Identification of the fish species of raw or cold-smoked salmon and salmon caviar by single-strand conformation polymorphism (SSCP) analysis
Differentiation between ten salmonid fish species belonging to the genera Salmo, Oncorhynchus and Salvelinus was achieved by single strand conformation polymorphism analysis (SSCP) of PCR products of mitochondrial and nuclear genes. Amplicons (300-460 bp in size) of the genes for cytochrome b, parva...
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Veröffentlicht in: | European food research & technology 2005-05, Vol.220 (5-6), p.625-632 |
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description | Differentiation between ten salmonid fish species belonging to the genera Salmo, Oncorhynchus and Salvelinus was achieved by single strand conformation polymorphism analysis (SSCP) of PCR products of mitochondrial and nuclear genes. Amplicons (300-460 bp in size) of the genes for cytochrome b, parvalbumin and growth hormone gave species-specific patterns of single-stranded DNA in native polyacrylamide gel electrophoresis (PAGE). The method was successfully used to identify products from raw or cold-smoked salmon, as well as from salmon roe.[PUBLICATION ABSTRACT] |
doi_str_mv | 10.1007/s00217-004-1067-2 |
format | Article |
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Amplicons (300-460 bp in size) of the genes for cytochrome b, parvalbumin and growth hormone gave species-specific patterns of single-stranded DNA in native polyacrylamide gel electrophoresis (PAGE). The method was successfully used to identify products from raw or cold-smoked salmon, as well as from salmon roe.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 1438-2377</identifier><identifier>EISSN: 1438-2385</identifier><identifier>DOI: 10.1007/s00217-004-1067-2</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>animal proteins ; Biological and medical sciences ; Curing ; cytochrome b ; DNA ; Fish ; Fish and seafood industries ; fish products ; fish roe ; Food industries ; Fundamental and applied biological sciences. Psychology ; genes ; mitochondrial DNA ; molecular sequence data ; nucleotide sequences ; Oncorhynchus ; parvalbumin ; polymerase chain reaction ; Polymorphism ; Salmo ; Salmon ; Salmonidae ; Salvelinus ; single-stranded conformational polymorphism ; single-stranded DNA ; smoked fish ; somatotropin ; species differences</subject><ispartof>European food research & technology, 2005-05, Vol.220 (5-6), p.625-632</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-be149e72d2f3ba77534fb78cde3af7f8a1e37e1333b9431a61f0d071dc627f893</citedby><cites>FETCH-LOGICAL-c358t-be149e72d2f3ba77534fb78cde3af7f8a1e37e1333b9431a61f0d071dc627f893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16824251$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Rehbein, H</creatorcontrib><title>Identification of the fish species of raw or cold-smoked salmon and salmon caviar by single-strand conformation polymorphism (SSCP) analysis</title><title>European food research & technology</title><description>Differentiation between ten salmonid fish species belonging to the genera Salmo, Oncorhynchus and Salvelinus was achieved by single strand conformation polymorphism analysis (SSCP) of PCR products of mitochondrial and nuclear genes. Amplicons (300-460 bp in size) of the genes for cytochrome b, parvalbumin and growth hormone gave species-specific patterns of single-stranded DNA in native polyacrylamide gel electrophoresis (PAGE). The method was successfully used to identify products from raw or cold-smoked salmon, as well as from salmon roe.[PUBLICATION ABSTRACT]</description><subject>animal proteins</subject><subject>Biological and medical sciences</subject><subject>Curing</subject><subject>cytochrome b</subject><subject>DNA</subject><subject>Fish</subject><subject>Fish and seafood industries</subject><subject>fish products</subject><subject>fish roe</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>mitochondrial DNA</subject><subject>molecular sequence data</subject><subject>nucleotide sequences</subject><subject>Oncorhynchus</subject><subject>parvalbumin</subject><subject>polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Salmo</subject><subject>Salmon</subject><subject>Salmonidae</subject><subject>Salvelinus</subject><subject>single-stranded conformational polymorphism</subject><subject>single-stranded DNA</subject><subject>smoked fish</subject><subject>somatotropin</subject><subject>species differences</subject><issn>1438-2377</issn><issn>1438-2385</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkd9qFTEQxhdRsFYfwCuDINaLaP7sJnsu5aC1UGjh2Oswm016UrObNbPHct7BhzbLlha8mmHm930T8lXVW84-c8b0F2RMcE0ZqylnSlPxrDrhtWypkG3z_LHX-mX1CvGOsWajeH1S_b3o3TgHHyzMIY0keTLvHfEB9wQnZ4PDZZbhnqRMbIo9xSH9cj1BiEMRwPjYWvgTIJPuSDCMt9FRnPOytmn0KQ_rgSnF45DytA84kLPdbnv9qXhAPGLA19ULDxHdm4d6Wt18__Zz-4NeXp1fbL9eUiubdqad4_XGadELLzvQupG173RreyfBa98Cd1I7LqXsNrXkoLhnPdO8t0qU9UaeVh9X3ymn3weHsxkCWhcjjC4d0GglRMtFwwr5_j_yLh1yeS4aJQUTTSNEgfgK2ZwQs_NmymGAfDScmSUds6ZjSjpmSccsmg8PxoAWoi8fZQM-CVUratHwwr1bOQ_JwG0uzM1OMC5ZMaqV0vIfn9eZ-A</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Rehbein, H</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QR</scope><scope>7RQ</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X2</scope><scope>7XB</scope><scope>87Z</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>F~G</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>L.-</scope><scope>L6V</scope><scope>M0C</scope><scope>M0K</scope><scope>M2P</scope><scope>M7S</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7TM</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20050501</creationdate><title>Identification of the fish species of raw or cold-smoked salmon and salmon caviar by single-strand conformation polymorphism (SSCP) analysis</title><author>Rehbein, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-be149e72d2f3ba77534fb78cde3af7f8a1e37e1333b9431a61f0d071dc627f893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>animal proteins</topic><topic>Biological and medical sciences</topic><topic>Curing</topic><topic>cytochrome b</topic><topic>DNA</topic><topic>Fish</topic><topic>Fish and seafood industries</topic><topic>fish products</topic><topic>fish roe</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>mitochondrial DNA</topic><topic>molecular sequence data</topic><topic>nucleotide sequences</topic><topic>Oncorhynchus</topic><topic>parvalbumin</topic><topic>polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Salmo</topic><topic>Salmon</topic><topic>Salmonidae</topic><topic>Salvelinus</topic><topic>single-stranded conformational polymorphism</topic><topic>single-stranded DNA</topic><topic>smoked fish</topic><topic>somatotropin</topic><topic>species differences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rehbein, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Career & Technical Education Database</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Engineering Collection</collection><collection>ABI/INFORM Global</collection><collection>Agricultural Science Database</collection><collection>Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Nucleic Acids Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>European food research & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rehbein, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of the fish species of raw or cold-smoked salmon and salmon caviar by single-strand conformation polymorphism (SSCP) analysis</atitle><jtitle>European food research & technology</jtitle><date>2005-05-01</date><risdate>2005</risdate><volume>220</volume><issue>5-6</issue><spage>625</spage><epage>632</epage><pages>625-632</pages><issn>1438-2377</issn><eissn>1438-2385</eissn><abstract>Differentiation between ten salmonid fish species belonging to the genera Salmo, Oncorhynchus and Salvelinus was achieved by single strand conformation polymorphism analysis (SSCP) of PCR products of mitochondrial and nuclear genes. Amplicons (300-460 bp in size) of the genes for cytochrome b, parvalbumin and growth hormone gave species-specific patterns of single-stranded DNA in native polyacrylamide gel electrophoresis (PAGE). The method was successfully used to identify products from raw or cold-smoked salmon, as well as from salmon roe.[PUBLICATION ABSTRACT]</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s00217-004-1067-2</doi><tpages>8</tpages></addata></record> |
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subjects | animal proteins Biological and medical sciences Curing cytochrome b DNA Fish Fish and seafood industries fish products fish roe Food industries Fundamental and applied biological sciences. Psychology genes mitochondrial DNA molecular sequence data nucleotide sequences Oncorhynchus parvalbumin polymerase chain reaction Polymorphism Salmo Salmon Salmonidae Salvelinus single-stranded conformational polymorphism single-stranded DNA smoked fish somatotropin species differences |
title | Identification of the fish species of raw or cold-smoked salmon and salmon caviar by single-strand conformation polymorphism (SSCP) analysis |
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