Regulation of activity of the transcription factor GATA-1 by acetylation
Modification of histones, DNA-binding proteins found in chromatin, by addition of acetyl groups occurs to a greater degree when the histones are associated with transcriptionally active DNA 1 , 2 . A breakthrough in understanding how this acetylation is mediated was the discovery that various transc...
Gespeichert in:
| Veröffentlicht in: | Nature (London) 1998-12, Vol.396 (6711), p.594-598 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 598 |
|---|---|
| container_issue | 6711 |
| container_start_page | 594 |
| container_title | Nature (London) |
| container_volume | 396 |
| creator | Boyes, Joan Byfield, Peter Nakatani, Yoshihiro Ogryzko, Vasily |
| description | Modification of histones, DNA-binding proteins found in chromatin, by addition of acetyl groups occurs to a greater degree when the histones are associated with transcriptionally active DNA
1
,
2
. A breakthrough in understanding how this acetylation is mediated was the discovery that various transcriptional co-activator proteins have intrinsic histone acetyltransferase activity (for example, Gcn5p (
ref. 3
), PCAF
4
, TAF
II
250 (
ref. 5
) and p300/CBP
6
,
7
). These acetyltransferases also modify certain transcription factors (TFIIEβ, TFIIF, EKLF and p53 (
8
–
10
)). GATA-1 is an important transcription factor in the haematopoietic lineage
11
and is essential for terminal differentiation of erythrocytes and megakaryocytes
12
,
13
. It is associated
in vivo
with the acetyltransferase p300/CBP
14
. Here we report that GATA-1 is acetylated
in vitro
by p300. This significantly increases the amount of GATA-1 bound to DNA and alters the mobility of GATA-1–DNA complexes, suggestive of a conformational change in GATA-1. GATA-1 is also acetylated
in vivo
and acetylation directly stimulates GATA-1-dependent transcription. Mutagenesis of important acetylated residues shows that there is a relationship between the acetylation and
in vivo
function of GATA-1. Wepropose that acetylation of transcription factors can alter interactions between these factors and DNA and among different transcription factors, and is an integral part of transcription and differentiation processes. |
| doi_str_mv | 10.1038/25166 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_762278106</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>743741466</sourcerecordid><originalsourceid>FETCH-LOGICAL-c552t-e388cf02af60cc968d3fb2285a297f6793e52c5c609e725342dabc5e77db723f3</originalsourceid><addsrcrecordid>eNqFkVtLAzEQhYMotWp_grCIl6fV3Cd5LKJVEATR5yWbJnVlu1uTXaH_3tQWbw_6NAPnmzPDHIRGBJ8TzNQFFUTKLTQkHGTOpYJtNMSYqhwrJnfRXowvGGNBgA_QQCuhtYYhunlws742XdU2WeszY7vqreqWq757dlkXTBNtqBYfgE9yG7LJ-HGck6xcJtx1y_X0Adrxpo5utKn76On66vHyJr-7n9xeju9yKwTtcseUsh5T4yW2Vks1Zb6kVAlDNXgJmjlBrbASawdUME6nprTCAUxLoMyzfXS29l2E9rV3sSvmVbSurk3j2j4WICkFRbD8nxRcSam0_p_kDDjhcuV5-idJIL0fFCTw6Bf40vahSZ8pKOZcClCrvSdryIY2xuB8sQjV3IRlQXCxCrX4CDVxhxuzvpy76Se1STHpxxvdRGtqn0KzVfwyk5zC9-NjUpqZC183_dz3Dkdjsbc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>204465789</pqid></control><display><type>article</type><title>Regulation of activity of the transcription factor GATA-1 by acetylation</title><source>MEDLINE</source><source>Nature</source><source>SpringerLink (Online service)</source><creator>Boyes, Joan ; Byfield, Peter ; Nakatani, Yoshihiro ; Ogryzko, Vasily</creator><creatorcontrib>Boyes, Joan ; Byfield, Peter ; Nakatani, Yoshihiro ; Ogryzko, Vasily</creatorcontrib><description>Modification of histones, DNA-binding proteins found in chromatin, by addition of acetyl groups occurs to a greater degree when the histones are associated with transcriptionally active DNA
1
,
2
. A breakthrough in understanding how this acetylation is mediated was the discovery that various transcriptional co-activator proteins have intrinsic histone acetyltransferase activity (for example, Gcn5p (
ref. 3
), PCAF
4
, TAF
II
250 (
ref. 5
) and p300/CBP
6
,
7
). These acetyltransferases also modify certain transcription factors (TFIIEβ, TFIIF, EKLF and p53 (
8
–
10
)). GATA-1 is an important transcription factor in the haematopoietic lineage
11
and is essential for terminal differentiation of erythrocytes and megakaryocytes
12
,
13
. It is associated
in vivo
with the acetyltransferase p300/CBP
14
. Here we report that GATA-1 is acetylated
in vitro
by p300. This significantly increases the amount of GATA-1 bound to DNA and alters the mobility of GATA-1–DNA complexes, suggestive of a conformational change in GATA-1. GATA-1 is also acetylated
in vivo
and acetylation directly stimulates GATA-1-dependent transcription. Mutagenesis of important acetylated residues shows that there is a relationship between the acetylation and
in vivo
function of GATA-1. Wepropose that acetylation of transcription factors can alter interactions between these factors and DNA and among different transcription factors, and is an integral part of transcription and differentiation processes.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/25166</identifier><identifier>PMID: 9859997</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>3T3 Cells ; Acetylation ; Amino Acid Sequence ; Animals ; Binding Sites ; Biological and medical sciences ; Cellular biology ; Chickens ; Deoxyribonucleic acid ; DNA ; DNA - metabolism ; DNA-Binding Proteins - metabolism ; E1A-Associated p300 Protein ; Erythroid-Specific DNA-Binding Factors ; Fundamental and applied biological sciences. Psychology ; GATA1 Transcription Factor ; Humanities and Social Sciences ; letter ; Lysine - metabolism ; Mass Spectrometry ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; multidisciplinary ; Nuclear Proteins - metabolism ; Protein Binding ; Proteins ; Science ; Science (multidisciplinary) ; Trans-Activators - metabolism ; Transcription Factors - metabolism ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing ; Tumor Cells, Cultured ; Zinc Fingers</subject><ispartof>Nature (London), 1998-12, Vol.396 (6711), p.594-598</ispartof><rights>Macmillan Magazines Ltd. 1998</rights><rights>1999 INIST-CNRS</rights><rights>Copyright Macmillan Journals Ltd. Dec 10, 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c552t-e388cf02af60cc968d3fb2285a297f6793e52c5c609e725342dabc5e77db723f3</citedby><cites>FETCH-LOGICAL-c552t-e388cf02af60cc968d3fb2285a297f6793e52c5c609e725342dabc5e77db723f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/25166$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/25166$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1642766$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9859997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boyes, Joan</creatorcontrib><creatorcontrib>Byfield, Peter</creatorcontrib><creatorcontrib>Nakatani, Yoshihiro</creatorcontrib><creatorcontrib>Ogryzko, Vasily</creatorcontrib><title>Regulation of activity of the transcription factor GATA-1 by acetylation</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Modification of histones, DNA-binding proteins found in chromatin, by addition of acetyl groups occurs to a greater degree when the histones are associated with transcriptionally active DNA
1
,
2
. A breakthrough in understanding how this acetylation is mediated was the discovery that various transcriptional co-activator proteins have intrinsic histone acetyltransferase activity (for example, Gcn5p (
ref. 3
), PCAF
4
, TAF
II
250 (
ref. 5
) and p300/CBP
6
,
7
). These acetyltransferases also modify certain transcription factors (TFIIEβ, TFIIF, EKLF and p53 (
8
–
10
)). GATA-1 is an important transcription factor in the haematopoietic lineage
11
and is essential for terminal differentiation of erythrocytes and megakaryocytes
12
,
13
. It is associated
in vivo
with the acetyltransferase p300/CBP
14
. Here we report that GATA-1 is acetylated
in vitro
by p300. This significantly increases the amount of GATA-1 bound to DNA and alters the mobility of GATA-1–DNA complexes, suggestive of a conformational change in GATA-1. GATA-1 is also acetylated
in vivo
and acetylation directly stimulates GATA-1-dependent transcription. Mutagenesis of important acetylated residues shows that there is a relationship between the acetylation and
in vivo
function of GATA-1. Wepropose that acetylation of transcription factors can alter interactions between these factors and DNA and among different transcription factors, and is an integral part of transcription and differentiation processes.</description><subject>3T3 Cells</subject><subject>Acetylation</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cellular biology</subject><subject>Chickens</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>E1A-Associated p300 Protein</subject><subject>Erythroid-Specific DNA-Binding Factors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GATA1 Transcription Factor</subject><subject>Humanities and Social Sciences</subject><subject>letter</subject><subject>Lysine - metabolism</subject><subject>Mass Spectrometry</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>multidisciplinary</subject><subject>Nuclear Proteins - metabolism</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Trans-Activators - metabolism</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Tumor Cells, Cultured</subject><subject>Zinc Fingers</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkVtLAzEQhYMotWp_grCIl6fV3Cd5LKJVEATR5yWbJnVlu1uTXaH_3tQWbw_6NAPnmzPDHIRGBJ8TzNQFFUTKLTQkHGTOpYJtNMSYqhwrJnfRXowvGGNBgA_QQCuhtYYhunlws742XdU2WeszY7vqreqWq757dlkXTBNtqBYfgE9yG7LJ-HGck6xcJtx1y_X0Adrxpo5utKn76On66vHyJr-7n9xeju9yKwTtcseUsh5T4yW2Vks1Zb6kVAlDNXgJmjlBrbASawdUME6nprTCAUxLoMyzfXS29l2E9rV3sSvmVbSurk3j2j4WICkFRbD8nxRcSam0_p_kDDjhcuV5-idJIL0fFCTw6Bf40vahSZ8pKOZcClCrvSdryIY2xuB8sQjV3IRlQXCxCrX4CDVxhxuzvpy76Se1STHpxxvdRGtqn0KzVfwyk5zC9-NjUpqZC183_dz3Dkdjsbc</recordid><startdate>19981210</startdate><enddate>19981210</enddate><creator>Boyes, Joan</creator><creator>Byfield, Peter</creator><creator>Nakatani, Yoshihiro</creator><creator>Ogryzko, Vasily</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T5</scope><scope>7TG</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>R05</scope><scope>RC3</scope><scope>S0X</scope><scope>SOI</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>19981210</creationdate><title>Regulation of activity of the transcription factor GATA-1 by acetylation</title><author>Boyes, Joan ; Byfield, Peter ; Nakatani, Yoshihiro ; Ogryzko, Vasily</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c552t-e388cf02af60cc968d3fb2285a297f6793e52c5c609e725342dabc5e77db723f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>3T3 Cells</topic><topic>Acetylation</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Cellular biology</topic><topic>Chickens</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>E1A-Associated p300 Protein</topic><topic>Erythroid-Specific DNA-Binding Factors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GATA1 Transcription Factor</topic><topic>Humanities and Social Sciences</topic><topic>letter</topic><topic>Lysine - metabolism</topic><topic>Mass Spectrometry</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>multidisciplinary</topic><topic>Nuclear Proteins - metabolism</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Trans-Activators - metabolism</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Tumor Cells, Cultured</topic><topic>Zinc Fingers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boyes, Joan</creatorcontrib><creatorcontrib>Byfield, Peter</creatorcontrib><creatorcontrib>Nakatani, Yoshihiro</creatorcontrib><creatorcontrib>Ogryzko, Vasily</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database (ProQuest)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Psychology</collection><collection>ProQuest Research Library</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>test</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>University of Michigan</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>Environment Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boyes, Joan</au><au>Byfield, Peter</au><au>Nakatani, Yoshihiro</au><au>Ogryzko, Vasily</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of activity of the transcription factor GATA-1 by acetylation</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1998-12-10</date><risdate>1998</risdate><volume>396</volume><issue>6711</issue><spage>594</spage><epage>598</epage><pages>594-598</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>Modification of histones, DNA-binding proteins found in chromatin, by addition of acetyl groups occurs to a greater degree when the histones are associated with transcriptionally active DNA
1
,
2
. A breakthrough in understanding how this acetylation is mediated was the discovery that various transcriptional co-activator proteins have intrinsic histone acetyltransferase activity (for example, Gcn5p (
ref. 3
), PCAF
4
, TAF
II
250 (
ref. 5
) and p300/CBP
6
,
7
). These acetyltransferases also modify certain transcription factors (TFIIEβ, TFIIF, EKLF and p53 (
8
–
10
)). GATA-1 is an important transcription factor in the haematopoietic lineage
11
and is essential for terminal differentiation of erythrocytes and megakaryocytes
12
,
13
. It is associated
in vivo
with the acetyltransferase p300/CBP
14
. Here we report that GATA-1 is acetylated
in vitro
by p300. This significantly increases the amount of GATA-1 bound to DNA and alters the mobility of GATA-1–DNA complexes, suggestive of a conformational change in GATA-1. GATA-1 is also acetylated
in vivo
and acetylation directly stimulates GATA-1-dependent transcription. Mutagenesis of important acetylated residues shows that there is a relationship between the acetylation and
in vivo
function of GATA-1. Wepropose that acetylation of transcription factors can alter interactions between these factors and DNA and among different transcription factors, and is an integral part of transcription and differentiation processes.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>9859997</pmid><doi>10.1038/25166</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1998-12, Vol.396 (6711), p.594-598 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_762278106 |
| source | MEDLINE; Nature; SpringerLink (Online service) |
| subjects | 3T3 Cells Acetylation Amino Acid Sequence Animals Binding Sites Biological and medical sciences Cellular biology Chickens Deoxyribonucleic acid DNA DNA - metabolism DNA-Binding Proteins - metabolism E1A-Associated p300 Protein Erythroid-Specific DNA-Binding Factors Fundamental and applied biological sciences. Psychology GATA1 Transcription Factor Humanities and Social Sciences letter Lysine - metabolism Mass Spectrometry Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data multidisciplinary Nuclear Proteins - metabolism Protein Binding Proteins Science Science (multidisciplinary) Trans-Activators - metabolism Transcription Factors - metabolism Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Tumor Cells, Cultured Zinc Fingers |
| title | Regulation of activity of the transcription factor GATA-1 by acetylation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T10%3A50%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Regulation%20of%20activity%20of%20the%20transcription%20factor%20GATA-1%20by%20acetylation&rft.jtitle=Nature%20(London)&rft.au=Boyes,%20Joan&rft.date=1998-12-10&rft.volume=396&rft.issue=6711&rft.spage=594&rft.epage=598&rft.pages=594-598&rft.issn=0028-0836&rft.eissn=1476-4687&rft.coden=NATUAS&rft_id=info:doi/10.1038/25166&rft_dat=%3Cproquest_cross%3E743741466%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=204465789&rft_id=info:pmid/9859997&rfr_iscdi=true |