Preimplantation embryo sexing by polymerase chain reaction amplification of the sry gene on single mouse blastomeres
Accurate and rapid sex determination of preimplantation embryos has great potential both in animal breeding and in human pathology. In the past, sex determination has been accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetit...
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| Veröffentlicht in: | Genetic analysis, techniques and applications techniques and applications, 1993, Vol.10 (6), p.147-149 |
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| container_title | Genetic analysis, techniques and applications |
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| creator | Mulder, Lubbertus C. Sacco, Maria G. Mangiarini, Laura Brown, John Collotta, Angelo Villa, Anna De Giovanni, Anna M. Vezzoni, Paolo Clerici, Libero |
| description | Accurate and rapid sex determination of preimplantation embryos has great potential both in animal breeding and in human pathology. In the past, sex determination has been accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetitive sequences. More recently, amplification of the Y-specific single-copy
ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (
sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for
sry amplification were reimplanted to foster mothers, and six of six newborns were male. We conclude that
sry gene amplification can represent a good marker for embryo sex determination. |
| doi_str_mv | 10.1016/1050-3862(93)90010-G |
| format | Article |
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ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (
sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for
sry amplification were reimplanted to foster mothers, and six of six newborns were male. We conclude that
sry gene amplification can represent a good marker for embryo sex determination.</description><identifier>ISSN: 1050-3862</identifier><identifier>DOI: 10.1016/1050-3862(93)90010-G</identifier><identifier>PMID: 8043307</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Blastocyst - classification ; Blastomeres ; DNA-Binding Proteins - genetics ; Embryo Transfer ; Female ; Genetic Markers ; Male ; Mice ; Molecular Sequence Data ; Nuclear Proteins ; Polymerase Chain Reaction ; Sex Determination Analysis - methods ; Sex-Determining Region Y Protein ; Transcription Factors ; Y Chromosome</subject><ispartof>Genetic analysis, techniques and applications, 1993, Vol.10 (6), p.147-149</ispartof><rights>1993</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-f61387a315ed5f95682558dd39acd20d42704098e75957ac717d8b8de9fca013</citedby><cites>FETCH-LOGICAL-c458t-f61387a315ed5f95682558dd39acd20d42704098e75957ac717d8b8de9fca013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8043307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mulder, Lubbertus C.</creatorcontrib><creatorcontrib>Sacco, Maria G.</creatorcontrib><creatorcontrib>Mangiarini, Laura</creatorcontrib><creatorcontrib>Brown, John</creatorcontrib><creatorcontrib>Collotta, Angelo</creatorcontrib><creatorcontrib>Villa, Anna</creatorcontrib><creatorcontrib>De Giovanni, Anna M.</creatorcontrib><creatorcontrib>Vezzoni, Paolo</creatorcontrib><creatorcontrib>Clerici, Libero</creatorcontrib><title>Preimplantation embryo sexing by polymerase chain reaction amplification of the sry gene on single mouse blastomeres</title><title>Genetic analysis, techniques and applications</title><addtitle>Genet Anal Tech Appl</addtitle><description>Accurate and rapid sex determination of preimplantation embryos has great potential both in animal breeding and in human pathology. In the past, sex determination has been accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetitive sequences. More recently, amplification of the Y-specific single-copy
ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (
sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for
sry amplification were reimplanted to foster mothers, and six of six newborns were male. We conclude that
sry gene amplification can represent a good marker for embryo sex determination.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Blastocyst - classification</subject><subject>Blastomeres</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Embryo Transfer</subject><subject>Female</subject><subject>Genetic Markers</subject><subject>Male</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins</subject><subject>Polymerase Chain Reaction</subject><subject>Sex Determination Analysis - methods</subject><subject>Sex-Determining Region Y Protein</subject><subject>Transcription Factors</subject><subject>Y Chromosome</subject><issn>1050-3862</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1r3DAQ1aEh3Wz7D1rQqaQHpyPLsqVLICztJhBID3sXsjROVGxrK3lD_e-r_WCPyWlg5r03M-8R8oXBDQNW_2AgoOCyLq8V_64AGBTrD2Rxbn8kVyn9AeAgKnlJLiVUnEOzINPviH7Y9maczOTDSHFo4xxown9-fKbtTLehnweMJiG1L8aPNKKxB6jJPN95eySGjk4vSFOc6TOOSHMrZYke6RB2mdz2Jk0hK2H6RC460yf8fKpLsvn1c7O6Lx6f1g-ru8fCVkJORVczLhvDmUAnOiVqWQohnePKWFeCq8oGKlASG6FEY2zDGidb6VB11gDjS_LtKLuN4e8O06QHnyz2-VnMJ-mmLqXKO94FslqBZLXIwOoItDGkFLHT2-gHE2fNQO-D0HvH9d5xrbg-BKHXmfb1pL9rB3Rn0imFPL89zjGb8eox6mQ9jhadj2gn7YJ_e8F_YbybXA</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Mulder, Lubbertus C.</creator><creator>Sacco, Maria G.</creator><creator>Mangiarini, Laura</creator><creator>Brown, John</creator><creator>Collotta, Angelo</creator><creator>Villa, Anna</creator><creator>De Giovanni, Anna M.</creator><creator>Vezzoni, Paolo</creator><creator>Clerici, Libero</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Preimplantation embryo sexing by polymerase chain reaction amplification of the sry gene on single mouse blastomeres</title><author>Mulder, Lubbertus C. ; 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In the past, sex determination has been accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetitive sequences. More recently, amplification of the Y-specific single-copy
ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (
sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for
sry amplification were reimplanted to foster mothers, and six of six newborns were male. We conclude that
sry gene amplification can represent a good marker for embryo sex determination.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8043307</pmid><doi>10.1016/1050-3862(93)90010-G</doi><tpages>3</tpages></addata></record> |
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| ispartof | Genetic analysis, techniques and applications, 1993, Vol.10 (6), p.147-149 |
| issn | 1050-3862 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Base Sequence Blastocyst - classification Blastomeres DNA-Binding Proteins - genetics Embryo Transfer Female Genetic Markers Male Mice Molecular Sequence Data Nuclear Proteins Polymerase Chain Reaction Sex Determination Analysis - methods Sex-Determining Region Y Protein Transcription Factors Y Chromosome |
| title | Preimplantation embryo sexing by polymerase chain reaction amplification of the sry gene on single mouse blastomeres |
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