Methods for the isolation of viable gonadotropin-releasing hormone (LRF) neurons
Studies were conducted in order to determine if selected neurons could be isolated from the brain using Sepharose-linked recognition complexes directed against or related to the biosynthetic/neurosecretory product of the desired neuronal population. Immunoreactive LRF neurons were precipitated when...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 1985-03, Vol.6 (2), p.347-351 |
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| container_title | Peptides (New York, N.Y. : 1980) |
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| creator | Melrose, Patricia Ann Knigge, Karl M. |
| description | Studies were conducted in order to determine if selected neurons could be isolated from the brain using Sepharose-linked recognition complexes directed against or related to the biosynthetic/neurosecretory product of the desired neuronal population. Immunoreactive LRF neurons were precipitated when dispersed cells of adult male rats were incubated successively in media containing free LRF antiserum followed by the exposure of LRF bound to Sepharose-4B. The radioimmunoassayable LRF content of the isolated cells was 88% of that contained in fresh frozen tissue of a contemporary group of rats and trypan blue exclusion indicated that at least 85% of the neurons were viable. Furthermore, based on immunocytochemistry and cresyl violet staining in combination with immunocytochemistry, the isolated cell fraction appeared to be free from other types of cells and also exhibited assayable LRF release when challenged with potassium. These results suggest that the neuroendocrine properties of hypothalamic neurons may be exploited in order to isolate viable cells for acute
in vitro experiments. |
| doi_str_mv | 10.1016/0196-9781(85)90061-0 |
| format | Article |
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in vitro experiments.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - cytology</subject><subject>Cell Separation - methods</subject><subject>Cell sorting</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gonadotropin-Releasing Hormone - analysis</subject><subject>Gonadotropin-Releasing Hormone - metabolism</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>LRF</subject><subject>Male</subject><subject>Neurons</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Neuropeptides</subject><subject>Polylysine</subject><subject>Potassium - pharmacology</subject><subject>Radioimmunoassay</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Vertebrates: endocrinology</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo67j6DxRyENk9tFa6k3RyWZDFVWFEET2HfFR2Ij3JmPQs-O_tcYY5eqrD-7xVxUPISwZvGTD5DpiWnR4Vu1LiWgNI1sEjsmJqHDrBpH5MVmfkKXnW2i8A4FyrC3IxKK2Aw4p8-4LzpoRGY6l03iBNrUx2TiXTEulDsm5Cel-yDWWuZZdyV3FC21K-p5tStyUjvVp_v7umGfe15PacPIl2avjiNC_Jz7sPP24_deuvHz_fvl93flBy7qTWLHAIIwQWhBNa9DJy14_CyT5Kx9kSISrHneIMRXRsQOjRDTZ6r_hwSd4c9-5q-b3HNpttah6nyWYs-2ZG2WsY1bCA_Aj6WlqrGM2upq2tfwwDcxBpDpbMwZJRwvwTaWCpvTrt37sthnPpZG7JX59y27ydYrXZp3bGlAAYxeH6zRHDxcVDwmqaT5g9hlTRzyaU9P8__gI2KI7B</recordid><startdate>198503</startdate><enddate>198503</enddate><creator>Melrose, Patricia Ann</creator><creator>Knigge, Karl M.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198503</creationdate><title>Methods for the isolation of viable gonadotropin-releasing hormone (LRF) neurons</title><author>Melrose, Patricia Ann ; Knigge, Karl M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-6991d40d70d1d5b59526f4b275b62f6b41d70ee8b4b841e5fb13e02eb3afcc843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - cytology</topic><topic>Cell Separation - methods</topic><topic>Cell sorting</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gonadotropin-Releasing Hormone - analysis</topic><topic>Gonadotropin-Releasing Hormone - metabolism</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>LRF</topic><topic>Male</topic><topic>Neurons</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Neuropeptides</topic><topic>Polylysine</topic><topic>Potassium - pharmacology</topic><topic>Radioimmunoassay</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Melrose, Patricia Ann</creatorcontrib><creatorcontrib>Knigge, Karl M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Melrose, Patricia Ann</au><au>Knigge, Karl M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methods for the isolation of viable gonadotropin-releasing hormone (LRF) neurons</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>1985-03</date><risdate>1985</risdate><volume>6</volume><issue>2</issue><spage>347</spage><epage>351</epage><pages>347-351</pages><issn>0196-9781</issn><eissn>1873-5169</eissn><coden>PPTDD5</coden><abstract>Studies were conducted in order to determine if selected neurons could be isolated from the brain using Sepharose-linked recognition complexes directed against or related to the biosynthetic/neurosecretory product of the desired neuronal population. Immunoreactive LRF neurons were precipitated when dispersed cells of adult male rats were incubated successively in media containing free LRF antiserum followed by the exposure of LRF bound to Sepharose-4B. The radioimmunoassayable LRF content of the isolated cells was 88% of that contained in fresh frozen tissue of a contemporary group of rats and trypan blue exclusion indicated that at least 85% of the neurons were viable. Furthermore, based on immunocytochemistry and cresyl violet staining in combination with immunocytochemistry, the isolated cell fraction appeared to be free from other types of cells and also exhibited assayable LRF release when challenged with potassium. These results suggest that the neuroendocrine properties of hypothalamic neurons may be exploited in order to isolate viable cells for acute
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| identifier | ISSN: 0196-9781 |
| ispartof | Peptides (New York, N.Y. : 1980), 1985-03, Vol.6 (2), p.347-351 |
| issn | 0196-9781 1873-5169 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Brain - cytology Cell Separation - methods Cell sorting Fundamental and applied biological sciences. Psychology Gonadotropin-Releasing Hormone - analysis Gonadotropin-Releasing Hormone - metabolism Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis LRF Male Neurons Neurons - cytology Neurons - metabolism Neuropeptides Polylysine Potassium - pharmacology Radioimmunoassay Rats Rats, Inbred Strains Vertebrates: endocrinology |
| title | Methods for the isolation of viable gonadotropin-releasing hormone (LRF) neurons |
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