Inhibition of Cell Growth by Bafilomycin A1, a Selective Inhibitor of Vacuolar H+-ATPase
Bafilomycin A1, a potent selective inhibitor of vacuolar H+-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A1for 50%...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 1993-11, Vol.29A (11), p.862-866 |
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| container_issue | 11 |
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| container_title | In vitro cellular & developmental biology. Animal |
| container_volume | 29A |
| creator | Ohkuma, Shoji Sakae Shimizu Masahiko Noto Yoshimichi Sai Kuninori Kinoshita Hiro-Omi Tamura |
| description | Bafilomycin A1, a potent selective inhibitor of vacuolar H+-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A1for 50% inhibition of cell growth ranged from 10 to 50 nM. The dose response was nearly parallel with that of the bafilomycin$A_1-induced$lysosomal pH increase. The degree of pH increase for growth inhibition produced by bafilomycin A1was similar to that produced by NH4Clin which little difference was recognized in effect among cell types. |
| doi_str_mv | 10.1007/BF02631364 |
| format | Article |
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The concentration of bafilomycin A1for 50% inhibition of cell growth ranged from 10 to 50 nM. The dose response was nearly parallel with that of the bafilomycin$A_1-induced$lysosomal pH increase. 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The degree of pH increase for growth inhibition produced by bafilomycin A1was similar to that produced by NH4Clin which little difference was recognized in effect among cell types.</description><subject>3T3 Cells</subject><subject>Ammonium Chloride - pharmacology</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Cell culture techniques</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cell Line, Transformed</subject><subject>Cell lines</subject><subject>Cells</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cultured cells</subject><subject>Fibroblasts</subject><subject>Growth, Differentiation, and Senescence</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lysosomes - drug effects</subject><subject>Lysosomes - metabolism</subject><subject>Macrolides</subject><subject>Mice</subject><subject>NIH 3T3 cells</subject><subject>PC12 Cells</subject><subject>Proton-Translocating ATPases - antagonists & inhibitors</subject><subject>Vacuoles - drug effects</subject><subject>Vacuoles - enzymology</subject><issn>1071-2690</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9LwzAUx4Moc04vnhVy8qBWkzRJm-M23A8YKDhlt5KkKctoF01aZf-9HSvzXd6D7_f7eO8DwDVGTxih5Hk0QYTHOOb0BPQxo3GUIL46bWeU4Ihwgc7BRQgb1JbAvAd6KeaJQKQPVvPt2ipbW7eFroBjU5Zw6t1vvYZqB0eysKWrdtpu4RA_QgnfTWl0bX8M7ILO73OfUjeulB7OHqLh8k0GcwnOClkGc9X1AfiYvCzHs2jxOp2Ph4tIE5zWEUtYSgVROs2llIamkvHCxJrQNC60yI0yIleScpYiiXPFkxzhmCIjJOFMsXgA7g57v7z7bkyos8oG3b4ht8Y1IUs44ZThvfH-YNTeheBNkX15W0m_yzDK9hizf4yt-bbb2qjK5Edrx63Vbw76JrQEjjIlguL28j9dmXSn</recordid><startdate>199311</startdate><enddate>199311</enddate><creator>Ohkuma, Shoji</creator><creator>Sakae Shimizu</creator><creator>Masahiko Noto</creator><creator>Yoshimichi Sai</creator><creator>Kuninori Kinoshita</creator><creator>Hiro-Omi Tamura</creator><general>Tissue Culture Association, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199311</creationdate><title>Inhibition of Cell Growth by Bafilomycin A1, a Selective Inhibitor of Vacuolar H+-ATPase</title><author>Ohkuma, Shoji ; Sakae Shimizu ; Masahiko Noto ; Yoshimichi Sai ; Kuninori Kinoshita ; Hiro-Omi Tamura</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c218t-5758492bc8daaae48a56fe3c2483fc9debe9dba46580a1db67d01340e9a265b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>3T3 Cells</topic><topic>Ammonium Chloride - pharmacology</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Cell culture techniques</topic><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>Cell Line, Transformed</topic><topic>Cell lines</topic><topic>Cells</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cultured cells</topic><topic>Fibroblasts</topic><topic>Growth, Differentiation, and Senescence</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lysosomes - drug effects</topic><topic>Lysosomes - metabolism</topic><topic>Macrolides</topic><topic>Mice</topic><topic>NIH 3T3 cells</topic><topic>PC12 Cells</topic><topic>Proton-Translocating ATPases - antagonists & inhibitors</topic><topic>Vacuoles - drug effects</topic><topic>Vacuoles - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohkuma, Shoji</creatorcontrib><creatorcontrib>Sakae Shimizu</creatorcontrib><creatorcontrib>Masahiko Noto</creatorcontrib><creatorcontrib>Yoshimichi Sai</creatorcontrib><creatorcontrib>Kuninori Kinoshita</creatorcontrib><creatorcontrib>Hiro-Omi Tamura</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In vitro cellular & developmental biology. 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Animal</jtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>1993-11</date><risdate>1993</risdate><volume>29A</volume><issue>11</issue><spage>862</spage><epage>866</epage><pages>862-866</pages><issn>1071-2690</issn><eissn>1543-706X</eissn><abstract>Bafilomycin A1, a potent selective inhibitor of vacuolar H+-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A1for 50% inhibition of cell growth ranged from 10 to 50 nM. The dose response was nearly parallel with that of the bafilomycin$A_1-induced$lysosomal pH increase. 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| ispartof | In vitro cellular & developmental biology. Animal, 1993-11, Vol.29A (11), p.862-866 |
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| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; SpringerLink Journals - AutoHoldings |
| subjects | 3T3 Cells Ammonium Chloride - pharmacology Animals Anti-Bacterial Agents - pharmacology Cell culture techniques Cell Division - drug effects Cell growth Cell Line, Transformed Cell lines Cells CHO Cells Cricetinae Cultured cells Fibroblasts Growth, Differentiation, and Senescence HeLa Cells Humans Hydrogen-Ion Concentration Lysosomes - drug effects Lysosomes - metabolism Macrolides Mice NIH 3T3 cells PC12 Cells Proton-Translocating ATPases - antagonists & inhibitors Vacuoles - drug effects Vacuoles - enzymology |
| title | Inhibition of Cell Growth by Bafilomycin A1, a Selective Inhibitor of Vacuolar H+-ATPase |
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