Identification of the fish species of raw or cold-smoked salmon and salmon caviar by single-strand conformation polymorphism (SSCP) analysis

Differentiation between ten salmonid fish species belonging to the genera Salmo, Oncorhynchus and Salvelinus was achieved by single strand conformation polymorphism analysis (SSCP) of PCR products of mitochondrial and nuclear genes. Amplicons (300-460 bp in size) of the genes for cytochrome b, parva...

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Veröffentlicht in:European food research & technology 2005-05, Vol.220 (5-6), p.625-632
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description Differentiation between ten salmonid fish species belonging to the genera Salmo, Oncorhynchus and Salvelinus was achieved by single strand conformation polymorphism analysis (SSCP) of PCR products of mitochondrial and nuclear genes. Amplicons (300-460 bp in size) of the genes for cytochrome b, parvalbumin and growth hormone gave species-specific patterns of single-stranded DNA in native polyacrylamide gel electrophoresis (PAGE). The method was successfully used to identify products from raw or cold-smoked salmon, as well as from salmon roe.[PUBLICATION ABSTRACT]
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Psychology ; genes ; mitochondrial DNA ; molecular sequence data ; nucleotide sequences ; Oncorhynchus ; parvalbumin ; polymerase chain reaction ; Polymorphism ; Salmo ; Salmon ; Salmonidae ; Salvelinus ; single-stranded conformational polymorphism ; single-stranded DNA ; smoked fish ; somatotropin ; species differences</subject><ispartof>European food research &amp; technology, 2005-05, Vol.220 (5-6), p.625-632</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-be149e72d2f3ba77534fb78cde3af7f8a1e37e1333b9431a61f0d071dc627f893</citedby><cites>FETCH-LOGICAL-c358t-be149e72d2f3ba77534fb78cde3af7f8a1e37e1333b9431a61f0d071dc627f893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=16824251$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Rehbein, H</creatorcontrib><title>Identification of the fish species of raw or cold-smoked salmon and salmon caviar by single-strand conformation polymorphism (SSCP) analysis</title><title>European food research &amp; technology</title><description>Differentiation between ten salmonid fish species belonging to the genera Salmo, Oncorhynchus and Salvelinus was achieved by single strand conformation polymorphism analysis (SSCP) of PCR products of mitochondrial and nuclear genes. 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source Springer Nature - Complete Springer Journals
subjects animal proteins
Biological and medical sciences
Curing
cytochrome b
DNA
Fish
Fish and seafood industries
fish products
fish roe
Food industries
Fundamental and applied biological sciences. Psychology
genes
mitochondrial DNA
molecular sequence data
nucleotide sequences
Oncorhynchus
parvalbumin
polymerase chain reaction
Polymorphism
Salmo
Salmon
Salmonidae
Salvelinus
single-stranded conformational polymorphism
single-stranded DNA
smoked fish
somatotropin
species differences
title Identification of the fish species of raw or cold-smoked salmon and salmon caviar by single-strand conformation polymorphism (SSCP) analysis
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