Mass spectrometry of murine leukemia virus core proteins
A mass spectrometry (MS) approach was used to analyze viral core proteins of the murine leukemia virus (MuLV)-based gene delivery vector. The retroviral particles produced by traditional methods were concentrated and purified by ultracentrifugation and spin column for matrix-assisted laser desorptio...
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| Veröffentlicht in: | Journal of virological methods 2010-11, Vol.169 (2), p.290-295 |
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| container_title | Journal of virological methods |
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| creator | Medvedkin, Viacheslav Moore, Roger Falick, Arnold M. Conner, John Omelchenko, Tatiana Young, Mary Anderson, W. French Golubkov, Vladislav S. Rozenberg-Adler, Yanina |
| description | A mass spectrometry (MS) approach was used to analyze viral core proteins of the murine leukemia virus (MuLV)-based gene delivery vector. The retroviral particles produced by traditional methods were concentrated and purified by ultracentrifugation and spin column for matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) MS. MALDI application detected all core MuLV proteins, partial degradation of p10, phosphorylation of p12, as well as the previously unknown formation of a polymeric supramolecular complex between p15 and p30 core proteins. ESI provided information on the post-translational modifications of MuLV core proteins. Data suggest myristoylation of p15 and oxidation of methionine residues in both p12 and p30, whereas cysteine residues in p10, p15 and p30 were not oxidized. The current study demonstrates that MALDI and ESI are efficient tools for viral core protein analysis and can be used as analytical tools in virology and biotechnology of gene delivery vectors. |
| doi_str_mv | 10.1016/j.jviromet.2010.07.028 |
| format | Article |
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French ; Golubkov, Vladislav S. ; Rozenberg-Adler, Yanina</creator><creatorcontrib>Medvedkin, Viacheslav ; Moore, Roger ; Falick, Arnold M. ; Conner, John ; Omelchenko, Tatiana ; Young, Mary ; Anderson, W. French ; Golubkov, Vladislav S. ; Rozenberg-Adler, Yanina</creatorcontrib><description>A mass spectrometry (MS) approach was used to analyze viral core proteins of the murine leukemia virus (MuLV)-based gene delivery vector. The retroviral particles produced by traditional methods were concentrated and purified by ultracentrifugation and spin column for matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) MS. MALDI application detected all core MuLV proteins, partial degradation of p10, phosphorylation of p12, as well as the previously unknown formation of a polymeric supramolecular complex between p15 and p30 core proteins. ESI provided information on the post-translational modifications of MuLV core proteins. Data suggest myristoylation of p15 and oxidation of methionine residues in both p12 and p30, whereas cysteine residues in p10, p15 and p30 were not oxidized. The current study demonstrates that MALDI and ESI are efficient tools for viral core protein analysis and can be used as analytical tools in virology and biotechnology of gene delivery vectors.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2010.07.028</identifier><identifier>PMID: 20691207</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Biological and medical sciences ; Core proteins ; Fundamental and applied biological sciences. Psychology ; Gene therapy ; Leukemia Virus, Murine - chemistry ; Leukemia Virus, Murine - isolation & purification ; Mass spectrometry ; Microbiology ; MuLV ; Murine leukemia virus ; Protein Processing, Post-Translational ; Spectrometry, Mass, Electrospray Ionization ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Techniques used in virology ; Ultracentrifugation ; Viral Core Proteins - chemistry ; Viral Core Proteins - isolation & purification ; Virology</subject><ispartof>Journal of virological methods, 2010-11, Vol.169 (2), p.290-295</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010. 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French</creatorcontrib><creatorcontrib>Golubkov, Vladislav S.</creatorcontrib><creatorcontrib>Rozenberg-Adler, Yanina</creatorcontrib><title>Mass spectrometry of murine leukemia virus core proteins</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>A mass spectrometry (MS) approach was used to analyze viral core proteins of the murine leukemia virus (MuLV)-based gene delivery vector. The retroviral particles produced by traditional methods were concentrated and purified by ultracentrifugation and spin column for matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) MS. MALDI application detected all core MuLV proteins, partial degradation of p10, phosphorylation of p12, as well as the previously unknown formation of a polymeric supramolecular complex between p15 and p30 core proteins. ESI provided information on the post-translational modifications of MuLV core proteins. Data suggest myristoylation of p15 and oxidation of methionine residues in both p12 and p30, whereas cysteine residues in p10, p15 and p30 were not oxidized. The current study demonstrates that MALDI and ESI are efficient tools for viral core protein analysis and can be used as analytical tools in virology and biotechnology of gene delivery vectors.</description><subject>Biological and medical sciences</subject><subject>Core proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene therapy</subject><subject>Leukemia Virus, Murine - chemistry</subject><subject>Leukemia Virus, Murine - isolation & purification</subject><subject>Mass spectrometry</subject><subject>Microbiology</subject><subject>MuLV</subject><subject>Murine leukemia virus</subject><subject>Protein Processing, Post-Translational</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Techniques used in virology</subject><subject>Ultracentrifugation</subject><subject>Viral Core Proteins - chemistry</subject><subject>Viral Core Proteins - isolation & purification</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctOwzAQRS0EoqXwC1U2iFXL-BE72YEqXlIRG1hbjjORXPIodlKpf49LCyy7Gmt0Zu7omJAphTkFKm9X89XG-a7Bfs4gNkHNgWUnZEwzlc8gz8QpGUdQxjcXI3IRwgoAUsX5ORkxkDlloMYkezUhJGGNtv_Z5rdJVyXN4F2LSY3DJzbOJDFqCIntPCZr3_Xo2nBJzipTB7w61An5eHx4XzzPlm9PL4v75cyKPO1n1IjcWoEipYBSFWlBlTFc0cIoVbKKMiOlBVQoKZhKiaqsVMlzWUorbJHzCbnZ743BXwOGXjcuWKxr02I3BK0kY4oJnh4n0zSXmQAeSbknre9C8FjptXeN8VtNQe_06pX-1at3ejUoHfXGwekhYigaLP_Gfn1G4PoAmGBNXXnTWhf-Oc6B03R3wd2ew6hu49DrYB22Fkvn41fosnPHbvkGRyqcOQ</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Medvedkin, Viacheslav</creator><creator>Moore, Roger</creator><creator>Falick, Arnold M.</creator><creator>Conner, John</creator><creator>Omelchenko, Tatiana</creator><creator>Young, Mary</creator><creator>Anderson, W. 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French ; Golubkov, Vladislav S. ; Rozenberg-Adler, Yanina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-1a49cc4e4510e67b5b17aa371ba77d2f12a66c0e7e610af74fdf7d396d6c4cb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Core proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene therapy</topic><topic>Leukemia Virus, Murine - chemistry</topic><topic>Leukemia Virus, Murine - isolation & purification</topic><topic>Mass spectrometry</topic><topic>Microbiology</topic><topic>MuLV</topic><topic>Murine leukemia virus</topic><topic>Protein Processing, Post-Translational</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Techniques used in virology</topic><topic>Ultracentrifugation</topic><topic>Viral Core Proteins - chemistry</topic><topic>Viral Core Proteins - isolation & purification</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Medvedkin, Viacheslav</creatorcontrib><creatorcontrib>Moore, Roger</creatorcontrib><creatorcontrib>Falick, Arnold M.</creatorcontrib><creatorcontrib>Conner, John</creatorcontrib><creatorcontrib>Omelchenko, Tatiana</creatorcontrib><creatorcontrib>Young, Mary</creatorcontrib><creatorcontrib>Anderson, W. 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| ispartof | Journal of virological methods, 2010-11, Vol.169 (2), p.290-295 |
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| subjects | Biological and medical sciences Core proteins Fundamental and applied biological sciences. Psychology Gene therapy Leukemia Virus, Murine - chemistry Leukemia Virus, Murine - isolation & purification Mass spectrometry Microbiology MuLV Murine leukemia virus Protein Processing, Post-Translational Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Techniques used in virology Ultracentrifugation Viral Core Proteins - chemistry Viral Core Proteins - isolation & purification Virology |
| title | Mass spectrometry of murine leukemia virus core proteins |
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