Direct and quantitative detection of HIV-1 RNA in human plasma with a branched DNA signal amplification assay
To determine the relative effect of sample matrix on the quantitation of HIV RNA in plasma. Two HIV-positive specimens were diluted into five and 10 different HIV-negative plasma samples, respectively. Branched DNA signal amplification technology and reverse-transcriptase polymerase chain reaction w...
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| Veröffentlicht in: | AIDS (London) 1993-11, Vol.7, p.S11-S14 |
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| creator | URDEA, M. S WILBER, J. C NEUWALD, P PACHL, C. A YEGHIAZARIAN, T TODD, J. A KERN, D. G FONG, S.-J BESEMER, D HOO, B SHERIDAN, P. J KOKKA, R |
| description | To determine the relative effect of sample matrix on the quantitation of HIV RNA in plasma.
Two HIV-positive specimens were diluted into five and 10 different HIV-negative plasma samples, respectively. Branched DNA signal amplification technology and reverse-transcriptase polymerase chain reaction were used to measure the viral load.
In one sample the viral load by polymerase chain reaction ranged from undetectable to 1.9 x 10(5) copies/ml, and the branched DNA results ranged from 2.6 x 10(4) to 4.2 x 10(4) HIV RNA equivalent/ml. In the other sample the corresponding figures were 6.3 x 10(4) to 5.5 x 10(5) copies/ml and 5.7 x 10(4) to 7.5 x 10(4) HIV RNA equivalents/ml.
In contrast to reverse-transcriptase polymerase chain reaction the branched DNA signal amplification assay does not require a separate extraction step or enzymatic amplification of the target. Therefore this measurement is less affected by the sample matrix and the signal generated is directly proportional to the viral load. |
| doi_str_mv | 10.1097/00002030-199311002-00004 |
| format | Article |
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Two HIV-positive specimens were diluted into five and 10 different HIV-negative plasma samples, respectively. Branched DNA signal amplification technology and reverse-transcriptase polymerase chain reaction were used to measure the viral load.
In one sample the viral load by polymerase chain reaction ranged from undetectable to 1.9 x 10(5) copies/ml, and the branched DNA results ranged from 2.6 x 10(4) to 4.2 x 10(4) HIV RNA equivalent/ml. In the other sample the corresponding figures were 6.3 x 10(4) to 5.5 x 10(5) copies/ml and 5.7 x 10(4) to 7.5 x 10(4) HIV RNA equivalents/ml.
In contrast to reverse-transcriptase polymerase chain reaction the branched DNA signal amplification assay does not require a separate extraction step or enzymatic amplification of the target. Therefore this measurement is less affected by the sample matrix and the signal generated is directly proportional to the viral load.</description><identifier>ISSN: 0269-9370</identifier><identifier>EISSN: 1473-5571</identifier><identifier>DOI: 10.1097/00002030-199311002-00004</identifier><identifier>PMID: 8161440</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>AIDS/HIV ; Biological and medical sciences ; DNA, Viral - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; HIV Infections - blood ; HIV Infections - microbiology ; HIV-1 - genetics ; HIV-1 - isolation & purification ; Humans ; Microbiology ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - statistics & numerical data ; RNA, Viral - blood ; RNA, Viral - genetics ; Sensitivity and Specificity ; Techniques used in virology ; Viremia - blood ; Viremia - microbiology ; Virology ; Virology - methods ; Virology - statistics & numerical data</subject><ispartof>AIDS (London), 1993-11, Vol.7, p.S11-S14</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-69a9137a69cdaf6af594bfe221381d2f1b44b092ed8ad72bc8017ac70353557f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,23909,23910,25118,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4036553$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8161440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>URDEA, M. S</creatorcontrib><creatorcontrib>WILBER, J. C</creatorcontrib><creatorcontrib>NEUWALD, P</creatorcontrib><creatorcontrib>PACHL, C. A</creatorcontrib><creatorcontrib>YEGHIAZARIAN, T</creatorcontrib><creatorcontrib>TODD, J. A</creatorcontrib><creatorcontrib>KERN, D. G</creatorcontrib><creatorcontrib>FONG, S.-J</creatorcontrib><creatorcontrib>BESEMER, D</creatorcontrib><creatorcontrib>HOO, B</creatorcontrib><creatorcontrib>SHERIDAN, P. J</creatorcontrib><creatorcontrib>KOKKA, R</creatorcontrib><title>Direct and quantitative detection of HIV-1 RNA in human plasma with a branched DNA signal amplification assay</title><title>AIDS (London)</title><addtitle>AIDS</addtitle><description>To determine the relative effect of sample matrix on the quantitation of HIV RNA in plasma.
Two HIV-positive specimens were diluted into five and 10 different HIV-negative plasma samples, respectively. Branched DNA signal amplification technology and reverse-transcriptase polymerase chain reaction were used to measure the viral load.
In one sample the viral load by polymerase chain reaction ranged from undetectable to 1.9 x 10(5) copies/ml, and the branched DNA results ranged from 2.6 x 10(4) to 4.2 x 10(4) HIV RNA equivalent/ml. In the other sample the corresponding figures were 6.3 x 10(4) to 5.5 x 10(5) copies/ml and 5.7 x 10(4) to 7.5 x 10(4) HIV RNA equivalents/ml.
In contrast to reverse-transcriptase polymerase chain reaction the branched DNA signal amplification assay does not require a separate extraction step or enzymatic amplification of the target. Therefore this measurement is less affected by the sample matrix and the signal generated is directly proportional to the viral load.</description><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>DNA, Viral - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - microbiology</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - isolation & purification</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - statistics & numerical data</subject><subject>RNA, Viral - blood</subject><subject>RNA, Viral - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Techniques used in virology</subject><subject>Viremia - blood</subject><subject>Viremia - microbiology</subject><subject>Virology</subject><subject>Virology - methods</subject><subject>Virology - statistics & numerical data</subject><issn>0269-9370</issn><issn>1473-5571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kNtKxDAQhoMo63p4BCEX4l0106RNcymeVhAFUW_LNE3cSE82rbJvb3a3bm5C_vlmhnyEUGCXwJS8YuHEjLMIlOIA4RGtI7FH5iAkj5JEwj6ZszhVkeKSHZIj778CkbAsm5FZBikIweakvnW90QPFpqTfIzaDG3BwP4aWZgi5axvaWrp4_IiAvj5fU9fQ5VhjQ7sKfY301w1LirTosdFLU9LbwHj32WBFse4qZ53GzRT0Hlcn5MBi5c3pdB-T9_u7t5tF9PTy8Hhz_RRpLmCIUoUKuMRU6RJtijZRorAmjoFnUMYWCiEKpmJTZljKuNAZA4laMp7w8HPLj8nFdm7Xt9-j8UNeO69NVWFj2tHnMo1l0CYCmG1B3bfe98bmXe9q7Fc5sHxtOv83ne9Mb6J169m0YyxqU-4aJ7Whfj7V0Wus7NqQ8zssEGmScP4HOaKE6w</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>URDEA, M. S</creator><creator>WILBER, J. C</creator><creator>NEUWALD, P</creator><creator>PACHL, C. A</creator><creator>YEGHIAZARIAN, T</creator><creator>TODD, J. A</creator><creator>KERN, D. G</creator><creator>FONG, S.-J</creator><creator>BESEMER, D</creator><creator>HOO, B</creator><creator>SHERIDAN, P. J</creator><creator>KOKKA, R</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931101</creationdate><title>Direct and quantitative detection of HIV-1 RNA in human plasma with a branched DNA signal amplification assay</title><author>URDEA, M. S ; WILBER, J. C ; NEUWALD, P ; PACHL, C. A ; YEGHIAZARIAN, T ; TODD, J. A ; KERN, D. G ; FONG, S.-J ; BESEMER, D ; HOO, B ; SHERIDAN, P. J ; KOKKA, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-69a9137a69cdaf6af594bfe221381d2f1b44b092ed8ad72bc8017ac70353557f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>DNA, Viral - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Amplification</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - microbiology</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - isolation & purification</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - statistics & numerical data</topic><topic>RNA, Viral - blood</topic><topic>RNA, Viral - genetics</topic><topic>Sensitivity and Specificity</topic><topic>Techniques used in virology</topic><topic>Viremia - blood</topic><topic>Viremia - microbiology</topic><topic>Virology</topic><topic>Virology - methods</topic><topic>Virology - statistics & numerical data</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>URDEA, M. S</creatorcontrib><creatorcontrib>WILBER, J. C</creatorcontrib><creatorcontrib>NEUWALD, P</creatorcontrib><creatorcontrib>PACHL, C. A</creatorcontrib><creatorcontrib>YEGHIAZARIAN, T</creatorcontrib><creatorcontrib>TODD, J. A</creatorcontrib><creatorcontrib>KERN, D. G</creatorcontrib><creatorcontrib>FONG, S.-J</creatorcontrib><creatorcontrib>BESEMER, D</creatorcontrib><creatorcontrib>HOO, B</creatorcontrib><creatorcontrib>SHERIDAN, P. J</creatorcontrib><creatorcontrib>KOKKA, R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>URDEA, M. S</au><au>WILBER, J. C</au><au>NEUWALD, P</au><au>PACHL, C. A</au><au>YEGHIAZARIAN, T</au><au>TODD, J. A</au><au>KERN, D. G</au><au>FONG, S.-J</au><au>BESEMER, D</au><au>HOO, B</au><au>SHERIDAN, P. J</au><au>KOKKA, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct and quantitative detection of HIV-1 RNA in human plasma with a branched DNA signal amplification assay</atitle><jtitle>AIDS (London)</jtitle><addtitle>AIDS</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>7</volume><spage>S11</spage><epage>S14</epage><pages>S11-S14</pages><issn>0269-9370</issn><eissn>1473-5571</eissn><abstract>To determine the relative effect of sample matrix on the quantitation of HIV RNA in plasma.
Two HIV-positive specimens were diluted into five and 10 different HIV-negative plasma samples, respectively. Branched DNA signal amplification technology and reverse-transcriptase polymerase chain reaction were used to measure the viral load.
In one sample the viral load by polymerase chain reaction ranged from undetectable to 1.9 x 10(5) copies/ml, and the branched DNA results ranged from 2.6 x 10(4) to 4.2 x 10(4) HIV RNA equivalent/ml. In the other sample the corresponding figures were 6.3 x 10(4) to 5.5 x 10(5) copies/ml and 5.7 x 10(4) to 7.5 x 10(4) HIV RNA equivalents/ml.
In contrast to reverse-transcriptase polymerase chain reaction the branched DNA signal amplification assay does not require a separate extraction step or enzymatic amplification of the target. Therefore this measurement is less affected by the sample matrix and the signal generated is directly proportional to the viral load.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>8161440</pmid><doi>10.1097/00002030-199311002-00004</doi></addata></record> |
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| language | eng |
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| subjects | AIDS/HIV Biological and medical sciences DNA, Viral - genetics Fundamental and applied biological sciences. Psychology Gene Amplification HIV Infections - blood HIV Infections - microbiology HIV-1 - genetics HIV-1 - isolation & purification Humans Microbiology Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data RNA, Viral - blood RNA, Viral - genetics Sensitivity and Specificity Techniques used in virology Viremia - blood Viremia - microbiology Virology Virology - methods Virology - statistics & numerical data |
| title | Direct and quantitative detection of HIV-1 RNA in human plasma with a branched DNA signal amplification assay |
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