Application of in vitro Bromodeoxyuridine Labeling of Oral Cancers
We used immunohistochemical methods to investigate whether in vitro labeling with bromodeoxyuridine (BrdU) of oral cancer tissues is useful in pathological diagnosis. BrdU-positive cells were found around the basal region of the epithelium in normal oral mucosa, in a papilloma specimen and in tissue...
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| Veröffentlicht in: | Journal of Osaka Dental University 1993/10/11, Vol.27(2), pp.113-120 |
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| container_issue | 2 |
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| container_title | Journal of Osaka Dental University |
| container_volume | 27 |
| creator | WATO, Masahiro NISHIKAWA, Tetsunari FUJITA, Atsushi NAGAO, Yutaka TSUJIMOTO, Kengo MATSUOKA, Kayo HORII, Katsuko OKANO, Hakuro TANAKA, Akio |
| description | We used immunohistochemical methods to investigate whether in vitro labeling with bromodeoxyuridine (BrdU) of oral cancer tissues is useful in pathological diagnosis. BrdU-positive cells were found around the basal region of the epithelium in normal oral mucosa, in a papilloma specimen and in tissue with moderate dysplasia. The labeling rates (percentage of BrdU-positive cells, LR) for these cases were 1.2%, 6.9% and 7.5%, respectively. In well-differentiated carcinoma, more layers of BrdU positive cells were observed from the basal layer toward the surface. The LR in this region was 13.9-17.0%. In vitro BrdU labeling of oral tumors may be useful in pathological diagnosis, since the LR is lowest in normal tissues, higher in benign tumors, and highest in malignant tumor tissues. |
| doi_str_mv | 10.18905/jodu.27.2_113 |
| format | Article |
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BrdU-positive cells were found around the basal region of the epithelium in normal oral mucosa, in a papilloma specimen and in tissue with moderate dysplasia. The labeling rates (percentage of BrdU-positive cells, LR) for these cases were 1.2%, 6.9% and 7.5%, respectively. In well-differentiated carcinoma, more layers of BrdU positive cells were observed from the basal layer toward the surface. The LR in this region was 13.9-17.0%. In vitro BrdU labeling of oral tumors may be useful in pathological diagnosis, since the LR is lowest in normal tissues, higher in benign tumors, and highest in malignant tumor tissues.</description><identifier>ISSN: 0475-2058</identifier><identifier>EISSN: 2189-6488</identifier><identifier>DOI: 10.18905/jodu.27.2_113</identifier><identifier>PMID: 8182503</identifier><language>eng</language><publisher>Japan: Osaka Odontological Society</publisher><subject>Bromodeoxyuridine ; Carcinoma, Squamous Cell - diagnosis ; Cell Division ; Cell kinetics ; Cell Transformation, Neoplastic - pathology ; Dentistry ; Epithelium - pathology ; Humans ; Immunoenzyme Techniques ; Immunohistochemistry ; Mouth Neoplasms - diagnosis ; Oral cancers ; Papilloma - diagnosis ; S Phase ; Tumor Cells, Cultured</subject><ispartof>Journal of Osaka Dental University, 1993/10/11, Vol.27(2), pp.113-120</ispartof><rights>1993 Osaka Odontological Society</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8182503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WATO, Masahiro</creatorcontrib><creatorcontrib>NISHIKAWA, Tetsunari</creatorcontrib><creatorcontrib>FUJITA, Atsushi</creatorcontrib><creatorcontrib>NAGAO, Yutaka</creatorcontrib><creatorcontrib>TSUJIMOTO, Kengo</creatorcontrib><creatorcontrib>MATSUOKA, Kayo</creatorcontrib><creatorcontrib>HORII, Katsuko</creatorcontrib><creatorcontrib>OKANO, Hakuro</creatorcontrib><creatorcontrib>TANAKA, Akio</creatorcontrib><title>Application of in vitro Bromodeoxyuridine Labeling of Oral Cancers</title><title>Journal of Osaka Dental University</title><addtitle>JODU</addtitle><description>We used immunohistochemical methods to investigate whether in vitro labeling with bromodeoxyuridine (BrdU) of oral cancer tissues is useful in pathological diagnosis. BrdU-positive cells were found around the basal region of the epithelium in normal oral mucosa, in a papilloma specimen and in tissue with moderate dysplasia. The labeling rates (percentage of BrdU-positive cells, LR) for these cases were 1.2%, 6.9% and 7.5%, respectively. In well-differentiated carcinoma, more layers of BrdU positive cells were observed from the basal layer toward the surface. The LR in this region was 13.9-17.0%. In vitro BrdU labeling of oral tumors may be useful in pathological diagnosis, since the LR is lowest in normal tissues, higher in benign tumors, and highest in malignant tumor tissues.</description><subject>Bromodeoxyuridine</subject><subject>Carcinoma, Squamous Cell - diagnosis</subject><subject>Cell Division</subject><subject>Cell kinetics</subject><subject>Cell Transformation, Neoplastic - pathology</subject><subject>Dentistry</subject><subject>Epithelium - pathology</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Immunohistochemistry</subject><subject>Mouth Neoplasms - diagnosis</subject><subject>Oral cancers</subject><subject>Papilloma - diagnosis</subject><subject>S Phase</subject><subject>Tumor Cells, Cultured</subject><issn>0475-2058</issn><issn>2189-6488</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kDFPwzAQRi0EKqWwsiFlYkuxz07sjG0FFKjUBWbLSZziKLGDnSD670nVqjfcSfeeTvoOoXuC50RkOHmqXTnMgc9BEkIv0BTGdZwyIS7RFDOexIATcY1uQqgxZplg2QRNBBGQYDpFy0XXNaZQvXE2clVkbPRreu-ipXetK7X72w_elMbqaKNy3Ri7O2hbr5popWyhfbhFV5Vqgr47zRn6enn-XK3jzfb1bbXYxDWloo8LnGFFKtAZz6uCCQYVSbiCpEoZzxnJuea0zBhLGCgMLKUplJCDGMEYgdAZejze7bz7GXToZWtCoZtGWe2GIHkKnKUYj-LDSRzyVpey86ZVfi9PoUe-PvI69Gqnz1z53hSNloePSuASDu3jHY9FAFNg4qwU38pLbek_klhxIg</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>WATO, Masahiro</creator><creator>NISHIKAWA, Tetsunari</creator><creator>FUJITA, Atsushi</creator><creator>NAGAO, Yutaka</creator><creator>TSUJIMOTO, Kengo</creator><creator>MATSUOKA, Kayo</creator><creator>HORII, Katsuko</creator><creator>OKANO, Hakuro</creator><creator>TANAKA, Akio</creator><general>Osaka Odontological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Application of in vitro Bromodeoxyuridine Labeling of Oral Cancers</title><author>WATO, Masahiro ; NISHIKAWA, Tetsunari ; FUJITA, Atsushi ; NAGAO, Yutaka ; TSUJIMOTO, Kengo ; MATSUOKA, Kayo ; HORII, Katsuko ; OKANO, Hakuro ; TANAKA, Akio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j338t-c090a1f2e97bfc4842f157a25f647b41b7e73d944542a0246362d2b28b7e20513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bromodeoxyuridine</topic><topic>Carcinoma, Squamous Cell - diagnosis</topic><topic>Cell Division</topic><topic>Cell kinetics</topic><topic>Cell Transformation, Neoplastic - pathology</topic><topic>Dentistry</topic><topic>Epithelium - pathology</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Immunohistochemistry</topic><topic>Mouth Neoplasms - diagnosis</topic><topic>Oral cancers</topic><topic>Papilloma - diagnosis</topic><topic>S Phase</topic><topic>Tumor Cells, Cultured</topic><toplevel>online_resources</toplevel><creatorcontrib>WATO, Masahiro</creatorcontrib><creatorcontrib>NISHIKAWA, Tetsunari</creatorcontrib><creatorcontrib>FUJITA, Atsushi</creatorcontrib><creatorcontrib>NAGAO, Yutaka</creatorcontrib><creatorcontrib>TSUJIMOTO, Kengo</creatorcontrib><creatorcontrib>MATSUOKA, Kayo</creatorcontrib><creatorcontrib>HORII, Katsuko</creatorcontrib><creatorcontrib>OKANO, Hakuro</creatorcontrib><creatorcontrib>TANAKA, Akio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Osaka Dental University</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WATO, Masahiro</au><au>NISHIKAWA, Tetsunari</au><au>FUJITA, Atsushi</au><au>NAGAO, Yutaka</au><au>TSUJIMOTO, Kengo</au><au>MATSUOKA, Kayo</au><au>HORII, Katsuko</au><au>OKANO, Hakuro</au><au>TANAKA, Akio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of in vitro Bromodeoxyuridine Labeling of Oral Cancers</atitle><jtitle>Journal of Osaka Dental University</jtitle><addtitle>JODU</addtitle><date>1993</date><risdate>1993</risdate><volume>27</volume><issue>2</issue><spage>113</spage><epage>120</epage><pages>113-120</pages><issn>0475-2058</issn><eissn>2189-6488</eissn><abstract>We used immunohistochemical methods to investigate whether in vitro labeling with bromodeoxyuridine (BrdU) of oral cancer tissues is useful in pathological diagnosis. BrdU-positive cells were found around the basal region of the epithelium in normal oral mucosa, in a papilloma specimen and in tissue with moderate dysplasia. The labeling rates (percentage of BrdU-positive cells, LR) for these cases were 1.2%, 6.9% and 7.5%, respectively. In well-differentiated carcinoma, more layers of BrdU positive cells were observed from the basal layer toward the surface. The LR in this region was 13.9-17.0%. In vitro BrdU labeling of oral tumors may be useful in pathological diagnosis, since the LR is lowest in normal tissues, higher in benign tumors, and highest in malignant tumor tissues.</abstract><cop>Japan</cop><pub>Osaka Odontological Society</pub><pmid>8182503</pmid><doi>10.18905/jodu.27.2_113</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bromodeoxyuridine Carcinoma, Squamous Cell - diagnosis Cell Division Cell kinetics Cell Transformation, Neoplastic - pathology Dentistry Epithelium - pathology Humans Immunoenzyme Techniques Immunohistochemistry Mouth Neoplasms - diagnosis Oral cancers Papilloma - diagnosis S Phase Tumor Cells, Cultured |
| title | Application of in vitro Bromodeoxyuridine Labeling of Oral Cancers |
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