p135tyk2, an interferon-alpha-activated tyrosine kinase, is physically associated with an interferon-alpha receptor
Recent genetic studies have linked the tyk2 gene, which encodes a novel type of non-receptor tyrosine kinase, to the interferon-alpha intracellular signaling pathway. In this report, biochemical evidence is presented which supports this proposed function for the tyk2 tyrosine kinase and further defi...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1994-02, Vol.269 (5), p.3518-3522 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3522 |
|---|---|
| container_issue | 5 |
| container_start_page | 3518 |
| container_title | The Journal of biological chemistry |
| container_volume | 269 |
| creator | COLAMONICI, O. R UYTTENDAELE, H DOMANSKI, P HAI YAN KROLEWSKI, J. J |
| description | Recent genetic studies have linked the tyk2 gene, which encodes a novel type of non-receptor tyrosine kinase, to the interferon-alpha
intracellular signaling pathway. In this report, biochemical evidence is presented which supports this proposed function for
the tyk2 tyrosine kinase and further defines its role in the interferon-alpha signaling cascade. Specifically, the tyk2 gene
is shown to encode a 135-kDa protein which is rapidly phosphorylated on tyrosine in response to interferon-alpha treatment.
Indirect evidence suggests that the tyrosine phosphorylation of p135tyk2 is the result of autokinase activity, implying that
the Tyk2 tyrosine kinase is activated by interferon-alpha treatment. Two complementary methods demonstrate a physical association
between p135tyk2 and the alpha-subunit of the interferon-alpha receptor. First, immunoblots show that monoclonal antibodies
against the alpha-subunit of the interferon-alpha receptor can co-immunoprecipitate p135tyk2. Second, interferon-alpha receptor
proteins which have been labeled by affinity cross-linking with 125I-interferon-alpha 2 can be co-immunoprecipitated using
anti-tyk2 antisera. Taken together, these data suggest that an interferon-alpha receptor-p135tyk2 complex functions, in a
manner analogous to the CD4-lck tyrosine kinase complex, to initiate the interferon-alpha signaling cascade. |
| doi_str_mv | 10.1016/S0021-9258(17)41893-X |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76353923</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76353923</sourcerecordid><originalsourceid>FETCH-LOGICAL-c254x-5af0b8998ca5785d5e11266c8b2e301451ce1f54cb54aa9d0379d111a016a3513</originalsourceid><addsrcrecordid>eNptkEtP3DAQgC1URLfQn4AUVVXVSoR64jixjwi1pRISB0DamzVxJsQlm6R2tjT_Hu9De2Iuc5hvXh9j58AvgUPx_Z7zDFKdSfUVym85KC3S5RFbAFciFRKW79jigLxnH0L4w2PkGk7YiQJeCC0WLIwg5DQ_ZxcJ9onrJ_IN-aFPsRtbTNFO7h9OVCfT7IfgekqeXY-BLhIXkrGdg7PYdXOCIQzWbckXN7VvDUs8WRqnwZ-x4wa7QB_3-ZQ9_vzxcH2T3t79-n19dZvaTOb_U4kNr5TWyqIslawlAWRFYVWVkeCQS7AEjcxtJXNEXXNR6hoAMNrBKECcsi-7uaMf_q4pTGblgqWuw56GdTBlIaTQmYig3IE2_hg8NWb0boV-NsDNRrbZyjYbkwZKs5VtlrHvfL9gXa2oPnTt7cb6530dQ9TUeOytCwcs5xxEsbnz0w5r3VP74jyZyg22pZXJCm2kib8o8Qp_epOR</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76353923</pqid></control><display><type>article</type><title>p135tyk2, an interferon-alpha-activated tyrosine kinase, is physically associated with an interferon-alpha receptor</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>COLAMONICI, O. R ; UYTTENDAELE, H ; DOMANSKI, P ; HAI YAN ; KROLEWSKI, J. J</creator><creatorcontrib>COLAMONICI, O. R ; UYTTENDAELE, H ; DOMANSKI, P ; HAI YAN ; KROLEWSKI, J. J</creatorcontrib><description>Recent genetic studies have linked the tyk2 gene, which encodes a novel type of non-receptor tyrosine kinase, to the interferon-alpha
intracellular signaling pathway. In this report, biochemical evidence is presented which supports this proposed function for
the tyk2 tyrosine kinase and further defines its role in the interferon-alpha signaling cascade. Specifically, the tyk2 gene
is shown to encode a 135-kDa protein which is rapidly phosphorylated on tyrosine in response to interferon-alpha treatment.
Indirect evidence suggests that the tyrosine phosphorylation of p135tyk2 is the result of autokinase activity, implying that
the Tyk2 tyrosine kinase is activated by interferon-alpha treatment. Two complementary methods demonstrate a physical association
between p135tyk2 and the alpha-subunit of the interferon-alpha receptor. First, immunoblots show that monoclonal antibodies
against the alpha-subunit of the interferon-alpha receptor can co-immunoprecipitate p135tyk2. Second, interferon-alpha receptor
proteins which have been labeled by affinity cross-linking with 125I-interferon-alpha 2 can be co-immunoprecipitated using
anti-tyk2 antisera. Taken together, these data suggest that an interferon-alpha receptor-p135tyk2 complex functions, in a
manner analogous to the CD4-lck tyrosine kinase complex, to initiate the interferon-alpha signaling cascade.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(17)41893-X</identifier><identifier>PMID: 8106393</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Animals ; Antibodies, Monoclonal ; Base Sequence ; Biological and medical sciences ; Cell Line ; Cell receptors ; Cell structures and functions ; Cloning, Molecular ; DNA Primers ; DNA, Complementary - metabolism ; Enzyme Activation ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; HeLa Cells ; Humans ; Immunoblotting ; Interferon-alpha - metabolism ; Interferon-alpha - pharmacology ; Miscellaneous ; Molecular and cellular biology ; Molecular Sequence Data ; Moths ; Protein Biosynthesis ; Protein-Tyrosine Kinases - metabolism ; Proteins - isolation & purification ; Proteins - metabolism ; Receptor, Interferon alpha-beta ; Receptors, Interferon - metabolism ; Recombinant Fusion Proteins - biosynthesis ; Recombinant Fusion Proteins - metabolism ; Signal Transduction ; Transfection ; TYK2 Kinase</subject><ispartof>The Journal of biological chemistry, 1994-02, Vol.269 (5), p.3518-3522</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c254x-5af0b8998ca5785d5e11266c8b2e301451ce1f54cb54aa9d0379d111a016a3513</citedby><cites>FETCH-LOGICAL-c254x-5af0b8998ca5785d5e11266c8b2e301451ce1f54cb54aa9d0379d111a016a3513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27926,27927</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4001361$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8106393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>COLAMONICI, O. R</creatorcontrib><creatorcontrib>UYTTENDAELE, H</creatorcontrib><creatorcontrib>DOMANSKI, P</creatorcontrib><creatorcontrib>HAI YAN</creatorcontrib><creatorcontrib>KROLEWSKI, J. J</creatorcontrib><title>p135tyk2, an interferon-alpha-activated tyrosine kinase, is physically associated with an interferon-alpha receptor</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Recent genetic studies have linked the tyk2 gene, which encodes a novel type of non-receptor tyrosine kinase, to the interferon-alpha
intracellular signaling pathway. In this report, biochemical evidence is presented which supports this proposed function for
the tyk2 tyrosine kinase and further defines its role in the interferon-alpha signaling cascade. Specifically, the tyk2 gene
is shown to encode a 135-kDa protein which is rapidly phosphorylated on tyrosine in response to interferon-alpha treatment.
Indirect evidence suggests that the tyrosine phosphorylation of p135tyk2 is the result of autokinase activity, implying that
the Tyk2 tyrosine kinase is activated by interferon-alpha treatment. Two complementary methods demonstrate a physical association
between p135tyk2 and the alpha-subunit of the interferon-alpha receptor. First, immunoblots show that monoclonal antibodies
against the alpha-subunit of the interferon-alpha receptor can co-immunoprecipitate p135tyk2. Second, interferon-alpha receptor
proteins which have been labeled by affinity cross-linking with 125I-interferon-alpha 2 can be co-immunoprecipitated using
anti-tyk2 antisera. Taken together, these data suggest that an interferon-alpha receptor-p135tyk2 complex functions, in a
manner analogous to the CD4-lck tyrosine kinase complex, to initiate the interferon-alpha signaling cascade.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Cloning, Molecular</subject><subject>DNA Primers</subject><subject>DNA, Complementary - metabolism</subject><subject>Enzyme Activation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Interferon-alpha - metabolism</subject><subject>Interferon-alpha - pharmacology</subject><subject>Miscellaneous</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Moths</subject><subject>Protein Biosynthesis</subject><subject>Protein-Tyrosine Kinases - metabolism</subject><subject>Proteins - isolation & purification</subject><subject>Proteins - metabolism</subject><subject>Receptor, Interferon alpha-beta</subject><subject>Receptors, Interferon - metabolism</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Signal Transduction</subject><subject>Transfection</subject><subject>TYK2 Kinase</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEtP3DAQgC1URLfQn4AUVVXVSoR64jixjwi1pRISB0DamzVxJsQlm6R2tjT_Hu9De2Iuc5hvXh9j58AvgUPx_Z7zDFKdSfUVym85KC3S5RFbAFciFRKW79jigLxnH0L4w2PkGk7YiQJeCC0WLIwg5DQ_ZxcJ9onrJ_IN-aFPsRtbTNFO7h9OVCfT7IfgekqeXY-BLhIXkrGdg7PYdXOCIQzWbckXN7VvDUs8WRqnwZ-x4wa7QB_3-ZQ9_vzxcH2T3t79-n19dZvaTOb_U4kNr5TWyqIslawlAWRFYVWVkeCQS7AEjcxtJXNEXXNR6hoAMNrBKECcsi-7uaMf_q4pTGblgqWuw56GdTBlIaTQmYig3IE2_hg8NWb0boV-NsDNRrbZyjYbkwZKs5VtlrHvfL9gXa2oPnTt7cb6530dQ9TUeOytCwcs5xxEsbnz0w5r3VP74jyZyg22pZXJCm2kib8o8Qp_epOR</recordid><startdate>19940204</startdate><enddate>19940204</enddate><creator>COLAMONICI, O. R</creator><creator>UYTTENDAELE, H</creator><creator>DOMANSKI, P</creator><creator>HAI YAN</creator><creator>KROLEWSKI, J. J</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940204</creationdate><title>p135tyk2, an interferon-alpha-activated tyrosine kinase, is physically associated with an interferon-alpha receptor</title><author>COLAMONICI, O. R ; UYTTENDAELE, H ; DOMANSKI, P ; HAI YAN ; KROLEWSKI, J. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c254x-5af0b8998ca5785d5e11266c8b2e301451ce1f54cb54aa9d0379d111a016a3513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Cloning, Molecular</topic><topic>DNA Primers</topic><topic>DNA, Complementary - metabolism</topic><topic>Enzyme Activation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Interferon-alpha - metabolism</topic><topic>Interferon-alpha - pharmacology</topic><topic>Miscellaneous</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Moths</topic><topic>Protein Biosynthesis</topic><topic>Protein-Tyrosine Kinases - metabolism</topic><topic>Proteins - isolation & purification</topic><topic>Proteins - metabolism</topic><topic>Receptor, Interferon alpha-beta</topic><topic>Receptors, Interferon - metabolism</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Signal Transduction</topic><topic>Transfection</topic><topic>TYK2 Kinase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>COLAMONICI, O. R</creatorcontrib><creatorcontrib>UYTTENDAELE, H</creatorcontrib><creatorcontrib>DOMANSKI, P</creatorcontrib><creatorcontrib>HAI YAN</creatorcontrib><creatorcontrib>KROLEWSKI, J. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>COLAMONICI, O. R</au><au>UYTTENDAELE, H</au><au>DOMANSKI, P</au><au>HAI YAN</au><au>KROLEWSKI, J. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>p135tyk2, an interferon-alpha-activated tyrosine kinase, is physically associated with an interferon-alpha receptor</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-02-04</date><risdate>1994</risdate><volume>269</volume><issue>5</issue><spage>3518</spage><epage>3522</epage><pages>3518-3522</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Recent genetic studies have linked the tyk2 gene, which encodes a novel type of non-receptor tyrosine kinase, to the interferon-alpha
intracellular signaling pathway. In this report, biochemical evidence is presented which supports this proposed function for
the tyk2 tyrosine kinase and further defines its role in the interferon-alpha signaling cascade. Specifically, the tyk2 gene
is shown to encode a 135-kDa protein which is rapidly phosphorylated on tyrosine in response to interferon-alpha treatment.
Indirect evidence suggests that the tyrosine phosphorylation of p135tyk2 is the result of autokinase activity, implying that
the Tyk2 tyrosine kinase is activated by interferon-alpha treatment. Two complementary methods demonstrate a physical association
between p135tyk2 and the alpha-subunit of the interferon-alpha receptor. First, immunoblots show that monoclonal antibodies
against the alpha-subunit of the interferon-alpha receptor can co-immunoprecipitate p135tyk2. Second, interferon-alpha receptor
proteins which have been labeled by affinity cross-linking with 125I-interferon-alpha 2 can be co-immunoprecipitated using
anti-tyk2 antisera. Taken together, these data suggest that an interferon-alpha receptor-p135tyk2 complex functions, in a
manner analogous to the CD4-lck tyrosine kinase complex, to initiate the interferon-alpha signaling cascade.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8106393</pmid><doi>10.1016/S0021-9258(17)41893-X</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1994-02, Vol.269 (5), p.3518-3522 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76353923 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antibodies, Monoclonal Base Sequence Biological and medical sciences Cell Line Cell receptors Cell structures and functions Cloning, Molecular DNA Primers DNA, Complementary - metabolism Enzyme Activation Fundamental and applied biological sciences. Psychology Gene Expression HeLa Cells Humans Immunoblotting Interferon-alpha - metabolism Interferon-alpha - pharmacology Miscellaneous Molecular and cellular biology Molecular Sequence Data Moths Protein Biosynthesis Protein-Tyrosine Kinases - metabolism Proteins - isolation & purification Proteins - metabolism Receptor, Interferon alpha-beta Receptors, Interferon - metabolism Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - metabolism Signal Transduction Transfection TYK2 Kinase |
| title | p135tyk2, an interferon-alpha-activated tyrosine kinase, is physically associated with an interferon-alpha receptor |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T07%3A18%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=p135tyk2,%20an%20interferon-alpha-activated%20tyrosine%20kinase,%20is%20physically%20associated%20with%20an%20interferon-alpha%20receptor&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=COLAMONICI,%20O.%20R&rft.date=1994-02-04&rft.volume=269&rft.issue=5&rft.spage=3518&rft.epage=3522&rft.pages=3518-3522&rft.issn=0021-9258&rft.eissn=1083-351X&rft.coden=JBCHA3&rft_id=info:doi/10.1016/S0021-9258(17)41893-X&rft_dat=%3Cproquest_cross%3E76353923%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76353923&rft_id=info:pmid/8106393&rfr_iscdi=true |