The in vitro response of turkey lymphocytes to steroid hormones
The in vitro mitogen response of whole blood turkey lymphocytes to various concentrations of steroid hormones was evaluated. Corticosterone (COS) at concentrations between 1 and 80 ng/ml significantly suppressed the proliferative response (3H-thymidine incorporation) to phytohemagglutinin (PHA) and...
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| Veröffentlicht in: | Avian diseases 1985-04, Vol.29 (2), p.373-383 |
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| creator | Redig, P.T Dunnette, J.L Mauro, L Sivanandan, V Markham, F |
| description | The in vitro mitogen response of whole blood turkey lymphocytes to various concentrations of steroid hormones was evaluated. Corticosterone (COS) at concentrations between 1 and 80 ng/ml significantly suppressed the proliferative response (3H-thymidine incorporation) to phytohemagglutinin (PHA) and concanavalin A (ConA). Non-mitogen-stimulated (NMS) cells were suppressed at concentrations of COS above 5 ng/ml. Progesterone significantly suppressed NMS cells at concentrations of 80 ng/ml, PHA-stimulated cells at concentrations of 500 ng/ml, and ConA-stimulated cells at concentrations of 1000 ng/ml. β-Estradiol enhanced the response of NMS cells at concentrations of 500 ng/ml, had no effect on PHA-stimulated cells, and suppressed the response of ConA-stimulated cells at concentrations greater than 500 ng/ml. Testosterone affected only the ConA response, causing suppression at concentrations above 2000 ng/ml. Corticosterone and progesterone caused 80 and 95% suppression, respectively, of the proliferative response to ConA when compared with non-hormone-treated cells. The possible implications of steroid hormone-induced immunosuppression in the pathogenesis of aspergillosis is discussed. /// Se evaluó la respuesta mitogénica in vitro de linfocitos de sangre entera de pavo a varias concentraciones de hormonas esteroides. La corticosterona a concentraciones entre 1 y 80 ng/ml suprimió significativamente la respuesta proliferativa (incorporación de3H-timidina) a la fitohemaglutinina (FHA) y concanavalina A (ConA). Células no estimuladas con mitógeno fueron suprimidas con concentraciones de corticosterona de más de 5 ng/ml. La progesterona suprimió significativamente a las células no estimuladas con mitógeno a concentraciones de ≥ 80 ng/ml; a las células estimuladas con FHA a concentraciones de ≥ 500 ng/ml y a las células estimuladas con ConA a concentraciones de ≥ 1000 ng/ml. El β-estradiol realzó la respuesta de células no estimuladas a concentraciones de ≥ 500 ng/ml, no tuvo ningún efecto en células estimuladas con FHA y suprimió la respuesta de células estimuladas con ConA a concentraciones de ≥500 ng/ml. La testosterona afectó solamente la respuesta con ConA, causando una supresión a concentraciones de ≥ 2000 ng/ml. La corticosterona y la progesterona causaron una supresión de 80 y 95%, respectivamente, en la respuesta proliferativa a la ConA, en comparación con células no tratadas con hormonas. Se discuten las posibles implicaciones de la inmunosupresión ind |
| doi_str_mv | 10.2307/1590498 |
| format | Article |
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Corticosterone (COS) at concentrations between 1 and 80 ng/ml significantly suppressed the proliferative response (3H-thymidine incorporation) to phytohemagglutinin (PHA) and concanavalin A (ConA). Non-mitogen-stimulated (NMS) cells were suppressed at concentrations of COS above 5 ng/ml. Progesterone significantly suppressed NMS cells at concentrations of 80 ng/ml, PHA-stimulated cells at concentrations of 500 ng/ml, and ConA-stimulated cells at concentrations of 1000 ng/ml. β-Estradiol enhanced the response of NMS cells at concentrations of 500 ng/ml, had no effect on PHA-stimulated cells, and suppressed the response of ConA-stimulated cells at concentrations greater than 500 ng/ml. Testosterone affected only the ConA response, causing suppression at concentrations above 2000 ng/ml. Corticosterone and progesterone caused 80 and 95% suppression, respectively, of the proliferative response to ConA when compared with non-hormone-treated cells. The possible implications of steroid hormone-induced immunosuppression in the pathogenesis of aspergillosis is discussed. /// Se evaluó la respuesta mitogénica in vitro de linfocitos de sangre entera de pavo a varias concentraciones de hormonas esteroides. La corticosterona a concentraciones entre 1 y 80 ng/ml suprimió significativamente la respuesta proliferativa (incorporación de3H-timidina) a la fitohemaglutinina (FHA) y concanavalina A (ConA). Células no estimuladas con mitógeno fueron suprimidas con concentraciones de corticosterona de más de 5 ng/ml. La progesterona suprimió significativamente a las células no estimuladas con mitógeno a concentraciones de ≥ 80 ng/ml; a las células estimuladas con FHA a concentraciones de ≥ 500 ng/ml y a las células estimuladas con ConA a concentraciones de ≥ 1000 ng/ml. El β-estradiol realzó la respuesta de células no estimuladas a concentraciones de ≥ 500 ng/ml, no tuvo ningún efecto en células estimuladas con FHA y suprimió la respuesta de células estimuladas con ConA a concentraciones de ≥500 ng/ml. La testosterona afectó solamente la respuesta con ConA, causando una supresión a concentraciones de ≥ 2000 ng/ml. La corticosterona y la progesterona causaron una supresión de 80 y 95%, respectivamente, en la respuesta proliferativa a la ConA, en comparación con células no tratadas con hormonas. Se discuten las posibles implicaciones de la inmunosupresión inducida por hormonas esteroides en la patogenesis de la aspergilosis.</description><identifier>ISSN: 0005-2086</identifier><identifier>EISSN: 1938-4351</identifier><identifier>DOI: 10.2307/1590498</identifier><identifier>PMID: 4026731</identifier><language>eng</language><publisher>United States: American Association of Avian Pathologists, Inc</publisher><subject>Animals ; Aspergillosis ; ASPERGILLUS ; Blood ; Concanavalin A - pharmacology ; Corticosterone ; Corticosterone - pharmacology ; DINDON ; ENFERMEDADES FUNGOSAS ; Estradiol - pharmacology ; HORMONAS ESTEROIDES ; HORMONE STEROIDIQUE ; Hormones ; Hormones - pharmacology ; In Vitro Techniques ; LINFOCITOS ; LYMPHOCYTE ; Lymphocyte Activation - drug effects ; LYMPHOCYTES ; Lymphocytes - drug effects ; Lymphocytes - immunology ; Male ; Mitogens ; MYCOSE ; MYCOSES ; PAVO ; Phytohemagglutinins - pharmacology ; Progesterone - pharmacology ; STEROID HORMONES ; Steroids ; Testosterone ; Testosterone - pharmacology ; TURKEYS ; Turkeys - immunology</subject><ispartof>Avian diseases, 1985-04, Vol.29 (2), p.373-383</ispartof><rights>Copyright 1985 The American Association of Avian Pathologists, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c239t-5ea97454730beb44c628147a3152d8318fd50de8ee8af451f8bffa0aacc0514f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1590498$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1590498$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,800,27905,27906,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4026731$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Redig, P.T</creatorcontrib><creatorcontrib>Dunnette, J.L</creatorcontrib><creatorcontrib>Mauro, L</creatorcontrib><creatorcontrib>Sivanandan, V</creatorcontrib><creatorcontrib>Markham, F</creatorcontrib><title>The in vitro response of turkey lymphocytes to steroid hormones</title><title>Avian diseases</title><addtitle>Avian Dis</addtitle><description>The in vitro mitogen response of whole blood turkey lymphocytes to various concentrations of steroid hormones was evaluated. Corticosterone (COS) at concentrations between 1 and 80 ng/ml significantly suppressed the proliferative response (3H-thymidine incorporation) to phytohemagglutinin (PHA) and concanavalin A (ConA). Non-mitogen-stimulated (NMS) cells were suppressed at concentrations of COS above 5 ng/ml. Progesterone significantly suppressed NMS cells at concentrations of 80 ng/ml, PHA-stimulated cells at concentrations of 500 ng/ml, and ConA-stimulated cells at concentrations of 1000 ng/ml. β-Estradiol enhanced the response of NMS cells at concentrations of 500 ng/ml, had no effect on PHA-stimulated cells, and suppressed the response of ConA-stimulated cells at concentrations greater than 500 ng/ml. Testosterone affected only the ConA response, causing suppression at concentrations above 2000 ng/ml. Corticosterone and progesterone caused 80 and 95% suppression, respectively, of the proliferative response to ConA when compared with non-hormone-treated cells. The possible implications of steroid hormone-induced immunosuppression in the pathogenesis of aspergillosis is discussed. /// Se evaluó la respuesta mitogénica in vitro de linfocitos de sangre entera de pavo a varias concentraciones de hormonas esteroides. La corticosterona a concentraciones entre 1 y 80 ng/ml suprimió significativamente la respuesta proliferativa (incorporación de3H-timidina) a la fitohemaglutinina (FHA) y concanavalina A (ConA). Células no estimuladas con mitógeno fueron suprimidas con concentraciones de corticosterona de más de 5 ng/ml. La progesterona suprimió significativamente a las células no estimuladas con mitógeno a concentraciones de ≥ 80 ng/ml; a las células estimuladas con FHA a concentraciones de ≥ 500 ng/ml y a las células estimuladas con ConA a concentraciones de ≥ 1000 ng/ml. El β-estradiol realzó la respuesta de células no estimuladas a concentraciones de ≥ 500 ng/ml, no tuvo ningún efecto en células estimuladas con FHA y suprimió la respuesta de células estimuladas con ConA a concentraciones de ≥500 ng/ml. La testosterona afectó solamente la respuesta con ConA, causando una supresión a concentraciones de ≥ 2000 ng/ml. La corticosterona y la progesterona causaron una supresión de 80 y 95%, respectivamente, en la respuesta proliferativa a la ConA, en comparación con células no tratadas con hormonas. Se discuten las posibles implicaciones de la inmunosupresión inducida por hormonas esteroides en la patogenesis de la aspergilosis.</description><subject>Animals</subject><subject>Aspergillosis</subject><subject>ASPERGILLUS</subject><subject>Blood</subject><subject>Concanavalin A - pharmacology</subject><subject>Corticosterone</subject><subject>Corticosterone - pharmacology</subject><subject>DINDON</subject><subject>ENFERMEDADES FUNGOSAS</subject><subject>Estradiol - pharmacology</subject><subject>HORMONAS ESTEROIDES</subject><subject>HORMONE STEROIDIQUE</subject><subject>Hormones</subject><subject>Hormones - pharmacology</subject><subject>In Vitro Techniques</subject><subject>LINFOCITOS</subject><subject>LYMPHOCYTE</subject><subject>Lymphocyte Activation - drug effects</subject><subject>LYMPHOCYTES</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - immunology</subject><subject>Male</subject><subject>Mitogens</subject><subject>MYCOSE</subject><subject>MYCOSES</subject><subject>PAVO</subject><subject>Phytohemagglutinins - pharmacology</subject><subject>Progesterone - pharmacology</subject><subject>STEROID HORMONES</subject><subject>Steroids</subject><subject>Testosterone</subject><subject>Testosterone - pharmacology</subject><subject>TURKEYS</subject><subject>Turkeys - immunology</subject><issn>0005-2086</issn><issn>1938-4351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9LwzAYhoMoc07xLgg5iJ6qX5qkSU8iw18w8OB2Lmn7xXW2y0wyYf-9kxaPnt7D-_AcHkLOGdymHNQdkzmIXB-QMcu5TgSX7JCMAUAmKejsmJyEsAJgKs9gREYC0kxxNib38yXSZk2_m-gd9Rg2bh2QOkvj1n_ijra7brN01S5ioNHRENG7pqZL5zu3xnBKjqxpA54NOyGLp8f59CWZvT2_Th9mSZXyPCYSTa6EFIpDiaUQVZZqJpThTKa15kzbWkKNGlEbKySzurTWgDFVBZIJyyfkuvduvPvaYohF14QK29as0W1DobI041yqPXjTg5V3IXi0xcY3nfG7gkHxm6oYUu3Jy0G5LTus_7ihzf6_6v9ViM7_o7noMWtcYT58E4rFu85SUHnOfwDVCHZe</recordid><startdate>198504</startdate><enddate>198504</enddate><creator>Redig, P.T</creator><creator>Dunnette, J.L</creator><creator>Mauro, L</creator><creator>Sivanandan, V</creator><creator>Markham, F</creator><general>American Association of Avian Pathologists, Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198504</creationdate><title>The in vitro response of turkey lymphocytes to steroid hormones</title><author>Redig, P.T ; Dunnette, J.L ; Mauro, L ; Sivanandan, V ; Markham, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c239t-5ea97454730beb44c628147a3152d8318fd50de8ee8af451f8bffa0aacc0514f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Aspergillosis</topic><topic>ASPERGILLUS</topic><topic>Blood</topic><topic>Concanavalin A - pharmacology</topic><topic>Corticosterone</topic><topic>Corticosterone - pharmacology</topic><topic>DINDON</topic><topic>ENFERMEDADES FUNGOSAS</topic><topic>Estradiol - pharmacology</topic><topic>HORMONAS ESTEROIDES</topic><topic>HORMONE STEROIDIQUE</topic><topic>Hormones</topic><topic>Hormones - pharmacology</topic><topic>In Vitro Techniques</topic><topic>LINFOCITOS</topic><topic>LYMPHOCYTE</topic><topic>Lymphocyte Activation - drug effects</topic><topic>LYMPHOCYTES</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - immunology</topic><topic>Male</topic><topic>Mitogens</topic><topic>MYCOSE</topic><topic>MYCOSES</topic><topic>PAVO</topic><topic>Phytohemagglutinins - pharmacology</topic><topic>Progesterone - pharmacology</topic><topic>STEROID HORMONES</topic><topic>Steroids</topic><topic>Testosterone</topic><topic>Testosterone - pharmacology</topic><topic>TURKEYS</topic><topic>Turkeys - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Redig, P.T</creatorcontrib><creatorcontrib>Dunnette, J.L</creatorcontrib><creatorcontrib>Mauro, L</creatorcontrib><creatorcontrib>Sivanandan, V</creatorcontrib><creatorcontrib>Markham, F</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Avian diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Redig, P.T</au><au>Dunnette, J.L</au><au>Mauro, L</au><au>Sivanandan, V</au><au>Markham, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The in vitro response of turkey lymphocytes to steroid hormones</atitle><jtitle>Avian diseases</jtitle><addtitle>Avian Dis</addtitle><date>1985-04</date><risdate>1985</risdate><volume>29</volume><issue>2</issue><spage>373</spage><epage>383</epage><pages>373-383</pages><issn>0005-2086</issn><eissn>1938-4351</eissn><abstract>The in vitro mitogen response of whole blood turkey lymphocytes to various concentrations of steroid hormones was evaluated. Corticosterone (COS) at concentrations between 1 and 80 ng/ml significantly suppressed the proliferative response (3H-thymidine incorporation) to phytohemagglutinin (PHA) and concanavalin A (ConA). Non-mitogen-stimulated (NMS) cells were suppressed at concentrations of COS above 5 ng/ml. Progesterone significantly suppressed NMS cells at concentrations of 80 ng/ml, PHA-stimulated cells at concentrations of 500 ng/ml, and ConA-stimulated cells at concentrations of 1000 ng/ml. β-Estradiol enhanced the response of NMS cells at concentrations of 500 ng/ml, had no effect on PHA-stimulated cells, and suppressed the response of ConA-stimulated cells at concentrations greater than 500 ng/ml. Testosterone affected only the ConA response, causing suppression at concentrations above 2000 ng/ml. Corticosterone and progesterone caused 80 and 95% suppression, respectively, of the proliferative response to ConA when compared with non-hormone-treated cells. The possible implications of steroid hormone-induced immunosuppression in the pathogenesis of aspergillosis is discussed. /// Se evaluó la respuesta mitogénica in vitro de linfocitos de sangre entera de pavo a varias concentraciones de hormonas esteroides. La corticosterona a concentraciones entre 1 y 80 ng/ml suprimió significativamente la respuesta proliferativa (incorporación de3H-timidina) a la fitohemaglutinina (FHA) y concanavalina A (ConA). Células no estimuladas con mitógeno fueron suprimidas con concentraciones de corticosterona de más de 5 ng/ml. La progesterona suprimió significativamente a las células no estimuladas con mitógeno a concentraciones de ≥ 80 ng/ml; a las células estimuladas con FHA a concentraciones de ≥ 500 ng/ml y a las células estimuladas con ConA a concentraciones de ≥ 1000 ng/ml. El β-estradiol realzó la respuesta de células no estimuladas a concentraciones de ≥ 500 ng/ml, no tuvo ningún efecto en células estimuladas con FHA y suprimió la respuesta de células estimuladas con ConA a concentraciones de ≥500 ng/ml. La testosterona afectó solamente la respuesta con ConA, causando una supresión a concentraciones de ≥ 2000 ng/ml. La corticosterona y la progesterona causaron una supresión de 80 y 95%, respectivamente, en la respuesta proliferativa a la ConA, en comparación con células no tratadas con hormonas. Se discuten las posibles implicaciones de la inmunosupresión inducida por hormonas esteroides en la patogenesis de la aspergilosis.</abstract><cop>United States</cop><pub>American Association of Avian Pathologists, Inc</pub><pmid>4026731</pmid><doi>10.2307/1590498</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Avian diseases, 1985-04, Vol.29 (2), p.373-383 |
| issn | 0005-2086 1938-4351 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76263357 |
| source | MEDLINE; Jstor Complete Legacy |
| subjects | Animals Aspergillosis ASPERGILLUS Blood Concanavalin A - pharmacology Corticosterone Corticosterone - pharmacology DINDON ENFERMEDADES FUNGOSAS Estradiol - pharmacology HORMONAS ESTEROIDES HORMONE STEROIDIQUE Hormones Hormones - pharmacology In Vitro Techniques LINFOCITOS LYMPHOCYTE Lymphocyte Activation - drug effects LYMPHOCYTES Lymphocytes - drug effects Lymphocytes - immunology Male Mitogens MYCOSE MYCOSES PAVO Phytohemagglutinins - pharmacology Progesterone - pharmacology STEROID HORMONES Steroids Testosterone Testosterone - pharmacology TURKEYS Turkeys - immunology |
| title | The in vitro response of turkey lymphocytes to steroid hormones |
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