A rapid membrane based immunobinding assay for the detection of dengue virus in tissue culture

A rapid, simple dot immunoassay (DOTIA) was developed and evaluated for the detection of dengue-1 viral antigen in infected Aedes albopictus C6/36 cells. Dengue virus infected cells were solubilize in sodium dodecyl sulfate (SDS) and the lysate was pressure filtered through a hydrophobic polyvinylid...

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Veröffentlicht in:	Southeast Asian journal of tropical medicine and public health 1993-12, Vol.24 (4), p.742-746
Hauptverfasser:	SIMMONS, M, JENNINGS, G, OPRANDY, J. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Aedes - cytology Animals Antigens, Viral - isolation & purification Arboviroses Biological and medical sciences Culture Techniques Dengue fevers Dengue Virus - immunology Human viral diseases Immunoassay - methods Infectious diseases Medical sciences Tropical medicine Tropical viral diseases Viral diseases Viral Plaque Assay
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