Reversible conformational transition gives rise to ‘zig‐zag’ temperature dependence of the rate constant of irreversible thermoinactivation of enzymes

We have obtained unusual ‘zig‐zag’ temperature dependencies of the rate constant of irreversible thermoinactivation (kin) of enzymes (α‐chymotrypsin, covalently modified α‐chymotrypsin, and ribonuclease) in a plot of log kin versus reciprocal temperature (Arrhenius plot). These dependencies are char...

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Veröffentlicht in:	European journal of biochemistry 1994-01, Vol.219 (1‐2), p.219-230
Hauptverfasser:	LEVITSKY, Vladislav Yu, MELIK‐NUBAROV, Nikolay S., SIKSNIS, Virginius A., GRINBERG, Valeriy Ya, BUROVA, Tatyana V., LEVASHOV, Andrey V., MOZHAEV, Vadim V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Calorimetry, Differential Scanning Chromatography, Affinity Chymotrypsin - chemistry Chymotrypsin - isolation & purification Chymotrypsin - metabolism Enzyme Stability Enzymes - chemistry Hot Temperature Kinetics Protein Conformation Protein Denaturation Ribonucleases - antagonists & inhibitors Ribonucleases - chemistry Ribonucleases - metabolism Thiocyanates - pharmacology
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