Immunoenzymatic staining of viral and chlamydial antigens in cell culture

Methods are described for the conjugation of antibodies with biotin and for the use of these reagents in an immunoperoxidase staining procedure for infected cell cultures. This technique provides a simple, rapid, and specific approach to the identification and characterization of a number of viral a...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 1985-01, Vol.3 (4), p.353-358
Hauptverfasser:	Richman, Douglas D., Cleveland, Patrick H., Wopschall, L.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviridae - immunology Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Monoclonal Antigens, Bacterial - analysis Antigens, Bacterial - immunology Antigens, Viral - analysis Antigens, Viral - immunology Biological and medical sciences Biotin Cell Line Chlamydia Chlamydia trachomatis - immunology Cytomegalovirus - immunology Dogs Epitopes Fundamental and applied biological sciences. Psychology Fundamental immunology Herpesvirus 3, Human - immunology Humans Immunobiology Immunoenzyme Techniques Influenza A virus - immunology Influenza B virus - immunology Miscellaneous Regulatory factors and their cellular receptors Simplexvirus - immunology Staining and Labeling
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Beschreibung
Zusammenfassung:	Methods are described for the conjugation of antibodies with biotin and for the use of these reagents in an immunoperoxidase staining procedure for infected cell cultures. This technique provides a simple, rapid, and specific approach to the identification and characterization of a number of viral and chlamydial isolates in the diagnostic laboratory.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/0732-8893(85)90010-0