Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA

Transcriptome analyses in eukaryotes, including mice and humans, have identified polyA-containing transcripts that lack long open reading frames (ORFs; >100 amino acids). These transcripts are believed most likely to function as non-coding RNAs, but their translational capacities and biological a...

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Veröffentlicht in:Nature cell biology 2007-06, Vol.9 (6), p.660-665
Hauptverfasser: Kageyama, Yuji, Kondo, Takefumi, Hashimoto, Yoshiko, Kato, Kagayaki, Inagaki, Sachi, Hayashi, Shigeo
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container_issue 6
container_start_page 660
container_title Nature cell biology
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creator Kageyama, Yuji
Kondo, Takefumi
Hashimoto, Yoshiko
Kato, Kagayaki
Inagaki, Sachi
Hayashi, Shigeo
description Transcriptome analyses in eukaryotes, including mice and humans, have identified polyA-containing transcripts that lack long open reading frames (ORFs; >100 amino acids). These transcripts are believed most likely to function as non-coding RNAs, but their translational capacities and biological activities have not been characterized in detail. Here, we report that polished rice (pri), which was previously identified as a gene for a non-coding RNA in Drosophila, is in fact transcribed into a polycistronic mRNA that contains evolutionarily conserved short ORFs that encode 11 or 32 amino acid-long peptides. pri was expressed in all epithelial tissues during embryogenesis. The loss of pri function completely eliminated apical cuticular structures, including the epidermal denticles and tracheal taenidia, and also caused defective tracheal-tube expansion. We found that pri is essential for the formation of specific F-actin bundles that prefigures the formation of the denticles and taenidium. We provide evidences that pri acts non-cell autonomously and that four of the conserved pri ORFs are functionally redundant. These results demonstrate that pri has essential roles in epithelial morphogenesis by regulating F-actin organization.
doi_str_mv 10.1038/ncb1595
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subjects Actin
Actins - metabolism
Amino acids
Animals
Base Sequence
Biomedical and Life Sciences
Cancer Research
Cell Biology
Cell Differentiation - genetics
Cells
Conserved Sequence - genetics
Developmental Biology
Drosophila
Drosophila - cytology
Drosophila - embryology
Drosophila - metabolism
Embryonic Development - genetics
Embryonic growth stage
Epithelium - embryology
Epithelium - metabolism
Evolution, Molecular
Gene Expression Regulation, Developmental - genetics
Genes
Genes - genetics
Genetic aspects
Genomes
Insects
letter
Life Sciences
Messenger RNA
Molecular Sequence Data
Morphogenesis
Open Reading Frames - genetics
Oryza sativa
Peptides
Peptides - genetics
Peptides - metabolism
Physiological aspects
RNA, Messenger - genetics
Science
Sequence Homology, Nucleic Acid
Stem Cells
Taenidia
title Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA
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