Detection of Mycobacterium paratuberculosis by Polymerase Chain Reaction in Children with Crohn's Disease
Mycobacterium paratuberculosis, the causative agent of Johne's disease (a chronic enteritis in ruminants), has been suspected to be involved in Crohn's disease. In this study, polymerase chain reaction was used to detect the presence of IS900DNA sequences specific to M. paratuberculosis ge...
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| Veröffentlicht in: | The Journal of infectious diseases 1994-02, Vol.169 (2), p.449-451 |
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| container_title | The Journal of infectious diseases |
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| creator | Dell'Isola, Bruno Poyart, Claire Goulet, Olivier Mougenot, Jean Francois Journo, Evelyne Sadoun Brousse, Nicole Schmitz, Jacques Ricour, Claude Berche, Patrick |
| description | Mycobacterium paratuberculosis, the causative agent of Johne's disease (a chronic enteritis in ruminants), has been suspected to be involved in Crohn's disease. In this study, polymerase chain reaction was used to detect the presence of IS900DNA sequences specific to M. paratuberculosis genomes in biopsies and surgical resections from 53 children with various gastrointestinal diseases and disorders. ISO900sequences were found in 13 of 18 samples from patients with Crohn's disease (72%; P < .01 vs. samples from patients without Crohn's disease), in 1 of 5 with ulcerative colitis, in 2 of 6 with severe unclassified colitis, and in 7 of 24 with other gastrointestinal illnesses. These results appear to support the hypothesis that M. paratuberculosis is involved in the pathogenesis of Crohn's disease. |
| doi_str_mv | 10.1093/infdis/169.2.449 |
| format | Article |
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In this study, polymerase chain reaction was used to detect the presence of IS900DNA sequences specific to M. paratuberculosis genomes in biopsies and surgical resections from 53 children with various gastrointestinal diseases and disorders. ISO900sequences were found in 13 of 18 samples from patients with Crohn's disease (72%; P < .01 vs. samples from patients without Crohn's disease), in 1 of 5 with ulcerative colitis, in 2 of 6 with severe unclassified colitis, and in 7 of 24 with other gastrointestinal illnesses. These results appear to support the hypothesis that M. paratuberculosis is involved in the pathogenesis of Crohn's disease.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/169.2.449</identifier><identifier>PMID: 8106782</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Biological and medical sciences ; Biopsies ; Child, Preschool ; Colitis ; Concise Communications ; Crohn disease ; Crohn Disease - complications ; Crohn Disease - microbiology ; DNA ; DNA Primers ; DNA, Bacterial - analysis ; Gastroenterology. Liver. Pancreas. Abdomen ; Gastrointestinal diseases ; Humans ; Infectious diseases ; Medical sciences ; Mycobacterium avium subsp. paratuberculosis - genetics ; Mycobacterium paratuberculosis ; Other diseases. Semiology ; Paratuberculosis ; Paratuberculosis - diagnosis ; Polymerase Chain Reaction ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Tissue samples</subject><ispartof>The Journal of infectious diseases, 1994-02, Vol.169 (2), p.449-451</ispartof><rights>Copyright 1994 The University of Chicago</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-28d8cf097ee438270fe414d5c6e4f2cd464166420f254582fd8e4fc3028684ac3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30113933$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30113933$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4073545$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8106782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dell'Isola, Bruno</creatorcontrib><creatorcontrib>Poyart, Claire</creatorcontrib><creatorcontrib>Goulet, Olivier</creatorcontrib><creatorcontrib>Mougenot, Jean Francois</creatorcontrib><creatorcontrib>Journo, Evelyne Sadoun</creatorcontrib><creatorcontrib>Brousse, Nicole</creatorcontrib><creatorcontrib>Schmitz, Jacques</creatorcontrib><creatorcontrib>Ricour, Claude</creatorcontrib><creatorcontrib>Berche, Patrick</creatorcontrib><title>Detection of Mycobacterium paratuberculosis by Polymerase Chain Reaction in Children with Crohn's Disease</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Mycobacterium paratuberculosis, the causative agent of Johne's disease (a chronic enteritis in ruminants), has been suspected to be involved in Crohn's disease. In this study, polymerase chain reaction was used to detect the presence of IS900DNA sequences specific to M. paratuberculosis genomes in biopsies and surgical resections from 53 children with various gastrointestinal diseases and disorders. ISO900sequences were found in 13 of 18 samples from patients with Crohn's disease (72%; P < .01 vs. samples from patients without Crohn's disease), in 1 of 5 with ulcerative colitis, in 2 of 6 with severe unclassified colitis, and in 7 of 24 with other gastrointestinal illnesses. These results appear to support the hypothesis that M. paratuberculosis is involved in the pathogenesis of Crohn's disease.</description><subject>Biological and medical sciences</subject><subject>Biopsies</subject><subject>Child, Preschool</subject><subject>Colitis</subject><subject>Concise Communications</subject><subject>Crohn disease</subject><subject>Crohn Disease - complications</subject><subject>Crohn Disease - microbiology</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - analysis</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gastrointestinal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Mycobacterium avium subsp. paratuberculosis - genetics</subject><subject>Mycobacterium paratuberculosis</subject><subject>Other diseases. Semiology</subject><subject>Paratuberculosis</subject><subject>Paratuberculosis - diagnosis</subject><subject>Polymerase Chain Reaction</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. 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Liver. Pancreas. Abdomen</topic><topic>Gastrointestinal diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Mycobacterium avium subsp. paratuberculosis - genetics</topic><topic>Mycobacterium paratuberculosis</topic><topic>Other diseases. Semiology</topic><topic>Paratuberculosis</topic><topic>Paratuberculosis - diagnosis</topic><topic>Polymerase Chain Reaction</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. 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In this study, polymerase chain reaction was used to detect the presence of IS900DNA sequences specific to M. paratuberculosis genomes in biopsies and surgical resections from 53 children with various gastrointestinal diseases and disorders. ISO900sequences were found in 13 of 18 samples from patients with Crohn's disease (72%; P < .01 vs. samples from patients without Crohn's disease), in 1 of 5 with ulcerative colitis, in 2 of 6 with severe unclassified colitis, and in 7 of 24 with other gastrointestinal illnesses. These results appear to support the hypothesis that M. paratuberculosis is involved in the pathogenesis of Crohn's disease.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>8106782</pmid><doi>10.1093/infdis/169.2.449</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0022-1899 |
| ispartof | The Journal of infectious diseases, 1994-02, Vol.169 (2), p.449-451 |
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| subjects | Biological and medical sciences Biopsies Child, Preschool Colitis Concise Communications Crohn disease Crohn Disease - complications Crohn Disease - microbiology DNA DNA Primers DNA, Bacterial - analysis Gastroenterology. Liver. Pancreas. Abdomen Gastrointestinal diseases Humans Infectious diseases Medical sciences Mycobacterium avium subsp. paratuberculosis - genetics Mycobacterium paratuberculosis Other diseases. Semiology Paratuberculosis Paratuberculosis - diagnosis Polymerase Chain Reaction Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tissue samples |
| title | Detection of Mycobacterium paratuberculosis by Polymerase Chain Reaction in Children with Crohn's Disease |
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