The Labeling of Proliferating Cells by Ki67 and MIB-1 Antibodies Depends on the Binding of a Nuclear Protein to the DNA
The antigen Ki-67 Ag, regarded as a marker for proliferating cells, was identified as a protein(s) (pKi-67) which can exist free or associated with DNA as evidenced by DNA digestion of cells before or after immunolabeling with Ki-67. The dual nature of this antigen was also supported by reconstituti...
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| Veröffentlicht in: | Experimental cell research 1994-02, Vol.210 (2), p.145-153 |
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| container_title | Experimental cell research |
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| creator | Lopez, F. Belloc, F. Lacombe, F. Dumain, P. Reiffers, J. Bernard, P. Boisseau, M.R. |
| description | The antigen Ki-67 Ag, regarded as a marker for proliferating cells, was identified as a protein(s) (pKi-67) which can exist free or associated with DNA as evidenced by DNA digestion of cells before or after immunolabeling with Ki-67. The dual nature of this antigen was also supported by reconstitution of Ki-67 Ag from purified DNA and nuclear proteins extracted from the K562 cell line. The immunoreactivity of the resulting complexes was examined in solution using Ki-67 and MIB-1 antibodies. The interaction between Ki-67 or MIB-1 antibodies and pKi-67 was enhanced in the presence of undegraded ds DNA, indicating that ds DNA modulates the conformation of pKi-67 and that the altered conformation of pKi-67 is more reactive than the pure protein to both Ki-67 and MIB-1 antibodies. |
| doi_str_mv | 10.1006/excr.1994.1022 |
| format | Article |
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The dual nature of this antigen was also supported by reconstitution of Ki-67 Ag from purified DNA and nuclear proteins extracted from the K562 cell line. The immunoreactivity of the resulting complexes was examined in solution using Ki-67 and MIB-1 antibodies. The interaction between Ki-67 or MIB-1 antibodies and pKi-67 was enhanced in the presence of undegraded ds DNA, indicating that ds DNA modulates the conformation of pKi-67 and that the altered conformation of pKi-67 is more reactive than the pure protein to both Ki-67 and MIB-1 antibodies.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1006/excr.1994.1022</identifier><identifier>PMID: 8299712</identifier><identifier>CODEN: ECREAL</identifier><language>eng</language><publisher>Orlando, FL: Elsevier Inc</publisher><subject>Antibodies - metabolism ; Biological and medical sciences ; Cell cycle, cell proliferation ; Cell Line ; Cell physiology ; Cycloheximide - pharmacology ; DNA, Neoplasm - metabolism ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Gene Expression - drug effects ; Humans ; Immunoblotting ; Ki-67 Antigen ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; Molecular and cellular biology ; Neoplasm Proteins - biosynthesis ; Neoplasm Proteins - immunology ; Neoplasm Proteins - metabolism ; Nuclear Proteins - biosynthesis ; Nuclear Proteins - immunology ; Nuclear Proteins - isolation & purification ; Nuclear Proteins - metabolism ; Nucleic Acid Conformation ; Protein Conformation ; Tumor Cells, Cultured</subject><ispartof>Experimental cell research, 1994-02, Vol.210 (2), p.145-153</ispartof><rights>1994 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-84a4bb4d40174d632c5230f1ed8efd9b4531830e0e4f57391cafc2b2d242a69c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/excr.1994.1022$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3934212$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8299712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lopez, F.</creatorcontrib><creatorcontrib>Belloc, F.</creatorcontrib><creatorcontrib>Lacombe, F.</creatorcontrib><creatorcontrib>Dumain, P.</creatorcontrib><creatorcontrib>Reiffers, J.</creatorcontrib><creatorcontrib>Bernard, P.</creatorcontrib><creatorcontrib>Boisseau, M.R.</creatorcontrib><title>The Labeling of Proliferating Cells by Ki67 and MIB-1 Antibodies Depends on the Binding of a Nuclear Protein to the DNA</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>The antigen Ki-67 Ag, regarded as a marker for proliferating cells, was identified as a protein(s) (pKi-67) which can exist free or associated with DNA as evidenced by DNA digestion of cells before or after immunolabeling with Ki-67. The dual nature of this antigen was also supported by reconstitution of Ki-67 Ag from purified DNA and nuclear proteins extracted from the K562 cell line. The immunoreactivity of the resulting complexes was examined in solution using Ki-67 and MIB-1 antibodies. The interaction between Ki-67 or MIB-1 antibodies and pKi-67 was enhanced in the presence of undegraded ds DNA, indicating that ds DNA modulates the conformation of pKi-67 and that the altered conformation of pKi-67 is more reactive than the pure protein to both Ki-67 and MIB-1 antibodies.</description><subject>Antibodies - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Cycloheximide - pharmacology</subject><subject>DNA, Neoplasm - metabolism</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - drug effects</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Ki-67 Antigen</subject><subject>Leukemia, Myelogenous, Chronic, BCR-ABL Positive</subject><subject>Molecular and cellular biology</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Neoplasm Proteins - immunology</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Nuclear Proteins - biosynthesis</subject><subject>Nuclear Proteins - immunology</subject><subject>Nuclear Proteins - isolation & purification</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nucleic Acid Conformation</subject><subject>Protein Conformation</subject><subject>Tumor Cells, Cultured</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1vEzEQhi1EVdLClRuSD4jbpv7Krn1MUyhV08KhnC2vPQajjR3sDdB_j7dZ9cbJsufxOzMPQm8pWVJC2gv4a_OSKiXqlbEXaEGJIg0TjL1EC0KoaIRk3St0VspPQoiUtD1Fp5Ip1VG2QH8efgDemh6GEL_j5PHXnIbgIZtxetjAMBTcP-Lb0HbYRIfvbi4bitdxDH1yAQq-gj1EV3CKeKxZlyG6Ocrg-4MdwOQpdIRQgfTEXN2vX6MTb4YCb-bzHH379PFh87nZfrm-2ay3jeWtHBspjOh74QShnXAtZ3bFOPEUnATvVC9WnEpOgIDwq44rao23rGeuCjCtsvwcfTjm7nP6dYAy6l0otm5lIqRD0V3LBVOkreDyCNqcSsng9T6HncmPmhI9mdaTaT2Z1pPp-uHdnHzod-Ce8Vltrb-f66ZYM_hsog3lGeOqNn7C5BGDauF3gKyLDRAtuJDBjtql8L8J_gE8MJew</recordid><startdate>19940201</startdate><enddate>19940201</enddate><creator>Lopez, F.</creator><creator>Belloc, F.</creator><creator>Lacombe, F.</creator><creator>Dumain, P.</creator><creator>Reiffers, J.</creator><creator>Bernard, P.</creator><creator>Boisseau, M.R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19940201</creationdate><title>The Labeling of Proliferating Cells by Ki67 and MIB-1 Antibodies Depends on the Binding of a Nuclear Protein to the DNA</title><author>Lopez, F. ; Belloc, F. ; Lacombe, F. ; Dumain, P. ; Reiffers, J. ; Bernard, P. ; Boisseau, M.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-84a4bb4d40174d632c5230f1ed8efd9b4531830e0e4f57391cafc2b2d242a69c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antibodies - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell cycle, cell proliferation</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>Cycloheximide - pharmacology</topic><topic>DNA, Neoplasm - metabolism</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - drug effects</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Ki-67 Antigen</topic><topic>Leukemia, Myelogenous, Chronic, BCR-ABL Positive</topic><topic>Molecular and cellular biology</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Neoplasm Proteins - immunology</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Nuclear Proteins - biosynthesis</topic><topic>Nuclear Proteins - immunology</topic><topic>Nuclear Proteins - isolation & purification</topic><topic>Nuclear Proteins - metabolism</topic><topic>Nucleic Acid Conformation</topic><topic>Protein Conformation</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lopez, F.</creatorcontrib><creatorcontrib>Belloc, F.</creatorcontrib><creatorcontrib>Lacombe, F.</creatorcontrib><creatorcontrib>Dumain, P.</creatorcontrib><creatorcontrib>Reiffers, J.</creatorcontrib><creatorcontrib>Bernard, P.</creatorcontrib><creatorcontrib>Boisseau, M.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lopez, F.</au><au>Belloc, F.</au><au>Lacombe, F.</au><au>Dumain, P.</au><au>Reiffers, J.</au><au>Bernard, P.</au><au>Boisseau, M.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Labeling of Proliferating Cells by Ki67 and MIB-1 Antibodies Depends on the Binding of a Nuclear Protein to the DNA</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1994-02-01</date><risdate>1994</risdate><volume>210</volume><issue>2</issue><spage>145</spage><epage>153</epage><pages>145-153</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>The antigen Ki-67 Ag, regarded as a marker for proliferating cells, was identified as a protein(s) (pKi-67) which can exist free or associated with DNA as evidenced by DNA digestion of cells before or after immunolabeling with Ki-67. The dual nature of this antigen was also supported by reconstitution of Ki-67 Ag from purified DNA and nuclear proteins extracted from the K562 cell line. The immunoreactivity of the resulting complexes was examined in solution using Ki-67 and MIB-1 antibodies. The interaction between Ki-67 or MIB-1 antibodies and pKi-67 was enhanced in the presence of undegraded ds DNA, indicating that ds DNA modulates the conformation of pKi-67 and that the altered conformation of pKi-67 is more reactive than the pure protein to both Ki-67 and MIB-1 antibodies.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>8299712</pmid><doi>10.1006/excr.1994.1022</doi><tpages>9</tpages></addata></record> |
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| ispartof | Experimental cell research, 1994-02, Vol.210 (2), p.145-153 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Antibodies - metabolism Biological and medical sciences Cell cycle, cell proliferation Cell Line Cell physiology Cycloheximide - pharmacology DNA, Neoplasm - metabolism Flow Cytometry Fundamental and applied biological sciences. Psychology Gene Expression - drug effects Humans Immunoblotting Ki-67 Antigen Leukemia, Myelogenous, Chronic, BCR-ABL Positive Molecular and cellular biology Neoplasm Proteins - biosynthesis Neoplasm Proteins - immunology Neoplasm Proteins - metabolism Nuclear Proteins - biosynthesis Nuclear Proteins - immunology Nuclear Proteins - isolation & purification Nuclear Proteins - metabolism Nucleic Acid Conformation Protein Conformation Tumor Cells, Cultured |
| title | The Labeling of Proliferating Cells by Ki67 and MIB-1 Antibodies Depends on the Binding of a Nuclear Protein to the DNA |
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