Particle and naked RNA mycoviruses in industrially cultivated mushroom Pleurotus ostreatus in China
Many cultivated mushroom strains, such as Pleurotus ostreatus TD300, displayed symptoms of degeneration. A spherical virus POSV and four dsRNA segments were extracted from mycelium of P. ostreatus TD300. POSV had a diameter of 23 nm and encapsidated a 2.5 kb dsRNA segment with coat proteins whose mo...
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| Veröffentlicht in: | Fungal biology 2010-05, Vol.114 (5), p.507-513 |
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| creator | Qiu, Liyou Li, Yanpeng Liu, Yingmiao Gao, Yuqian Qi, Yuancheng Shen, Jinwen |
| description | Many cultivated mushroom strains, such as
Pleurotus ostreatus TD300, displayed symptoms of degeneration. A spherical virus POSV and four dsRNA segments were extracted from mycelium of
P. ostreatus TD300. POSV had a diameter of 23
nm and encapsidated a 2.5
kb dsRNA segment with coat proteins whose molecular weights were 39
kDa and 30
kDa. Four dsRNA segments were 8.2
kb, 2.5
kb, 2.0
kb, and 1.1
kb in size, respectively. The 1.1
kb dsRNA segment often escaped detection. The cDNA and the amino acid sequences of the 8.2
kb dsRNA were homologous to those of RNA-dependent RNA polymerases (RDRP) of ssRNA oyster mushroom spherical virus (OMSV), and contained conserved motifs A to D which were almost identical to those in RDRP of OMSV. The cDNA and amino acid sequences of the 2.5
kb and 2.0
kb dsRNA segments were homologous to that of RDRP and capsid protein of dsRNA virus
P. ostreatus virus 1 (PoV1), respectively. In particular, the amino acid sequence of 2.5
kb dsRNA segment had high identity with the conserved motifs A to C in RDRP of PoV1, a
Partiviridae virus. After eliminating the viruses in
P. ostreatus TD300, the symptoms of degeneration completely disappeared. The results reveal that
P. ostreatus TD300 was at least infected by a particle virus POSV, and two naked viruses, one was a dsRNA virus with a 2.0
kb dsRNA segment, the other was an ssRNA virus whose replicating form of genome was an 8.2
kb dsRNA segment. Mycoviruses infection is a causative agent of mushroom strain degeneration. |
| doi_str_mv | 10.1016/j.funbio.2010.04.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_762278557</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1878614610000619</els_id><sourcerecordid>758832887</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-2dcc6a59cb35088122d69d1797cf7c23bf92e5d2191205497e4d363af7c3f1b23</originalsourceid><addsrcrecordid>eNqFkVtLAzEQhYMoKtV_ILJvPrXmsrtJXgQp3kC0iD6HbDJLU3c3mmwK_femVH3UITCH5DsZmIPQGcEzgkl9uZq1aWicn1Gcr3A5w5jsoWMiuJjWpKb7v7qsj9BpjCucixEmJD9ERxTLkmXsGJmFDqMzHRR6sMWg38EWL0_XRb8xfu1CihALN-RjUxyD0123KUzqRrfWY0b7FJfB-75YdJCCH1MsfOZAb1X2zZdu0CfooNVdhNPvPkFvtzev8_vp4_Pdw_z6cWrKSo5Tao2pdSVNwyosBKHU1tISLrlpuaGsaSWFylIiCcVVKTmUltVM50fWkoayCbrY_fsR_GeCOKreRQNdpwfwKSpeU8pFVfH_yUoIRoXYkuWONMHHGKBVH8H1OmwUwWobhVqpXRRqG4XCpcpRZNv594DU9GB_TT-Lz8DVDoC8kLWDoKJxMBiwLoAZlfXu7wlfWrCcpw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>758832887</pqid></control><display><type>article</type><title>Particle and naked RNA mycoviruses in industrially cultivated mushroom Pleurotus ostreatus in China</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Qiu, Liyou ; Li, Yanpeng ; Liu, Yingmiao ; Gao, Yuqian ; Qi, Yuancheng ; Shen, Jinwen</creator><creatorcontrib>Qiu, Liyou ; Li, Yanpeng ; Liu, Yingmiao ; Gao, Yuqian ; Qi, Yuancheng ; Shen, Jinwen</creatorcontrib><description>Many cultivated mushroom strains, such as
Pleurotus ostreatus TD300, displayed symptoms of degeneration. A spherical virus POSV and four dsRNA segments were extracted from mycelium of
P. ostreatus TD300. POSV had a diameter of 23
nm and encapsidated a 2.5
kb dsRNA segment with coat proteins whose molecular weights were 39
kDa and 30
kDa. Four dsRNA segments were 8.2
kb, 2.5
kb, 2.0
kb, and 1.1
kb in size, respectively. The 1.1
kb dsRNA segment often escaped detection. The cDNA and the amino acid sequences of the 8.2
kb dsRNA were homologous to those of RNA-dependent RNA polymerases (RDRP) of ssRNA oyster mushroom spherical virus (OMSV), and contained conserved motifs A to D which were almost identical to those in RDRP of OMSV. The cDNA and amino acid sequences of the 2.5
kb and 2.0
kb dsRNA segments were homologous to that of RDRP and capsid protein of dsRNA virus
P. ostreatus virus 1 (PoV1), respectively. In particular, the amino acid sequence of 2.5
kb dsRNA segment had high identity with the conserved motifs A to C in RDRP of PoV1, a
Partiviridae virus. After eliminating the viruses in
P. ostreatus TD300, the symptoms of degeneration completely disappeared. The results reveal that
P. ostreatus TD300 was at least infected by a particle virus POSV, and two naked viruses, one was a dsRNA virus with a 2.0
kb dsRNA segment, the other was an ssRNA virus whose replicating form of genome was an 8.2
kb dsRNA segment. Mycoviruses infection is a causative agent of mushroom strain degeneration.</description><identifier>ISSN: 1878-6146</identifier><identifier>EISSN: 1878-6162</identifier><identifier>DOI: 10.1016/j.funbio.2010.04.001</identifier><identifier>PMID: 20943162</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Amino acid sequence ; Base Sequence ; Industrial Microbiology ; Molecular Sequence Data ; Naked virus ; Particle virus ; Pleurotus - growth & development ; Pleurotus - virology ; Pleurotus ostreatus ; RNA mycovirus ; RNA Viruses - genetics ; RNA Viruses - isolation & purification ; RNA Viruses - physiology ; RNA Viruses - ultrastructure ; RNA, Viral - genetics ; Sequence Alignment ; Strain degeneration ; Viral Proteins - genetics ; Virion - genetics ; Virion - isolation & purification ; Virion - physiology ; Virion - ultrastructure</subject><ispartof>Fungal biology, 2010-05, Vol.114 (5), p.507-513</ispartof><rights>2010 The British Mycological Society</rights><rights>Copyright © 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-2dcc6a59cb35088122d69d1797cf7c23bf92e5d2191205497e4d363af7c3f1b23</citedby><cites>FETCH-LOGICAL-c459t-2dcc6a59cb35088122d69d1797cf7c23bf92e5d2191205497e4d363af7c3f1b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.funbio.2010.04.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20943162$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiu, Liyou</creatorcontrib><creatorcontrib>Li, Yanpeng</creatorcontrib><creatorcontrib>Liu, Yingmiao</creatorcontrib><creatorcontrib>Gao, Yuqian</creatorcontrib><creatorcontrib>Qi, Yuancheng</creatorcontrib><creatorcontrib>Shen, Jinwen</creatorcontrib><title>Particle and naked RNA mycoviruses in industrially cultivated mushroom Pleurotus ostreatus in China</title><title>Fungal biology</title><addtitle>Fungal Biol</addtitle><description>Many cultivated mushroom strains, such as
Pleurotus ostreatus TD300, displayed symptoms of degeneration. A spherical virus POSV and four dsRNA segments were extracted from mycelium of
P. ostreatus TD300. POSV had a diameter of 23
nm and encapsidated a 2.5
kb dsRNA segment with coat proteins whose molecular weights were 39
kDa and 30
kDa. Four dsRNA segments were 8.2
kb, 2.5
kb, 2.0
kb, and 1.1
kb in size, respectively. The 1.1
kb dsRNA segment often escaped detection. The cDNA and the amino acid sequences of the 8.2
kb dsRNA were homologous to those of RNA-dependent RNA polymerases (RDRP) of ssRNA oyster mushroom spherical virus (OMSV), and contained conserved motifs A to D which were almost identical to those in RDRP of OMSV. The cDNA and amino acid sequences of the 2.5
kb and 2.0
kb dsRNA segments were homologous to that of RDRP and capsid protein of dsRNA virus
P. ostreatus virus 1 (PoV1), respectively. In particular, the amino acid sequence of 2.5
kb dsRNA segment had high identity with the conserved motifs A to C in RDRP of PoV1, a
Partiviridae virus. After eliminating the viruses in
P. ostreatus TD300, the symptoms of degeneration completely disappeared. The results reveal that
P. ostreatus TD300 was at least infected by a particle virus POSV, and two naked viruses, one was a dsRNA virus with a 2.0
kb dsRNA segment, the other was an ssRNA virus whose replicating form of genome was an 8.2
kb dsRNA segment. Mycoviruses infection is a causative agent of mushroom strain degeneration.</description><subject>Amino acid sequence</subject><subject>Base Sequence</subject><subject>Industrial Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Naked virus</subject><subject>Particle virus</subject><subject>Pleurotus - growth & development</subject><subject>Pleurotus - virology</subject><subject>Pleurotus ostreatus</subject><subject>RNA mycovirus</subject><subject>RNA Viruses - genetics</subject><subject>RNA Viruses - isolation & purification</subject><subject>RNA Viruses - physiology</subject><subject>RNA Viruses - ultrastructure</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Alignment</subject><subject>Strain degeneration</subject><subject>Viral Proteins - genetics</subject><subject>Virion - genetics</subject><subject>Virion - isolation & purification</subject><subject>Virion - physiology</subject><subject>Virion - ultrastructure</subject><issn>1878-6146</issn><issn>1878-6162</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtLAzEQhYMoKtV_ILJvPrXmsrtJXgQp3kC0iD6HbDJLU3c3mmwK_femVH3UITCH5DsZmIPQGcEzgkl9uZq1aWicn1Gcr3A5w5jsoWMiuJjWpKb7v7qsj9BpjCucixEmJD9ERxTLkmXsGJmFDqMzHRR6sMWg38EWL0_XRb8xfu1CihALN-RjUxyD0123KUzqRrfWY0b7FJfB-75YdJCCH1MsfOZAb1X2zZdu0CfooNVdhNPvPkFvtzev8_vp4_Pdw_z6cWrKSo5Tao2pdSVNwyosBKHU1tISLrlpuaGsaSWFylIiCcVVKTmUltVM50fWkoayCbrY_fsR_GeCOKreRQNdpwfwKSpeU8pFVfH_yUoIRoXYkuWONMHHGKBVH8H1OmwUwWobhVqpXRRqG4XCpcpRZNv594DU9GB_TT-Lz8DVDoC8kLWDoKJxMBiwLoAZlfXu7wlfWrCcpw</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Qiu, Liyou</creator><creator>Li, Yanpeng</creator><creator>Liu, Yingmiao</creator><creator>Gao, Yuqian</creator><creator>Qi, Yuancheng</creator><creator>Shen, Jinwen</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope></search><sort><creationdate>20100501</creationdate><title>Particle and naked RNA mycoviruses in industrially cultivated mushroom Pleurotus ostreatus in China</title><author>Qiu, Liyou ; Li, Yanpeng ; Liu, Yingmiao ; Gao, Yuqian ; Qi, Yuancheng ; Shen, Jinwen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-2dcc6a59cb35088122d69d1797cf7c23bf92e5d2191205497e4d363af7c3f1b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Amino acid sequence</topic><topic>Base Sequence</topic><topic>Industrial Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Naked virus</topic><topic>Particle virus</topic><topic>Pleurotus - growth & development</topic><topic>Pleurotus - virology</topic><topic>Pleurotus ostreatus</topic><topic>RNA mycovirus</topic><topic>RNA Viruses - genetics</topic><topic>RNA Viruses - isolation & purification</topic><topic>RNA Viruses - physiology</topic><topic>RNA Viruses - ultrastructure</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Alignment</topic><topic>Strain degeneration</topic><topic>Viral Proteins - genetics</topic><topic>Virion - genetics</topic><topic>Virion - isolation & purification</topic><topic>Virion - physiology</topic><topic>Virion - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiu, Liyou</creatorcontrib><creatorcontrib>Li, Yanpeng</creatorcontrib><creatorcontrib>Liu, Yingmiao</creatorcontrib><creatorcontrib>Gao, Yuqian</creatorcontrib><creatorcontrib>Qi, Yuancheng</creatorcontrib><creatorcontrib>Shen, Jinwen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Fungal biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiu, Liyou</au><au>Li, Yanpeng</au><au>Liu, Yingmiao</au><au>Gao, Yuqian</au><au>Qi, Yuancheng</au><au>Shen, Jinwen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Particle and naked RNA mycoviruses in industrially cultivated mushroom Pleurotus ostreatus in China</atitle><jtitle>Fungal biology</jtitle><addtitle>Fungal Biol</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>114</volume><issue>5</issue><spage>507</spage><epage>513</epage><pages>507-513</pages><issn>1878-6146</issn><eissn>1878-6162</eissn><abstract>Many cultivated mushroom strains, such as
Pleurotus ostreatus TD300, displayed symptoms of degeneration. A spherical virus POSV and four dsRNA segments were extracted from mycelium of
P. ostreatus TD300. POSV had a diameter of 23
nm and encapsidated a 2.5
kb dsRNA segment with coat proteins whose molecular weights were 39
kDa and 30
kDa. Four dsRNA segments were 8.2
kb, 2.5
kb, 2.0
kb, and 1.1
kb in size, respectively. The 1.1
kb dsRNA segment often escaped detection. The cDNA and the amino acid sequences of the 8.2
kb dsRNA were homologous to those of RNA-dependent RNA polymerases (RDRP) of ssRNA oyster mushroom spherical virus (OMSV), and contained conserved motifs A to D which were almost identical to those in RDRP of OMSV. The cDNA and amino acid sequences of the 2.5
kb and 2.0
kb dsRNA segments were homologous to that of RDRP and capsid protein of dsRNA virus
P. ostreatus virus 1 (PoV1), respectively. In particular, the amino acid sequence of 2.5
kb dsRNA segment had high identity with the conserved motifs A to C in RDRP of PoV1, a
Partiviridae virus. After eliminating the viruses in
P. ostreatus TD300, the symptoms of degeneration completely disappeared. The results reveal that
P. ostreatus TD300 was at least infected by a particle virus POSV, and two naked viruses, one was a dsRNA virus with a 2.0
kb dsRNA segment, the other was an ssRNA virus whose replicating form of genome was an 8.2
kb dsRNA segment. Mycoviruses infection is a causative agent of mushroom strain degeneration.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>20943162</pmid><doi>10.1016/j.funbio.2010.04.001</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1878-6146 |
| ispartof | Fungal biology, 2010-05, Vol.114 (5), p.507-513 |
| issn | 1878-6146 1878-6162 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino acid sequence Base Sequence Industrial Microbiology Molecular Sequence Data Naked virus Particle virus Pleurotus - growth & development Pleurotus - virology Pleurotus ostreatus RNA mycovirus RNA Viruses - genetics RNA Viruses - isolation & purification RNA Viruses - physiology RNA Viruses - ultrastructure RNA, Viral - genetics Sequence Alignment Strain degeneration Viral Proteins - genetics Virion - genetics Virion - isolation & purification Virion - physiology Virion - ultrastructure |
| title | Particle and naked RNA mycoviruses in industrially cultivated mushroom Pleurotus ostreatus in China |
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