Involvement of Cl − transport in forskolin-induced elevation of endocochlear potential
To determine the possible involvement of Cl − transport in the forskolin-induced endocochlear potential (EP) elevation, the effect of forskolin on the EP was examined in Cl −-free artificial perilymph (aPL) as well as in the presence of Cl − channel blockers. The perfusion of scala vestibuli (SV) wi...
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| Veröffentlicht in: | Hearing research 1993-12, Vol.71 (1), p.23-27 |
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| creator | Kitano, Ikuo Doi, Katsumi Mori, Nozomu Matsunaga, Takashi |
| description | To determine the possible involvement of Cl
− transport in the forskolin-induced endocochlear potential (EP) elevation, the effect of forskolin on the EP was examined in Cl
−-free artificial perilymph (aPL) as well as in the presence of Cl
− channel blockers. The perfusion of scala vestibuli (SV) with forskolin (200 μM) dissolved in Cl
−-free aPL failed to produce an EP elevation, while SV perfusion of forskolin dissolved in normal aPL elevated the EP. The application of DPC and IAA-94 (blockers of cAMP-activated Cl
− channel) into SV completely suppressed the forskolin-induced EP elevation, while niflumic acid (a Ca
2+-activated Cl
− channel blocker) failed to do so. IAA-94 applied into scala tympani (ST) did not suppress this EP elevation. The results suggest that adenylate cyclase may modulate the EP by changing Cl
− transport between SV and scala media (SM) across Reissner's membrane. |
| doi_str_mv | 10.1016/0378-5955(93)90017-U |
| format | Article |
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− transport in the forskolin-induced endocochlear potential (EP) elevation, the effect of forskolin on the EP was examined in Cl
−-free artificial perilymph (aPL) as well as in the presence of Cl
− channel blockers. The perfusion of scala vestibuli (SV) with forskolin (200 μM) dissolved in Cl
−-free aPL failed to produce an EP elevation, while SV perfusion of forskolin dissolved in normal aPL elevated the EP. The application of DPC and IAA-94 (blockers of cAMP-activated Cl
− channel) into SV completely suppressed the forskolin-induced EP elevation, while niflumic acid (a Ca
2+-activated Cl
− channel blocker) failed to do so. IAA-94 applied into scala tympani (ST) did not suppress this EP elevation. The results suggest that adenylate cyclase may modulate the EP by changing Cl
− transport between SV and scala media (SM) across Reissner's membrane.</description><identifier>ISSN: 0378-5955</identifier><identifier>EISSN: 1878-5891</identifier><identifier>DOI: 10.1016/0378-5955(93)90017-U</identifier><identifier>PMID: 8113141</identifier><identifier>CODEN: HERED3</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adenylate cyclase ; Adenylyl Cyclases - metabolism ; Animals ; Biological and medical sciences ; Biological Transport ; cAMP-activated Cl − channel ; Chloride Channels - drug effects ; Chlorides - metabolism ; Cl − transport ; Cochlea - drug effects ; Cochlea - metabolism ; Cochlear Duct - drug effects ; Cochlear Duct - metabolism ; Colforsin - pharmacology ; Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation ; Endocochlear potential ; Evoked Potentials, Auditory - drug effects ; Forskolin ; Fundamental and applied biological sciences. Psychology ; Glycolates - pharmacology ; Guinea Pigs ; ortho-Aminobenzoates - pharmacology ; Perilymph - drug effects ; Perilymph - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Hearing research, 1993-12, Vol.71 (1), p.23-27</ispartof><rights>1993</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-9c28d04580d38e3ecfa8010cbcd5b57aef93ea4bee8fe2abc88bcd89000e95513</citedby><cites>FETCH-LOGICAL-c386t-9c28d04580d38e3ecfa8010cbcd5b57aef93ea4bee8fe2abc88bcd89000e95513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-5955(93)90017-U$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3928975$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8113141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kitano, Ikuo</creatorcontrib><creatorcontrib>Doi, Katsumi</creatorcontrib><creatorcontrib>Mori, Nozomu</creatorcontrib><creatorcontrib>Matsunaga, Takashi</creatorcontrib><title>Involvement of Cl − transport in forskolin-induced elevation of endocochlear potential</title><title>Hearing research</title><addtitle>Hear Res</addtitle><description>To determine the possible involvement of Cl
− transport in the forskolin-induced endocochlear potential (EP) elevation, the effect of forskolin on the EP was examined in Cl
−-free artificial perilymph (aPL) as well as in the presence of Cl
− channel blockers. The perfusion of scala vestibuli (SV) with forskolin (200 μM) dissolved in Cl
−-free aPL failed to produce an EP elevation, while SV perfusion of forskolin dissolved in normal aPL elevated the EP. The application of DPC and IAA-94 (blockers of cAMP-activated Cl
− channel) into SV completely suppressed the forskolin-induced EP elevation, while niflumic acid (a Ca
2+-activated Cl
− channel blocker) failed to do so. IAA-94 applied into scala tympani (ST) did not suppress this EP elevation. The results suggest that adenylate cyclase may modulate the EP by changing Cl
− transport between SV and scala media (SM) across Reissner's membrane.</description><subject>Adenylate cyclase</subject><subject>Adenylyl Cyclases - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>cAMP-activated Cl − channel</subject><subject>Chloride Channels - drug effects</subject><subject>Chlorides - metabolism</subject><subject>Cl − transport</subject><subject>Cochlea - drug effects</subject><subject>Cochlea - metabolism</subject><subject>Cochlear Duct - drug effects</subject><subject>Cochlear Duct - metabolism</subject><subject>Colforsin - pharmacology</subject><subject>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</subject><subject>Endocochlear potential</subject><subject>Evoked Potentials, Auditory - drug effects</subject><subject>Forskolin</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycolates - pharmacology</subject><subject>Guinea Pigs</subject><subject>ortho-Aminobenzoates - pharmacology</subject><subject>Perilymph - drug effects</subject><subject>Perilymph - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0378-5955</issn><issn>1878-5891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1KxDAURoMoOo6-gUIXIrqoJk07TTaCDP4MDLhxwF1I01uMpsmYdAZ8A9c-ok9i6pRZukkg3_kuuQehE4KvCCaTa0xLlha8KC44veQYkzJd7KARYf0z42QXjbbIAToM4S0yBc2zfbTPCKEkJyP0MrNrZ9bQgu0S1yRTk_x8fSedlzYsne8SbZPG-fDujLaptvVKQZ2AgbXstLN9BWztlFOvBqRPlq6Lk7Q0R2ivkSbA8XCP0eL-7nn6mM6fHmbT23mqKJt0KVcZq3FeMFxTBhRUIxkmWFWqLqqilNBwCjKvAFgDmawUYzFicV0McS9Cx-h8M3fp3ccKQidaHRQYIy24VRDlJKMTHo8xyjeg8i4ED41Yet1K_ykIFr1Q0dsSvS3BqfgTKhaxdjrMX1Ut1NvSYDDmZ0Mug5KmieKUDluM8ozxsojYzQaD6GKtwYugNNgoU3tQnaid_v8fvzB9lKg</recordid><startdate>19931201</startdate><enddate>19931201</enddate><creator>Kitano, Ikuo</creator><creator>Doi, Katsumi</creator><creator>Mori, Nozomu</creator><creator>Matsunaga, Takashi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>19931201</creationdate><title>Involvement of Cl − transport in forskolin-induced elevation of endocochlear potential</title><author>Kitano, Ikuo ; Doi, Katsumi ; Mori, Nozomu ; Matsunaga, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-9c28d04580d38e3ecfa8010cbcd5b57aef93ea4bee8fe2abc88bcd89000e95513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenylate cyclase</topic><topic>Adenylyl Cyclases - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>cAMP-activated Cl − channel</topic><topic>Chloride Channels - drug effects</topic><topic>Chlorides - metabolism</topic><topic>Cl − transport</topic><topic>Cochlea - drug effects</topic><topic>Cochlea - metabolism</topic><topic>Cochlear Duct - drug effects</topic><topic>Cochlear Duct - metabolism</topic><topic>Colforsin - pharmacology</topic><topic>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</topic><topic>Endocochlear potential</topic><topic>Evoked Potentials, Auditory - drug effects</topic><topic>Forskolin</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycolates - pharmacology</topic><topic>Guinea Pigs</topic><topic>ortho-Aminobenzoates - pharmacology</topic><topic>Perilymph - drug effects</topic><topic>Perilymph - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kitano, Ikuo</creatorcontrib><creatorcontrib>Doi, Katsumi</creatorcontrib><creatorcontrib>Mori, Nozomu</creatorcontrib><creatorcontrib>Matsunaga, Takashi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Hearing research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kitano, Ikuo</au><au>Doi, Katsumi</au><au>Mori, Nozomu</au><au>Matsunaga, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of Cl − transport in forskolin-induced elevation of endocochlear potential</atitle><jtitle>Hearing research</jtitle><addtitle>Hear Res</addtitle><date>1993-12-01</date><risdate>1993</risdate><volume>71</volume><issue>1</issue><spage>23</spage><epage>27</epage><pages>23-27</pages><issn>0378-5955</issn><eissn>1878-5891</eissn><coden>HERED3</coden><abstract>To determine the possible involvement of Cl
− transport in the forskolin-induced endocochlear potential (EP) elevation, the effect of forskolin on the EP was examined in Cl
−-free artificial perilymph (aPL) as well as in the presence of Cl
− channel blockers. The perfusion of scala vestibuli (SV) with forskolin (200 μM) dissolved in Cl
−-free aPL failed to produce an EP elevation, while SV perfusion of forskolin dissolved in normal aPL elevated the EP. The application of DPC and IAA-94 (blockers of cAMP-activated Cl
− channel) into SV completely suppressed the forskolin-induced EP elevation, while niflumic acid (a Ca
2+-activated Cl
− channel blocker) failed to do so. IAA-94 applied into scala tympani (ST) did not suppress this EP elevation. The results suggest that adenylate cyclase may modulate the EP by changing Cl
− transport between SV and scala media (SM) across Reissner's membrane.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8113141</pmid><doi>10.1016/0378-5955(93)90017-U</doi><tpages>5</tpages></addata></record> |
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| subjects | Adenylate cyclase Adenylyl Cyclases - metabolism Animals Biological and medical sciences Biological Transport cAMP-activated Cl − channel Chloride Channels - drug effects Chlorides - metabolism Cl − transport Cochlea - drug effects Cochlea - metabolism Cochlear Duct - drug effects Cochlear Duct - metabolism Colforsin - pharmacology Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation Endocochlear potential Evoked Potentials, Auditory - drug effects Forskolin Fundamental and applied biological sciences. Psychology Glycolates - pharmacology Guinea Pigs ortho-Aminobenzoates - pharmacology Perilymph - drug effects Perilymph - metabolism Vertebrates: nervous system and sense organs |
| title | Involvement of Cl − transport in forskolin-induced elevation of endocochlear potential |
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