Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA
Transcriptome analyses in eukaryotes, including mice and humans, have identified polyA-containing transcripts that lack long open reading frames (ORFs; >100 amino acids). These transcripts are believed most likely to function as non-coding RNAs, but their translational capacities and biological a...
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Veröffentlicht in: | Nature cell biology 2007-06, Vol.9 (6), p.660-665 |
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creator | Kageyama, Yuji Kondo, Takefumi Hashimoto, Yoshiko Kato, Kagayaki Inagaki, Sachi Hayashi, Shigeo |
description | Transcriptome analyses in eukaryotes, including mice and humans, have identified polyA-containing transcripts that lack long open reading frames (ORFs; >100 amino acids). These transcripts are believed most likely to function as non-coding RNAs, but their translational capacities and biological activities have not been characterized in detail. Here, we report that polished rice (pri), which was previously identified as a gene for a non-coding RNA in Drosophila, is in fact transcribed into a polycistronic mRNA that contains evolutionarily conserved short ORFs that encode 11 or 32 amino acid-long peptides. pri was expressed in all epithelial tissues during embryogenesis. The loss of pri function completely eliminated apical cuticular structures, including the epidermal denticles and tracheal taenidia, and also caused defective tracheal-tube expansion. We found that pri is essential for the formation of specific F-actin bundles that prefigures the formation of the denticles and taenidium. We provide evidences that pri acts non-cell autonomously and that four of the conserved pri ORFs are functionally redundant. These results demonstrate that pri has essential roles in epithelial morphogenesis by regulating F-actin organization. |
doi_str_mv | 10.1038/ncb1595 |
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These results demonstrate that pri has essential roles in epithelial morphogenesis by regulating F-actin organization.</description><identifier>ISSN: 1465-7392</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/ncb1595</identifier><identifier>PMID: 17486114</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Actin ; Actins - metabolism ; Amino acids ; Animals ; Base Sequence ; Biomedical and Life Sciences ; Cancer Research ; Cell Biology ; Cell Differentiation - genetics ; Cells ; Conserved Sequence - genetics ; Developmental Biology ; Drosophila ; Drosophila - cytology ; Drosophila - embryology ; Drosophila - metabolism ; Embryonic Development - genetics ; Embryonic growth stage ; Epithelium - embryology ; Epithelium - metabolism ; Evolution, Molecular ; Gene Expression Regulation, Developmental - genetics ; Genes ; Genes - genetics ; Genetic aspects ; Genomes ; Insects ; letter ; Life Sciences ; Messenger RNA ; Molecular Sequence Data ; Morphogenesis ; Open Reading Frames - genetics ; Oryza sativa ; Peptides ; Peptides - genetics ; Peptides - metabolism ; Physiological aspects ; RNA, Messenger - genetics ; Science ; Sequence Homology, Nucleic Acid ; Stem Cells ; Taenidia</subject><ispartof>Nature cell biology, 2007-06, Vol.9 (6), p.660-665</ispartof><rights>Springer Nature Limited 2007</rights><rights>COPYRIGHT 2007 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jun 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c591t-4929587efd18e2309b490114539a703cd7cc02eb4df9c8c4ffa112c60c91b7283</citedby><cites>FETCH-LOGICAL-c591t-4929587efd18e2309b490114539a703cd7cc02eb4df9c8c4ffa112c60c91b7283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/ncb1595$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/ncb1595$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,2727,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17486114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kageyama, Yuji</creatorcontrib><creatorcontrib>Kondo, Takefumi</creatorcontrib><creatorcontrib>Hashimoto, Yoshiko</creatorcontrib><creatorcontrib>Kato, Kagayaki</creatorcontrib><creatorcontrib>Inagaki, Sachi</creatorcontrib><creatorcontrib>Hayashi, Shigeo</creatorcontrib><title>Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA</title><title>Nature cell biology</title><addtitle>Nat Cell Biol</addtitle><addtitle>Nat Cell Biol</addtitle><description>Transcriptome analyses in eukaryotes, including mice and humans, have identified polyA-containing transcripts that lack long open reading frames (ORFs; >100 amino acids). These transcripts are believed most likely to function as non-coding RNAs, but their translational capacities and biological activities have not been characterized in detail. Here, we report that polished rice (pri), which was previously identified as a gene for a non-coding RNA in Drosophila, is in fact transcribed into a polycistronic mRNA that contains evolutionarily conserved short ORFs that encode 11 or 32 amino acid-long peptides. pri was expressed in all epithelial tissues during embryogenesis. The loss of pri function completely eliminated apical cuticular structures, including the epidermal denticles and tracheal taenidia, and also caused defective tracheal-tube expansion. We found that pri is essential for the formation of specific F-actin bundles that prefigures the formation of the denticles and taenidium. We provide evidences that pri acts non-cell autonomously and that four of the conserved pri ORFs are functionally redundant. These results demonstrate that pri has essential roles in epithelial morphogenesis by regulating F-actin organization.</description><subject>Actin</subject><subject>Actins - metabolism</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer Research</subject><subject>Cell Biology</subject><subject>Cell Differentiation - genetics</subject><subject>Cells</subject><subject>Conserved Sequence - genetics</subject><subject>Developmental Biology</subject><subject>Drosophila</subject><subject>Drosophila - cytology</subject><subject>Drosophila - embryology</subject><subject>Drosophila - metabolism</subject><subject>Embryonic Development - genetics</subject><subject>Embryonic growth stage</subject><subject>Epithelium - embryology</subject><subject>Epithelium - metabolism</subject><subject>Evolution, Molecular</subject><subject>Gene Expression Regulation, Developmental - genetics</subject><subject>Genes</subject><subject>Genes - 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Academic</collection><jtitle>Nature cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kageyama, Yuji</au><au>Kondo, Takefumi</au><au>Hashimoto, Yoshiko</au><au>Kato, Kagayaki</au><au>Inagaki, Sachi</au><au>Hayashi, Shigeo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA</atitle><jtitle>Nature cell biology</jtitle><stitle>Nat Cell Biol</stitle><addtitle>Nat Cell Biol</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>9</volume><issue>6</issue><spage>660</spage><epage>665</epage><pages>660-665</pages><issn>1465-7392</issn><eissn>1476-4679</eissn><abstract>Transcriptome analyses in eukaryotes, including mice and humans, have identified polyA-containing transcripts that lack long open reading frames (ORFs; >100 amino acids). These transcripts are believed most likely to function as non-coding RNAs, but their translational capacities and biological activities have not been characterized in detail. Here, we report that polished rice (pri), which was previously identified as a gene for a non-coding RNA in Drosophila, is in fact transcribed into a polycistronic mRNA that contains evolutionarily conserved short ORFs that encode 11 or 32 amino acid-long peptides. pri was expressed in all epithelial tissues during embryogenesis. The loss of pri function completely eliminated apical cuticular structures, including the epidermal denticles and tracheal taenidia, and also caused defective tracheal-tube expansion. We found that pri is essential for the formation of specific F-actin bundles that prefigures the formation of the denticles and taenidium. We provide evidences that pri acts non-cell autonomously and that four of the conserved pri ORFs are functionally redundant. These results demonstrate that pri has essential roles in epithelial morphogenesis by regulating F-actin organization.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>17486114</pmid><doi>10.1038/ncb1595</doi><tpages>6</tpages></addata></record> |
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subjects | Actin Actins - metabolism Amino acids Animals Base Sequence Biomedical and Life Sciences Cancer Research Cell Biology Cell Differentiation - genetics Cells Conserved Sequence - genetics Developmental Biology Drosophila Drosophila - cytology Drosophila - embryology Drosophila - metabolism Embryonic Development - genetics Embryonic growth stage Epithelium - embryology Epithelium - metabolism Evolution, Molecular Gene Expression Regulation, Developmental - genetics Genes Genes - genetics Genetic aspects Genomes Insects letter Life Sciences Messenger RNA Molecular Sequence Data Morphogenesis Open Reading Frames - genetics Oryza sativa Peptides Peptides - genetics Peptides - metabolism Physiological aspects RNA, Messenger - genetics Science Sequence Homology, Nucleic Acid Stem Cells Taenidia |
title | Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA |
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