ΔFosB in the nucleus accumbens is critical for reinforcing effects of sexual reward
Sexual behavior in male rats is rewarding and reinforcing. However, little is known about the specific cellular and molecular mechanisms mediating sexual reward or the reinforcing effects of reward on subsequent expression of sexual behavior. This study tests the hypothesis that ΔFosB, the stably ex...
Gespeichert in:
| Veröffentlicht in: | Genes, brain and behavior brain and behavior, 2010-10, Vol.9 (7), p.831-840 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 840 |
|---|---|
| container_issue | 7 |
| container_start_page | 831 |
| container_title | Genes, brain and behavior |
| container_volume | 9 |
| creator | Pitchers, K. K. Frohmader, K. S. Vialou, V. Mouzon, E. Nestler, E. J. Lehman, M. N. Coolen, L. M. |
| description | Sexual behavior in male rats is rewarding and reinforcing. However, little is known about the specific cellular and molecular mechanisms mediating sexual reward or the reinforcing effects of reward on subsequent expression of sexual behavior. This study tests the hypothesis that ΔFosB, the stably expressed truncated form of FosB, plays a critical role in the reinforcement of sexual behavior and experience‐induced facilitation of sexual motivation and performance. Sexual experience was shown to cause ΔFosB accumulation in several limbic brain regions including the nucleus accumbens (NAc), medial prefrontal cortex, ventral tegmental area and caudate putamen but not the medial preoptic nucleus. Next, the induction of c‐Fos, a downstream (repressed) target of ΔFosB, was measured in sexually experienced and naïve animals. The number of mating‐induced c‐Fos‐immunoreactive cells was significantly decreased in sexually experienced animals compared with sexually naïve controls. Finally, ΔFosB levels and its activity in the NAc were manipulated using viral‐mediated gene transfer to study its potential role in mediating sexual experience and experience‐induced facilitation of sexual performance. Animals with ΔFosB overexpression displayed enhanced facilitation of sexual performance with sexual experience relative to controls. In contrast, the expression of ΔJunD, a dominant negative binding partner of ΔFosB, attenuated sexual experience‐induced facilitation of sexual performance and stunted long‐term maintenance of facilitation compared to green fluorescence protein and ΔFosB overexpressing groups. Together, these findings support a critical role for ΔFosB expression in the NAc for the reinforcing effects of sexual behavior and sexual experience‐induced facilitation of sexual performance. |
| doi_str_mv | 10.1111/j.1601-183X.2010.00621.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_24P</sourceid><recordid>TN_cdi_proquest_miscellaneous_762275343</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>762275343</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4171-a2a60277cc618bb344a25b8909487cebfedd6f057bcd0ddf60b525711da047823</originalsourceid><addsrcrecordid>eNqNkM1OwzAMgCMEYmPwCig3TitJmjadxIVNbCBN4jIkblGaOpCpPyNpte09eC6eiZTBzvhiy_7sRB9CmJKIhrhdRzQldEyz-DViJHQJSRmNdidoeBycHmueDdCF92tCqIgzeo4GjKShzcUQrb4-542fYlvj9h1w3ekSOo-V1l2VQ-2x9Vg721qtSmwahx3YOmRt6zcMxoBuPW4M9rDrAuFgq1xxic6MKj1c_eYRepk_rGaP4-Xz4ml2vxxrTgUdK6ZSwoTQOvwmz2POFUvybEImPBMacgNFkRqSiFwXpChMSvKEJYLSQhEuMhaP0M3h7sY1Hx34VlbWayhLVUPTeSlSxkQS8ziQ2YHUrvHegZEbZyvl9pIS2SuVa9nbkr052SuVP0rlLqxe_z7S5RUUx8U_hwG4OwBbW8L-34flYjoNRfwN3BSFcw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>762275343</pqid></control><display><type>article</type><title>ΔFosB in the nucleus accumbens is critical for reinforcing effects of sexual reward</title><source>Wiley-Blackwell Open Access Titles</source><creator>Pitchers, K. K. ; Frohmader, K. S. ; Vialou, V. ; Mouzon, E. ; Nestler, E. J. ; Lehman, M. N. ; Coolen, L. M.</creator><creatorcontrib>Pitchers, K. K. ; Frohmader, K. S. ; Vialou, V. ; Mouzon, E. ; Nestler, E. J. ; Lehman, M. N. ; Coolen, L. M.</creatorcontrib><description>Sexual behavior in male rats is rewarding and reinforcing. However, little is known about the specific cellular and molecular mechanisms mediating sexual reward or the reinforcing effects of reward on subsequent expression of sexual behavior. This study tests the hypothesis that ΔFosB, the stably expressed truncated form of FosB, plays a critical role in the reinforcement of sexual behavior and experience‐induced facilitation of sexual motivation and performance. Sexual experience was shown to cause ΔFosB accumulation in several limbic brain regions including the nucleus accumbens (NAc), medial prefrontal cortex, ventral tegmental area and caudate putamen but not the medial preoptic nucleus. Next, the induction of c‐Fos, a downstream (repressed) target of ΔFosB, was measured in sexually experienced and naïve animals. The number of mating‐induced c‐Fos‐immunoreactive cells was significantly decreased in sexually experienced animals compared with sexually naïve controls. Finally, ΔFosB levels and its activity in the NAc were manipulated using viral‐mediated gene transfer to study its potential role in mediating sexual experience and experience‐induced facilitation of sexual performance. Animals with ΔFosB overexpression displayed enhanced facilitation of sexual performance with sexual experience relative to controls. In contrast, the expression of ΔJunD, a dominant negative binding partner of ΔFosB, attenuated sexual experience‐induced facilitation of sexual performance and stunted long‐term maintenance of facilitation compared to green fluorescence protein and ΔFosB overexpressing groups. Together, these findings support a critical role for ΔFosB expression in the NAc for the reinforcing effects of sexual behavior and sexual experience‐induced facilitation of sexual performance.</description><identifier>ISSN: 1601-1848</identifier><identifier>EISSN: 1601-183X</identifier><identifier>DOI: 10.1111/j.1601-183X.2010.00621.x</identifier><identifier>PMID: 20618447</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Anterior cingulate area ; Brain Chemistry - genetics ; Brain Chemistry - physiology ; Female ; Fluorescent Antibody Technique ; Gene Expression ; Genetic Vectors ; Immunoenzyme Techniques ; learning ; Male ; Motivation - genetics ; Motivation - physiology ; neuroplasticity ; Nucleus Accumbens - anatomy & histology ; Nucleus Accumbens - metabolism ; Nucleus Accumbens - physiology ; Proto-Oncogene Proteins c-fos - metabolism ; Rats ; Rats, Sprague-Dawley ; recombinant adeno‐associated viral vector ; Reinforcement (Psychology) ; Reward ; sensitization ; sexual behavior ; Sexual Behavior, Animal - physiology</subject><ispartof>Genes, brain and behavior, 2010-10, Vol.9 (7), p.831-840</ispartof><rights>2010 The Authors. Genes, Brain and Behavior © 2010 Blackwell Publishing Ltd and International Behavioural and Neural Genetics Society</rights><rights>2010 The Authors. Genes, Brain and Behavior © 2010 Blackwell Publishing Ltd and International Behavioural and Neural Genetics Society.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4171-a2a60277cc618bb344a25b8909487cebfedd6f057bcd0ddf60b525711da047823</citedby><cites>FETCH-LOGICAL-c4171-a2a60277cc618bb344a25b8909487cebfedd6f057bcd0ddf60b525711da047823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1601-183X.2010.00621.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1601-183X.2010.00621.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,11562,27924,27925,45574,45575,46052,46476</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1601-183X.2010.00621.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20618447$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pitchers, K. K.</creatorcontrib><creatorcontrib>Frohmader, K. S.</creatorcontrib><creatorcontrib>Vialou, V.</creatorcontrib><creatorcontrib>Mouzon, E.</creatorcontrib><creatorcontrib>Nestler, E. J.</creatorcontrib><creatorcontrib>Lehman, M. N.</creatorcontrib><creatorcontrib>Coolen, L. M.</creatorcontrib><title>ΔFosB in the nucleus accumbens is critical for reinforcing effects of sexual reward</title><title>Genes, brain and behavior</title><addtitle>Genes Brain Behav</addtitle><description>Sexual behavior in male rats is rewarding and reinforcing. However, little is known about the specific cellular and molecular mechanisms mediating sexual reward or the reinforcing effects of reward on subsequent expression of sexual behavior. This study tests the hypothesis that ΔFosB, the stably expressed truncated form of FosB, plays a critical role in the reinforcement of sexual behavior and experience‐induced facilitation of sexual motivation and performance. Sexual experience was shown to cause ΔFosB accumulation in several limbic brain regions including the nucleus accumbens (NAc), medial prefrontal cortex, ventral tegmental area and caudate putamen but not the medial preoptic nucleus. Next, the induction of c‐Fos, a downstream (repressed) target of ΔFosB, was measured in sexually experienced and naïve animals. The number of mating‐induced c‐Fos‐immunoreactive cells was significantly decreased in sexually experienced animals compared with sexually naïve controls. Finally, ΔFosB levels and its activity in the NAc were manipulated using viral‐mediated gene transfer to study its potential role in mediating sexual experience and experience‐induced facilitation of sexual performance. Animals with ΔFosB overexpression displayed enhanced facilitation of sexual performance with sexual experience relative to controls. In contrast, the expression of ΔJunD, a dominant negative binding partner of ΔFosB, attenuated sexual experience‐induced facilitation of sexual performance and stunted long‐term maintenance of facilitation compared to green fluorescence protein and ΔFosB overexpressing groups. Together, these findings support a critical role for ΔFosB expression in the NAc for the reinforcing effects of sexual behavior and sexual experience‐induced facilitation of sexual performance.</description><subject>Animals</subject><subject>Anterior cingulate area</subject><subject>Brain Chemistry - genetics</subject><subject>Brain Chemistry - physiology</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Expression</subject><subject>Genetic Vectors</subject><subject>Immunoenzyme Techniques</subject><subject>learning</subject><subject>Male</subject><subject>Motivation - genetics</subject><subject>Motivation - physiology</subject><subject>neuroplasticity</subject><subject>Nucleus Accumbens - anatomy & histology</subject><subject>Nucleus Accumbens - metabolism</subject><subject>Nucleus Accumbens - physiology</subject><subject>Proto-Oncogene Proteins c-fos - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>recombinant adeno‐associated viral vector</subject><subject>Reinforcement (Psychology)</subject><subject>Reward</subject><subject>sensitization</subject><subject>sexual behavior</subject><subject>Sexual Behavior, Animal - physiology</subject><issn>1601-1848</issn><issn>1601-183X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1OwzAMgCMEYmPwCig3TitJmjadxIVNbCBN4jIkblGaOpCpPyNpte09eC6eiZTBzvhiy_7sRB9CmJKIhrhdRzQldEyz-DViJHQJSRmNdidoeBycHmueDdCF92tCqIgzeo4GjKShzcUQrb4-542fYlvj9h1w3ekSOo-V1l2VQ-2x9Vg721qtSmwahx3YOmRt6zcMxoBuPW4M9rDrAuFgq1xxic6MKj1c_eYRepk_rGaP4-Xz4ml2vxxrTgUdK6ZSwoTQOvwmz2POFUvybEImPBMacgNFkRqSiFwXpChMSvKEJYLSQhEuMhaP0M3h7sY1Hx34VlbWayhLVUPTeSlSxkQS8ziQ2YHUrvHegZEbZyvl9pIS2SuVa9nbkr052SuVP0rlLqxe_z7S5RUUx8U_hwG4OwBbW8L-34flYjoNRfwN3BSFcw</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Pitchers, K. K.</creator><creator>Frohmader, K. S.</creator><creator>Vialou, V.</creator><creator>Mouzon, E.</creator><creator>Nestler, E. J.</creator><creator>Lehman, M. N.</creator><creator>Coolen, L. M.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201010</creationdate><title>ΔFosB in the nucleus accumbens is critical for reinforcing effects of sexual reward</title><author>Pitchers, K. K. ; Frohmader, K. S. ; Vialou, V. ; Mouzon, E. ; Nestler, E. J. ; Lehman, M. N. ; Coolen, L. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4171-a2a60277cc618bb344a25b8909487cebfedd6f057bcd0ddf60b525711da047823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Anterior cingulate area</topic><topic>Brain Chemistry - genetics</topic><topic>Brain Chemistry - physiology</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Gene Expression</topic><topic>Genetic Vectors</topic><topic>Immunoenzyme Techniques</topic><topic>learning</topic><topic>Male</topic><topic>Motivation - genetics</topic><topic>Motivation - physiology</topic><topic>neuroplasticity</topic><topic>Nucleus Accumbens - anatomy & histology</topic><topic>Nucleus Accumbens - metabolism</topic><topic>Nucleus Accumbens - physiology</topic><topic>Proto-Oncogene Proteins c-fos - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>recombinant adeno‐associated viral vector</topic><topic>Reinforcement (Psychology)</topic><topic>Reward</topic><topic>sensitization</topic><topic>sexual behavior</topic><topic>Sexual Behavior, Animal - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pitchers, K. K.</creatorcontrib><creatorcontrib>Frohmader, K. S.</creatorcontrib><creatorcontrib>Vialou, V.</creatorcontrib><creatorcontrib>Mouzon, E.</creatorcontrib><creatorcontrib>Nestler, E. J.</creatorcontrib><creatorcontrib>Lehman, M. N.</creatorcontrib><creatorcontrib>Coolen, L. M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genes, brain and behavior</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Pitchers, K. K.</au><au>Frohmader, K. S.</au><au>Vialou, V.</au><au>Mouzon, E.</au><au>Nestler, E. J.</au><au>Lehman, M. N.</au><au>Coolen, L. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ΔFosB in the nucleus accumbens is critical for reinforcing effects of sexual reward</atitle><jtitle>Genes, brain and behavior</jtitle><addtitle>Genes Brain Behav</addtitle><date>2010-10</date><risdate>2010</risdate><volume>9</volume><issue>7</issue><spage>831</spage><epage>840</epage><pages>831-840</pages><issn>1601-1848</issn><eissn>1601-183X</eissn><abstract>Sexual behavior in male rats is rewarding and reinforcing. However, little is known about the specific cellular and molecular mechanisms mediating sexual reward or the reinforcing effects of reward on subsequent expression of sexual behavior. This study tests the hypothesis that ΔFosB, the stably expressed truncated form of FosB, plays a critical role in the reinforcement of sexual behavior and experience‐induced facilitation of sexual motivation and performance. Sexual experience was shown to cause ΔFosB accumulation in several limbic brain regions including the nucleus accumbens (NAc), medial prefrontal cortex, ventral tegmental area and caudate putamen but not the medial preoptic nucleus. Next, the induction of c‐Fos, a downstream (repressed) target of ΔFosB, was measured in sexually experienced and naïve animals. The number of mating‐induced c‐Fos‐immunoreactive cells was significantly decreased in sexually experienced animals compared with sexually naïve controls. Finally, ΔFosB levels and its activity in the NAc were manipulated using viral‐mediated gene transfer to study its potential role in mediating sexual experience and experience‐induced facilitation of sexual performance. Animals with ΔFosB overexpression displayed enhanced facilitation of sexual performance with sexual experience relative to controls. In contrast, the expression of ΔJunD, a dominant negative binding partner of ΔFosB, attenuated sexual experience‐induced facilitation of sexual performance and stunted long‐term maintenance of facilitation compared to green fluorescence protein and ΔFosB overexpressing groups. Together, these findings support a critical role for ΔFosB expression in the NAc for the reinforcing effects of sexual behavior and sexual experience‐induced facilitation of sexual performance.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20618447</pmid><doi>10.1111/j.1601-183X.2010.00621.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 1601-1848 |
| ispartof | Genes, brain and behavior, 2010-10, Vol.9 (7), p.831-840 |
| issn | 1601-1848 1601-183X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_762275343 |
| source | Wiley-Blackwell Open Access Titles |
| subjects | Animals Anterior cingulate area Brain Chemistry - genetics Brain Chemistry - physiology Female Fluorescent Antibody Technique Gene Expression Genetic Vectors Immunoenzyme Techniques learning Male Motivation - genetics Motivation - physiology neuroplasticity Nucleus Accumbens - anatomy & histology Nucleus Accumbens - metabolism Nucleus Accumbens - physiology Proto-Oncogene Proteins c-fos - metabolism Rats Rats, Sprague-Dawley recombinant adeno‐associated viral vector Reinforcement (Psychology) Reward sensitization sexual behavior Sexual Behavior, Animal - physiology |
| title | ΔFosB in the nucleus accumbens is critical for reinforcing effects of sexual reward |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T12%3A03%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_24P&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%CE%94FosB%20in%20the%20nucleus%20accumbens%20is%20critical%20for%20reinforcing%20effects%20of%20sexual%20reward&rft.jtitle=Genes,%20brain%20and%20behavior&rft.au=Pitchers,%20K.%20K.&rft.date=2010-10&rft.volume=9&rft.issue=7&rft.spage=831&rft.epage=840&rft.pages=831-840&rft.issn=1601-1848&rft.eissn=1601-183X&rft_id=info:doi/10.1111/j.1601-183X.2010.00621.x&rft_dat=%3Cproquest_24P%3E762275343%3C/proquest_24P%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=762275343&rft_id=info:pmid/20618447&rfr_iscdi=true |