Direct and quantitative detection of HIV-1 RNA in human plasma with a branched DNA signal amplification assay

To determine the relative effect of sample matrix on the quantitation of HIV RNA in plasma. Two HIV-positive specimens were diluted into five and 10 different HIV-negative plasma samples, respectively. Branched DNA signal amplification technology and reverse-transcriptase polymerase chain reaction w...

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Veröffentlicht in:	AIDS (London) 1993-11, Vol.7, p.S11-S14
Hauptverfasser:	URDEA, M. S, WILBER, J. C, NEUWALD, P, PACHL, C. A, YEGHIAZARIAN, T, TODD, J. A, KERN, D. G, FONG, S.-J, BESEMER, D, HOO, B, SHERIDAN, P. J, KOKKA, R
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Biological and medical sciences DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Gene Amplification HIV Infections - blood HIV Infections - microbiology HIV-1 - genetics HIV-1 - isolation & purification Humans Microbiology Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data RNA, Viral - blood RNA, Viral - genetics Sensitivity and Specificity Techniques used in virology Viremia - blood Viremia - microbiology Virology Virology - methods Virology - statistics & numerical data
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