Homogeneous amperometric immunoassay for theophylline in whole blood
A homogeneous spectrophotometric EMIT immunoassay kit for the quantitation of theophylline in serum or plasma has been modified to produce a rapid, amperometric immunoassay requiring a 50 μl whole blood sample. The basis of the detection system for the assay is the electrochemical oxidation of NADH...
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| Veröffentlicht in: | Biosensors & bioelectronics 1993, Vol.8 (9), p.415-419 |
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| creator | Athey, D. McNeil, C.J. Bailey, W.R. Hager, H.J. Mullen, W.H. Russell, L.J. |
| description | A homogeneous spectrophotometric EMIT immunoassay kit for the quantitation of theophylline in serum or plasma has been modified to produce a rapid, amperometric immunoassay requiring a 50 μl whole blood sample. The basis of the detection system for the assay is the electrochemical oxidation of NADH produced by G6PDH-labelled theophylline at a potential of + 150 mV vs Ag/AgCl using platinised activated carbon (PACE) electrodes. Comparison of the amperometric whole blood method with the conventional spectrophotometric plasma assay produced a reasonable correlation: y = 0·90
x − 1·01, (r = 0·98, n = 12). The advantage of the new method is that simple and robust instrumentation can rapidly determine theophylline in whole blood with no sample pre-treatment or separation steps. |
| doi_str_mv | 10.1016/0956-5663(93)80026-L |
| format | Article |
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x − 1·01, (r = 0·98, n = 12). The advantage of the new method is that simple and robust instrumentation can rapidly determine theophylline in whole blood with no sample pre-treatment or separation steps.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Blood Chemical Analysis - instrumentation</subject><subject>Blood Chemical Analysis - methods</subject><subject>Electronics, Medical</subject><subject>EMIT</subject><subject>Enzyme Multiplied Immunoassay Technique - instrumentation</subject><subject>Evaluation Studies as Topic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>homogeneous amperometric immunoassay</subject><subject>Humans</subject><subject>Methods. Procedures. Technologies</subject><subject>NAD</subject><subject>NADH</subject><subject>Oxidation-Reduction</subject><subject>platinised activated carbon</subject><subject>Spectrophotometry</subject><subject>theophylline</subject><subject>Theophylline - blood</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEUhYMotT7-gcIsRHQxmsckk9kIUh8VCm50HdLkxkZmJjWZKv33Th90qau7ON85XD6Ezgi-IZiIW1xxkXMh2FXFriXGVOSTPTQksmR5QRnfR8MdcoiOUvrEGJekwgM0kIyQiskhehiHJnxAC2GRMt3MIYYGuuhN5ptm0Qadkl5mLsSsm0GYz5Z17VvIfJv9zEIN2bQOwZ6gA6frBKfbe4zenx7fRuN88vr8Mrqf5KbgrMudLriclloSa5wT1AoNGlMsrSSCg-DEiaml3EluqWWMQwlF0bfAFVBIwo7R5WZ3HsPXAlKnGp8M1LVe_69KQWnJCf0XJKKUjIrVYrEBTQwpRXBqHn2j41IRrFaW1UqhWilUFVNry2rS1863-4tpA3ZX2mrt84ttrpPRtYu6NT7tMFZxSnnVY3cbDHpp3x6iSsZDa8D6CKZTNvi___gF7w6ZcA</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Athey, D.</creator><creator>McNeil, C.J.</creator><creator>Bailey, W.R.</creator><creator>Hager, H.J.</creator><creator>Mullen, W.H.</creator><creator>Russell, L.J.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Homogeneous amperometric immunoassay for theophylline in whole blood</title><author>Athey, D. ; McNeil, C.J. ; Bailey, W.R. ; Hager, H.J. ; Mullen, W.H. ; Russell, L.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-fa458b7a81dcff62d6aea0208d8165e651f6bd25f85d2d335e7e44fa4ef4e4813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Blood Chemical Analysis - instrumentation</topic><topic>Blood Chemical Analysis - methods</topic><topic>Electronics, Medical</topic><topic>EMIT</topic><topic>Enzyme Multiplied Immunoassay Technique - instrumentation</topic><topic>Evaluation Studies as Topic</topic><topic>Fundamental and applied biological sciences. 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Technologies</topic><topic>NAD</topic><topic>NADH</topic><topic>Oxidation-Reduction</topic><topic>platinised activated carbon</topic><topic>Spectrophotometry</topic><topic>theophylline</topic><topic>Theophylline - blood</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Athey, D.</creatorcontrib><creatorcontrib>McNeil, C.J.</creatorcontrib><creatorcontrib>Bailey, W.R.</creatorcontrib><creatorcontrib>Hager, H.J.</creatorcontrib><creatorcontrib>Mullen, W.H.</creatorcontrib><creatorcontrib>Russell, L.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Athey, D.</au><au>McNeil, C.J.</au><au>Bailey, W.R.</au><au>Hager, H.J.</au><au>Mullen, W.H.</au><au>Russell, L.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Homogeneous amperometric immunoassay for theophylline in whole blood</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>1993</date><risdate>1993</risdate><volume>8</volume><issue>9</issue><spage>415</spage><epage>419</epage><pages>415-419</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A homogeneous spectrophotometric EMIT immunoassay kit for the quantitation of theophylline in serum or plasma has been modified to produce a rapid, amperometric immunoassay requiring a 50 μl whole blood sample. 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| ispartof | Biosensors & bioelectronics, 1993, Vol.8 (9), p.415-419 |
| issn | 0956-5663 1873-4235 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Blood Chemical Analysis - instrumentation Blood Chemical Analysis - methods Electronics, Medical EMIT Enzyme Multiplied Immunoassay Technique - instrumentation Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology homogeneous amperometric immunoassay Humans Methods. Procedures. Technologies NAD NADH Oxidation-Reduction platinised activated carbon Spectrophotometry theophylline Theophylline - blood Various methods and equipments |
| title | Homogeneous amperometric immunoassay for theophylline in whole blood |
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