immunoblotting Molecular biophysics Molecular Weight P680, primary donor in photosystem II, PSII, photosystem II PAGE, polyacrylamide gel electrophoresis Peptide Fragments - metabolism pheo, pheophytin Photochemistry. Photosynthesis. Bioluminescence Photoinactivation Photosynthetic Reaction Center Complex Proteins - metabolism Photosynthetic Reaction Center Complex Proteins - radiation effects Photosystem II Photosystem II Protein Complex PVDF, polyvinylidenedifluoride Radiation-biomolecule interaction SDS, sodium dodecyi sulphate Serine Endopeptidases - metabolism Ultraviolet Rays UVB light |
| title | Degradation of D2 protein due to UV-B irradiation of the reaction centre of photosystem II |
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