AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana
The vacuole is the most prominent organelle of plant cells. Despite its importance for many physiological and developmental aspects of plant life, little is known about its biogenesis and maintenance. Here we show that Arabidopsis plants expressing a dominant-negative version of the AAA (ATPase asso...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2010-10, Vol.64 (1), p.71-85 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Shahriari, Mojgan Keshavaiah, Channa Scheuring, David Sabovljevic, Aneta Pimpl, Peter Häusler, Rainer E Hülskamp, Martin Schellmann, Swen |
| description | The vacuole is the most prominent organelle of plant cells. Despite its importance for many physiological and developmental aspects of plant life, little is known about its biogenesis and maintenance. Here we show that Arabidopsis plants expressing a dominant-negative version of the AAA (ATPase associated with various cellular activities) ATPase AtSKD1 (SUPPRESSOR OF K⁺ TRANSPORT GROWTH DEFECT1) under the control of the trichome-specific GLABRA2 (GL2) promoter exhibit normal vacuolar development in early stages of trichome development. Shortly after its formation, however, the large central vacuole is fragmented and finally disappears completely. Secretion assays with amylase fused to the vacuolar sorting signal of Sporamin show that dominant-negative AtSKD1 inhibits vacuolar trafficking of the reporter that is instead secreted. In addition, trichomes expressing dominant-negative AtSKD1 frequently contain multiple nuclei. Our results suggest that AtSKD1 contributes to vacuolar protein trafficking and thereby to the maintenance of the large central vacuole of plant cells, and might play a role in cell-cycle regulation. |
| doi_str_mv | 10.1111/j.1365-313x.2010.04310.x |
| format | Article |
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Despite its importance for many physiological and developmental aspects of plant life, little is known about its biogenesis and maintenance. Here we show that Arabidopsis plants expressing a dominant-negative version of the AAA (ATPase associated with various cellular activities) ATPase AtSKD1 (SUPPRESSOR OF K⁺ TRANSPORT GROWTH DEFECT1) under the control of the trichome-specific GLABRA2 (GL2) promoter exhibit normal vacuolar development in early stages of trichome development. Shortly after its formation, however, the large central vacuole is fragmented and finally disappears completely. Secretion assays with amylase fused to the vacuolar sorting signal of Sporamin show that dominant-negative AtSKD1 inhibits vacuolar trafficking of the reporter that is instead secreted. In addition, trichomes expressing dominant-negative AtSKD1 frequently contain multiple nuclei. Our results suggest that AtSKD1 contributes to vacuolar protein trafficking and thereby to the maintenance of the large central vacuole of plant cells, and might play a role in cell-cycle regulation.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313x.2010.04310.x</identifier><identifier>PMID: 20663085</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Adenosine triphosphatase ; Adenosine Triphosphatases - genetics ; Adenosine Triphosphatases - metabolism ; Arabidopsis ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Biological and medical sciences ; Cell Nucleus - metabolism ; DNA, Plant - genetics ; endosomal sorting complexes required for transport ; Endosomes - metabolism ; Flowers & plants ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; Mutagenesis, Site-Directed ; MVB ; Plant physiology and development ; Promoter Regions, Genetic ; SKD1 ; vacuole ; vacuoles ; Vacuoles - metabolism ; VPS4</subject><ispartof>The Plant journal : for cell and molecular biology, 2010-10, Vol.64 (1), p.71-85</ispartof><rights>2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2010 The Authors. Journal compilation © 2010 Blackwell Publishing Ltd.</rights><rights>Journal compilation © 2010 Blackwell Publishing Ltd and the Society for Experimental Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5160-63032021587719c79667847c783809e6f18e1cbe7c1663b4eeb9cba4c13e03a13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2010.04310.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2010.04310.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23277550$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20663085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shahriari, Mojgan</creatorcontrib><creatorcontrib>Keshavaiah, Channa</creatorcontrib><creatorcontrib>Scheuring, David</creatorcontrib><creatorcontrib>Sabovljevic, Aneta</creatorcontrib><creatorcontrib>Pimpl, Peter</creatorcontrib><creatorcontrib>Häusler, Rainer E</creatorcontrib><creatorcontrib>Hülskamp, Martin</creatorcontrib><creatorcontrib>Schellmann, Swen</creatorcontrib><title>AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>The vacuole is the most prominent organelle of plant cells. Despite its importance for many physiological and developmental aspects of plant life, little is known about its biogenesis and maintenance. Here we show that Arabidopsis plants expressing a dominant-negative version of the AAA (ATPase associated with various cellular activities) ATPase AtSKD1 (SUPPRESSOR OF K⁺ TRANSPORT GROWTH DEFECT1) under the control of the trichome-specific GLABRA2 (GL2) promoter exhibit normal vacuolar development in early stages of trichome development. Shortly after its formation, however, the large central vacuole is fragmented and finally disappears completely. Secretion assays with amylase fused to the vacuolar sorting signal of Sporamin show that dominant-negative AtSKD1 inhibits vacuolar trafficking of the reporter that is instead secreted. In addition, trichomes expressing dominant-negative AtSKD1 frequently contain multiple nuclei. Our results suggest that AtSKD1 contributes to vacuolar protein trafficking and thereby to the maintenance of the large central vacuole of plant cells, and might play a role in cell-cycle regulation.</description><subject>Adenosine triphosphatase</subject><subject>Adenosine Triphosphatases - genetics</subject><subject>Adenosine Triphosphatases - metabolism</subject><subject>Arabidopsis</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus - metabolism</subject><subject>DNA, Plant - genetics</subject><subject>endosomal sorting complexes required for transport</subject><subject>Endosomes - metabolism</subject><subject>Flowers & plants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>Mutagenesis, Site-Directed</subject><subject>MVB</subject><subject>Plant physiology and development</subject><subject>Promoter Regions, Genetic</subject><subject>SKD1</subject><subject>vacuole</subject><subject>vacuoles</subject><subject>Vacuoles - metabolism</subject><subject>VPS4</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkd1u1DAQhS0EotvCK0CEhHqVxRM7dnLBRVT-qURFtxJ31sR1wKtsvLUT2H17JuwWJCzLY9nfzBzNYSwDvgRar9ZLEKrMBYjdsuD0yqWgc_eALe4_vj1kC14rnmsJxQk7TWnNOWih5GN2UnClBK_KBfvaNE0-7rcua1ZXmCiM15_fQGbDMEbfTqNL2Riyn2in0GPMNuiH0Q04WJeFLmsitv42bJMn7Af2Hgd8wh512Cf39BjP2M27t6uLD_nll_cfL5rL3JZAukiAKHgBZaU11FbXSulKaqsrUfHaqQ4qB7Z12gKJbaVzbW1blBaE4wJBnLHzQ91tDHeTS6PZ-GRd3-PgwpSMVoWsihI4kS_-I9dhigOJM7osJdDWBD07QlO7cbdmG_0G497cz4qAl0cAk8W-izQEn_5xotBUbe72-sD98r3b__0HbmbvzNrMFpnZIjN7Z_54Z3ZmdfVpvlH-80N-h8Hg90g9bq6JFByqWnIN4jcSk5KW</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Shahriari, Mojgan</creator><creator>Keshavaiah, Channa</creator><creator>Scheuring, David</creator><creator>Sabovljevic, Aneta</creator><creator>Pimpl, Peter</creator><creator>Häusler, Rainer E</creator><creator>Hülskamp, Martin</creator><creator>Schellmann, Swen</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201010</creationdate><title>AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana</title><author>Shahriari, Mojgan ; Keshavaiah, Channa ; Scheuring, David ; Sabovljevic, Aneta ; Pimpl, Peter ; Häusler, Rainer E ; Hülskamp, Martin ; Schellmann, Swen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5160-63032021587719c79667847c783809e6f18e1cbe7c1663b4eeb9cba4c13e03a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adenosine triphosphatase</topic><topic>Adenosine Triphosphatases - genetics</topic><topic>Adenosine Triphosphatases - metabolism</topic><topic>Arabidopsis</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleus - metabolism</topic><topic>DNA, Plant - genetics</topic><topic>endosomal sorting complexes required for transport</topic><topic>Endosomes - metabolism</topic><topic>Flowers & plants</topic><topic>Fundamental and applied biological sciences. 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| ispartof | The Plant journal : for cell and molecular biology, 2010-10, Vol.64 (1), p.71-85 |
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| subjects | Adenosine triphosphatase Adenosine Triphosphatases - genetics Adenosine Triphosphatases - metabolism Arabidopsis Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Biological and medical sciences Cell Nucleus - metabolism DNA, Plant - genetics endosomal sorting complexes required for transport Endosomes - metabolism Flowers & plants Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Mutagenesis, Site-Directed MVB Plant physiology and development Promoter Regions, Genetic SKD1 vacuole vacuoles Vacuoles - metabolism VPS4 |
| title | AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana |
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